• 한국연소학회:학술대회논문집
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• 2003.12a
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• pp.57-61
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• 2003

In the combustion modeling of non-premixed flames, the mixture fraction conserved scalar approach is widely utilized because reactants are mixed at the molecular level before burning and atomic elements are conserved in chemical reactions. In the mixture fraction approach, combustion process is simplified to a mixing problem and the interaction between chemistry and turbulence could be modelled by many sophisticated combustion models including the flamelet model and CMC. However, most of the mixture fraction approach is restricted to one mixture system. In this study, the flamelet model based on the two-feed system is extended to the multiple fuel-feeding systems by the two mixture fraction conserved scalar approach.

• Korean Journal of Microbiology
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• v.29 no.4
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• pp.209-214
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• 1991

The PRD1 DNA polymerase is a small multi-functional enzyme containing conserved amino acid sequences shared by family B DNA polymerases. Thus the PRD1 DNA polymerase provides an useful model system with which to study structure-functional relationships of DNA polymerase molecules. In order to investigate the functional and structural roles of the highly conserved amino acid sequences, we have introduced three mutations into a conserved amino acid of the PRD1 DNA polymerase. Genetic complememtation study indicated that each mutation inactivated DNA polymerase catalytic activity.

• Journal of Microbiology and Biotechnology
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• v.25 no.1
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• pp.127-136
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• 2015

Cupredoxin-like proteins are mainly copper-binding proteins that conserve a typical rigid Greek-key arrangement consisting of an eight-stranded β-sandwich, even though they share as little as 10-15% sequence similarity. The electron transport function of the Cupredoxins is critical for respiration and photosynthesis, and the proteins have therapeutic potential. Despite their crucial biological functions, the identification of the distant Cupredoxin homologs has been a difficult task due to their low sequence identity. In this study, the overlapped conserved residue (OCR) fingerprint for the Cupredoxin superfamily, which consists of conserved residues in three aspects (i.e., the sequence, structure, and intramolecular interaction), was used to detect the novel Cupredoxin homologs in the NCBI non-redundant protein sequence database. The OCR fingerprint could identify 54 potential Cupredoxin sequences, which were validated by scanning them against the conserved Cupredoxin motif near the Cu-binding site. This study also attempted to model the 3D structures and to predict the functions of the identified potential Cupredoxins. This study suggests that the OCR-based approach can be used efficiently to detect novel homologous proteins with low sequence identity, such as Cupredoxins.

• Nuclear Engineering and Technology
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• v.54 no.11
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• pp.4322-4328
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• 2022

The two-fluid model is widely used to describe two-phase flows in complex systems such as nuclear reactors. Although the two-phase flow was successfully simulated, the standard two-fluid model suffers from an ill-posed nature. There are several remedies for the ill-posedness of the one-dimensional (1D) two-fluid model; among those, artificial viscosity is the focus of this study. Some previous works added artificial diffusion terms to both mass and momentum equations to render the two-fluid model well-posed and demonstrated that this method provided a numerically converging model. However, they did not consider mass conservation, which is crucial for analyzing a closed reactor system. In fact, the total mass is not conserved in the previous models. This study improves the artificial viscosity model such that the 1D incompressible two-fluid model is well-posed, and the total mass is conserved. The water faucet and Kelvin-Helmholtz instability flows were simulated to test the effect of the proposed artificial viscosity model. The results indicate that the proposed artificial viscosity model effectively remedies the ill-posedness of the two-fluid model while maintaining a negligible total mass error.

• The Microorganisms and Industry
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• v.16 no.3
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• pp.6-14
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• 1990

PRDI DNA polymerase is the smallest member of the family B DNA polymerase (Jung et al., 1987). This DNA polyerase is specified by bacteriophage PRDI which infects a wide variety of gram-negative bacteria(Mindich and Bamford, 1988). Because PRDI is highly amenable to genetic and biochemical manipulation, it is a convenient model system with which to study structure-function relationships of DNA polymerase molecules. To determine the functional roles of the highly conserved regions of the family B DNA polymerases, we have initiated site-directed mutagenesis with PRD1 DNA polymerase, and our results show that mutations at the conserved regions within PRD1 DNA polymerase inactivate polymerase complementing activity and catalytic activity.

• Proceedings of the Korean Vacuum Society Conference
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• 2008.08a
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• pp.244-244
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• 2008

• Journal of Life Science
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• v.12 no.5
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• pp.610-621
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• 2002

Conserved genes are importantly used to understand the major function in survival and replication of living organism. This study was focused on identification of conserved genes in microbial species and measuring the degree of conservation. For this purpose, in silico analysis was performed to search conserved genes based on the conservation level within microbial species. The ortholog list of COGs (Clusters of Orthologous Groups of proteins) in NCBI was used and whole genomes of 43 microbial species were included in that list. The distance value, derived from CLUSTALW multiple alignment program, was used as a descriptor of the conservation level of orthologs. It was revealed that 43 microbial genomes hold 72 conserved orthologs in common. The majority(72.2%) of the conserved genes was related to "translation, ribosomal structure and biogenesis" functional category. A GTPase-translation elogation factor(COG0050) was the best conserved gene from the distance value analysis. The 72 conserved genes, found in this research, would be useful not only to study minimal function genes but also new drug target among pathogens and to make a model of the virtual cell.tual cell.

• Journal of Photoscience
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• v.12 no.3
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• pp.163-169
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• 2005

Recent reports suggest that floral organs such as sepals, petals, stamens, and carpels are specified by quaternary MADS protein complexes with different combinations. The formation of quaternary complexes of ABCDE MADS proteins may be the molecular basis of ABCDE model for the floral organ development. The MADS complexes involved in each floral organ development seem to be conserved in at least dicot species although detailed molecular mechanism is slightly different depending on species. Even in monocot, at least rice, MADS complexes similar to those in dicot exist, suggesting that the floral organ specification by MADS protein complexes may be conserved in most of plants. The MADS protein complexes may have more specific recognition of target genes or more transcription activation ability than monomers or dimers, resulting in finely regulated floral organ development.

• Journal of Welding and Joining
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• v.35 no.2
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• pp.13-22
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• 2017

In laser full penetration welding process, full penetration hole(FPH) is formed as a result of force balance between the vapor pressure and the surface tension of the surrounding molten metal. In this work, a three-dimensional numerical model based on a conserved-mass level-set method is developed to simulate the transport phenomena during laser full penetration welding process, including full penetration keyhole dynamics. Ray trancing model is applied to simulate multi-reflection phenomena in the keyhole wall. The ghost fluid method and continuum method are used to deal with liquid/vapor interface and solid/liquid interface. The effects of processing parameters including laser power and scanning speed on the resultant full penetration hole diameter, laser energy distribution and energy absorption efficiency are studied. The model is validated against experimental results. The diameter of full penetration hole calculated by the simulation model agrees well with the coaxial images captured during laser welding of thin stainless steel plates. Numerical simulation results show that increase of laser power and decrease of welding speed can enlarge the full penetration hole, which decreases laser energy efficiency.

• Journal of ILASS-Korea
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• v.22 no.3
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• pp.137-145
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• 2017

This paper describes numerical modeling of transcritical and supercritical fluid flows within a liquid propellant rocket engine. In the present paper, turbulence is modeled by standard $k-{\varepsilon}$ model. A conserved scalar approach in conjunction with multi-environment probability density function model is used to account for the turbulent mixing of real-fluids in the transcritical and supercritical region. The two real-fluid equations of state and dense-fluid correction schemes for mixtures are used to construct thermodynamic data library based on the conserved scalar. In this study, calculations are made on two cryogenic nitrogen jets under different chamber pressures. Sensitivity analysis for two different real-fluid equations of sate is particularly emphasized. Based on numerical results, precise structures of cryogenic nitrogen jets are discussed in detail. Numerical results show that the current real-fluid model can predict the essential features of the cryogenic liquid nitrogen jets.