• Genomics & Informatics
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• 제5권4호
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• pp.174-178
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• 2007

The earliest stages of mammalian embryogenesis are governed by the activity of maternally inherited transcripts and proteins. Cytoplasmic polyadenylation of selected maternal mRNA has been reported to be a major control mechanism of delayed translation during preimplantation embryogenesis in mice. The presence of cis-elements required for cytoplasmic polyadenylation (e.g., CPE) can serve as a useful tag in the screening of maternal genes partaking in key functions in the transcriptionally dormant egg and early embryo. However, due to its relative simplicity, UA-rich sequences satisfying the canonical rule of known CPE consensus sequences are often found in the 3'-UTR of maternal transcripts that do not actually undergo cytoplasmic polyadenylation. In this study, we developed a method to confirm the validity of candidate CPE sequences in a given gene by a multiplex comparison of 3'-UTR sequences between mammalian homologs. We found that genes undergoing cytoplasmic polyadenylation tend to create a conserved block around the CPE, while CPE-like sequences in the 3'-UTR of genes lacking cytoplasmic polyadenylation do not exhibit such conservation between species. Through this cross-species comparison, we also identified an alternative CPE in the 3'-UTR of tissue-type plasminogen activator (tPA), which is more likely to serve as a functional element. We suggest that verification of CPEs based on sequence conservation can provide a convenient tool for mass screening of factors governing the earliest processes of mammalian embryogenesis.

• Journal of Microbiology and Biotechnology
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• 제26권7호
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• pp.1163-1172
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• 2016

The Ω-loop is a nonregular and flexible structure that plays an important role in molecular recognition, protein folding, and thermostability. In the present study, molecular dynamics simulation was carried out to assess the molecular stability and flexibility profile of the porcine trypsin structures. Two Ω-Loops (fragment 57-67 and fragment 78-91) were confirmed to represent the flexible region. Subsequently, glycosylation site-directed mutations (A73S, N84S, and R104S) were introduced within the Ω-loop region and its wing chain based on its potential N-glycosylation sites (Asn-Xaa-Ser/Thr consensus sequences) and structure information to improve the thermostability of trypsin. The result demonstrated that the half-life of the N84S mutant at 50℃ increased by 177.89 min when compared with that of the wild-type enzyme. Furthermore, the significant increase in the thermal stability of the N84S mutant has also been proven by an increase in the T_m values determined by circular dichroism. Additionally, the optimum temperatures of the wild-type enzyme and the N84S mutant were 75℃ and 80℃, respectively. In conclusion, we obtained the thermostability-improved enzyme N84S mutant, and the strategy used to design this mutant based on its structural information and N-linked glycosylation modification could be applied to engineer other enzymes to meet the needs of the biotechnological industry.

• Asian-Australasian Journal of Animal Sciences
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• 제23권11호
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• pp.1399-1404
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• 2010

We have created a Bovine Genome Database, an integrated genomic resource for Bos taurus, by merging bovine data from various databases and our own data. We produced 55,213 Korean cattle (Hanwoo) ESTs from cDNA libraries from three tissues. We concentrated on genomic information based on Hanwoo transcripts and provided user-friendly search interfaces within the Bovine Genome Database. The genome browser supported alignment results for the various types of data: Hanwoo EST, consensus sequence, human gene, and predicted bovine genes. The database also provides transcript data information, gene annotation, genomic location, sequence and tissue distribution. Users can also explore bovine disease genes based on comparative mapping of homologous genes and can conduct searches centered on genes within user-selected quantitative trait loci (QTL) regions. The Bovine Genome Database can be accessed at http://bgd.nabc.go.kr.

• 척추신경추나의학회지
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• 제16권1호
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• pp.91-105
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• 2021

Objectives A case report is a detailed medical description of previously unreported observations or complications of medical conditions, unique use cases of tests, or new side effects. The purpose of this study was to evaluate the quality of case reports published in the Journal of Korea CHUNA Manual Medicine for Spine & Nerves (JKCMSN). Methods Case reports published in the JKCMSN from January 2013 to December 2020 were selected using the Oriental Medicine Advanced Searching Integrated System (OASIS). The quality of the reports was assessed based on the Consensus-based Clinical Case Reporting Guideline Development (CARE) guidelines. Results Sixty case reports that met the inclusion criteria were included in the assessment. Our analysis revealed that 71.42% of the case reports included all the necessary information and their level of reporting was good. However, their scores in the qualitative subdivisions of the reporting level parameters showed that the quality level was uneven. More than 65% of the papers did not report in the following categories: "Keywords," "Patient information," "Timeline," "Diagnostic assessment," "Therapeutic interventions," "Follow-up and outcomes," "Patient perspective," and "Informed consent." Conclusions Our analysis shows that CARE guidelines need to be followed more rigorously to improve the quality of reporting in the JKCMSN. Further efforts are necessary to develop reporting guidelines and evaluation tools relevant to the Korean clinical context.

• 한국지리정보학회지
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• 제4권2호
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• pp.1-16
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• 2001

지형정보 작업관리(워크플로) 연구는 중요한 관심이 되는 분야 중에 하나로 시간변화에 따라 발생하는 사건, 행위 그리고 프로세스들의 상호작용 및 변경 등을 처리할 수 있는 방법 및 모델이 요구된다. 기존의 많은 워크플로 프로세스 모델이 있지만, 육하원칙에 의거하여 행위자의 시간과 공간적 행위 또는 사건을 해석하려는 시도가 많지 않은 듯하다. 이 연구는 6가지의 조건을 조사하고 객체지향적인 헥사드 행위 모델을 제시하여 지형정보 프로세스 및 행위를 분석하고자 하였다. 행위자의 다양한 행위의 원인과 결과를 시공간 개념과 연계하면, 육하원칙의 자세한 분석은 공간의 변화나 공간정보 흐름을 파악하는데 상당한 도움이 된다.

• 한국정보통신학회논문지
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• 제23권2호
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• pp.229-235
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• 2019

초중등 소프트웨어교육을 운영할 수 있는 예비교사 교육과정의 중요성이 제기되고 있다. 하지만 국내에서 예비교사를 위한 소프트웨어교과교육과정에 대해서 어떤 기준에 의해 강화되어야 하는지에 대한 연구는 미흡한 실정이다. 이에 본 연구에서는 교수내용지식 4가지를 기준으로 국내외 사례를 분석하였다. 국내 사례는 교육내용과 교수법에 집중적으로 구성되어 있는 것으로 나타났다. 그리고 국내외 사례 분석을 통하여 예비교사를 위한 교수내용지식을 기준으로 하여 소프트웨어 교육내용을 제시하여 보았다. 주요한 내용은 4개 영역을 모두 삽입하였고 교육내용과 교수법을 가장 강조하여 구성하고 소프트웨어교과교육 전문가에게 타당성 검토를 의뢰하였다. 전문가 설문 조사 결과 대부분 타당도와 합의도가 기준치를 넘었지만 학생관리 분야에 대해서 CVR값이 기준값을 하회한 것으로 나타났다.

• 한국언론정보학보
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• 제71권
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• pp.224-249
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• 2015

이 연구는 루만의 체계 이론을 통해, 2013년 12월에 발생한 '안녕들 하십니까?(이하 안녕들)' 현상을 분석했다. 루만에 따르면 커뮤니케이션은 타아에 의해 선택된 정보와 선택된 전달을 자아가 선택적으로 이해함으로써 시작하고 성립된다. 이때 이해는 내용의 수용과는 별개로, 그것은 후속 커뮤니케이션의 쟁점이다. 이처럼 선택을 토대로 한 커뮤니케이션의 설명은 안녕들 현상에 잘 적용되며, 아울러 커뮤니케이션에 대한 해석을 심화시킨다. 특히 안녕들 현상이 가져오는 후속 커뮤니케이션의 파생에 대한 해석을 위해 루만의 커뮤니케이션 이론은 적절하다. 안녕들 현상은 '안녕하지 못한 정치'에 대해 특정한 의미를 발생시키며 후속 커뮤니케이션을 지속적으로 유발하는 자기 생산적 체계다. 아울러 반박 대자보나 심지어 대자보 훼손 행위도 안녕들 현상이 발생시킨 '안녕하지 못한 정치'에 대한 부정을 야기하는데, 이 또한 커뮤니케이션의 중요한 요소다. 따라서 대자보는 안녕들 체계의 '안녕하지 않음'을 상징적으로 일반화한다고 볼 수 있다. 그래서 대자보 훼손은 안녕들 체계와는 근본적으로 구별되는 커뮤니케이션으로 관찰될 수 있다. 안녕들 체계는 대자보를 통해 사회에 더 나아간 커뮤니케이션을 시도하고 후속 커뮤니케이션을 기대한다.

• Journal of Information Processing Systems
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• 제14권3호
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• pp.751-770
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• 2018

Web applications are indispensable in the software industry and continuously evolve either meeting a newer criteria and/or including new functionalities. However, despite assuring quality via testing, what hinders a straightforward development is the presence of defects. Several factors contribute to defects and are often minimized at high expense in terms of man-hours. Thus, detection of fault proneness in early phases of software development is important. Therefore, a fault prediction model for identifying fault-prone classes in a web application is highly desired. In this work, we compare 14 machine learning techniques to analyse the relationship between object oriented metrics and fault prediction in web applications. The study is carried out using various releases of Apache Click and Apache Rave datasets. En-route to the predictive analysis, the input basis set for each release is first optimized using filter based correlation feature selection (CFS) method. It is found that the LCOM3, WMC, NPM and DAM metrics are the most significant predictors. The statistical analysis of these metrics also finds good conformity with the CFS evaluation and affirms the role of these metrics in the defect prediction of web applications. The overall predictive ability of different fault prediction models is first ranked using Friedman technique and then statistically compared using Nemenyi post-hoc analysis. The results not only upholds the predictive capability of machine learning models for faulty classes using web applications, but also finds that ensemble algorithms are most appropriate for defect prediction in Apache datasets. Further, we also derive a consensus between the metrics selected by the CFS technique and the statistical analysis of the datasets.

• 정보처리학회논문지:소프트웨어 및 데이터공학
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• 제2권4호
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• pp.251-266
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• 2013

정보화의 진전으로 행정 및 공공기관이 보유한 데이터에 대한 활용요구가 증대하고 있으나, 데이터 품질 문제로 많은 행정 및 공공기관이 공공 데이터의 개방 공유에 적극적으로 참여하고 있지 못하다. 본 연구에서는 선행연구에서 제시된 데이터 품질관리 성공요인을 조직적 차원과 관리적 차원으로 구분하고 이들 요인이 조직의 데이터 품질관리 수용을 통해 품질수준에 영향을 미치는지를 분석하였다. 연구결과, 품질관리 권장 및 지원과 같은 조직차원의 요인은 품질관리 수용에 영향을 미치며, 기관의 데이터 품질 수준과 관계를 가지는 것으로 나타났으나, 법제도 및 책임소재의 명확화와 같은 관리적 차원의 요인은 품질관리 수용에 영향을 미치지 않는 것으로 나타났다. 본 연구는 공공기관을 대상으로 한 품질관리가 초창기인 현 상황을 고려할 때 관리적 접근보다 전사차원의 공감대 형성이 필요함을 제시하였을 뿐만 아니라, 품질 수준에 직접적으로 영향을 미치는 품질수용 요인을 도출하여 제시하였다는데 의의를 가진다.

• 분석과 대안
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• 제1권2호
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• pp.13-30
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• 2017

Referred to as a 'media war,' there is a fierce competition for media discourse between different countries. Twenty four hour global news channels like Al Jazeera, France 24, RT, NHK World, China's CCTV and teleSUR emerged to offer their own perspectives and stance in the global society, and to face the monopolization and distorted information created by the hegemony of English news channels which have swayed international public opinions for a long time. As a tool of public diplomacy, the media's role in determining the image of the nation and winning the 'Hearts and Minds' of the international community is decisive, but it cannot be said that they all have a similar influence or play a positive role in media diplomacy. A global news channel, which is both a media diplomatic subject and a journalism organization, can be in the position of acting as a public relations organization or a propaganda agency for the government depending on the regime's attitude because most of global news channels receive support from the government. Sometimes it is difficult for these media to implement quality journalism because of financial difficulties. Media discourse also has limitations in that it is dependent upon changes in foreign policy of its own government. This study examines the current status of global news channels, the dilemma these channels are facing, and suggests some potential directions that can be taken by global news channels in order to become more effective. It is becoming increasingly important for all nations to respond to distorted information about their own countries, to appropriately identify various issues and changes in the international community and to convey their views and positions to the international community. For now, there is a lack of awareness about the importance of media diplomacy in Korea: There are many English-language media, but as yet no global news channel which could have an influence on the international stage. However, there seems to be some understanding about the need for the media to present the Korean alternative discourse to the senseless dependency on Western media. We hope that this study will be an opportunity to think in depth about the attitude of the Korean global media, whether existing global media or new global news channels, in order to help them become more effective in media diplomacy.