Genomics & Informatics

Korea Genome Organization (한국유전체학회)
권호 표지

Conservation of cis-Regulatory Element Controlling Timely Translation in the 3'-UTR of Selected Mammalian Maternal Transcripts

  • Lee, Hyun-Joo (Department of Bioengineering, Hankyong National University) ;
  • Lim, Yoon-Ki (Department of Bioengineering, Hankyong National University) ;
  • Chang, Sang-Ho (Department of Bioengineering, Hankyong National University) ;
  • Min, Kwan-Sik (Graduate School of Biotechnology, Environmental and Information Technology, Hankyong National University) ;
  • Han, Ching-Tack (Department of Life Science, Sogang University) ;
  • Hwang, Sue-Yun (Graduate School of Biotechnology, Environmental and Information Technology, Hankyong National University)
  • Published : 2007.12.31
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Abstract

The earliest stages of mammalian embryogenesis are governed by the activity of maternally inherited transcripts and proteins. Cytoplasmic polyadenylation of selected maternal mRNA has been reported to be a major control mechanism of delayed translation during preimplantation embryogenesis in mice. The presence of cis-elements required for cytoplasmic polyadenylation (e.g., CPE) can serve as a useful tag in the screening of maternal genes partaking in key functions in the transcriptionally dormant egg and early embryo. However, due to its relative simplicity, UA-rich sequences satisfying the canonical rule of known CPE consensus sequences are often found in the 3'-UTR of maternal transcripts that do not actually undergo cytoplasmic polyadenylation. In this study, we developed a method to confirm the validity of candidate CPE sequences in a given gene by a multiplex comparison of 3'-UTR sequences between mammalian homologs. We found that genes undergoing cytoplasmic polyadenylation tend to create a conserved block around the CPE, while CPE-like sequences in the 3'-UTR of genes lacking cytoplasmic polyadenylation do not exhibit such conservation between species. Through this cross-species comparison, we also identified an alternative CPE in the 3'-UTR of tissue-type plasminogen activator (tPA), which is more likely to serve as a functional element. We suggest that verification of CPEs based on sequence conservation can provide a convenient tool for mass screening of factors governing the earliest processes of mammalian embryogenesis.

Keywords

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