• 한국동물생명공학회지
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• 제36권2호
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• pp.91-98
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• 2021

The objective of this study was to establish a selection process for high quality sperm in bovine semen using sperm separation by magnetic activation (MACS). For this, semen from 21 Nellore bulls was collected using an artificial vagina. To guarantee the presence of pathologies in the ejaculate, animals previously declassified in four consecutive spermiogram were used. Semen was analyzed in five statuses: (1) fresh semen (fresh); (2) density gradient centrifugation (DGC), percoll column; (3) non-apoptotic fraction after separation by MACS (MAC); (4) apoptotic fraction from the separation (MACPOOR); and (5) MAC followed by DGC (MACDGC). Using a computerized analysis system (CASA), motility was measured. The sperm morphology was evaluated by phase contrast, and the supravital test was completed with eosin/nigrosin staining. For DGC, 20 × 10⁶ cells were used in a gradient of 90% and 45% percoll. MACS used 10 × 10⁶ cells with 20 μL of nanoparticles attached to annexin V, and filtered through the MiniMACS magnetic separation column. Membrane integrity was assessed with SYBR-14/IP and mitochondrial potential with JC-1 by flow cytometry. Processing sperm by MACDGC, was more effective in obtaining samples with high quality sperm, verified by the total of abnormalities in the samples: 35.04 ± 2.29%, 21.50 ± 1.47%, 17.30 ± 1.10%, 30.68 ± 1.94% and 10.50 ± 1.46%, respectively for fresh, DGC, MAC, MACPOOR, and MACDGC. The subpopulation of non-apoptotic sperm had a high number of live cells (82.65%), membrane integrity (56.60%) and mitochondrial potential (83.98%) (p < 0.05). These findings suggest that this nanotechnological method, that uses nanoparticles, is efficient in the production of high-quality semen samples for assisted reproduction procedures in cattle.

• 한국의류학회지
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• 제45권2호
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• pp.233-252
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• 2021

The Gwan ceremony (冠禮) is a coming-of-age ceremony that takes place through traditional Gwan (冠: the hat) and clothes in the traditional era. The rite is performed by wearing hats and clothes three consecutive times (三加禮). It was an important rite which meant that underage children were formally recognized as members of society. This study examined costumes of people who participated in various coming-of-age ceremony rites in the Ming dynasty imperial court of China. For the research data, this study mainly used authentic chronicles (正史), codes of law and books on Ming dynasty rituals. This study examined the costumes used in the coming-of-age ceremony for the emperor, Prince Imperial, Emperor's eldest grandson, and emperor's sons. The results of this study were divided into an analysis of the document structure, institutional changes by time, characteristics of costumes, and characteristics of the rite. Of particular note in their ceremony, the emperor is presupposed to be a 'human already full-equipped with virtue', which means that the costume is worn only once. It is a case in which the emperor's absolute identity is revealed through the rite and costume.

• 건축역사연구
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• 제31권6호
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• pp.69-80
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• 2022

This study explains details on Heungbokjeon during the reconstruction of Gyeongbokgung Palace in the 19th century for the first time in 273 years. Hence, the construction of a palace with more than 7,000 Kan(間) made possible in a short stretch of time. Given the fact that its existing architecture was recycled. The antecedent of Heungbokjeon(興福殿) was the Hamiljae(咸一齋) of Changuiggung(彰義宮) which served as the residence of King Yeongjo before he was enthroned. On the other hand, the Changuigung was the outset of Inpyeongwi-gung(寅平尉宮), which was built for the princesses. During the reign of King Hyojong the princess who got married must depart from the Inpyeongwi-gung. This study corroborated the undertaking of modification of its architecture frombeing asymmetrical house to symmetrical most primarily the inner part of the palace through the innovation of Changui-gung and the restoration of Hamijae to Heungbokjeon. Hence, it was confirmed that the Heungbokjeon was the only inner part of the palace that was not burned down by the 2 consecutive fires during the reign of King Gojong, to be more specific these fire happend on his 10th and 13th year of rule. Consequently, the research process of Gyeongbokgung Heungbokjeon can escalate the possibility of considering not only the reconstruction of Gyeongbokgung in the 19th century, as well as the construction of the capital city in the 17th century.

• International Journal of Industrial Entomology and Biomaterials
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• 제47권1호
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• pp.34-43
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• 2023

The eighteen (18) F₁ hybrid combinations were tested to identify potential combinations adaptable to type 1 climatic conditions in the Philippines. The six (6) bivoltine purelines (DMMMSU 108, DMMMSU 109, DMMMSU 110, DMMMSU 111, DMMMSU 113, and DMMMSU 119); and three (3) multivoltine purelines (DMMMSU 1000, DMMMSU 1007, and DMMMSU 1014), were crossed (multivoltine x bivoltine) in a mating plan. These were arranged in a Completely Randomized Design (CRD), replicated three times, and analyzed using Analysis of Variance (ANOVA). A test of significance was done using ANOVA across years and Tukey's Honest Significant Difference Test (HSD). The multiple trait evaluation index (EI) method was also used in the identification of potential F₁ hybrids. Three major phases were done: (1) parental rearing of multivoltine and bivoltine pure lines for breed multiplication; (2) hybridization process; and (3) evaluation of F₁ hybrids. Rearing evaluations were conducted for three consecutive years. Based from the three evaluations, 10 potential crosses were identified: DMMMSU MV-12, DMMMSU MV-11, DMMMSU MV-13, DMMMSU MV-16, DMMMSU MV-07, DMMMSU MV-14, DMMMSU MV-05, DMMMSU MV-09, DMMMSU MV-03, and DMMSU MV-10. The topmost combinations with the best economic and commercial characters and are consistently adaptable during two (2) cropping seasons were DMMMSU MV-07, DMMMSU MV-12, DMMMSU MV-05, DMMMSU MV-09 and DMMMSU MV-11. These newly-identified F₁ hybrids are considered potential breeds that could improve cocoon production.

• 한국컴퓨터정보학회논문지
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• 제14권10호
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• pp.225-231
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• 2009

본 논문에서는 시간이 변하는 볼륨 데이터로부터 등위면 콤포넌트를 계산하고 그 움직임을 추적하는 새로운 시각화 알고리즘을 기술한다. 등위면 시각화는 볼륨 데이터를 효과적으로 보여주기 위한 가장 흔한 방법 중 하나이다. 그러나 대부분의 기존 등위면 시각화 알고리즘들은 시간이 정지되어 있는 정적 볼륨 데이터에 국한하여 적용되어왔다. 이미징 기술과 시뮬레이션 기술이 발전함에 따라 대용량의 시변 볼륨 데이터들이 생성되고 있어 이러한 시변볼륨 데이터의 동적인 특성을 이용할 수 있는 효과적인 시변 등위면 시각화 알고리즘이 필요하게 되었다. 우리는 우선 두 연속되는 시변 볼륨 데이터에서 정의되는 등위면 컴포넌트들간의 시간적 일치성을 정의한다. 이 정의에 기반하여 컨투어 트리를 이용하여 얻어진 등위면 컴포넌트로부터 그것과 일치되는 그 다음 시간단계에서의 컴포넌트들을 추적하는 알고리즘을 수행한다. 이러한 과정을 매 시간단계에서 반복하면, 선택된 등위면 컴포넌트의 동적인 변형 모습을 보여주어 효과적인 시각화를 이룰수 있다.

• 한국정보통신학회논문지
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• 제26권12호
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• pp.1926-1933
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• 2022

변수내장형 다채널 위너 필터는 내장된 변수를 이용하여 잔여잡음과 신호왜곡 간의 트레이드오프를 조절할 수 있는 선형 필터이다. 이러한 변수내장형 다채널 위너필터를 적용하기 위해서는 정확한 잡음추정이 중요한데 널리 쓰이는 다채널 최소 제어 재귀 평균 기법이 있다. 하지만 다채널 최소 제어 재귀 평균 기법은 방향성 간섭 신호가 존재할 경우 잡음추정의 정확도가 하락하여 변수내장형 다채널 위너필터의 성능이 저하되는 문제점이 있다. 따라서, 본 논문에서는 변수내장형 다채널 위너필터를 위한 새로운 잡음 추정 기법을 제안한다. 제안한 방법은 주로 잡음 섞인 마이크로폰 입력 신호의 전력 스펙트럼 밀도에 대해 고유값 분해, 방향성 정보를 이용한 목적신호의 기여도 추정, 목적신호의 기여도를 보다 합리적으로 추정하기 위한 지수 가중치 부가의 일련의 과정을 수행한다. 제안한 방법을 평가하기 위해 신호대잡음비, 음성왜곡도 등의 총 4가지 객관적 성능 평가 방법을 이용하여 기존의 방법과 비교하였다. 실험을 통해 방향성 간섭신호가 존재하는 환경에서 제안한 잡음 추정기법을 적용한 다채널 위너필터의 성능이 향상됨을 확인하였다.

• 운동영양학회지
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• 제23권3호
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• pp.13-21
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• 2019

[Purpose] Chronic stress is a precipitating factor for depression, whereas exercise is beneficial for both the mood and cognitive process. The current study demonstrates the anti-depressive effects of regular exercise and the mechanisms linked to hippocampal neurogenesis. [Methods] Mice were subjected to 14 consecutive days of restraint, followed by 3 weeks of treadmill running, and were then subjected to behavioral tests that included the forced swimming and Y-maze tests. Protein levels were assessed using western blot analysis and newborn cells were detected using 5-bromo-2'-deoxyuridine (BrdU). [Results] Three weeks of treadmill running ameliorated the behavioral depression caused by 14 days of continuous restraint stress. The exercise regimen enhanced BrdU-labeled cells and class III β-tubulin levels in the hippocampal dentate gyrus, as well as those of thioredoxin-1 (TRX-1) and synaptosomal β2-adrenergic receptors (β2-AR) under stress. In vitro experiments involving treatment with recombinant human TRX-1 (rhTRX-1) augmented the levels of phospho-extracellular signal-regulated kinases 1 and 2 (ERK1/2), nuclear β-catenin, and proliferating cell nuclear antigens, which were previously inhibited by U0216 and FH535 (inhibitors of ERK1/2 and β-catenin/T cell factor-mediated transcription, respectively). The hippocampal neurogenesis elicited by a 7-day exercise regimen was abolished by a selective inhibitor of β2-AR, butoxamine. [Conclusion] These results suggest that TRX-1-mediated hippocampal neurogenesis by β2-AR function is a potential mechanism underlying the psychotropic effect of exercise.

• 대한조선학회논문집
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• 제61권3호
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• pp.143-151
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• 2024

In this study, we present a mapping framework for 3D spatial reconstruction of digital twin model using navigation and perception sensors mounted on an Autonomous Surface Vehicle (ASV). For improving the level of realism of digital twin models, 3D spatial information should be reconstructed as a digitalized spatial model and integrated with the components and system models of the ASV. In particular, for the 3D spatial reconstruction, color and 3D point cloud data which acquired from a camera and a LiDAR sensors corresponding to the navigation information at the specific time are required to map without minimizing the noise. To ensure clear and accurate reconstruction of the acquired data in the proposed mapping framework, a image preprocessing was designed to enhance the brightness of low-light images, and a preprocessing for 3D point cloud data was included to filter out unnecessary data. Subsequently, a point matching process between consecutive 3D point cloud data was conducted using the Generalized Iterative Closest Point (G-ICP) approach, and the color information was mapped with the matched 3D point cloud data. The feasibility of the proposed mapping framework was validated through a field data set acquired from field experiments in a inland water environment, and its results were described.

• Clinical Endoscopy
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• 제55권4호
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• pp.558-563
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• 2022

Background/Aims: Bispectral index (BIS) monitors process and display electroencephalographic data are used to assess the depth of anesthesia. This study retrospectively evaluated the usefulness of BIS monitoring during endoscopic ultrasonography (EUS). Methods: This study included 725 consecutive patients who underwent EUS under sedation with propofol. BIS monitoring was used in 364 patients and was not used in 361. The following parameters were evaluated: (1) median dose of propofol; (2) respiratory and circulatory depression; (3) occurrence of body movements; (4) awakening score >8 at the time; and (5) awakening score 2 hours after leaving the endoscopy room. Results: The BIS group received a significantly lower median dose of propofol than the non-BIS group (159.2 mg vs. 167.5 mg; p=0.015) in all age groups. For patients aged ≥75 years, the reduction in heart rate was significantly lower in the BIS group than in the non-BIS group (1.2% vs. 9.1%; p=0.023). Moreover, the occurrence of body movements was markedly lower in the BIS group than in the non-BIS group (8.5% vs. 39.4%; p<0.001). Conclusions: During EUS examination, BIS monitoring is useful for maintaining a constant depth of anesthesia, especially in patients 75 years of age or older.

• 대한두개안면성형외과학회지
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• 제25권4호
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• pp.161-170
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• 2024

Background: The eyes are the central aesthetic unit of the face. Maxillofacial trauma can alter facial proportions and affect visual function with varying degrees of severity. Conventional approaches to reconstruction have numerous limitations, making the process challenging. The primary objective of this study was to evaluate the application of three-dimensional (3D) navigation in complex unilateral orbital reconstruction. Methods: A prospective cohort study was conducted over 19 months (January 2020 to July 2021), with consecutive enrollment of 12 patients who met the inclusion criteria. Each patient was followed for a minimum period of 6 months. The principal investigator carried out a comparative analysis of several factors, including fracture morphology, orbital volume, globe projection, diplopia, facial morphic changes, lid retraction, and infraorbital nerve hypoesthesia. Results: Nine patients had impure orbital fractures, while the remainder had pure fractures. The median orbital volume on the normal side (30.12 cm³; interquartile range [IQR], 28.45-30.64) was comparable to that of the reconstructed orbit (29.67 cm³; IQR, 27.92-31.52). Diplopia improved significantly (T(10)= 2.667, p= 0.02), although there was no statistically significant improvement in globe projection. Gross symmetry of facial landmarks was achieved, with comparable facial width-to-height ratio and palpebral fissure lengths. Two patients reported infraorbital hypoesthesia at presentation, which persisted at the 6-month follow-up. Additionally, five patients developed lower lid retraction (1-2 mm), and one experienced implant impingement at the infraorbital border. Conclusion: Our study provides level II evidence supporting the use of 3D navigation to improve surgical outcomes in complex orbital reconstruction.