• Asian-Australasian Journal of Animal Sciences
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• 제25권5호
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• pp.648-652
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• 2012

This study was conducted to determine the effects of dietary addition of tea saponins (TS) and soybean oil (SO) on fatty acid profile and gene expression of stearoyl-CoA desaturase (SCD) in longissimus dorsi (LD) muscle of growing lambs. Thirty-two Huzhou lambs were assigned to four dietary treatments in a $2{\times}2$ factorial arrangement with main effects of TS (0 or 3 g/d) and SO (0 or 30 g/kg of diet DM). The diet without additives was considered as NTNS (no TS or SO). After a feeding trial for 60 d, four lambs of each treatment were slaughtered to collect the samples of LD muscle. Percentage of trans-11 vaccenic acid was enhanced (p<0.05) in muscle of lambs fed TS and SO. The proportion of total conjugated linoleic acid (CLA) was increased (p<0.05) by SO, but decreased (p<0.05) by TS in LD muscle. The percentage of total saturated fatty acids in muscle was decreased (p<0.05) by addition of TS and SO, while addition of SO increased (p<0.05) the percentage of total polyunsaturated fatty acids. The ratio of cis-9, trans-11 CLA to tran-11 vaccenic acid was decreased (p<0.05) by TS, but increased (p<0.05) by SO. The same effects were observed in SCD mRNA expression. From these results it is indicated that including TS and SO in the diet of growing lambs affect the fatty acid profiles of LD muscle and that the proportion of cis-9, trans-11 CLA in the muscle influenced by TS and SO may be related to the SCD gene expression.

• 한국식품위생안전성학회지
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• 제22권3호
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• pp.145-150
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• 2007

Conjugated linoleic acid (CLA)는 전지방세포의 증식 및 분화를 억제시키므로서 포유동물에서 지방의 축적을 감소시키는 것으로 알려져 있다. 논 연구의 목적은 diglyceride(DG), CLA, 및 DG-CLA가 전지방세포의 증식 및 분화를 억제시키는지를 알아보고자, 세포생존측정, glycerol-3-phosphate dehydrogenase (GPDH) 활성측정, 지방축적에 대한 Oil red O 염색을 실시하였다. DG, CLA, 및 DG-CLA의 처치는 전지방세포의 증식을 농도 의존적으로 감소시켰으며, 그러한 효과는 CLA에서 가장 강하게 나타났다. 이들 시험물질의 $100 {\mu}g/ml$ 농도에서 DG, CLA, DG-CLA는 유의적으로 GPDH의 활성을 낮추었으며(p<0.05), 이러한 결과는 전지방세포의 분화를 억제하는 것을 의미한다. 더불어 이들 시험물질은 전지방세포의 분화과정 중에 지방축적을 효과적으로 억제시켰다. 더욱이 DG-CLA가 DG 혹은 CLA보다 분화 및 지방축적에 대해 더 강한 효과를 보였다. 이러한 결과로부터 DG-CLA의 섭취는 사람에서 체중조절의 유용한 효과를 얻을 수 있을 것이다.

• 한국식생활문화학회지
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• 제18권1호
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• pp.1-8
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• 2003

Recently, the concern about safety and functional substances of foods are increased therefore antioxidant substances and plants which had pharmacological effect have been studied actively. It has been known that the mulberry leaf is effective in curing many diseases. Nowaday, the pharmachological effects of mulberry leaves on diabetes mellitus and their usage for many aspects were confirmed. Mulberry leaves are known for effective in prevention of diabetes mellitus, because of specific amino acids and fibers. In this study, methanol, hexane, chlorform, ethyl acetate, and butanol extracts obtained from mulberry leaves were added to soybean oil and they were stored for 30 days at $60{\pm}2^{\circ}C$ and peroxide value(POV) and conjugated diene value(CDV) were measured periodically. Results of this study were obtained as follows; 1. The POV of soybean oil after the addition of each mulberry leaves powder(MLP) extracts generally enhanced as the storage time was prolonged, so the POV of all samples was reached higher than 100meq./kg.oil after 10 days storage without the addition of butanol, methanol, ethylacetate, hexane extracts at 0.1% level. Especially, the POV of soybean oils including butanol extract was 87.35meq/kg.oil after 10 days storage and antioxidant activity of butanol extract was shown to be superior to that of BHT. The pattern of the changes of the CDV of soybean oil after the addition of MLP extracts at 0.02%, 0.05% and 0.1%, respectively, were almost constant during 10 days of storage and then rapidly increased during the rest of experimental periods. During 10 days of storage in case of 0.1% adding level, the antioxidant activities of the butanol extract was superior to that of the each MLP extracts.

• 한국가금학회지
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• 제38권3호
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• pp.197-204
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• 2011

본 연구는 기능성 물질인 누에고치 분말, 동충하초 분말과 CLA의 단독 혹은 혼합 첨가가 산란 노계 가슴살에서 회수한 단백질을 이용하여 명태 연육을 20% 대체한 크래미의 저장 중 이화학적 및 조직학적 특성을 분석한 결과이다. pH 및 백색도를 나타내는 W는 누에고치 및 동충하초 분말을 함께 첨가한 T2에서 유의적으로 높게 나타난 반면, 전단력은 T2가 가장 낮았다(p<0.05). 명도를 나타내는 $L^*$과 탄력성에서는 누에고치 분말을 첨가한 T1이 가장 높았으며, 적색도를 나타내는 $a^*$, 황색도를 나타내는 $b^*$ 및 파괴 강도에서는 T1이 다른 처리구에 비해 유의적으로 낮았다(p<0.05). 표면경도와 부착성에서는 동충하초 분말과 CLA를 함께 첨가한 T3에서 유의적으로 높게 나타났으며, 파괴 강도 및 겔 강도에서는 T2와 T3가 높았다(p<0.05). 따라서 본 실험은 산란 노계 가슴살 회수단백질을 이용하여 명태 연육을 대체하며 제조한 크래미에 누에고치 분말과 동충하초 분말을 함께 첨가하며 생산한다면 크래미의 품질 특성 평가에 있어 중요한 항목으로 구분되는 백색도와 겔 강도를 향상시키고, 전단력을 감소시켜 안정적인 조직과 높은 연도를 가진 제품의 생산이 가능할 것으로 판단된다. 이와 더불어 누에고치와 동충하초가 가진 항암과 항산화 효과 및 뇌 기능의 활성화 등 다양한 생리 활성 효과를 함유한 기능성 크래미를 제조 판매 할 수 있어 소비자의 구매 욕구를 충족시킬 수 있을 것으로 판단된다.

• Applied Biological Chemistry
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• 제40권4호
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• pp.283-288
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• 1997

1% linoleic acid를 함유하는 수용성 완충용액과 LDL(low-density lipoprotein, 1 mg protein/ml)을 함유하는 수용성 완충용액 각각에 $H_2O_2$와 $FeCl_2$를 가하여 유발된 산화과정 중 한국산 홍삼 extract(red ginseng extract; RGE)에 대한 산화방지활성을 비교물질로 ${\alpha}-tocopherol$을 사용하여, HPLC를 이용한 MDA(malondialdehyde) 정량법과 fluorometry를 이용하여 측정 비교하였다. LDL(0.25 mg protein/ml)에서 생성된 conjugated diene도 spectrometry로 측정하였다. Linoleic acid를 기질로 사용한 경우, MDA 생성량으로 비교한 산화방지효과에 있어서 1000 ppm RGE의 첨가로 71.8%의 우수한 산화저해율이 나타났으며, 이때 비교물질로 사용한 100 ppm ${\alpha}-tocopherol$의 경우에는 76.1%이었다. LDL(1 mg/ml)을 기질로 사용하였을 경우에는 RGE 200 ppm 첨가시 가장 우수한 항산화효과가 나타났으며, 이때의 산화저해율은 25.2%이었고, 100 ppm ${\alpha}-tocopherol$의 경우는 21.2%이었다. 또한 LDL(0.25 mg protein/ml)을 기질로 사용하여 생성된 c-diene을 측정한 경우에도 50 ppm RGE의 첨가로 44.2% 저해효과가 입증되었다.

• 한국임상수의학회지
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• 제33권4호
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• pp.194-199
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• 2016

The objective of this study was to examine the effect of trans-10, cis-12 conjugated linoleic acid (t10c12-CLA) on the expression of cyclooxygenase-2 (COX-2) and 5-lipoxygenase (5-LOX) pathway in lipopolysaccharide (LPS)-stimulated porcine peripheral blood mononuclear cells (PBMCs). t10c12-CLA was treated with different concentrations in culture medium of LPS$na{\ddot{i}}ve$ and LPS-stimulated PBMCs. The mRNA expressions of prostaglandin $E_2$ ($PGE_2$)-synthase, COX-2 and 5-LOX were measured using quantitative real-time PCR. In addition, the production levels of $PGE_2$ and 5-LOX in culture supernatant from PBMCs with or without LPS were assessed by ELISA. In LPS$na{\ddot{i}}ve$ PBMCs, treatment of t10c12-CLA significantly (p < 0.05) increased the mRNA expressions of PGE2 synthase and 5-LOX compared to vehicle control. Expression of COX-2 mRNA did not show significant difference compared to vehicle control by t10c12-CLA treatment in LPS$na{\ddot{i}}ve$ PBMCs. However, the addition of LPS in PBMCs markedly (p < 0.05) increased the mRNA expression of COX-2, $PGE_2$ synthase and 5-LOX, and also significantly (p < 0.05) enhanced the production of $PGE_2$ and 5-LOX relative to LPS$na{\ddot{i}}ve$ PBMCs, respectively. However, the addition of t10c12-CLA significantly (p < 0.01) suppressed the LPS-induced excessive expression of COX-2, $PGE_2$ synthase, and 5-LOX compared to those of PBMCs treated with LPS alone. The production levels of $PGE_2$ and 5-LOX in culture supernatant from LPS-stimulated PBMCs were also significantly (p < 0.05) inhibited by the treatment of t10c12-CLA compared to LPS alone. These results suggested that t10c12-CLA has an anti-inflammatory effect via dual inhibition of COX-2 and 5-LOX with gene expression and production level in LPS-stimulated porcine PBMCs. Therefore, it was thought that t10c12-CLA can attenuate the inflammatory response by down-regulation of eicosanoids production.

• Biotechnology and Bioprocess Engineering:BBE
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• 제10권4호
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• pp.357-361
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• 2005

The bioconversion of linoleic acid (LA) to conjugated linoleic acid (CLA) was investigated to examine LA-adaptation of Bifidobacterium breve KCTC 3461 to additions of 1 to 5 mg/mL of LA overtime. To induce LA-adaptation, B. breve KCTC 3461 was treated with LA, according to three schemes. For LA-adapted B. breve the maximum concentration of CLA, $300\~350{\mu}g/mL$, was obtained in cys-MRS medium containing 1 mg/mL of LA. The CLA production significantly increased with increasing LA concentration, from 1 to 4 mg/mL, but the conversion of LA to CLA gradually decreased. The CLA production capability of B. breve, and its tolerance, improved significantly with LA-adaptation. The addition of LA (1 mg/mL) into the culture broth after 24 h of cultivation in a 100-mL media bottle was most effective at promoting CLA production. In a 2.5-L stirred-tank bioreactor, the observed conversion and productivity of $56.6\%\;and\;35.4{\mu}gmL^{-1}h^{-1}$, respectively, by LA-adapted B. breve were approximately 6.6 and 9.8 times higher than those of LA-unadapted B. breve.

• Preventive Nutrition and Food Science
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• 제6권1호
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• pp.57-61
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• 2001

Conjugated linoleic acid (CLA), when incorporated into mouse liver microsomal membranes, selectively inhibits the mutagenesis of 2-amino-3-methylimidazo[4,5-f] quinoline (IQ). Nine-week old female ICR mice were given (p.o.) 0.1 mL olive oil alone (control), 0.1 mL olive oil plus 0.1 mL linoleic acid, or 0.1 mL olive oil plus 0.1 mL CLA, twice weekly for four weeks. The animals were then sacrificed and liver S-9 fractions were prepared. Activation of IQ for mutagenesis by the liver S-9 from CLA-treated mice was significantly reduced in comparison wit liver S-9 from control or linolic acid-treated mice. By contrast, the activation of 7,12-dimethylbenz[a] anthracene (DMBA) and benzo[a] pyrene (BP) was unaffected. Hence, CLA incorporated into phospholipids may selectively affect cytochrome P450 isozymes responsible for activating IQ, but not those which activate BP or DMBA. The addition of free CLA or the methyl esters of CLA, linoleic acid, or oleic acid, to control S-9 inhibited the activation of all three mutagens (IQ, BP, and DMBA).

• Korean Chemical Engineering Research
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• 제52권2호
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• pp.205-208
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• 2014

본 논문에서는 유기전자소자의 반도체 물질로 많이 사용되는 poly(9,9-dioctylflurorene) (PFO), poly(9,9-dioctylfluorene-co-benthiadiazole) (F8BT), (regioregular poly(3-hexylthiophene) (P3HT)를 기반으로 하는 균일한 크기와 특성을 가지는 고분자 나노와이어를 AAO 템플레이트를 이용하여 대량으로 제작하였다. 제작된 나노와이어는 결점이 없이 깨끗한 표면을 보였으며, 약 250~300 nm의 지름과 ${\sim}30{\mu}m$의 일정한 길이를 가지고 있었다. 나노와이어들은 스프레이 분사 방법을 통하여 유리 기판 위에 균일하게 분사할 수 있었으며, PFO와 F8BT 나노와이어의 경우 UV 빛의 조사하에 각각 나노와이어의 전체에 걸쳐서 왜곡없이 밝은 yellow와 blue luminescence를 보였다.