• Journal of Plant Biotechnology
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• 제37권4호
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• pp.343-356
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• 2010

Plant micropropagation techniques include bud cultures using apical or axillary buds, organogenesis through callus culture or adventitious bud induction, and somatic embryogenesis. In Korea Forest Research Institute (KFRI), the first tissue culture trial in woody plant was initiated from the bud culture of hybrid poplars (Populus alba x P. glandulosa) in 1978. Since then several mass propagation techniques have developed from conifer and hardwood species, resulting in allowing practical application to Poplars, Birches and some oak species. In addition, useful micropropagation and genetic resources conservation techniques were established in some rare and endangered tree species including Abeliophyllum distichum. Among various in vitro propagation techniques, somatic embryogenesis is known to be the most efficient plant regeneration system. Since the first somatic embryo induction was reported in Tilia amurensis by KFRI in 1986, various protocols for direct or indirect somatic embryogenesis systems have developed in conifer and hardwood species including Larix leptolepis, Pinus rigida x P. taeda F1, Kalopanax septemlobus and Liliodendron tulipifera, etc. However, most of these technologies have been developed using juvenile tissues, i.e. immature zygotic embryos or mature embryos. Therefore it has been difficult to directly application to tree breeding program due to their unproven genetic background. Recently remarkable progresses and new approaches have been achieved in mature tree somatic embryogenesis. In this article we reviewed several micropropagation techniques, which have been mainly developed by KFRI and recent international progresses.

• 한국기후변화학회지
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• 제8권4호
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• pp.401-414
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• 2017

Vegetation and landscape characteristics at the three highest summits of Republic of Korea, i.e. Seoraksan, Jirisan and Hallasan, are analyzed on the basis of species composition, physiognomy, vegetation distribution and structure of alpine plants, along with landform, geology, soil and habitat conditions. Dominant high mountain plants at three alpine and subalpine belts contain deciduous broadleaved shrub, Rhododendron mucronulatum var. ciliatum (31.6%), and evergreen coniferous small tree, Pinus pumila (26.3%) at Seoraksan, deciduous broadleaved tree, Betula ermanii (35.3%), evergreen coniferous tree, Picea jezoensis (23.5%) at Jirisan, and evergreen coniferous tree, Abies koreana (22.6%), deciduous broadleaved shrub, Rhododendron mucronulatum var. ciliatum, and Juniperus chinensis var. sargentii (19.4%) at Hallasan, respectively. Presence of diverse landscapes at the peak of Seoraksan, such as shrubland, grassland, dry land along with rocky areas, and open land may be the result of hostile local climate and geology. High proportion of grassland and wetland at the top of Jirisan may related to gneiss-based gentle topography and well developed soil deposits, which are beneficial to keep the moisture content high. Occurrence of grassland, shrubland, dry land, conifer vegetation, and rocky area at the summit of Hallasan may due to higher elevation, unique local climate, as well as volcanic origin geology and soil substrates. Presences of diverse boreal plant species with various physiognomy at alpine and subalpine belts, and wide range of landscapes, including rocky, grassland, shrubland, wetland, and conifer woodland, provide decisive clues to understand the natural history of Korea, and can be employed as an relevant environmental indicator of biodiversity and ecosystem stability.

• 한국약용작물학회지
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• 제6권1호
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• pp.21-27
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• 1998

본(本) 실험(實驗)은 자생지(自生地)의 야생(野生)더덕이 가지는 고방향성(高芳香性)의 특성(特性)을 노지재배시(露地栽培時) 재배(栽培)더덕에서도 고방향성(高芳香性)을 갖는 재배기술(栽培技術)을 확립(確立)하기 위하여 몇가지 유기물(有機物)을 시용(施用)하여 더덕의 주요성분(主要成分), 유리(遊離)아미노산(酸) 및 식물정유(植物精油), 향기성분(香氣成分)의 변화(變化)를 검토(檢討)한 결과(結果)는 다음과 같다. 1. 위장(萎長)은 자생지토양(自生地土壤)보다 볏짚퇴비(堆肥)와 활엽수퇴비(闊葉樹堆肥) 시용구(施用區)에서 가장 길었고 엽장(葉長), 엽폭(葉幅) 등(等)은 침엽수(針葉樹)/이끼 堆肥(퇴비) 시용구(施用區)에서 가장 컸다. 2. 조단백질(粗蛋白質) 함량(含量)은 자생지(自生地) 토양(土壤)보다 침엽수(針葉樹) 이끼퇴비(堆肥)와 계분(鷄糞)톱밥 퇴비(堆肥) 시용구(施用區)에서는 낮은 반면에 산야초(山野草) 퇴비(堆肥), 활엽수(闊葉樹) 퇴비(堆肥) 및 볏집 퇴비(堆肥)에서는 높은 함량(含量)을 나타내었고 무기성분(無機成分)인 K와 Mg은 전반적으 볏짚 퇴비(堆肥)와 침엽수(針葉樹) 이끼퇴비(堆肥) 시용구(施用區)에서 높은 함량(含量)을 보였으나, Fe, Mn, Zn, Cu 등(等) 미량원소(微量元素)는 유기물(有機物) 종류간(種類間)에 일정(一定)한 경향(傾向)이 없었다. 3. 유리(遊離) 아미노산(酸) 함량(含量)에서 arginine은 다른 유리(遊離) 아미노산(酸)보다 매우 높았고 활엽수(開葉樹) 퇴비(堆肥) 시용구(施用區)에서 18.99mg으로 가장 높은 함량(含量)을 보였다. 4. 유기물(有機物) 종류(種類)에 따른 식물정유(植物精油) 함량(含量)은 침엽수(針葉樹)/이끼 퇴비(堆肥) 시용구(施用區)에서 는 0.008%로 가장 높은 수율(收率)을 나타내어 자생지(自生地) 유기물(有機物) 자원(資源)과 가장 유사(類似)한 것으로 판단(判斷) 되었다. 5. 더덕 뿌리의 향기성분(香氣成分)을 GC/MS에 의해 분석(分析)한 결과(結果) 총 48종이 확인(確認)되었고 1-hexanol, cis-3-hexenol, trans-2-hexanol 등(等)이 높은 함양(含量)을 보였다. 유기물(有機物) 종류간(種類間)에는 침엽수(針葉樹)/이끼 퇴비(堆肥)에서 주요(主要) 향기성분(香氣成分)인 1-hexanol이 78.62, cis-3-hexenol이 36.65. trans-2-hexanol이 120.41 peak area%를 나타내었으며 이는 향기(香氣)가 높은 자생지(自生地) 토양(土壤)의 1-hexanol이 74.47, cis-3-hexenol이 44.21, trans-2-hexanol이 147.33 peak area%인 것과 유사(類似)하게 높았다.

• 한국수자원학회:학술대회논문집
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• 한국수자원학회 2009년도 학술발표회 초록집
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• pp.635-640
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• 2009

본 연구에서는 침엽수 산림에서 토양수분 변화를 모니터링하고 이를 분석하기 위하여 서울대학교 농업생명과학대학 태화산 학술림의 잣나무 조림지에 FDR (Frequency Domain Reflection) 토양수분측정장치와 지온센서 등을 설치하였다. 이와 같이 설치한 센서를 이용하여 2008년 4월부터 9월까지 토양수분 자료를 수집하였으며, 경기도 광주시 모현면의 AWS의 기상자료를 이용하여 증발산량, 강우량과 토양수분의 연관성을 분석하였다. 토양수분 모니터링 자료를 활용하여 산림에서의 깊이별, 계절별 토양수분 소비 패턴을 분석하였으며, 토양수분 모니터링 자료 및 강수량 자료를 이용하여 물수지법에 의한 증발산량을 산정하여 제시하였다.

• 한국실내디자인학회논문집
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• 제17호
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• pp.157-165
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• 1998

This study aimed to identify characteristics of design and decoration of Danish peasant furniture especially painted storage furniture used during the 18-19th century before modern design movement of Internationalism prevailed all over the world. Owing to the fact that Danish peasant furniture were made of available conifer around the farms painting method was preferred to carving. Remoted from main land of western and southern Europe Scandinavian countries longed to imitate their Baroque style architecture and furniture made of marble of hard woods. Painting method was adopted to disguise cheap wood so that it looked like expensive or exotic materials such as marble oak metal granite etc. what they could not afford to buy. Eventually they evolved unique materials equipments and methods for imitation painting in order to decorate folk storage furnitures as well as formal architecture : palaces churches and other official buildings,. Marbling clouds marbling graining stencil spatter painting trompe-I'oeil were common to imitate stones or hard woods. Strong and bright colors had good combination together with dull colors on the Danish peasant painted furniture.

• 대한원격탐사학회:학술대회논문집
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• 대한원격탐사학회 2006년도 춘계학술대회 논문집
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• pp.267-270
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• 2006

본 논문은 국산 고해상도 위성화상의 활용 일환으로 산불피해림의 정밀정성정보를 제공하기 위한 낙엽송 및 리기다 소나무의 산불피해등급 육안판독열쇠에 관한 연구이다. 본 육안 판독열쇠는 낙엽송과 리기다 소나무의 산불피해등급 판별을 위해 4가지 요소, 즉 색, 형태, 구조 그리고 질감에 기초로 개발하였다.

• 대한원격탐사학회:학술대회논문집
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• 대한원격탐사학회 2006년도 춘계학술대회 논문집
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• pp.210-213
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• 2006

이 연구에서는 다중시기 Landsat 영상을 이용하여 강원도 일대 임상의 변화를 살펴보고 상록수의 영급을 구분하는 알고리즘을 개발하여 적용하였다. 1980년대에서 현재까지 축적된 Landsat-5와 Landsat-7영상 중에서, 대부분 지역에 활잡목 및 활엽수가 낙엽이 지고 눈이 아직 쌓이지 않을 시기인 11월에 촬영된 영상만을 이용하였다. 각 영상에서 양지바른 상록수, 활엽수, 그늘진 지역, 도시 및 바다 등을 클래스로 지정하여 감돌분류를 하였다. 분류 결과에서 양지바른 상록수만 추출하여 5개의 영상을 이진 분류체계로 조합한 후 임상의 시기적 변화 양상을 관찰한 결과, 강원대 연습림의 조림 기록 및 현황도와 상당히 일치함을 확인하였으며, Path 115, Row 34에 해당하는 강원도 일대로 연구지역을 확대하였다. 향후 Kompsat-2를 비롯한 고해상도 11월 영상이 지속적으로 촬영된다면, 이 연구에서 개발된 이진 분류체계 방법을 통하여 산림변화의 모니터링을 보다 용이하고 효율적으로 할 수 있을 것으로 기대된다.

• 한국균학회지
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• 제46권3호
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• pp.205-211
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• 2018

Fungal endophytes are defined as fungi inhabiting plant tissues, causing no apparent disease. As the agricultural and ecological importance of endophytes has increased, many studies have been performed on various aspects, ranging from basic identification, taxonomy, and evolution to secondary metabolites with potential for human use. In the Korean Peninsula, conifers are the most commonly found evergreen trees, with approximately 30 to 60 reported taxa. Studies on the effect of conifer-associated endophytes on the host plants are required for the preservation and conservation of coniferous forests which decline by climate change and deforestation. This review summarizes the diversity of endophytic fungi in coniferous trees of Korea and their relationship with host plants.

• 대한기계학회:학술대회논문집
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• 대한기계학회 2000년도 추계학술대회논문집B
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• pp.381-384
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• 2000

Relationships between biochemical phenomena and hemodynamics on human endothelial cells are very important to study the mechanism of atherosclerotic formation and development. The objective of this study is to investigate the flow phenomena around the endothelial cell model by the PIV experiment. The microscopic images of endothelial cells were acquired by a CCD camera to fabricate the shape of endothelial cell. The cell models were fabricated by using a photoforming process. Two consecutive particle images were captured by the CCD camera for the image processing. Conifer powder as the tracing particles was added to water to visualize the flow field. The cross-correlation method was applied fer the image processing of the flow visualization. Pressure and wall shear stress variations on the surfaces of the endothelial cells were calculated to investigate the effects of hemodynamic forces on the morphological changes.

• The Plant Pathology Journal
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• 제21권1호
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• pp.1-6
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• 2005

During last decade there has been noticeable progress in the research of the biology of sapstaining fungi that cause considerable economic losses to forest product industry. The researches generated broad ranges of knowledge on sapstaining fungi regarding their occurrence on conifer wood, taxonomy, nutrient physiology, pigmentation biochemistry and molecular biology, and biological control. Major problematic groups in the sapstain production are Ophiostoma, Ceratocystis, and Leptographium genera. With Ophiostoma as a model, it is found that the type of carbon source is important in the growth and pigment production of sapstaining fungi. The operation of dihydroxy naphthalene (DHN) melanin pathway for black to bluish pigment production has been confirmed in those cosmetic fungi both at biochemical and molecular levels. The development of albino technology using nutrition competition has been shown to be promising as an environmentally friendly biological control method for sapstain control.