• Title/Summary/Keyword: Confocal system

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Feasibility Study for the Development of a Device for Detecting Pathological Tissues (병리학적 조직 진단장치 개발에 대한 타당성 분석 연구)

  • Ko, Chea-Ok;Park, Min-Young;Pack, Jeong-Ki
    • Proceedings of the Korea Electromagnetic Engineering Society Conference
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    • 2005.11a
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    • pp.421-424
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    • 2005
  • X-ray is currently most effective method in detecting small malignant breast tumors but has the several problems due to suppressing breast, ionizing radiation and not detecting small cancer. In this paper, a new method is proposed by using dielectric characteristics of pathological tissues and time delay of backscattered response. We have developed a detection algorithm and verified it by numerical simulation and measurement for a prototype system. For a prototype system, we have fabricated experimental model(artificial breast with a cancer) and UWB(ultra-wideband) antenna. The results of the measurement simulation show an excellent detection capability of a cancer tissue. It is found that a good UWB antenna is a key element of such detection system. Further study is ongoing to develop a commercial system.

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Standoff Raman Spectroscopic Detection of Explosive Molecules

  • Chung, Jin Hyuk;Cho, Soo Gyeong
    • Bulletin of the Korean Chemical Society
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    • v.34 no.6
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    • pp.1668-1672
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    • 2013
  • We developed a standoff Raman detection system for explosive molecules (EMs). Our system was composed of reflective telescope with 310 mm diameter lens, 532 nm pulse laser, and Intensified Charge-Coupled Device (ICCD) camera. In order to remove huge background noise coming from ambient light, laser pulses with nanosecond time width were fired to target sample and ICCD was gated to open only during the time when the scattered Raman signal from the sample arrived at ICCD camera. We performed standoff experiments with military EMs by putting the detector at 10, 20 and 30 m away from the source. The standoff results were compared with the confocal Raman results. Based on our standoff experiments, we were able to observe the peaks in the range of 1200 and $1600cm^{-1}$, where vibrational modes of nitro groups were appeared. The wave numbers and shapes of these peaks may serve as good references in detecting and identifying various EMs.

Development and validation of strip bending tester for measuring mechanical properties of freestanding thin films (자유지지 박막의 기계적 물성 측정을 위한 띠굽힘시험기의 개발 및 검증)

  • Park, Jung-Min;Kim, Jae-Hyun;Lee, Hak-Joo
    • Proceedings of the KSME Conference
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    • 2008.11a
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    • pp.49-55
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    • 2008
  • Strip bending test has been frequently utilized to measure the mechanical properties of freestanding thin films in substitute for the micro-tensile test. However, in spite of its simplicity and reliability, strip bending test has a few problems, for example, the measurement of strain and the calculation of stress at zero strain. In this study, these problems are precisely reviewed and proved. Upon this review, strip bending tester has been developed, which uses the confocal laser displacement meter to measure the deformed configuration of the specimen and the possibility and limitation of this testing system is carefully investigated including the estimation of uncertainty of the measurement of strain. Finally, to prevent errors and to improve the accuracy of this testing system, the shape of the specimen has been carefully studied and is proposed.

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INTERFACIAL MORPHOLOGY BETWEEN DENTIN AND ADHESIVES (상아질과 접착제 간의 계면양상)

  • Kang, Ji-Young;Hur, Bock
    • Restorative Dentistry and Endodontics
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    • v.20 no.1
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    • pp.183-192
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    • 1995
  • The purpose of this study was to evaluate the effect of smear layer management on the interfacial morphology between dentin bonding system and dentin. Clearfil New Bond, Scotchbond Multipurpose, Prisma Universal Bond 3 and X-R Bond were used on the cervical dentinal surfaces of bovine incisor teeth. All of the dentin bonding systems were labeled with fluorescene in primer and rhodamine B in adhesive. Specimens of 2~3mm thichness were prepared by longitudinal and labiolingual sectioning. The interface between dentin bonding system and dentin was observed by flouresence imaging with a confocal laser scanning microscope. Following results were obtained. 1. In the specimen of Clearfil New Bond, dentinal tubules were widened by destruction of peritubular dentin in the course of treatment with phosphoric acid of high concentration. 2. Hybrid layer was observed in the specimen of Scotchbond Multipurpose and X-R Bond. 3. In the specimen of Prisma Universal Bond 3, the penetraton of adhesive was not observed clearly.

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THERMALLY INDUCED PHASE SEPARATION IN TERNARY POLYMER SOLUTION

  • Jung, Bum-Suk;Kang, Yong-Soo;Jones, Richard-A.L.
    • Proceedings of the Membrane Society of Korea Conference
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    • 1999.07a
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    • pp.79-82
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    • 1999
  • Using Small Angle Light Scattering (SALS), the effect of quench depth on the kinetics of phase separation for ternary solution blends was investigated. The system was composed of two polymers (polystyrene and polybutadiene) and a solvent (toluene). The analyses of the early stage of phase separation were based of the Cahn-Hilliard theory [1,5]. Apparent diffusion coefficients and the fastest mode of fluctuations were evaluated, when quench depth of the system were varied near the critical composition of polymer. In the late stage of phase separation, the domain growth showed a power law with the 1/3 exponent, i.e. $q_m(t)~t^{-1/3}$. For comparison between real images and scattering profiles with time, the image of phase domains with time were obtained by using Laser Confocal Scanning Microscopy (LSCM).

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Optical Design and Tolerance Analysis for UVO-Multiband Polarizing Imager System

  • Han, Jimin;Chang, Seunghyuk;Park, Woojin;Lee, Sunwoo;Ahn, Hojae;Kim, Geon Hee;Lee, Dae-Hee;Pak, Soojong
    • The Bulletin of The Korean Astronomical Society
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    • v.45 no.1
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    • pp.68.2-68.2
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    • 2020
  • UVO-Multiband Polarizing Imager System (UVOMPIS) is an ultraviolet to visible light multi-wavelength polarization/imaging system for Compact Advanced Satellite. We developed Linear Astigmatism Free-Three Mirror System (LAF-TMS) D200F2 as an optical system of UVOMPIS which has an entrance pupil diameter of 200 mm, a focal ratio of 2, a field of view of 2° × 4°. LAF-TMS is a confocal off-axis reflecting telescope system that removes linear astigmatism, and its all mirrors (M1, M2, M3) are optimized with the freeform surface to reduce high-order aberrations. Through the sensitivity analysis and Monte-Carlo simulation as the tolerance analysis, we can confirm the feasibility of the system, relatively sensitive parameters (tilt, decenter, despace, surface RMS error), and considerations for optomechanical design. From the sensitivity analysis, we can discover the relatively sensitive optical alignment parameters to a single perturbation. Further more, in the monte-carlo simulation, we investigate the minimum tolerance budget satisfying the required optical performance and whether the tolerance range is satisfied within manufacturing error.

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Micro-imaging techniques for evaluation of plastic microfluidic chip

  • Kim, Jung-Kyung;Hyunwoo Bang;Lee, Yongku;Chanil Chung;Yoo, Jung-Yul;Yang, Sang-Sik;Kim, Jin-Seung;Park, Sekwang;Chang, Jun-Keun
    • JSTS:Journal of Semiconductor Technology and Science
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    • v.1 no.4
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    • pp.239-247
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    • 2001
  • The Fluorescence-Activated Cell Sorter (FACS) is a well-established instrument used for identifying, enumerating, classifying and sorting cells by their physical and optical characteristics. For a miniaturized FACS device, a disposable plastic microchip has been developed which has a hydrodynamic focusing chamber using soft lithography. As the characteristics of the spatially confined sample stream have an effect on sample throughput, detection efficiency, and the accuracy of cell sorting, systematic fluid dynamic studies are required. Flow visualization is conducted with a laser scanning confocal microscopy (LSCM), and three-dimensional flow structure of the focused sample stream is reconstructed from 2D slices acquired at $1\mutextrm{m}$ intervals in depth. It was observed that the flow structure in the focusing chamber is skewed by unsymmetrical velocity profile arising from trapezoidal cross section of the microchannel. For a quantitative analysis of a microscopic flow structure, Confocal Micro-PIV system has been developed to evaluate the accelerated flow field in the focusing chamber. This study proposes a method which defines the depth of the measurement volume using a detection pinhole. The trajectories of red blood cells (RBCs) and their interactions with surrounding flow field in the squeezed sample stream are evaluated to find optimal shape of the focusing chamber and fluid manipulation scheme for stable cell transporting, efficient detection, and sorting

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Improved dentin disinfection by combining different-geometry rotary nickel-titanium files in preparing root canals

  • Bedier, Marwa M.;Hashem, Ahmed Abdel Rahman;Hassan, Yosra M.
    • Restorative Dentistry and Endodontics
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    • v.43 no.4
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    • pp.46.1-46.10
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    • 2018
  • Objectives: This study was to evaluate the antibacterial effect of different instrumentation and irrigation techniques using confocal laser scanning microscopy (CLSM) after root canal inoculation with Enterococcus faecalis (E. faecalis). Materials and Methods: Mesiobuccal and mesiolingual canals of extracted mandibular molars were apically enlarged up to a size 25 hand K-file, then autoclaved and inoculated with E. faecalis. The samples were randomly divided into 4 main groups according to the system of instrumentation and irrigation: an XP-endo Shaper (XPS) combined with conventional irrigation (XPS/C) or an XP-endo Finisher (XPF) (XPS/XPF), and iRaCe combined with conventional irrigation (iRaCe/C) or combined with an XPF (iRaCe/XPF). A middle-third sample was taken from each group, and then the bacterial reduction was evaluated using CLSM at a depth of $50{\mu}m$ inside the dentinal tubules. The ratio of red fluorescence (dead cells) to green-and-red fluorescence (live and dead cells) represented the percentage of bacterial reduction. The data were then statistically analyzed using the Kruskal-Wallis test for comparisons across the groups and the Dunn test was used for pairwise comparisons. Results: The instrumentation and irrigation techniques had a significant effect on bacterial reduction (p < 0.05). The iRaCe/XPF group showed the strongest effect, followed by the XPS/XPF and XPS/C group, while the iRaCe/C group had the weakest effect. Conclusions: Combining iRaCe with XPF improved its bacterial reduction effect, while combining XPS with XPF did not yield a significant improvement in its ability to reduce bacteria at a depth of $50{\mu}m$ in the dentinal tubules.

Analysis of Rock Surface Roughness and Chemical Species Generation by Freeze-Thaw Experiments (동결융해 실험을 통한 암석 표면 거칠기 및 화학종 생성에 관한 분석)

  • Choi, Junghae
    • Korean Journal of Mineralogy and Petrology
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    • v.35 no.3
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    • pp.299-311
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    • 2022
  • Rocks exposed to the surface are subject to long-term weathering, and such effects weaken their engineering stability. Especially as weathering progresses, the surface of rocks will be changed by weathering, and such surface changes will affect the engineering safety of the rock mass. In addition, the chemical species produced in the weathered rock have a direct effect on the surrounding environment or on the structure. In areas where rocks are exposed, such as mining areas, chemical species produced by weathering can have a serious impact on the surrounding natural environment. In this study, accelerated weathering experiments using freeze/thaw system were conducted on rocks that had already been weathered and fresh rocks, and surface changes of each rock were observed with confocal laser scanning microscope (CLSM), and chemical species were analyzed using IC/ICP-MS. As the weathering progressed, the surface roughness decreased, and the amount of chemical species produced increased. The results of this study can be used as basic data for evaluating engineering/environmental safety in areas where rocks are exposed.

Bonding effects of cleaning protocols and time-point of acid etching on dentin impregnated with endodontic sealer

  • Tatiane Miranda Manzoli;Joissi Ferrari Zaniboni;Joao Felipe Besegato;Flavia Angelica Guiotti;Andrea Abi Rached Dantas;Milton Carlos Kuga
    • Restorative Dentistry and Endodontics
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    • v.47 no.2
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    • pp.21.1-21.11
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    • 2022
  • Objectives: This study aimed to investigate the bonding effects of cleaning protocols on dentin impregnated with endodontic sealer residues using ethanol (E) or xylol (X). The effects of dentin acid etching immediately (I) or 7 days (P) after cleaning were also evaluated. For bonding to dentin, universal adhesive (Scotchbond Universal; 3M ESPE) was used. The persistence of sealer residues, hybrid layer formation and microshear bond strength were the performed analysis. Materials and Methods: One hundred and twenty bovine dentin specimens were allocated into 4 groups (n = 10): G1 (E+I); G2 (X+I); G3 (E+P); and G4 (X+P). The persistence of sealer residues was evaluated by SEM. Confocal laser scanning microscopy images were taken to measure the formed hybrid layer using the Image J program. For microshear bond strength, 4 resin composite cylinders were placed over the dentin after the cleaning protocols. ANOVA followed by Tukey test and Kruskal-Wallis followed by Dunn test were used for parametric and non-parametric data, respectively (α = 5%). Results: G2 and G4 groups showed a lower persistence of residues (p < 0.05) and thicker hybrid layer than the other groups (p < 0.05). No bond strength differences among all groups were observed (p > 0.05). Conclusions: Dentin cleaning using xylol, regardless of the time-point of acid etching, provided lower persistence of residues over the surface and thicker hybrid layer. However, the bond strength of the universal adhesive system in etch-and-rinse strategy was not influenced by the cleaning protocols or time-point of acid etching.