• 제목/요약/키워드: Confocal laser microscope

검색결과 193건 처리시간 0.029초

공초점레이저주사현미경을 이용한 심미수복재와 상아질의 접착계면에 관한 연구 (A CONFOCAL LASER SCANNING MICROSCOPIC STUDY ON THE INTERFACE BETWEEN TOOTH COLORED RESTORATIVE MATERIALS AND DENTIN)

  • 박병철;조영곤;문주훈
    • Restorative Dentistry and Endodontics
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    • 제25권3호
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    • pp.313-320
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    • 2000
  • The purpose of this study was to evaluate on the interfacial morphology between dentin and restorative materials. In this in vitro study, the cavity wall restorated with 3 different kinds of tooth colored restorative materials [resin-modified Glass Ionomer cement (Fuji II LC), composite resin (Z-100), compomer (Dyract)]. The thirty extracted human molar teeth without caries and/or restorations are used. The experimental teeth were randomly divided into three groups of ten teeth each. In each group, Wedge shaped cavities (width: 3mm, length: 2mm, depth: 1.5mm) were prepared at the cementoenamel junction on buccal and lingual surfaces. The adhesive of composite resin were mixed with rhodamine B. Primer of composite resin, Prime & Bond 2.1 of Dyract and liquid of Fuji II LC were mixed with fluorescein. In group 1, the cavity wall was treatment with dentin conditioner, and then restorated with Fuji II LC. In group 2, the cavity wall was treatment with Prime & Bond 2.1 and then restorated with Dyract. In group 3, the cavity wall was etching with 10% maleic acid, applied with primer and bonding agent and then restorated with Z-100. The interface between dentin and restorative materials was observed by fluoresence imaging with a confocal laser scanning microscope. The results were as follows : 1. In Glass ionomer group, adaptation of resin modified Glass-ionomer restoration against cavity wall is tight, but the crack formed inside of restoration were observed. 2. In Dyract group, the penetration of resin tag is shorter and the width of hybrid layer is narrower than composite resin group. 3. In Z-100 group, primer penetrated deeply through dentinal tubule. Also bonding agent was penetrated along the primer, but the penetration length is shorter than primer part, and in 3-D image, the resin tag is conical shape and lateral branch is observed.

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ANALYSIS OF THE PERMEABILITY CHARACTERISTICS ALONG ROUGH-WALLED FRACTURES USING A HOMOGENIZATION METHOD

  • Chae, Byung-Gon;Choi, Jung-Hae;Ichikawa, Yasuaki;Seo, Yong-Seok
    • Nuclear Engineering and Technology
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    • 제44권1호
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    • pp.43-52
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    • 2012
  • To compute a permeability coefficient along a rough fracture that takes into account the fracture geometry, this study performed detailed measurements of fracture roughness using a confocal laser scanning microscope, a quantitative analysis of roughness using a spectral analysis, and a homogenization analysis to calculate the permeability coefficient on the microand macro-scale. The homogenization analysis is a type of perturbation theory that characterizes the behavior of microscopically inhomogeneous material with a periodic boundary condition in the microstructure. Therefore, it is possible to analyze accurate permeability characteristics that are represented by the local effect of the facture geometry. The Cpermeability coefficients that are calculated using the homogenization analysis for each rough fracture model exhibit an irregular distribution and do not follow the relationship of the cubic law. This distribution suggests that the permeability characteristics strongly depend on the geometric conditions of the fractures, such as the roughness and the aperture variation. The homogenization analysis may allow us to produce more accurate results than are possible with the preexisting equations for calculating permeability.

A STUDY OF THE PRESSURE SOLUTION AND DEFORMATION OF QUARTZ CRYSTALS AT HIGH pH AND UNDER HIGH STRESS

  • Choi, Jung-Hae;Seo, Yong-Seok;Chae, Byung-Gon
    • Nuclear Engineering and Technology
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    • 제45권1호
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    • pp.53-60
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    • 2013
  • Bentonite is generally used as a buffer material in high-level radioactive waste disposal facilities and consists of 50% quartz by weight. Quartz strongly affects the behavior of bentonite over very long periods. For this reason, quartz dissolution experiment was performed under high-pressure and high-alkalinity conditions based on the conditions found in a high-level radioactive waste disposal facility located deep underground. In this study, two quartz dissolution experiments were conducted on 1) quartz beads under low-pressure and high-alkalinity conditions and 2) a single quartz crystal under high-pressure and high-alkalinity conditions. Following the experiments, a confocal laser scanning microscope (CLSM) was used to observe the surfaces of experimental samples. Numerical analyses using the finite element method (FEM) were also performed to quantify the deformation of contact area. Quartz dissolution was observed in both experiments. This deformation was due to a concentrated compressive stress field, as indicated by the quartz deformation of the contact area through the FEM analysis. According to the numerical results, a high compressive stress field acted upon the neighboring contact area, which showed a rapid dissolution rate compared to other areas of the sample.

원전 고온 1차수 환경에서 응력부식균열의 실시간 마이크로 스케일 관찰 방법 개발 (Development of Method for In-situ Micro-Scale Observation of Stress Corrosion Cracking in High-Temperature Primary Water Environment)

  • 신정호;이종연;김성우
    • Corrosion Science and Technology
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    • 제22권4호
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    • pp.265-272
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    • 2023
  • The aim of this study was to develop a new in-situ observation method and instrument in micro-scale to investigate the mechanism of stress corrosion cracking (SCC) initiation of Ni-base alloys in a high temperature water environment of pressurized water reactors (PWRs). A laser confocal microscope (LCM), an autoclave with diamond window view port, and a slow strain-rate tester with primary water circulation loop system were components of the instrument. Diamond window, one of the core components of the instrument, was selected based on its optical, chemical, and mechanical properties. LCM was used to observe the specimen in micro-scale, considering the experimental condition of a high-temperature primary water environment. Using in-situ method and instrument, it is possible to observe oxidation and deformation of specimen surface in micro-scale through the diamond window in a high-temperature primary water in real-time. The in-situ method and instrument developed in this work can be utilized to investigate effects of various factors on SCC initiation in a high-temperature water environment.

Application of Confocal Laser Scanning Microscopy and Fiber Distribution Index to Study Kenaf Handsheet Properties

  • Pang, Myong-Hyeok;Park, Jong-Moon;Cho, Nam-Seok
    • 펄프종이기술
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    • 제31권3호
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    • pp.54-59
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    • 1999
  • This study was to quantify fiber distributions in thickness direction of kenaf handsheets as a fiber distribution index (FDI) and to analyze the relationship between FDI and the handsheet properties. The images of fiber distribution in z-direction were obtained by Confocal Laser Scanning Microscope (CLSM) and analyzed by image analysis technique. The proposed FDI had a good correlation with high R2 vlaues with various properties of paper, such as apparent density, scattering coefficient , burst index, tear index, tensile index, and folding endurance. The proposed FDI was shown as a good index to quantify paper properties.

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Reflection-type Optical Waveguide Index Profiling Technique

  • Youk YoungChun;Kim Dug Young
    • Journal of the Optical Society of Korea
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    • 제9권2호
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    • pp.49-53
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    • 2005
  • We report a new configuration of a reflection-type confocal scanning optical microscope system for measuring the refractive index profile of an optical waveguide. Several improvements on the earlier design are proposed; a light emitting diode (LED) at 650 nm wavelength instead of a laser diode (LD) or He-Ne laser is used as a light source for better index precision, and a simple longitudinal linear scanning and curve fitting techniques are adapted instead of a servo control for maintaining an optical confocal arrangement. We have obtained spatial resolution of 700 nm and an index precision of $2\times10^{-4}$. To verify the system's capability, the refractive index profiles of a conventional multimode fiber and a home-made four-mode fiber were examined with our proposed measurement method.