• Title/Summary/Keyword: Confluent education

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Effects of Transforming Growth Factor Beta on Cytoskeleton Structure and Extracellular Matrix in Mv1Lu Mink Epithelial Cells

  • Choi, Eui-Yul;Lee, Kyung-Mee;Chung, So-Young;Nham, Sang-Uk;Yie, Se-Won;Chun, Gie-Taek;Kim, Pyeung-Hyun
    • BMB Reports
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    • v.29 no.5
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    • pp.405-410
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    • 1996
  • Previous studies have shown that transforming growth factor beta ($TGF-{\beta}$) is a potent regulator of cell growth and differentiation. To study the effects of $TGF-{\beta}$ on cell morphology and cytoskeleton reorganization, we conducted a survey using Mv1Lu mink lung epithelial cells with antibodies to cytoskeletal proteins and an extracellular matrix protein. While the untreated cells showed a cuboidal shape of typical epithelia, the Mv1Lu cells displayed a drastic shape change in the presence of $TGF-{\beta}$. This alteration was most prominent when near-confluent cells were treated with $TGF-{\beta}$. Since the morphology alteration is known to be accompanied by the reorganization of cytoskeletal proteins in other cell types, we investigated the intracellular distribution of the three major cytoskeletal structures: microfilaments, microtubules, and intermediate filaments. In the microfilament system, $TGF-{\beta}$ induced new stress fiber formation, which was caused primarily by the polymerization of cytoplasmic G-actin. However, $TGF-{\beta}$ appeared not to induce any significant changes in microtubular structures and vimentin filaments as determined by indirect fluorescence microscopy. Finally we confirmed the rapid accumulation of fibronectin by immunoblot analysis and chased the protein locations by immunofluorescence microscopy.

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Flume Experiments on Channel Morphology at a Tributary Junction (하천 합류점의 하도형상에 관한 수로실험)

  • Taeho Kim
    • Journal of the Korean Geographical Society
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    • v.33 no.3
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    • pp.355-364
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    • 1998
  • Flume experiments are conducted to describe the channel morphology at a tributary junction and to examine the influence of channel arrangements and hydrologic conditions on the channel morphology. When flow momenta of two tributaries are equal, a receiving stream tends to align with an axis bisecting junction angle. It causes lateral migration of a receiving stream according to an initial channel arrangement. As a result, the post-fonfluent channel morphology varies with plan geometry of a confluence such as symmetry, transition and asymmetry. Bed scour is the most notable morphology within a junction site. Its shape is characterized by steep walls which are primarily influenced by junction angle. Key control of scour dimension is also junction angle. Although the principle of accordant junction has been undoubtedly accepted, discordance is commonly developed at model and natural stream confluences. Unit discharge ratio of confluent streams is the most crucial factor because both discharge and sediment concentration ratios have an effect on discordance at a junction.

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Determining the Locations of Washland Candidates in the Four Major River Basins Using Spatial Analysis and Site Evaluation (공간분석 및 현장조사 평가 기법을 활용한 4대강 강변저류지 조성 후보지 선정)

  • Jeong, Kwang-Seuk;Shin, Hae-Su;Jung, Ju-Chul;Kim, Ik-Jae;Choi, Jong-Yun;Jung, In-Chul;Joo, Gea-Jae
    • Korean Journal of Ecology and Environment
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    • v.43 no.1
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    • pp.44-54
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    • 2010
  • In this study, a comprehensive exploration and evaluation of washland candidate locations by means of field monitoring as well as spatial analysis in six major river system (Han, Nakdong, Nam, Geum, Youngsan, and Seomjin Rivers). Washland(in other words, river detention basin) is an artificial wetland system which is connected to streams or rivers likely to riverine wetlands. Major purpose of washland creation is to control floodings, water supply and purification, providence of eco-cultural space to human and natural populations. Characteristics and functions of riverine wetlands can be expected as well, thus it is believed to be an efficient multi-purpose water body that is artificially created, in terms of hydrology and ecology. Geographical information and field monitoring results for the washland candidate locations were evaluated in 2009, with respect to optimal location exploration, ecosystem connectivity and educational-cultural circumstances. A total of $269\;km^2$ washland candidate locations were found from spatial analysis (main channel of Rivers South Han, 71.5; Nakdong 54.1; Nam, 2.3; Geum, 79.0; Youngsan 46.4; Seomjin 15.7), and they tended to be distributed in mid- to lower part of the rivers to which tributaries are confluent. Field monitoring at 106 sites revealed that some sites located in the Rivers Nam and Geum is appropriate for restoration or artificial creation as riverine wetlands. Several sites in the Nakdong and Seomjin Rivers were close to riverine wetlands (e.g., Upo), habitats of endangered species (e.g., otters), or adjacent to educational facility (e.g., museums) or cultural heritages (e.g., temples). Those sites can be utilized in hydrological, ecological, educational, and cultural ways when evidence of detailed hydrological evaluation is provided. In conclusion, determination of washland locations in the major river basins has to consider habitat expansion as well as hydrological function (i.e. flood control) basically, and further utility (e.g. educational function) will increase the values of washland establishment.