• Journal of Wetlands Research
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• v.4 no.1
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• pp.63-85
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• 2002

This study was carried out to provide basic data for water environmental control and conservation, and to elucidate monthly water environmental factor, phytoplankton flora and dominant species that each stations was maked a selection of a sites by represent 3 branch-river in the Taejon area from Nov. 1997 to Dec. 1998. Range of water temperature was $2.4^{\circ}C{\sim}30.9^{\circ}C$(means of $16.02^{\circ}C{\sim}18.92^{\circ}C$), pH was 7.1~10.4(means of 7.40~8.68). BOD was generally under $5mg/{\ell}$ except Gap-chon kyo in Nov. and COD was measured highly by interfer of men at the each sites. Especially, occasion of Gap-chon kyo, data was estimated about 3~13 times higher than another sites. DO was showed 1.03~10.43ppm, SS was showed $1.0{\sim}120.0mg/{\ell}$ TN was that, upper stream was 0.63ppm, middle was 0.35ppm and then lower was 0.44ppm, upper stream was higher than lower. TP was at upper stream 0.03ppm, middle 0.05ppm, lower 0.06ppm, lower stream was higher than another. The phytoplankton flora was identified as total 230 taxa composed of 7 classes, 18 oders 35 families, 75 genara, 184 species, 42 virieties, 4 forms. There are composed of that, Chlorophyceae 94 taxa(40.9%), Bacillariophyceae 66 taxa(28.8%), Euglenophyceae 39 taxa(17.0%), Cyanophyceae 24 taxa(10.4%), Chrysophyceae 4 taxa(1.7%), Rhodophyceae 2 taxa(0.8%) Xanthophyceae 1 taxa(0.4%), composition of Spesies of each sites was Gapchon-kyo 119, Wonchon-kyo 94, Gasuwon-kyo and Mannyon-kyo 87, Ochung-kyo 78, Hyunam-kyo and Suchim-kyo 77, Moonchang-kyo 70 taxa and Sansung-kyo 58 taxa. Number of species was showed tendency of increase lower stream. Taejon-chon and youdung-chon and at the sites of confluence were presented Synedra ulna and Diatom vugare like bacillariophyceae, Gap-chon was dominated variously Oscillatoria princeps and chlorophyceae, confluence of 3 branch-river was presented Euglenophyceae.

• Journal of Embryo Transfer
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• v.18 no.3
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• pp.171-178
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• 2003

This study was conducted to investigate the effect of electric fusion methods on cell fusion rate and embryo development by somatic cell nuclear transfer in Korean Native Cattle. The KNC ear cell was cultured in vitro for confluence in serum starvation condition(DMEM＋0.05％ FBS) for cell confluence. The zona pellucida of IVM oocytes were partially dissection using micro pipette. Ear cells were transferred into an enucleated oocyte. The reconstructed embryos were electrically fused with Zimmermann Cell Fusion Medium(ZCFM). Nuclear transfer embryos were activated with a combination of 10${\mu}{\textrm}{m}$ calcium ionophore(5 min) and 2.0mM 6-DMAP(3 hr). The activated embryos were cultured in CR1 -aa medium contains 0.3％ BSA or 10％ FBS at 37$^{\circ}C$, 90％ $N_2$, and 5％ $CO_2$in incubator for 6 days. The fusion rates were 51.6％(chamber) and 68.9％(needle), respectively and there were significantly difference between the fusion method(P<0.05). But, lysis rates were not significantly different(10.7％, 11.5％), respectively. The cleavage rates were significantly different between the chamber method(73.2％) and needle method(80.3％), respectively(P<0.05). The rates of early embryos(2∼4cells) and blastocysts of chamber and needle methods were 54.1％, 61.1％ and 18.4％, 26.3％ respectively, and needle method was significantly higher than chamber method(P<0.05). But, morulae formation rate were not significantly differences between the chamber(6.7％) and needle(6.2) method(P <0.05). These result suggest that electric fusion of needle method was to be profitable for nuclear transfer embryo fusion rate, blastocyst formation rate and reduce of oocyte lysis.

• Journal of Korea Water Resources Association
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• v.56 no.3
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• pp.195-210
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• 2023

Additional deposition and erosion in large rivers in South Korea have continued to occur toward morphological stabilization after massive dredging through the four major river restoration project, subsequently requiring precise bathymetry monitoring. Hyperspectral bathymetry method has increasingly been highlighted as an alternative way to estimate bathymetry with high spatial resolution in shallow depth for replacing classical intrusive direct measurement techniques. This study introduced the conventional Optimal Band Ratio Analysis (OBRA) of hyperspectral bathymetry method, and evaluated the performance in a domestic large river in normal turbid and flow condition. Maximum measurable depth was estimated by applying correlation coefficient and root mean square error (RMSE) produced during OBRA with cascadedly applying cut-off depth, where the consequent hyperspectral bathymetry map excluded the region over the derived maximum measurable depth. Also non-linearity was considered in building relation between optimal band and depth. We applied the method to the Nakdong and Hwang River confluence as a large river case and obtained the following features. First, the hyperspectal method showed acceptable performance in morphological mapping for shallow regions, where the maximum measurable depth was 2.5 m and 1.25 m in the Nakdong and Hwang river, respectively. Second, RMSE was more feasible to derive the maximum measurable depth rather than the conventional correlation coefficient whereby considering various scenario of excluding range of in situ depths for OBRA. Third, highly turbid region in Hwang River did not allow hyperspectral bathymetry mapping compared with the case of adjacent Nakdong River, where maximum measurable depth was down to half in Hwang River.

• Journal of The Korean Dental Society of Anesthesiology
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• v.14 no.1
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• pp.41-47
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• 2014

Background: Autophagy is a self-eating process that is important for balancing sources of energy at critical times in development and in response stress. Autophagy also plays a protective role in removing clearing damaged intracellular organelles and aggregated proteins as well as eliminating intracellular pathogens. The purpose of the present study was to examine the protective effect of propofol against hypoxic damage using keratinocytes. Methods: Human keratinocytes (HaCaT cells) were obtained from the American Type Culture Collection. Propofol which were made by dissolving them in DMSO were kept frozen at $-4^{\circ}C$ until use. The stock was diluted to their concentration with DMEM when needed. Prior to propofol treatment cells were grown to about 80% confluence and then exposed to propofol at different concentrations (0, 25, 50, 75, $100{\mu}M$) for 2 h pretreatment. Cell viability was measured using a quantitative colorimetric assay with thiazolyl blue tetrazolium bromide (MTT assay), and fluorescence microscopy and western blot analysis were used for evaluation of autophagy processes. Results: The viability of propofol-treated HaCaT cells was increased in a dose-dependent manner. Propofol did not show any significant toxic effect on the HaCaT cells. The autophagy inhibitor, 3-methyladenine, reduced cell viability of hypoxia-injured HaCat cells. Fluorescence microscopy and western blot analysis showed propofol induce autophagy pathway signals. Conclusions: Propofol enhanced viability of hypoxia-injured HaCaT cells and we suggest propofol has cellular protective effects by autophagy signal pathway activation.

• Journal of the Korean Society for Railway
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• v.15 no.6
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• pp.631-637
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• 2012

New Opening of Suseo-Pyeongteak High Speed Railway (HSR) will be a new leap in the Korean railway history. However if this section of HSR line around Seoul Metropolitan Area opens, the confluence of new HSR and existing HSR line in Pyeongteak-Osong section will cause a bottle neck problem. In other words, the opening of Suseo-Pyeongteak HSR line will make the capacity of track reach the limit and the section of railroad between Pyeongteak and Osong will be saturated. This will also make such troubles as restricting the number of train which stops at Cheonan-Asan station. In this study, based on the train assignment theory of TVM430 signal system, the methods of calculating headway and number of train are reviewed and the plan for application of optimal operation pattern during peak hour between Pyeongteak-Osong section is also suggested. To remove the bottle neck problem in this HSR section, 3 alternatives are suggested and the expected effects and problems of each alternative are also analyzed. The results show that the troubles caused by excess of track capacity can be removed without any additional cost if the minimum headway in operating system for HSR is adopted in this section. In the future, if these alternatives are considered to the long-term plan for operating train and signal systems, this will improve the efficiency of train operation, which can remove the bottle neck in the HSR line.

• Journal of Wetlands Research
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• v.18 no.4
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• pp.363-377
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• 2016

This study was investigated the pollutant load, contamination properties, pollution condition of the fine parts of tributary, the influence of Nakdong river watershed and etc. in the tributaries. The contaminated tributaries were that among the Kumho and Nam river or were too far from site of water quality monitoring stations, regularly. As a result, the water quality level was almost similar between Nam and Kumho River, except for certain parameter including TN(Total Nitrogen), Chl-a(Chlorophyll-a) and SS(Suspended Solid) in which Kumho river were 20~120%. The point discharge load(kg/day) and load density ($kg/day/km^2$) of tributaries were different the pollution level according to the flow-rate ($m^3/sec$) and stream influence area($km^2$), and the difference of these was observed highly at Nam river. Specific contamination investigation of tributaries in Nam and Kumho river watershed was conducted from two to nine points of the fine parts of tributaries depending on the confluence sites and shapes. This result observed high at the Dalseocheon and Uriyeongcheon, respectively. Beside, the pollutant load contribution rate of Nakdong watershed was high about 10% at the Dalseocheon and Uiryeongcheon. This was due to the differences of the environments about the industrial complex, metropolis residence property, agricultural cultivation, livestock pen and the downstream of non-point source.

• Asian-Australasian Journal of Animal Sciences
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• v.29 no.3
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• pp.419-427
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• 2016

There is a high association of heat shock on the alteration of energy and lipid metabolism. The alterations associated with thermal stress are composed of gene expression changes and adaptation through biochemical responses. Previous study showed that Angelica gigas Nakai (AGN) root extract promoted adipogenic differentiation in murine 3T3-L1 preadipocytes under the normal temperature condition. However, its effect in heat shocked 3T3-L1 cells has not been established. In this study, we investigated the effect of AGN root hot water extract in the adipogenic differentiation of murine 3T3-L1 preadipocytes following heat shock and its possible mechanism of action. Thermal stress procedure was executed within the same stage of preadipocyte confluence (G0) through incubation at $42^{\circ}C$ for one hour and then allowed to recover at normal incubation temperature of $37^{\circ}C$ for another hour before AGN treatment for both cell viability assay and Oil Red O. Cell viability assay showed that AGN was able to dose dependently (0 to $400{\mu}g/mL$) increase cell proliferation under normal incubation temperature and also was able to prevent cytotoxicity due to heat shock accompanied by cell proliferation. Confluent preadipocytes were subjected into heat shock procedure, recovery and then AGN treatment prior to stimulation with the differentiation solution. Heat shocked preadipocytes exhibited reduced differentiation as supported by decreased amount of lipid accumulation in Oil Red O staining and triglyceride measurement. However, those heat shocked preadipocytes that then were given AGN extract showed a dose dependent increase in lipid accumulation as shown by both evaluation procedures. In line with these results, real-time polymerase chain reaction (RT-PCR) and Western blot analysis showed that AGN increased adipogenic differentiation by upregulating heat shock protection related genes and proteins together with the adipogenic markers. These findings imply the potential of AGN in heat shock amelioration among 3T3-L1 preadipocytes through heat shock factor and proteins augmentation and enhanced adipogenic marker expression.

• The Journal of Engineering Geology
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• v.13 no.1
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• pp.29-50
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• 2003

Physicochemical Properties of acid mine water of the Chonam-ri Creek and the Sagok-ri Creek in the Kwangyang Au-Ag mine area were determined using geochemical approaches. Metal contamination (Cd, Cu, Pb, Zn) is more serious in the Chonam-ri Creek than in the Sagok-ri Creek. However, the contents of Al and Fe is higher in the Sagok-ri Creek. Such differences between the two creeks probably reflect the abundance and composition of ore minerals. The attenuation processes for acid mine water in both creeks were investigated. In the Chonam-ri Creek, a small retention pond which contains limestone plays an important role in the removal of heavy metals by adsorption or coprecipitation due to increase of pH. The capacity of metal scavenging in this pond depends on the seasonal variation of inflow volume. Reddish yellow precipitates sampled in the Chonam-ri Creek were analyzed by XRD, SEM-EDS, EPMA, and chemical decomposition. The precipitates mainly consist of goethite and are also enriched in Al, Mn, Cu and Zn. This inditates that precipitation of goethite is important for scavenging those trace elements, possibly due to adsorption or coprecipitation. In the Sagok-ri Creek, on the other hand, hydrologic mixing of uncontaminated tributaries results in removal of heavy metals with iron hydroxides precipitation due to the pH increase. The mechanisms proposed for metal attenuation at the confluence between contaminated mine water and uncontaminated tributary water are also explained by the property-property plots.

• Imaging Science in Dentistry
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• v.35 no.3
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• pp.127-131
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• 2005

Purpose : We performed the present study to investigate whether osteotropic hormomes play roles on the nitric oxide (NO) production in culture of ROS 17/12.8 osteoblastic cells. Materials and Methods : The osteoblastic cell line ROS17/2.8 cells were cultured In F12 medium supplemented with $5\%$ fetal bovine serum (FBS) at $37^{\circ}C$ in a humidified atmosphere of $5\%\;CO_2$ in air. ROS17/2.8 cells were plated in 96-well plates at a density of $2-3\times10^3cells/well$ and grown to confluence. Then the cells were pretreated with osteotropic hormones (parathyroid hormone (PTH) 20-500 ng/mL, 1, 25-dihydroxycholecalciferol $(1,\;25[OH]_2D_3)$ 1-100 nM; prostaglandin $E_2 (PGE_2)$ 20-500 ng/mL in the medium supplemented with $0.4\%$ FBS for 72 hours and the cells were treated with cytokines $(TNF{\alpha}\;and\;IFN{\gamma})$ in phenol red-free F12 medium for an additional 48 hours. NO synthesis was assessed by measuring the nitrite anion concentration, the reaction product of NO, in the cell culture medium using Griess reagent. Results : PTH and $1,\;25[OH]_2D_3$ pretreatment induced a significant increase in NO production in the presence of $TNF{\alpha}\;and\;IFN{\gamma}.\;PGE_2$ slightly induced NO production compared to the control group. But, $PGE_2$ pretreatment did not affect in NO production in the presence of $TNF{\alpha}\;and\;IFN{\gamma}$. Conclusions : These results suggest that the actions of osteotropic hormones In bone metabolism may be partially mediated by NO in the presence of cytokines.

• Economic and Environmental Geology
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• v.27 no.1
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• pp.101-113
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• 1994

Geochemical investigations based on measurements of water parameters and sampling of stream sediments have been carried out, in the Okdongcheon stream and its tributaries in the Sangdong area of South Korea. There are two main problems occurring in the Okdongcheon stream: an acid mine drainage in the upper reaches and toxic trace metal contamination of the stream sediments mainly in the lower reaches. Acid mine water originating from coal mining was neutralized at the confluence of the Cheonpyongcheon stream whilst suspended solids due to flocculation of iron in water caused turbidity which was undesirable. Sediments in the Okdongcheon stream have been contaminated by mining activites. Iron was heavily concentrated in sediments in the upper Okdongcheon whilst toxic trace metals including Pb, Cu, Zn, Co, Cd, As and Bi were accumulated in sediments at stations draining metallic mining areas and near the tailings dam. There is now a requrement to neutralise the acid mine drainage and to use site-specific analysis of biological communities to ensure the conservation and preservation of aquatic organisms.