• Preventive Nutrition and Food Science
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• v.21 no.3
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• pp.236-244
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• 2016

In this study, we compared the anti-inflammatory activity of Pinus koraiensis cone bark extracts prepared by conventional extraction and microwave-assisted extraction (MAE). Water extracts and 50% ethanol extracts prepared using MAE were applied to RAW 264.7 cell at 5, 10, 25, and $50{\mu}g/mL$ of concentrations, and tested for cytoxicity. The group treated with $50{\mu}g/mL$ of 50% ethanol extracts showed toxicity. In order to investigate the inhibition of nitric oxide (NO) production in RAW 264.7 cells, extracts of water and ethanol were treated with 5, 10, and $25{\mu}g/mL$ concentrations. The inhibitory activity of water and 50% ethanol extracts groups were determined as 40% and 60% at $25{\mu}g/mL$ concentration, respectively. We found concentration dependent decreases on inducible NO synthase. The inhibitory effect against forming inflammatory cytokines, prostaglandin $E_2$, tumor necrosis factor-${\alpha}$, interleukin (IL)-6, and IL-$1{\beta}$, was also superior in the $25{\mu}g/mL$ treated group than the control group. According to these results, the water extracts and 50% ethanol extracts both inhibited inflammatory mediators by reducing the inflammatory response. Therefore, The MAE extracts of P. koraiensis cone bark can be developed as a functional ingredient with anti-inflammatory activity.

• Applied Microscopy
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• v.26 no.4
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• pp.421-430
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• 1996

The endocrine cells in the midgut epithelium of the Japanese cockroach, Periplaneta japonica were observed by the light and electron microscopy. The midgut epithelium of the last instar larva and adult cockroach consisted of principal columnar cells, regenerative cells, and secretory granular cells. Midgut endocrine cells were positioned basally as a cone-shaped single cell in the epithelium or underneath the regenerative crypt cells. When midgut epithelium grows and the cell composing it transforms, between the endocrine cells and regenerative cells were made desmosome type junction and large vesicular shaped stretches of loose contact. The endocrine cells were characterized by a clear cytoplasm with abundant Golgi complex and numerous secretory granules. The secretory granules in the cell were spherical and electron dense with their diameter of $200{\sim}400nm$. The secretory granules have been observed as discharged by exocytosis on the basal and lateral side of the cell.

• Imaging Science in Dentistry
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• v.47 no.3
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• pp.209-213
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• 2017

Central giant cell lesions are rare, benign, osteolytic, pseudocystic, solitary, localized lesions that are common in the skeletal structure, but less so in the maxillofacial region. Furthermore, to perform panoramic radiography and cone-beam computed tomography, it is necessary to prepare patients properly and to position their heads carefully. However, this can be difficult in pediatric patients, who may be anxious. In this report, we describe the case of a central giant cell lesion of the mandible in a 2-year-old girl that was evaluated with multidetector computed tomography.

• Korea-Australia Rheology Journal
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• v.19 no.4
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• pp.227-232
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• 2007

The use of various shearing apparatuses to study the phase behavior of poly(styrene-b-isoprene) diblock copolymer micelles is described. A DMTA rheometer was modified so that one can apply oscillatory shear and obtain the scattering pattern along the shear gradient direction. A cone and plate shear cell was designed to access scattering along the shear vorticity direction, and both oscillatory and steady shear can be applied. The most popular way to employ steady shear on relatively low viscosity fluids is to use a Couette cell, because a high shear rate can be readily achieved without disturbing the sample by overflow. In this work, oscillatory shear was used to obtain a single crystal-like scattering pattern, and thereby to examine the mechanism of the thermotropic transition between face-centered cubic (fcc) and body-centered cubic (bcc) lattices. By applying the steady shear, the response of the fcc lattices to various shear rates is discussed.

• The Journal of Korean Physical Therapy
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• v.12 no.3
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• pp.415-432
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• 2000

In mammals. axotomy of peripheral nerve leads to a complex. These events include swelling of cell body, disappearance of Nissl substance. Proximal and distal axon undergoes a variable deriable degree of traumatic degeneration and wallerian degeneration, respectively. Nerve injury may result in cell death or regeneration. Molecular changes include proliferation of Schwann cells, upregulation of neurotropism, neural cell adhesion molecules and cytokine. Also growth cone plays an essential role in axon guidance through interaction of cytoskeleton. We review cellular and molecular events after nerve injury and describe nerve regeneration and associated proteins.

• Korean Journal of Ichthyology
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• v.29 no.3
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• pp.218-223
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• 2017

Using both light and scanning electron microscopies, it was investigated on the visual cells as well as the eyes of Macropodus ocellatus (Pisces, Osphronemidae). This species had a circular lens and yellowish cornea. The eyes had $3.5{\pm}0.2mm$ which is $31.1{\pm}3.0%$ in a percentage of eye diameter relative to head length. The retina ($158.2{\pm}10.6{\mu}m$) was built of several layers, including the visual cell layer which consists of three types of cells: single cons ($27.8{\pm}1.6{\mu}m$) and equal double cone ($33.9{\pm}3.7{\mu}m$), and large rods ($57.3{\pm}1.3{\mu}m$). The visual cell layer then was classified into the correct pattern. All visual cells were clearly distinguished from two parts (inner and outer segments). The elongated rod cells were extend to the bottom of the retinal pigment epithelium. In scanning electron microscopy, the outer segment links to inner segment by so-called calyceal piles. The M. ocellatus single and double cones appearance form a flower-petal arrangement, which is a regular mosaic pattern that contains quadrilateral units by four double cones surrounding a single cone.

• Journal of Plant Biology
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• v.39 no.2
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• pp.137-144
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• 1996

Scanning electron microscope revealed several taxonomically useful characters in the perigynium and achene epidermis of sect. Siderosticatae (Carex siderosticta, C. ciliatomarginata and C. okamotoi). Mocroscopic characters such as perigynium shape, hair presence or absence, beak shape, nodule situation in perigynium, achene shape, cell wall and silica cone in achene were useful in Carex at the species levels. A key was presented on the basis of features obtained from the examinations.

• Korean Membrane Journal
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• v.5 no.1
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• pp.10-17
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• 2003

1,3,5-Sustitituted polyphenylene type dendrimers were synthesized. The dendrimer was heated in cone. H$_2$SO$_4$ at 120$^{\circ}C$. The reaction mixture was precipitated to ether after simple filtration to get para-sulfonated dendrimer as the chief product. To give mechanical properties to the sulfonated dendrimer, it was partially cross-linked with phenyl ether in the medium of P$_2$O$\sub$5/ and CH$_3$SO$_3$H.

• Anatomy and Cell Biology
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• v.56 no.2
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• pp.185-190
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• 2023

The root canal morphology undergoes aging-related changes, and relevant quantitative analyses have not yet been reported. We compared the cone beam computed tomography (CBCT) and micro-computed tomography (microCT) scans of extracted mandibular incisors to check the accuracy of morphological measurements. Thereafter, the root canal morphology and aging-related changes in the mandibular incisors of Japanese individuals were assessed using CBCT. Six extracted teeth were fixed in a phantom head and imaged using CBCT and micro-CT. The correlation between the findings of the two imaging modalities was examined. Further, CBCT reconstructed images of the mandibular incisors of 81 individuals were observed. Age-related changes of the root canals were compared between participants aged <30 years and those aged ≥30 years. The CBCT and micro-CT findings regarding the root canals of the extracted teeth coincided in 94.4% of the cases. Mandibular incisors exhibiting two root canals in either cross-section accounted for 9.9% of central incisors and 12.4% of lateral incisors. Mandibular central incisors with two root canals were observed in two (6.3%) individuals aged <30 years and six (12.2%) aged ≥30 years. Mandibular lateral incisors with two root canals were observed in one (3.1%) individual aged <30 years and nine (18.4%) aged ≥30 years. CBCT allows accurate evaluation of complex root canal morphologies and is useful for endodontic preoperative assessment. Mandibular incisors have more frequent occurrence of two root canals with aging.

• Animal Systematics, Evolution and Diversity
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• v.40 no.3
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• pp.211-220
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• 2024

During the surveys of ciliates from hypoxic habitats, three marine anaerobic species were found: Metopus spiculatus, M. vestitus, and Muranothrix felix. These species have not been previously recorded in South Korea and belong to the taxonomic classes Armophorea and Muranotrichea. The morphology of these species was examined by both microscopic observations of live specimens, and stained cells using protargol impregnations. Metopus spiculatus has the following characteristics: body size 80-110×25-35 ㎛ in vivo, beak-like structure at the end of preoral dome, ectosymbiotic bacteria covering cell surface, intracytoplasmic needle-shaped structures and the conspicuous tail end. Metopus vestitus has the following distinguishing characteristics: body size 95-130×25-45 ㎛ in vivo, a cone-shaped body, a covering of ectosymbiotic bacteria on its cell surface, intracytoplasmic needleshaped structures, somatic kineties arranged in 26-28 longitudinal rows, and a posterior part tapered into a tail. Muranothrix felix has the following characteristics: body size 100-130×20-30 ㎛ in vivo, elongated body with twisted neck region, bristle-like cilia protruding perpendicular to the cell margin, ectosymbiotic bacteria covering the cell surface, about 10 macronuclear nodules, and a long, stiffened caudal cilium.