• Korean Journal of Fisheries and Aquatic Sciences
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• v.54 no.1
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• pp.23-30
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• 2021

To examine the optimal temperature, light intensity, and shell-type for shell-living conchocelis production, we tested the shell infiltration of free-living conchocelis fragments under various environmental conditions. Under a combination of various temperatures (10, 15, 20, 25 and 30℃) and light intensities (1, 5, 10, 20, 40, and 80 μmol m-2 s-1), the optimal infiltration conditions of the evaluated three Pyropia species were 20-25℃ and 5-80 μmol m-2 s-1 for P. yezoensis, 20-30℃ and 20-80 μmol m-2 s-1 for P. seriata, and 20-25℃ and 20-80 μmol m-2 s-1 for P. dentata. The infiltration efficiency of free-living conchocelis for different shell types was greater in Korean and Chinese oyster Crassostrea gigas shells than that in scallop Argopecten irradians and clam Meretrix lusoria shells. These results suggest that oyster shells are suitable substrates for shell-living conchocelis production. In conclusion, the present results for optimal infiltration conditions for free-living conchocelis of the three examined Pyropia species will contribute significantly to the production of stable shell-living conchocelis.

• Proceedings of the Korean Society of Fisheries Technology Conference
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• 2000.05a
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• pp.151-152
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• 2000

During in door or outdoor mass culture, Porphyra have been easily contaminated with bacteria, protozoa and microalgal species. Several axenic treatments for Porphyra thalli have been published (Polne-Fuller and Gibo 1984; Chen and McCracken 1993), but axenic techniques for neutral spores and conchocelis we not developed. In this work we describe the procedure for axenic isolation of neutral spores and conchocelis of Porphyra yezoensis (omitted)

• ALGAE
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• v.18 no.1
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• pp.21-28
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• 2003

• ALGAE
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• v.26 no.1
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• pp.79-86
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• 2011

Physiological studies on the hybrid by crossing between two dioecious species, Porphyra pseudolinearis and P. dentata from Korea were conducted at constant temperatures (5, 10, 15, 20, and $25^{\circ}C$), at photon flux densities (10, 20, 40, and $80\;{\mu}mol\;m^{-2}s^{-1}$) under photoperiods (14 L : 10 D and 10 L : 14 D). In the hybrid, higher growth of conchocelis was observed at 20 and $40\;{\mu}mol\;m^{-2}s^{-1}$ under 14 L : 10 D. Conchosporangial branches were produced under $10-80\;{\mu}mol\;m^{-2}s^{-1}$ at only $25^{\circ}C$, and were abundant when the conchocelis was cultured under 10 L : 14 D. Foliose thalli of the hybrid grew well at the conditions of $10-20^{\circ}C$, 10 L : 14 D and $15-20^{\circ}C$, 14 L : 10 D. The foliose thalli grew very slowly at $5^{\circ}C$ and $30^{\circ}C$, respectively. No archeospores were observed at any culture conditions. Spermatangial and zygotosporangial sori were formed at the marginal portion of mature thallus. Zygotospores from the hybrid were released at $10-2^{\circ}C$ under both photoperiods, and gave rise to form conchocelis filament. Monoecious thalli were observed at $10^{\circ}C$ under 14 L : 10 D. Neither monospores nor protothalli were produced from the conchocelis in culture.

• Journal of Aquaculture
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• v.13 no.4
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• pp.353-357
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• 2000

Porphyra pseudolinearis and P. dentata from Korea were crossed and the hybrid was cultured at different temperatures (5, 10, 15, 20 or $25^{\circ}C$), photon flux densities (10, 20, 40 or 80${\mu}$mol m$^{-2}$s$^{-1}$) under photoperiods (14L:10D and 10L:14D). In the hybrids, the conchocelis grew faster at 2$0^{\circ}C$ and 40$\mu$mol m$^{-2}$ s$^{-1}$ at $25^{\circ}C$ only, and were abundant, when cultured under 10L:14D. Foliose thalli of the hybrid grew rapidly at conditions of 10-2$0^{\circ}C$, 10L:14D and 15-2$0^{\circ}C$, 14L:10D but slowly at 5 and 2$0^{\circ}C$. No archeospores were observed any tested culture condition. Spermatangial and zygotosporangial sori were formed at the marginal portion o mature thallus. Zygotospores from the hybrid were released at 10-2$0^{\circ}C$ under both photoperiods, and gave rise to form conchocelis filament. Monoecious thalli were observed at 1$0^{\circ}C$ under 14L:10D. Neither monospores nor protothalli were produced from the conchocelis in culture.

• Proceedings of the Korean Aquaculture Society Conference
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• 2005.05a
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• pp.88-89
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• 2005

• Korean Journal of Fisheries and Aquatic Sciences
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• v.9 no.1
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• pp.56-60
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• 1976

In order to find effective seed collection method from cultivated Porphyra, benthic diatom elimination, culturing conditions of Conchocelis, liberation of conchospores and treatment of the fronds to obtain monospores have been studied. Elimination of benthic diatoms from Porphyra fronds is successfully performed by careful brushing the fronds in sea water and freshwater alternatively. For the culturing of Conchocelis Schleiber's solution enriched with only soil extracts, vitamins and Fe-EDTA was satisfactory. Growth under 16 hours illumination is 1.29 times faster than those under 10 hours illumination. When the culturing water was airated the growth was $1.41\~l.50$ times faster than the growth in stagnant water. Total amount of conchospores liberated from Conchocelis which has been cultured under airation was much more than those of conchospores under stagnant condition. Effective liberation of monospores was observed in the fronds which have been dried in air for 6 hours $(21.23\~24.19\%\;water\;content)$.

• Korean Journal of Environment and Ecology
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• v.31 no.1
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• pp.88-92
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• 2017

This study investigated the growth and maturation of free conchocelis of Pyropia yezoensis under monochromatic light of blue light(480 nm), green light(550 nm), yellow light(600 nm) and red light(730 nm) which measured the colony diameter, content of chlorophyll a and conchosporangium formation. In the result, the most of colony diameter and chlorophyll a showed under the blue light, $2,472.6{\pm}27.0{\mu}m$ and $1.55{\pm}0.03mg\;g.dw^{-1}$. Also, the chlrophyll a content of conchocelis was under the blue light. Therefore, the blue light might be favorable for the growth and maturation of conchocelis of P. yezoensis. This study will lead development of indoor culture technologies.

• ALGAE
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• v.19 no.4
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• pp.303-309
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• 2004

The laboratory culture study of Porphyra seriata Kjellman from Korea was conducted at different conditions of temperatures (5, 10, 15, 20, 25 and 30${^{\circ}C}$), photon flux densities (10, 20, 40 and 80 $\mu$mol $^{-2}s^{-1}$) and photoperiods (14L: 10D and 10L:14D). Conchocelis filaments grew fast at 15-20${^{\circ}C}$ and 20-80 $\mu$mol $^{-2}s^{-1}$ under both photoperiods. Concho sporangial branches were produced at 5-25${^{\circ}C}$, and abundant when the conchocelis filaments were cultured at higher temperatures of 20-25${^{\circ}C}$ under both photoperiods. Foliose thalli grew well at 15-20${^{\circ}C}$ under 10L:14D and at 20${^{\circ}C}$ under 14L:10D. At 30${^{\circ}C}$, the foliose thallus failed to survive. No archespores were observed at any culture conditions. Spermatangia and zygotosporangia were formed in squarish patches at the upper marginal portion of mature thalli. Anatomical examination revealed that the mature spermatangia were 64 (a/4, b/2, c/8) and 128 (a/4, b/4, c/8), and that of zygotosporangium was 16 (a/2, b/2, c/4) according to the Hus' formula.

• ALGAE
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• v.21 no.2
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• pp.193-208
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• 2006

Porphyra pulchella sp. nov. Ackland, West, Scott and Zuccarello was obtained at Mimosa Rock National Park, New South Wales; Westgate Bridge, Victoria, Australia; and Waihau Bay, North Island, New Zealand. It occurs mainly in mangrove habitats and is very small (± 1 mm) in field collections. In laboratory culture at 21 ± 2°C tiny blades (0.5-3.0 mm) reproduced exclusively by archeospores liberated from vegetative cells of the upper sector of the blades. The archeospores displayed amoeboid and gliding motility once discharged. At 14 ± 2°C the blades grew to 25 mm and produced longitudinal spermatangial streaks mixed with ‘phyllosporangial’ streaks. The discharged ‘phyllospores’ showed amoeboid motility and germinated forming asexual blades. A conchocelis phase with typical bangiophycidean pit connections was observed in blade cultures after 8-10 weeks at 14 ± 2°C. Conchocelis filaments produced conchosporangia and these released amoeboid conchospores that developed into archeosporangiate blades. Molecular data indicate that all 3 isolates are genetically identical.