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Polymorphisms in Epigenetic and Meat Quality Related Genes in Fourteen Cattle Breeds and Association with Beef Quality and Carcass Traits

  • Liu, Xuan;Usman, Tahir;Wang, Yachun;Wang, Zezhao;Xu, Xianzhou;Wu, Meng;Zhang, Yi;Zhang, Xu;Li, Qiang;Liu, Lin;Shi, Wanhai;Qin, Chunhua;Geng, Fanjun;Wang, Congyong;Tan, Rui;Huang, Xixia;Liu, Airong;Wu, Hongjun;Tan, Shixin;Yu, Ying
    • Asian-Australasian Journal of Animal Sciences
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    • v.28 no.4
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    • pp.467-475
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    • 2015
  • Improvement for carcass traits related to beef quality is the key concern in beef production. Recent reports found that epigenetics mediates the interaction of individuals with environment and nutrition. The present study was designed to analyze the genetic effect of single nucleotide polymorphisms (SNPs) in seven epigenetic-related genes (DNMT1, DNMT3a, DNMT3b, DNMT3L, Ago1, Ago2, and HDAC5) and two meat quality candidate genes (CAPN1 and PRKAG3) on fourteen carcass traits related to beef quality in a Snow Dragon beef population, and also to identify SNPs in a total of fourteen cattle populations. Sixteen SNPs were identified and genotyped in 383 individuals sampled from the 14 cattle breeds, which included 147 samples from the Snow Dragon beef population. Data analysis showed significant association of 8 SNPs within 4 genes related to carcass and/or meat quality traits in the beef populations. SNP1 (13154420A>G) in exon 17 of DNMT1 was significantly associated with rib-eye width and lean meat color score (p<0.05). A novel SNP (SNP4, 76198537A>G) of DNMT3a was significantly associated with six beef quality traits. Those individuals with the wild-type genotype AA of DNMT3a showed an increase in carcass weight, chilled carcass weight, flank thicknesses, chuck short rib thickness, chuck short rib score and in chuck flap weight in contrast to the GG genotype. Five out of six SNPs in DNMT3b gene were significantly associated with three beef quality traits. SNP15 (45219258C>T) in CAPN1 was significantly associated with chuck short rib thickness and lean meat color score (p<0.05). The significant effect of SNP15 on lean meat color score individually and in combination with each of other 14 SNPs qualify this SNP to be used as potential marker for improving the trait. In addition, the frequencies of most wild-type alleles were higher than those of the mutant alleles in the native and foreign cattle breeds. Seven SNPs were identified in the epigenetic-related genes. The SNP15 in CAPN1 could be used as a powerful genetic marker in selection programs for beef quality improvement in the Snow Dragon Beef population.

An Analysis of Elements in Yen-Ben Street That Form a Sense of Place as an Ethnic Enclave (소수민족집단체류지역(Ethnic Enclave)으로서의 옌볜거리의 장소성 형성 요인 분석)

  • Han, Sung-Mi;Im, Seung-Bin
    • Journal of the Korean Institute of Landscape Architecture
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    • v.36 no.6
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    • pp.81-90
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    • 2009
  • This study seeks those elements that form a sense of place in Yen-Ben Street, which represents a typical ethnic enclave in Seoul, to provide a basic resource in the creation of an urban landscape that can provide a positive space for cultural diversity. The results of the study can be summarized as follows: First, the element of a physical environment that develops a sense of place was in fact the poor dwellings that correspond to the economic condition of Korean Chinese. While this element has a negative cognition to outsiders, Korean Chinese feel positively toward it. Secondly, signboards were a physical element of sense of place which retains cultural identity as a means of communication inside the community. Thirdly, it was found that activities such as shopping, recreation, and the exchange of information that are found in the pursuit of daily life act as an essential element in the formation of a sense of place even more than architectural elements. Fourthly, the appropriation of space by Korean Chinese and the isolation from the surroundings were obvious. This isolation is perceived as a negative sense of place formation to outsiders in Yen-Ben Street. Fifthly, the aspects of cultural dualism, mingling the concepts of home country, language, writing, and food have also affected the formation of a sense of place in the area. Sixthly, transience was a prominent phenomenon of Yen-Ben Street and is strengthened by illegal immigration. Although transience causes negative impacts such as in a lack of concern for the residential environment, it acts as a positive factor in the sense of place by mitigating uneasiness, and strengthening insider ties and cooperation.

Application of Liriope platyphylla, Ornamental Korean Native Plants, for Contaminated Soils in Urban Areas (도시 내 중금속 오염지의 관상식물로서 자생 맥문동(Liriope platyphylla)의 적용성 평가)

  • Ju, Jin-Hee;Yoon, Yong-Han
    • Journal of the Korean Institute of Landscape Architecture
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    • v.42 no.5
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    • pp.81-87
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    • 2014
  • Heavy metal pollution is a widespread global problem causing serious environmental concern. Heavy metals such as Cd, Pb, and Zn can induce toxicity in all organisms if the soil levels of contaminants reach critical values. The aim of the present study was to examine the application of Liriope platyphylla, an ornamental Korean native plant with great potential for contaminated soil in urban areas, to determine tolerance for Cd, Pb, and Zn. Plants were grown in amended artificial soil with Cd, Pb, and Zn at 0, 100, 250, and $500mg{\cdot}kg^{-1}$ for 7 months. The length of leaf, width of leaf, total leaf number, dead leaf number, new leaf number, chlorophyll contents, and ornamental value were monitored from May to August, during growth the period. The relative leaf length and leaf width displayed rapidly decreasing tendencies with an increasing Cd concentration beginning from 4 months after planting. The same decreasing tendency was observed in total leaf number, new leaf number, chlorophyll contents, and ornamental values showed a trend of Control> $Cd_{100}$ > $Cd_{250}$ > $Cd_{500}$. In Pb concentration treatments, the relative leaf length and leaf width were significantly lower in plants grown at $250mg{\cdot}kg^{-1}$ and $500mg{\cdot}kg^{-1}$ as compared to the Control, $100mg{\cdot}kg^{-1}$. The total leaf number, new leaf number, and dead leaf number did not show significant difference among treatments in Control and $Pb_{100}$ but chlorophyll contents and ornamental value decreased with increasing Pb supply concentration treatments. However, in Zn supply treatments, the relative leaf length was higher at $100mg{\cdot}kg^{-1}$ than the Control, $250mg{\cdot}kg^{-1}$, $500mg{\cdot}kg^{-1}$, but the relative leaf width decreased compared to the Control, $Zn_{100}$, $Zn_{250}$, and $Zn_{500}$. The total leaf number, dead leaf number, new leaf number, and ornamental value showed the lowest value in plants grown in $Zn_{500}$ treatment but no significant differences were found among other treatments.

Growth and Morphological Charactersistics of Soybean Sprouts Treated with Leaf Extracts of Thea sinensis L. and Eucommia ulmoides Oliver (녹차와 두충 잎 추출물 처리에 따른 콩나물의 생장 및 형태적 특징)

  • Kang, Jin-Ho;Park, Cheol-Jong;Yoon, Soo-Young;Jeon, Seung-Ho;Her, Chang-Young
    • Korean Journal of Medicinal Crop Science
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    • v.13 no.1
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    • pp.11-16
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    • 2005
  • Soybean sprouts presoaked to leaf extracts were on sale as taking a growing concern about wellbeing foods. The study was carried out to compare the effects of culture period after presoaking tea (Thea sinensis L.) and hard rubber tree (Eucommia ulmoides Oliver) leaf extracts on lateral root formation, growth and morphological characters of the sprouts. Seeds of three cultivars, Pungsannamulkong, Sowonkong and Junjery, were imbibed for 5 hours into 10% solutions immediately before 5, 6 and 7 day cultures. The cultured sprouts were classified into 4 categories to calculate their composition rates on the base of hypocotyllengths; >7 cm (A),4 to 7 cm (B),< 4 cm (C) and not germinated (D), and their morphological characters, fresh and dry weights were measured. Rate of A was the lowest in Junjery of the three cultivars, while that of C showed reverse result compared to A. Similar results to that of A were observed between the three cultivars in sprout length including hypocotyl and root, diameters in middle and hook parts of hypocotyl, total fresh and dry weights. Regardless of tea and hard rubber tree leaf extract treatments, longer culture periods increased rate of A due to decrements of B, C and D, and also did the growth and the morphological characters mentioned above. Although longer culture periods reduced the increment of lateral roots, the latter treatment was more delayed in their formation and less in the roots per sprout than the former one. The growth and the morphological characters above were less in the latter treatment than in the former one in general, meaning that hard rubber tree leaf extract could more repress growth of soybean sprouts than tea leaf extract.

The Joseon Confucian Ruling Class's Records and Visual Media of Suryukjae (Water and Land Ceremony) during the Fifteenth and Seventeenth Centuries (조선 15~17세기 수륙재(水陸齋)에 대한 유신(儒臣)의 기록과 시각 매체)

  • Jeong, Myounghee
    • Korean Journal of Heritage: History & Science
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    • v.53 no.1
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    • pp.184-203
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    • 2020
  • The Confucian ruling class of the Joseon Dynasty regarded Buddhist rituals as "dangerous festivals." However, these Buddhist ceremonies facilitated transitions between phases of life from birth till death and strengthened communal unity through their joint practice of the rites. Ritual spaces were decorated with various utensils and objects that transformed them into wondrous arenas. Of these ornaments, Buddhist paintings served as the most effective visual medium for educating the common people. As an example, a painting of the Ten Kings of the Underworld (siwangdo) could be hung as a means to illustrate the Buddhist view of the afterlife, embedded in images not only inside a Buddhist temple hall, but in any space where a Buddhist ritual was being held. Demand for Buddhist paintings rose considerably with their use in ritual spaces. Nectar ritual paintings (gamnodo), including scenes of appeasement rites for the souls of the deceased, emphasized depictions of royal family members and their royal relatives. In Chinese paintings of the water and land ceremony (suryukjae), these figures referred to one of several sacred groups who invited deities to a ritual. However, in Korean paintings of a nectar ritual, the iconography symbolized the patronage of the royal court and underlined the historicity and tradition of nationally conducted water and land ceremonies. This royal patronage implied the social and governmental sanction of Buddhist rituals. By including depictions of royal family members and their royal relatives, Joseon Buddhist paintings highlighted this approval. The Joseon ruling class outwardly feared that Buddhist rituals might undermine observance of Confucian proprieties and lead to a corruption of public morals, since monks and laymen, men and women, and people of all ranks mingled within the ritual spaces. The concern of the ruling class was also closely related to the nature of festivals, which involved deviation from the routines of daily life and violation of taboos. Since visual media such as paintings were considered to hold a special power, some members of the ruling class attempted to exploit this power, while others were apprehensive of the risks they entailed. According to Joseon wangjo sillok (The Annals of the Joseon Dynasty), the Joseon royal court burned Buddhist paintings and ordered the arrest of those who created them, while emphasizing their dangers. It further announced that so many citizens were gathering in Buddhist ritual spaces that the capital city was being left vacant. However, this record also paradoxically suggests that Buddhist rituals were widely considered festivals that people should participate in. Buddhist rituals could not be easily suppressed since they performed important religious functions reflecting the phases of the human life cycle, and had no available Confucian replacements. Their festive nature, unifying communities, expanded significantly at the time. The nectar ritual paintings of the late Joseon period realistically delineated nectar rituals and depicted the troops of traveling actors and performers that began to emerge during the seventeenth century. Such Buddhist rituals for consoling souls who encountered an unfortunate death were held annually and evolved into festivals during which the Joseon people relieved their everyday fatigue and refreshed themselves. The process of adopting Buddhist rituals-regarded as "dangerous festivals" due to political suppression of Buddhism in the Confucian nation-as seasonal customs and communal feasts is well reflected in the changes made in Buddhist paintings.

A study of serotyping of Streptococcus pneumoniae by multibead assay (다중구슬 분석법에 의한 폐구균 혈청형 결정 연구)

  • Cho, Ky Young;Lee, Jung Ah;Cho, Sung Eun;Kim, Nam Hee;Lee, Jin A;Hong, Ki Sook;Lee, Hoan Jong;Kim, Kyung Hyo
    • Clinical and Experimental Pediatrics
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    • v.50 no.2
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    • pp.151-156
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    • 2007
  • Purpose : Streptococcus pneumoniae is a major etiologic agent for pneumonia, meningitis, otitis media, and sepsis among young children. Multi-drug resistant strains have raised great concern worldwide, thus the importance of prevention with vaccines has been emphasized. However, vaccines may force the appearance of pneumococcal infections by nonvaccine serotypes. Thus, distribution of pneumococcal serotypes should be monitored to estimate vaccine efficacy. We used a new and efficient multibead assay in determining pnemococcal serotypes. Methods : From January to February 2005, 643 children were recruited from ten day care centers to isolate pneumococci from their oropharynx. Pneumococcal serotyping was performed on 62 pneumococcal isolates from 60 children by multibead assay. This immunoassay required two sets of latex particles coated with pneumococcal polysaccharides and serotype-specific antibodies. Twenty four newly developed monoclonal antibodies specific for common serotypes and a pool of polyclonal rabbit sera for some of the less common serotypes were used. Results : The most prevalent pneumococcal serotypes were serotype 6A, 19A, 19F, 23F, and 11A/D/F which accounted more than 50 precent of all the 62 pneumococcal isolates. We found that multibead assay can be performed very rapidly and objectively. Conclusion : This multibead immunoassay was very useful in serotyping clinical isolates of S. pneumoniae because it was simple, reliable and fast.

The Pattern of Pusan Station Shopping District( I ) (부산역(釜山驛) 상점가(商店街)의 패턴( I ))

  • Kim, Won-Kyung
    • Journal of the Korean association of regional geographers
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    • v.5 no.1
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    • pp.25-75
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    • 1999
  • This study concern with the pattern of Pusan Station Shopping District within Pusan City, Korea, one of the third ranked centers within Pusan-the first ranked is CBD, the second is subCBD, the third are Spa Shopping district, Tongnae shopping district, Jobang-Ap shopping district and Yangjeong shopping district. This paper serves as a part of series studies which attempt to clarify the internal structure of Pusan as a whole. Part(I) of this study deals with the pattern of functions including information & culture, business, recreation and retailing. The results are as follows. 1. Pusan Station is one of higher ranked class of central places within Pusan City. But the ratio of dwelling houses is the highest in Pusan Station among the 9 higher ranked central places within Pusan. It means that the residential areas still remain along the maze type of narrower streets inner part of the blocks, especially western belt of this shopping district. 2. Business functions are one of the important criteria which classified the central places within the city and Pusan Station is the first ranked shopping districts in terms of these criteria among the 9 higher ranked central places including CBD within Pusan City. It suggests that Pusan Station is the miniature of the CBD, SubCBD is not the miniature of CBD in the view point of business function. It also means that Pusan Station Shopping District has been developed as a special shopping district or as a special central place. It has not been evolved as a center of the hierarchy of central places within the city by the natural or organic growth. 3. Pusan Station as 'the third space or drinking place' and it's characteristics as a amusement center presents at so called the 'Texas Town'-free tax area- whose customers are mainly U. S. soldiers and foreign sailors. But from the 1990s, this 'Town' changed to 'Russian Town', You can easy to meet many Russians and Russian lettered signboards-not English- along the street. 4. Convenient stores distribute mainly at back streets or along the narrower streets. It means that this kinds of stores have a residential oriented character. And a certain convenient stores locate with almost same distances. It suggests that it's hinterland is limited in nearer neighborhoods.

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Changes of Street Patterns in Central Part of Taegu City (大邱市 都心部의 街路網 變化)

  • Choi, Seok-Joo
    • Journal of the Korean Geographical Society
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    • v.31 no.3
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    • pp.593-612
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    • 1996
  • This study concern with the changes of street from the Choseon Dynasty to present days around Old-Boundary in Taegu, analyzing the backgrounds of change factors and development trends of the Taegu City. The basis element of a city structure is the street. Therefore, in this study, a chage of street space of a city was investigted. Historically, Taegu was a walled city and had a Mono-nucleus which was restricted by the castle, and served as a starting point of formation of spatial structuure. The form of the artery street took a "T" pattern, othe streets were formed in irregular shapes. As the city grew gradually, the castle was removed on account of diversification in traffic network, change of socio-economic organization in traffic network, change of socio-economic organization, formation of industrial bases and functional distribution. CBD of this city has been located within the area surrounded by these streets. This is a kind of general pattern of traditional walled cities through the world in both Western and Oriental societies. A s the begining of this centry, a 'Dark Ages' descended upon Korea because the country was under the Japanese-Korean Annexation, and, throughout this period, the urban planning was planned exclusively for Japanese. The street pattern within residential areas of Korea took the maze type, in contrast with Japanese residential areas which showed grid pattern of streets. This is another general pattern of almost of all colonial cities especially in Asia. High class residential areas were planned and built by Japanese, and they were located within 5-10 minutes' on-foot distance from the CBD hard core. This high prestige has continued until the 1980s when it occurred land use succession which commerical functions invaded into residential areas. Back in the colonial period, there was a between two hetrogeneous groups due to the fact that the Japanese lived mainly oriented the new railway system but that Koreans still lived along the old highway system which ran through the Korea Peninsula. Street netwook formed in the above process has maintained its shape without great changes after the liberation form the Japanese Colony. Taegu has, accordingly, developed ring-radial network system which has been a combination of radial and ring facilities. The present conditions of street patterns in Taegy mainly depend on 4 rings and 8 radius, with grid pattern street able to be found in Old Boundary.

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Study on the Consequence Effect Analysis & Process Hazard Review at Gas Release from Hydrogen Fluoride Storage Tank (최근 불산 저장탱크에서의 가스 누출시 공정위험 및 결과영향 분석)

  • Ko, JaeSun
    • Journal of the Society of Disaster Information
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    • v.9 no.4
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    • pp.449-461
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    • 2013
  • As the hydrofluoric acid leak in Gumi-si, Gyeongsangbuk-do or hydrochloric acid leak in Ulsan, Gyeongsangnam-do demonstrated, chemical related accidents are mostly caused by large amounts of volatile toxic substances leaking due to the damages of storage tank or pipe lines of transporter. Safety assessment is the most important concern because such toxic material accidents cause human and material damages to the environment and atmosphere of the surrounding area. Therefore, in this study, a hydrofluoric acid leaked from a storage tank was selected as the study example to simulate the leaked substance diffusing into the atmosphere and result analysis was performed through the numerical Analysis and diffusion simulation of ALOHA(Areal Location of Hazardous Atmospheres). the results of a qualitative evaluation of HAZOP (Hazard Operability)was looked at to find that the flange leak, operation delay due to leakage of the valve and the hose, and toxic gas leak were danger factors. Possibility of fire from temperature, pressure and corrosion, nitrogen supply overpressure and toxic leak from internal corrosion of tank or pipe joints were also found to be high. ALOHA resulting effects were a little different depending on the input data of Dense Gas Model, however, the wind direction and speed, rather than atmospheric stability, played bigger role. Higher wind speed affected the diffusion of contaminant. In term of the diffusion concentration, both liquid and gas leaks resulted in almost the same $LC_{50}$ and ALOHA AEGL-3(Acute Exposure Guidline Level) values. Each scenarios showed almost identical results in ALOHA model. Therefore, a buffer distance of toxic gas can be determined by comparing the numerical analysis and the diffusion concentration to the IDLH(Immediately Dangerous to Life and Health). Such study will help perform the risk assessment of toxic leak more efficiently and be utilized in establishing community emergency response system properly.

Study on verification of various national standards regarding phthalate testing in industrial products (공산품 중 프탈레이트류 함유량 분석법에 관한 국내외 표준의 검증 연구)

  • Song, Moon-Hwan;Cho, Young-Dal;Choe, Eun-Kyung;Myoung, Young-Chan
    • Analytical Science and Technology
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    • v.25 no.3
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    • pp.178-189
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    • 2012
  • As phthalates classified as toxic to reproduction category 2 and endocrine disrupting chemicals were more strictly regulated as Substances of Very High Concern (SVHC) for authorization in under EU REACH and considered as priority substances in RoHS II, standardization of phthalate testing method is now being proposed in IEC 62321 of IEC TC 111 and the 2nd revision of KS M 1991 is also finished. In order to assist standardization activities related to phthalating testing, solvent extraction part of existing national standards were compared and verified. Recovery of DEHP (diethylhexyl phthalate) from PVC (polyvinyl chloride) by Soxhlet extraction increased in the order of methanol, toluene, dichloromethane and hexane from 46.9% to 95.3% as measured by GC-MS. Optimum extraction time was verified to be 6 hours using hexane. Recovery of DBP (dibutyl phthalate), BBP (butylbenzyl phthalate), and DEHP from different matrixes such as PVC, nitro cellulose, ABS (acrylonitrile butadiene styrene). and EPDM(ethylene propylene diene monomer) rubber were evaluated to be more than 90% up to 99%. The detection limits of phthalates in solvent extraction followed by GC-MS analysis were 0.08~0.3 ${\mu}g/mL$ in solution and 8~30 mg/Kg in polymeric samples. GC-MS analyses of phthalates were carried out using different solvent extraction based on the EN 14372, ASTM D 7083, Japanese test method (MHLW 0906-4) and KS M 1991, proving that equivalent recoveries ranging from 98%~99% were obtained. DBP and DEHP were detected in three consumer products such as a child toy, a power cable and manicure with the amount of 22~1,910 mg/kg.