• Journal of Veterinary Science
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• v.24 no.5
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• pp.70.1-70.14
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• 2023

Background: Mycobacterium avium subspecies paratuberculosis (MAP) causes a chronic and progressive granulomatous enteritis and economic losses in dairy cattle in subclinical stages. Subclinical infection in cattle can be detected using serum MAP antibody enzyme-linked immunosorbent assay (ELISA) and fecal polymerase chain reaction (PCR) tests. Objectives: To investigate the differences in blood parameters, according to the detection of MAP using serum antibody ELISA and fecal PCR tests. Methods: We divided 33 subclinically infected adult cattle into three groups: seronegative and fecal-positive (SNFP, n = 5), seropositive and fecal-negative (SPFN, n = 10), and seropositive and fecal-positive (SPFP, n = 18). Hematological and serum biochemical analyses were performed. Results: Although the cows were clinically healthy without any manifestations, the SNFP and SPFP groups had higher platelet counts, mean platelet volumes, plateletcrit, lactate dehydrogenase levels, lactate levels, and calcium levels but lower mean corpuscular volume concentration than the SPFN group (p < 0.017). The red blood cell count, hematocrit, monocyte count, glucose level, and calprotectin level were different according to the detection method (p < 0.05). The SNFP and SPFP groups had higher red blood cell counts, hematocrit and calprotectin levels, but lower monocyte counts and glucose levels than the SPFN group, although there were no significant differences (p > 0.017). Conclusions: The cows with fecal-positive MAP status had different blood parameters from those with fecal-negative MAP status, although they were subclinically infected. These findings provide new insights into understanding the mechanism of MAP infection in subclinically infected cattle.

• Journal of Korean Society for Geospatial Information Science
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• v.4 no.2 s.8
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• pp.35-45
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• 1996

The reflectance of chlorophyll was measured using UV-VIS spectrophotometer with the reflectance integrator in the laboratory in order to define its spectral characteristics. Sharp peaks appear at around 485nm and 655nm due to fluorescence and scattering, and the reflectance of chlorophyll increases at 580nm. With the increase in the chlorophyll concentration, the reflectance also increases. We have applied TM data to the reflectance spectrum of chlorophyll and have developed two formula with which one can estimate the chlorophyll concetration. Satellite re sensing, with its synoptic overage, is used to obtain the chlorophyll concentration in Daecheong reservoir. The approach involved acquisition of water quality samples front boat simultaneous with Landsat 5 satellite overpass. The remotely-sensed data and the ground truth data were obtained oil 20 June 1995 and on 18 March 1996. Regression models have been developed between the chlorophyll concentration and Landsat Thematic Mapper digital data. As the regression model was determined based on the correlation coefficient which was higher than 0.7 and the spectral characteristics of chlorophyll, and we have applied it to the entire study area to genelate a distribution map of trophic state. According to the trophic state map made based upon Aizaki's TSI and chlorophyll a concentration, the area where Okchun stream was flowing into was shown to be polluted the most all over the Daechung reservoir by showing an eutrophic state in June 1995 and a mesotrophic state in March 1996.

• Journal of Wetlands Research
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• v.5 no.2
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• pp.57-65
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• 2003

This study was corried out to provide basic data for water analysis biodiversity, Actual vegetation map, flora and fauna on the Sanchon-tideland(Geojaesi), Doma-tideland(Namhaegun) in Kyungsangnamdo from august 2002 to February 2003. This tidelands was very broad and was being reclaimed. Therefore, it was anxious about destroying the habitat of the water birds. Since the water temperature was influenced by the surrounding air, it was highest in summer and lowest in winter. pH value and DO concentration of the water was highest in winter in the Sanchon-tideland and in surmmer in the Doma-tideland. COD concentration was lowest in winter in two tidelands. SS concentration was highest in summer. T-P and T-N concentration were very low but decreased with the seasonal change from summer to winter. Vascular plants of this area consist of 11 varieties, 67 species, 64 genera, 29 families(Sanchon-tideland) and 1 forma, 11varieties, 62 species, 64 genera, 30 families(Doma-tideland). The predominant community of this area, Phragmites communis Community were found on the seashore. The total insect specimens were classified into 120 species, 48 families, 10 orders(Sanchon-tideland) and perdominant species were Gabala argentata(Dominance 18.78%, Diversity index 1.75, Richness index 45.78). And 91 species, 40 families, 8 orders(Doma-tideland) and perdominant species were Sternolophus (Sternolophus) rufipes(Dominance 15.47%, Diversity index 1.47, Richness index 33.66). The total Fishes were classified into 3 species, 2 families, 1 order and Amphibia were 3 species, 2 families, 1 order. And Salicomia herbacea should be worthwhile species to conserve in this area. Because the water birds lived in The Tideland Sanchon and Doma, it was considered that it was necessary to preserve them.

• KOREAN JOURNAL OF PACKAGING SCIENCE & TECHNOLOGY
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• v.28 no.1
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• pp.9-14
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• 2022

Packages of different atmospheres (air (control), 100% CO₂, vacuum, and vacuum + O₂ absorber) were prepared for 0.4 kg rice cake (ddukgukdduk) using gas-barrier plastic film and stored at 10℃ for 11 days. The stored products were evaluated in their packages atmosphere, total aerobic bacteria, yeast and molds, texture and sensory quality during storage period. In the air package, the O₂ concentration decreased from initial 21% to 16% on storage 4 days and the CO₂ concentration increased to 23% on storage of 11 days, which resulted from the growth of microorganisms. CO₂ concentration decreased from initial 98% to 36% on storage 11 days in the 100% CO₂ package. It is reasoned that CO₂ was dissolved into the product reducing the volume of the package. Vacuum and vacuum +O₂ absorber package maintained shrunk vacuum condition until 11 days of storage. Total aerobic bacteria count increased significantly in the control package (6.41 log (cfu/g) after 11 days) compared to the 100% CO₂ package (4.96 log (cfu/g) after 11 days). Yeast and molds were 6.66 in control package, 3.43 in 100% CO₂ package, 4.66 in vacuum package, and 3.78 in vacuum + O₂ absorber package after 11 days. There was no significant difference between control and the other treatments for the texture of the stored products. Sensory quality was the worst in control package on the storage of 8 days. All treatment groups except control improved the quality preservation, but vacuum and vacuum + O₂ absorber packages suffered from cracking of the product. Thus 100% CO₂ flushing is suggested as a desired packaging condition.

• Proceedings of the Korea Air Pollution Research Association Conference
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• 1999.04a
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• pp.50-51
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• 1999

• Journal of Animal Science and Technology
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• v.51 no.4
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• pp.337-342
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• 2009

The feasibility of reutilization of magnesium ammonium phosphate (MAP) or struvite slurry recovered from the process through microwave irradiation was studied in this experiment. For this purpose, 4 different operations were performed with or without Mg source addition and different levels of MAP recycled in a batch reactor. Dissolution rate of MAP, ${NH_4}^+$ elimination pattern and physicochemical changes of MAP during microwave irradiation were also studied. The result showed that only 33% orthophosphate ($PO_4-P$) and 27% $NH_4-N$ removal occurred without adding any external Mg source (run A), whereas 87% $PO_4-P$ and 40% $NH_4-N$ removed when 1.0 M ratio of $MgCl_2$ (run B) was added based on $PO_4-P$ in influent. Although the addition of 1.0 molar ratio of microwave irradiated MAP (Run C) removed lower $PO_4-P$ and $NH_4-N$ than 1.0 M $MgCl_2$ (run B), $PO_4-P$ removal was double when compared with no Mg addition (run A). Addition of half MAP and half $MgCl_2$ (run D) showed the similar removal efficiency (88% $PO_4-P$ and 35% $NH_4-N$) with sole $MgCl_2$ addition (run B). Based on these results, the reutilization of MAP irradiated by microwave would be a feasible way to enhance the removal efficiencies of N and P, as well as curtail the Mg chemical usage. Track study showed that $NH_4-N$ gradually increased at initial stage of microwave irradiation of MAP, and then started eliminating from liquor as temperature increased over $45^{\circ}C$. Dissolution rate of ${PO_4}^{-3}$ during microwave irradiation was proportional to the initial MAP concentration, having $0.0091x^{0.6373}$ mg/sec. It was found from the scanning electron microscope (SEM) study that physical structure of MAP crystal started breaking down into small cube granules within very short time by electromagnetic vibration force during microwave irradiation and then gradually melted down into solution.

• 한국연소학회:학술대회논문집
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• 2014.11a
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• pp.379-381
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• 2014

Influence of fuel concentration gradient was investigated near flame extinction limit in buoyancy-suppressed non-premixed counterflow flame with triple co-flow burner. The use of He curtain flow produced a microgravity level of $10^{-2}-10^{-3}g$ in He-diluted non-premixed counter triple co-flow flame experiments. Flame stability map was presented based on flame extinction and oscillation near extinction limit. The stability map via critical diluent mole fraction with global strain rate was represented by varying outer and inner He-diluted mole fractions. The flame extinction modes could be classified into five: an extinction through the shrinkage of the outmost edge flame forward the flame center with and without self-excitation, respectively ((I) and (II)), an extinction via the rapid expansion of a flame hole while the outmost edge flame is stationary (III), both the outermost and the center edge flames oscillate, and then a donut shaped flame is formed or the flame is entirely extinguished (IV), a shrinkage of the outermost edge flame without self-excitation followed by shrinking or sustain the inner flame (V).

• Journal of Environmental Science International
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• v.11 no.10
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• pp.1081-1087
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• 2002

This study was performed to research the characteristics of suspended particulate for Yellow Sand of January, 1999 in Busan. Yellow Sand frequency during 13 years(1988~2000) in Busan showed maximum in April(57%), next to March(21%), May(16%). According to result of 850hPa weather map and backward isentropic trajectory, this event originated from the Gobi Desert and the Loess Plateau of China. And three mode was found in time series of TSP and PM10 concentration, primary peak showed the maximum hourly concentration at ali station. Gamjeondong as industrial site showed the highest TSP concentration and also had the longest high concentration($geq700\mu\textrm{g}/m^3$). In PM10, concentration of primary peak showed maximum value at Yeonsandong, maximum concentration of secondary and third peak was Deokcheondong. Lasted time from primary peak to secondary peak was about 30 hours, between secondary peak and third peak was 18 hours in Busan, The traveling time between occurrence of Yellow Sand the finding of it was 8~9 hours in Busan and 4~5 hours in central area.

• Food Science of Animal Resources
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• v.31 no.5
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• pp.710-718
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• 2011

The effects of organic acids mix (0.4%) and modified atmosphere packaging (MAP) on the storage quality of sliced bacon were investigated. Pork bellies were treated with or without organic acids at the curing stage. The organic acids mix comprised 35% sodium acetate, 25% salt, 15% calcium lactate, 11% trisodium citrate, 7% ascorbate, and 7% citric acid. The cured pork bellies were smoked and packaged with 50% $CO_2$ + 50% $N_2$ (50% $CO_2$-MAP) and 100% $N_2$ (100% $N_2$-MAP), and stored at $5^{\circ}C$ for 14 d. The 50% $CO_2$-MAP showed a higher pH value (p<0.05) up to 10 d, a lower protein deterioration (p<0.05) as measured by volatile basic nitrogen (VBN) from 6 to 14 d, and a higher color value of lightness (CIE $L^*$) compared to 100% $N_2$-MAP. The development of lipid oxidation measured by thiobarbituric acid reactive substance (TBARS) values seemed to be effectively controlled throughout the storage period in both 50% $CO_2$-MAP and 100% $N_2$-MAP regardless of the application of organic acids. The 50% $CO_2$-MAP inhibited the growth of aerobic and anaerobic bacteria (p<0.05) both in non-added and bacon added with organic acids mix. The 50% $CO_2$-MAP alone seemed to be effective in delaying the growth of bacteria since the use of organic acids mix gave no additional effects. The addition of organic acids mix lowered the pH value (p<0.05), effectively retarded the protein deterioration (p<0.05), and showed a higher color value of lightness (CIE $L^*$) value (p<0.05) and lower color value of redness (CIE $a^*$) value (p<0.05). In conclusion, 50% $CO^2$-MAP showed better quality and self-life of sliced bacon during storage. However, the beneficial effect of organic acids mix was not noticed in the concentration used in this experiment.

• The Journal of Korean Obstetrics and Gynecology
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• v.19 no.4
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• pp.47-60
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• 2006

Purpose : This study was conducted to investigate the inhibitory effects of Scutellaria barbata D. D on on the cell proliferation of HeLa Cells. Methods : Human uterine cervical carcinoma HeLa cells were cultured in the 1%, 5% and 10% concentration of Scutellaria barbata D. D on solution for 24, 48 and 72 hours for the direct inhibitory effects of Scutellaria barbata D. D on. Then we examined the effect of Scutellaria barbata D. D on solution on the cell proliferation inhibition by XTT assay. DNA fragmentation, MAP kinase activity and caspase activity by FACS analysis in HeLa cells. Results : We found that the proliferation of HeLa cells was significantly decreased in Scutellaria barbata D. D on solution containing groups comparing with a control group in a concentration-dependant manner. When HeLa cells were cultivated for 24 hours with 5% Scutellaria barbata D. D on solution containing group, the percentage of HeLa cells with activated caspase was the highest. Scutellaria barbata D. D on solution reduced the MAP kinase activity of HeLa cells comparing with the control group. By the XTT assay, the cell's activity was decreased in 5% and 10% Scutellaria barbata D. D on solution containing groups in 24 and 72 hours cultivation and 10% group in 48 hours. DNA fragmentation and caspase-3 activity of HeLa cells, however, were changed insignificantly. Conclusion : From this study we could suggest that Scutellaria barbata D. D on is available to the inhibition and apoptosis of human cervical carcinoma cell line, HeLa cells in vitro.