• Journal of Magnetics
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• 제21권1호
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• pp.125-132
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• 2016

High gradient magnetic separation (HGMS) is the most commonly used magnetic cell separation technique in biomedical science. However, parameters determining target cell capture efficiencies in HGMS are still not well understood. This limitation leads to loss of information and resources. The present study develops a bead-capture theory to predict capture efficiencies in HGMS. The theory is tested with CD3- and CD14-positive cells in combination with paramagnetic beads of different sizes and a generic immunomagnetic separation system. Data depict a linear relationship between normalized capture efficiency and the bead concentration. In addition, it is shown that key biological functions of target cells are not affected for all bead sizes and concentrations used. In summary, linear bead-capture theory predicts capture efficiency ($E_t$) in a highly significant manner.

• 설비공학논문집
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• 제22권11호
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• pp.773-780
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• 2010

The dispersion of plume which is emitted from a chimney is governed by a lot of factors： wind, local terrain, turbulence intensity of atmosphere, and temperature, etc. In this study, we numerically investigate the plume dispersions for various altitudinal temperature gradients and wind speeds. The normal atmosphere has the temperature decrease of $0.6^{\circ}C/100m$, however, actually the real atmosphere has the various altitudinal temperature profiles according to the meteorological factors. A previous study focused on this atmospheric temperature gradient which induces a large scale vertical flow motion in the atmosphere thus makes a peculiar plume dispersion characteristics. In this paper, the effects of the atmospheric temperature gradient as well as the wind speed are investigated concurrently. The results for the developing processes in the atmosphere and the affluent's concentrations at the ambient and ground level are compared under the various altitudinal temperature gradients and wind speeds.

• 환경영향평가
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• 제12권4호
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• pp.303-313
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• 2003

This study was carried out to survey the high ozone episode of summertime in Busan. The selected day was July 18, 1999 and August 24, 2001 which recorded exceed to 12ppb/hr at 3 station in Busan simultaneously. In case July 18, 1999, thick cloud and variable wind made weak ozone concentration during morning hour. And increase of ozone concentration by revolution of mixed layer for morning hour did not occur in this case study day. Photochemical reaction by strong radiation after 1100LST made sharp increase rate of ozone concentration(50ppb/hr). In case August 24, 2001, the meteorological condition of this day was not general wind with gradient force, very clear day with less cloud amount, high insolation and sunshine. Dongsamdong, Beomcheondong, Daeyeondong, and Sinpyeongdong had double peak which twice maximum concentration in the early afternoon and late afternoon. Ozone concentration of this day was in inverse proportion to Nitrogen oxide strongly. Ozone concentration exceed to 60ppb/hr occurred at 1400LST, continued to 2300LST.

• Structural Engineering and Mechanics
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• 제65권6호
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• pp.645-656
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• 2018

Importance of procuring adequate knowledge about the mechanical behavior of double-layered graphene sheets (DLGSs) incensed the authors to investigate wave propagation responses of mentioned element while rested on a visco-Pasternak medium under hygro-thermal loading. A nonlocal strain gradient theory (NSGT) is exploited to present a more reliable size-dependent mechanical analysis by capturing both softening and hardening effects of small scale. Furthermore, in the framework of a classical plate theory the kinematic relations are developed. Incorporating kinematic relations with the definition of Hamilton's principle, the Euler-Lagrange equations of each of the layers are derived separately. Afterwards, combining Euler-Lagrange equations with those of the NSGT the nonlocal governing equations are written in terms of displacement fields. Interaction of the each of the graphene sheets with another one is regarded by the means of vdW model. Then, a widespread analytical solution is employed to solve the derived equations and obtain wave frequency values. Subsequently, influence of each participant variable containing nonlocal parameter, length scale parameter, foundation parameters, temperature gradient and moisture concentration is studied by plotting various figures.

• 한국지하수토양환경학회:학술대회논문집
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• 한국지하수토양환경학회 2004년도 임시총회 및 추계학술발표회
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• pp.312-316
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• 2004

Single-well tracer tests, especially push pull tracer tests, are more effective to estimate hydraulic parameters and microbial metabolic activities in terms of duration and cost compared to multi-well tracer tests. However, there are some drawbacks in accuracy, complicated data analysis and uniqueness. These shortages are thought to be derived from the applied conditions which affect mass recovery curve and breakthrough curve. Factors such as extraction rate, resting period, hydraulic conductivity and hydraulic gradient are considered as the major factors determining the mass recovery rate and shape of the breakthrough curve. The results of the sensitivity analysis are summarized as follows: 1) the significant change in concentration of breakthrough curve is obtained when the extraction rate increases. This effect would also be much higher if the hydraulic conductivity is lower; 2) the mass recovery rate decreases with the increase of resting time, and the difference of mass recovery rates for different resting times is inversely proportional to the hydraulic conductivity; 3) the sensitivity values decrease with time. The hydraulic conductivity affects not only the early period, but the later period of the breakthrough curves; 4) The influence of the hydraulic gradient on the breakthrough curves is greater at earlier stage than at later stage. The mass recovery rate is inversely proportional to the hydraulic gradient.

• 한국물환경학회지
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• 제29권5호
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• pp.691-702
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• 2013

This review paper summarizes the theory, application, and potential drawbacks of diffusive gradient in thin film (DGT) probe which is a widely used in-situ passive sampling technique for monitoring inorganic contaminants in aquatic environments. The DGT probe employs a series of layers including a filter membrane, a diffusive hydrogel, and an ionic exchange resin gel in a plastic unit. The filter side is exposed to an aquatic environment after which dissolved inorganic contaminants, such as heavy metals and nuclides, diffuse through the hydrogel and are accumulated in the resin gel. After retrieval, the contaminants in the resin gel are extracted by strong acid or base and the concentrations are determined by analytical instruments. Then aqueous concentrations of the inorganic contaminants can be estimated from a mathematical equation. The DGT has also been used to monitor nutrients, such as ${PO_4}^{3-}$, in lakes, streams, and estuaries, which might be helpful in assessing eutrophic potential in aquatic environments. DGT is a robust in-situ passive sampling techniques for investigating bioavailability, toxicity, and speciation of inorganic contaminants in aquatic environments, and can be an effective monitoring tool for risk assessment.

• 한국농림기상학회지
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• 제9권4호
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• pp.268-276
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• 2007

본 연구에서는 서울 도심과 전원 지역간 $CO_2$ 농도 차이가 IPCC 중단기 기후변화 시나리오에 상응하는 차이가 있는지를 확인하기 위해 수도권의 도시기후를 IPCC 기후변화 시나리오를 재현한 "천연실험실"로 활용하고자 서울(도심), 수원(부도심), 이천(전원), 3지역에서 2006년 6월 19일부터 2007년 6월 25일(2007년 3월 3일-5월 22일까지 결측)까지 $CO_2$ 농도를 관측하였으며 이 결과를 요약하면 다음과 같다. 1) 3지점의 연 평균 대기 중 $CO_2$ 농도의 구배가 서울(439 ppm)>수원(419 ppm)>이천(416 ppm)으로 나타났다. 2) 계절에 따른 $CO_2$ 농도의 일변화 변동폭은 여름철에 크게 나타난 반면 겨울철에는 그 폭이 완만해졌다. 3) 계절에 따른 시간대별 대기 중 $CO_2$ 농도는 여름철에는 대체로 새벽 5시에, 가을철에는 오전 8시에, 겨울철에는 오전 9시에, 봄철에는 새벽 5시에 고농도가 나타남으로써 고농도가 나타나는 시간대가 가을철부터 지연되었다가 다시 봄철에는 일출 전후로 옮겨갔다. 4) 시간대별 대기 중 $CO_2$ 농도는 일출 직후에 442 ppm(서울: 0700 LST, Local Standard Time)으로 최대 농도를 보였으며, 최소 $CO_2$ 농도는 광합성이 활발한 오후 시간대로 407 ppm(수원: 1500 LST)으로 나타났다. 5) 배경대기관측소 안면도에서 산정한 배경 $CO_2$ 연평균 농도 377 ppm에 대해 서울은 14%, 수원은 10%, 이천은 9% 높았으며, IPCC BAU Scenario의 2030-2040년 대기 중 $CO_2$ 농도인 450 ppm에 대해 서울은 이미 98%에 도달하였고, 2040-2050년의 550 ppm에 대해서는 80%에 도달하여 우리나라의 도시-전원(서울-이천)간 $CO_2$ 농도차이는 IPCC BAU Scenario의 20-30년 후의 기후변화 시나리오에 상응하는 것으로 판단된다.

• 한국동물생명공학회지
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• 제35권1호
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• pp.58-64
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• 2020

This present study was conducted to investigate protective effect of discontinuous Percoll gradient containing alpha-linolenic acid (ALA) before freezing process on viability, acrosome damage, mitochondrial activity, and oxidative stress of frozen-thawed boar spermatozoa. The separation of spermatozoa by discontinuous Percoll gradient was performed by different concentration of Percoll solution (45/90%) containing ALA combined with bovine serum albumin (BSA), and collected sperm in each Percoll layer was cryopreserved. To evaluate viability, acrosome damage, mitochondrial activity, and reactive oxygen species (ROS) level of frozen-thawed sperm, flow cytometry was used. Morphological abnormalities were observed under light microscope. In results, viability of sperm from 90% Percoll layer was higher than control and 45% Percoll group (p < 0.05). Separated sperm in 90% Percoll layer had lower acrosome damage and morphological abnormalities than control as well as viability, whereas 45% Percoll group was higher (p < 0.05). Similar with acrosome damage and abnormalities, mitochondrial activity was slightly enhanced and the population of live sperm with high ROS level was decreased by 90% Percoll separation, however, there was no significant difference. Supplementation of 3 ng/mL ALA into Percoll solution increased sperm viability and decreased population of live sperm with high ROS compared to control (p < 0.05). In conclusion, discontinuous Percoll gradient before freezing process could improve efficiency of cryopreservation of boar sperm through selection of sperm with high freezing resistance, and supplement of ALA during Percoll gradient might contribute suppression of ROS generation via stabilizing of plasma membrane during cryopreservation.

• KSBB Journal
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• 제15권3호
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• pp.219-225
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• 2000

Bacillus thuringiensis를 생물농약으로 이용하기 위해서는 고농도의 포자형성에 의한 높은 살충성의 8 -endotoxin를 생산하는 것이 중요하다. 따라서 본 연구에서는 B. thuringiensis의 고농도 배양에 의한 높은 포자형성 수율을 얻기 위하여 40%의 용존산 소량과 $28^{\circ}C$에서 여랴 가지 방법의 유가식 배양법을 검토하였다. 최종 배양액의 glucose 농도가 50 g/L가 되도록 하는 경우의 유가식 배양에서는 continuos fed-batch culture의 linear gradient f feeding 법에 의하여 $9.37{\times}109$ cell/mL의 최대 생존 세포 수와 8 8.33 X 109 spore/mL의 최대 포자 수를 얻었으며, 이때의 포자 형 성율은 88.9% 이었다 최종 배양액의 glucose 농도가 100 m/L가 되도록 하는 경우의 유가식 배양에서는 intermittent fed-batch culture의 pH-stat 법에 의하여 $1.35{\times}1010$cell/mL의 최대 생존 세포 수와 $1.35{\times}1010$ spore/mL의 최대 포자 수를얻었으며, 이 때의 포자 형성율은 97.8% 이엇다.

• 한국막학회:학술대회논문집
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• 한국막학회 1996년도 춘계 총회 및 학술발표회
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• pp.5-10
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• 1996

Active transport is one of the essential function of biological membrane transport systems, in which substrates are transported from lower to higher concentration side against their concentration gradient. In this article, we describe photo- and pH-induced active transport models by designing the functional carriers based on the affinity-switching strategy.