• Title/Summary/Keyword: Computational Science application

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Validation and Application of a Real-time PCR Protocol for the Specific Detection and Quantification of Clavibacter michiganensis subsp. sepedonicus in Potato

  • Cho, Min Seok;Park, Duck Hwan;Namgung, Min;Ahn, Tae-Young;Park, Dong Suk
    • The Plant Pathology Journal
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    • v.31 no.2
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    • pp.123-131
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    • 2015
  • Clavibacter michiganensis subsp. sepedonicus (Cms) multiplies very rapidly, passing through the vascular strands and into the stems and petioles of a diseased potato. Therefore, the rapid and specific detection of this pathogen is highly important for the effective control of the pathogen. Although several PCR assays have been developed for detection, they cannot afford specific detection of Cms. Therefore, in this study, a computational genome analysis was performed to compare the sequenced genomes of the C. michiganensis subspecies and to identify an appropriate gene for the development of a subspecies-specific PCR primer set (Cms89F/R). The specificity of the primer set based on the putative phage-related protein was evaluated using genomic DNA from seven isolates of Cms and 27 other reference strains. The Cms89F/R primer set was more specific and sensitive than the existing assays in detecting Cms in in vitro using Cms cells and its genomic DNA. This assay was also able to detect at least $1.47{\times}10^2copies/{\mu}l$ of cloned-amplified target DNA, 5 fg of DNA using genomic DNA or $10^{-6}$ dilution point of 0.12 at $OD_{600}$ units of cells per reaction using a calibrated cell suspension.

High-precision modeling of uplift capacity of suction caissons using a hybrid computational method

  • Alavi, Amir Hossein;Gandomi, Amir Hossein;Mousavi, Mehdi;Mollahasani, Ali
    • Geomechanics and Engineering
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    • v.2 no.4
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    • pp.253-280
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    • 2010
  • A new prediction model is derived for the uplift capacity of suction caissons using a hybrid method coupling genetic programming (GP) and simulated annealing (SA), called GP/SA. The predictor variables included in the analysis are the aspect ratio of caisson, shear strength of clayey soil, load point of application, load inclination angle, soil permeability, and loading rate. The proposed model is developed based on well established and widely dispersed experimental results gathered from the literature. To verify the applicability of the proposed model, it is employed to estimate the uplift capacity of parts of the test results that are not included in the modeling process. Traditional GP and multiple regression analyses are performed to benchmark the derived model. The external validation of the GP/SA and GP models was further verified using several statistical criteria recommended by researchers. Contributions of the parameters affecting the uplift capacity are evaluated through a sensitivity analysis. A subsequent parametric analysis is carried out and the obtained trends are confirmed with some previous studies. Based on the results, the GP/SA-based solution is effectively capable of estimating the horizontal, vertical and inclined uplift capacity of suction caissons. Furthermore, the GP/SA model provides a better prediction performance than the GP, regression and different models found in the literature. The proposed simplified formulation can reliably be employed for the pre-design of suction caissons. It may be also used as a quick check on solutions developed by more time consuming and in-depth deterministic analyses.

Analysis of the Scattering Property of Dielectric Scatterer with Impedance Boundary Condition (임피던스 경계면 조건을 적용한 유전체의 산란 특성 분석)

  • Hwang, Ji-Hwan;Park, Sin-Myeong;Oh, Yisok
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.25 no.10
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    • pp.1087-1094
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    • 2014
  • An numerical technique of impedance boundary condition to improve an efficiency in the process of moment method with CFIE(Combined Field Integral Equation), which is widely used to analyze the scattering property of dielectric scatterers, and results of its cross-validations are presented in this study. Application of the impedance boundary allows to represent the equivalent surface currents of dielectric scatterer depicted by both kinds of electric/magnetic surface currents(Js, Ms) to the single surface current by Js or Ms only. Accuracy of this technique is validated by the existing CFIE and theoretical values such as Mie-series solution and small perturbation scattering model. The computational difference of less than 1 dB was verified within an imaginary part of dielectric constant more than 12, as well.

A Simulation Study on Transcranial Direct Current Stimulation Using MRI in Alzheimer's Disease Patients (알츠하이머병 환자의 MRI를 활용한 경두개 직류 전기 자극 시뮬레이션에 관한 연구)

  • Chae-Bin Song;Cheolki Lim;Jongseung Lee;Donghyeon Kim;Hyeon Seo
    • Journal of Biomedical Engineering Research
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    • v.44 no.6
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    • pp.377-383
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    • 2023
  • Purpose: There is increasing attention to the application of transcranial direct current stimulation (tDCS) for enhancing cognitive functions in subjects to aging, mild cognitive impairment (MCI), and Alzheimer's disease (AD). Despite varying treatment outcomes in tDCS which depend on the amount of current reaching the brain, there is no general information on the impacts of anatomical features associated with AD on tDCS-induced electric field. Objective: The objective of this study is to examine how AD-related anatomical variation affects the tDCS-induced electric field using computational modeling. Methods: We collected 180 magnetic resonance images (MRI) of AD patients and healthy controls from a publicly available database (Alzheimer's Disease Neuroimaging Initiative; ADNI), and MRIs were divided into female-AD, male-AD, female-normal, and male-normal groups. For each group, segmented brain volumes (cerebrospinal fluid, gray matter, ventricle, rostral middle frontal (RMF), and hippocampus/amygdala complex) using MRI were measured, and tDCS-induced electric fields were simulated, targeting RMF. Results: For segmented brain volumes, significant sex differences were observed in the gray matter and RMF, and considerable disease differences were found in cerebrospinal fluid, ventricle, and hippocampus/amygdala complex. There were no differences in the tDCS-induced electric field among AD and normal groups; however, higher peak values of electric field were observed in the female group than the male group. Conclusions: Our findings demonstrated the presence of sex and disease differences in segmented brain volumes; however, this pattern differed in tDCS-induced electric field, resulting in significant sex differences only. Further studies, we will adjust the brain stimulation conditions to target the deep brain and examine the effects, because of significant differences in the ventricles and deep brain regions between AD and normal groups.

State of Information Technology and Its Application in Agricultural Meteorology (농업기상활용 정보기술 현황)

  • Byong-Lyol Lee;Dong-Il Lee
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.6 no.2
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    • pp.118-126
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    • 2004
  • Grid is a new Information Technology (IT) concept of "super Internet" for high-performance computing: worldwide collections of high-end resources such as supercomputers, storage, advanced instruments and immerse environments. The Grid is expected to bring together geographically and organizationally dispersed computational resources, such as CPUs, storage systems, communication systems, real-time data sources and instruments, and human collaborators. The term "the Grid" was coined in the mid1990s to denote a proposed distributed computing infrastructure for advanced science and engineering. The term computational Grids refers to infrastructures aimed at allowing users to access and/or aggregate potentially large numbers of powerful and sophisticated resources. More formally, Grids are defined as infrastructure allowing flexible, secure, and coordinated resource sharing among dynamic collections of individuals, institutions and resources referred to as virtual Organizations. GRID is an emerging IT as a kind of next generation Internet technology which will fit very well with agrometeorological services in the future. I believe that it would contribute to the resource sharing in agrometeorology by providing super computing power, virtual storage, and efficient data exchanges, especially for developing countries that are suffering from the lack of resources for their agmet services at national level. Thus, the establishment of CAgM-GRID based on existing RADMINSII is proposed as a part of FWIS of WMO.part of FWIS of WMO.

Analysis on the Hydroelasticity of Whole Ship Structure by Coupling Three-dimensional BEM and FEM (3차원 경계요소법과 전선 유한요소 해석의 연성을 통한 전선 유탄성 해석)

  • Kim, Kyong-Hwan;Bang, Je-Sung;Kim, Yong-Hwan;Kim, Seung-Jo
    • Journal of the Society of Naval Architects of Korea
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    • v.49 no.4
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    • pp.312-326
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    • 2012
  • This paper considers a fully coupled 3D BEM-FEM analysis for the ship structural hydroelasticity problem in waves. Fluid flows and structural responses are analyzed by using a 3D Rankine panel method and a 3D finite element method, respectively. The two methods are fully coupled in the time domain using a fixed-point iteration scheme, and a relaxation scheme is applied for improve convergence. In order to validate the developed method, numerical tests are carried out for a barge model. The computed natural frequency, motion responses, and time histories of stress are compared with the results of the beam-based hydroelasticity program, WISH-FLEX, which was thoroughly validated in previous studies. This study extends to a real-ship application, particularly the springing analysis for a 6500 TEU containership. Based on this study, it is found that the present method provides reliable solutions to the ship hydroelasticity problems.

Analysis of Performance of Patent for National R&D Project of ICT (ICT 분야 국가 R&D 과제의 특허 성과 분석)

  • Kim, Byeong-Jeong;Shon, Young-Woo;Bae, Young-Chul
    • The Journal of the Korea institute of electronic communication sciences
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    • v.9 no.10
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    • pp.1161-1168
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    • 2014
  • As rapidly growing industry, ICT industry is emphasized for its importance and the demand to figure out for the performances of investment of R&D for ICT have been increasing. The performance for investment of R&D analyze mainly as commercialization, sales, hiring employee and intellectual property and so on. In this paper, we propose an analytical method for performance as focusing an application time of patent that are acquired as a result after perform the national project of ICT. We classify 4 subdivision technologies and 17 detailed classification of Korean industrial standard for 35,551 item of ICT area among national R&D project. This paper proposes computational method for required time about patent's performance. As a analyzed result we verify that the activity of technology's commercialization is most active in communication as 1.2 year of ICT among national project.

Computational Fluid Dynamic Modeling for Internal Antenna Type Inductively Coupled Plasma Systems (CFD를 이용한 내장형 안테나 유도 결합 플라즈마 시스템 모델링)

  • Joo, Jung-Hoon
    • Journal of the Korean Vacuum Society
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    • v.18 no.3
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    • pp.164-175
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    • 2009
  • CFD is used to analyze gas flow characteristics, power absorption, electron temperature, electron density and chemical species profile of an internal antenna type inductively coupled plasma system. An optimized grid generation technology is used for a complex real-scale models for industry. A bare metal antenna shows concentrated power absorption around rf a feeding line. Skin depth of power absorption for a system is modeled to 50 mm, which is reported 53 mm by experiments. For an application of bipolar plates for hydrogen fuel cells, multi-sheet loading ICP nitriding system is proposed using an internal ICP antenna. It shows higher atomic nitrogen density than reported simple pulsed dc nitriding systems. Minimum gap between sheets for uniform nitriding is modeled to be 39 mm.

Research on Improvement of Efficiency in Flat Plate Solar Collector by Using Double-Wall Glazing and VIP Insulation (이중투과체 및 VIP복합 단열재 적용 평판 집열기의 성능 향상에 대한 연구)

  • Lee, Doo Ho;Jang, Han Bin;Kim, Young Hak;Do, Kyu Hyung;Lee, Kwang Seob;Lyu, Nam Jin
    • Korean Journal of Air-Conditioning and Refrigeration Engineering
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    • v.28 no.11
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    • pp.458-465
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    • 2016
  • The purpose of this research is to improve the thermal effiency of solar collector and to quantitatively analyze its performance. Solar thermal systems have been limited to water heating systems mainly using low-temperature range. However, through diverse developments, the application has been extended to medium- and high-temperature fields such as solar heating, solar air conditioning, and solar thermal industrial process. Among the diverse research, this research is specially focusing on enhancement of the thermal performance by minimizing the heat loss coefficient of flat plate solar collectors. In order to do it, a front-side glazing material and a back-side insulation material with high insulated structure is proposed and based on computational analysis, the performance of energy collecting volume of the proposed solar collector is analyzed. The research shows that the proposed structure has the excellent performance at medium- and high-temperature range. therefore, it is expected that the proposed structure can easily replace existing technologies.

The Bi-directional Least Mean Square Algorithm and Its Application to Echo Cancellation (양방향 최소 평균 제곱 알고리듬과 반향 제거로의 응용)

  • Kwon, Oh-Sang
    • The Journal of the Korea institute of electronic communication sciences
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    • v.9 no.12
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    • pp.1337-1344
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    • 2014
  • The objective of an echo canceller connected to any end of a communication line such as digital subscriber line (DSL) is to compensate the outgoing transmit signal in the receiving path that the hybrid circuit leaks. The echo canceller working in a full duplex environment is an adaptive system driven by the local signal. Conventional echo canceller that implement the least mean square (LMS) algorithm provides a low computational burden but poor convergence properties. The length of the echo canceller will directly affect both the degree of performance and the convergence speed of the adaptation process. To cancel long time-varying echoes, the number of tap coefficients of a conventional echo canceller must be large, which decreases the convergence speed of the adaptive filter. This paper proposes an alternative technique for the echo cancellation in a telecommunication channel. The new technique employs the bi-directional least mean square (LMS) algorithm for adaptively computing the optimal set of the coefficients of the echo canceller, which is composed of weighted combination of both feedforward and feedback algorithms. Finally, Simulation results as well as mathematical analysis demonstrates that the proposed echo canceller has faster convergence speed than the conventional LMS echo canceller with nearly equivalent complexity of computation.