• Bulletin of the Korean Chemical Society
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• v.22 no.1
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• pp.46-52
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• 2001

To evaluate the non-ionic polymer, poly(ethylene glycol) (PEG), as a component in cationic copolymers for non-viral gene delivery systems, PEG was coupled to polyethylenimine (PEI). We present the effects of different degrees and shapes of pegylation of PEI on cytotoxicity, water solubility and transfection efficiency. This work reports the synthesis and characterization of a series of cationic copolymers on the basis of the conjugates of PEI with PEG. The modified molecules were significantly less toxic than the original polymer. Moreover, the chemical modification led to enhancement of their solubility. The comparison of pegylated PEIs with different degrees of derivation showed that all the polymers tested reached comparable levels of transgene expression to that of native PEI. As assessed by agarose gel electrophoresis, even highly substituted PEI derivatives were still able to form polyionic complexes with DNA. However, aside from an increase in solubility and retention of the ability to condense DNA, methoxy-PEG-modified PEIs resulted in a significant decrease in the transfection activity of the DNA complexes. In fact, the efficiency of the copolymer was compromised even at a low degree of modification suggesting that the PEG action resulting from its shape is important for efficient gene transfer. The mode of PEG grafting and the degree of modification influenced the transfection efficiency of PEI.

• Journal of the Korean Society of Manufacturing Process Engineers
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• v.18 no.8
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• pp.8-17
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• 2019

In this study, a model reduction technique was used to develop a precise noise and vibration prediction model for the individual components of a driveline system. The dynamic reduced-order model generated by the Craig-Bampton method was applied to perform dynamic analysis of an electric agricultural power cart. The natural frequency and acceleration response results were analyzed according to the different number of dominant sub-structural modes contained in the reduced-order models. Through the analysis results, it was confirmed that a sufficient number of dominant sub-structures to satisfy the operating conditions should be selected to construct an optimal reduced-order model.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.30 no.4
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• pp.106-113
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• 2002

In spacecraft system, structure subsystem has the mission of supporting all the components safely under various space environmental conditions. The safety of spacecraft structure is finally verified from the coupled load analysis, which is a branch of load analysis which combines the launch vehicle and satellite. This study introduces the optimization algorithm to reduce the weight of spacecraft structure under launch environmental conditions directly. The acceleration responses are obtained by the introduction of coupled load analysis, which lead to check the failure of spacecraft structural members. The results show a 12% saving of structural weight and this saving is mainly driven by the thickness of honeycomb core, which strongly affects the natural frequencies of platforms and panels.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.35 no.3
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• pp.175-182
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• 2022

In many engineering problems, the dynamic substructuring can be useful to analyze complex structures which made with many substructures, such as aircrafts and automotive vehicles. It was originally intended as a method to simplify the engineering problem. The powerful advantage to this is that computational efficiency dramatically increases with eliminating unnecessary degrees-of-freedom of the system and the system targets are concurrently satisfied. Craig-Bampton method has been widely used for the linear system reduction. Recently, multi-level optimization (such as target cascading), which propagates the system-level targets to the subsystem-level targets, has been widely utilized. To this concept, the genetic algorithm which one of the global optimization technique has been utilized to the substructure optimization. The number of internal modes for each substructure can be obtained by the genetic algorithm. Simultaneously, the reduced system meets the top-level targets. In this paper, various numerical examples are tested to verify this concept.

• Tribology and Lubricants
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• v.36 no.2
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• pp.105-115
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• 2020

This paper presents a numerical study on the rotordynamic analysis of a dual-spool turbofan engine in the context of blade defect events. The blades of an axial-type aeroengine are typically well aligned during the compressor and turbine stages. However, they are sometimes exposed to damage, partially or entirely, for several operational reasons, such as cracks due to foreign objects, burns from the combustion gas, and corrosion due to oxygen in the air. Herein, we designed a dual-spool rotor using the commercial 3D modeling software CATIA to simulate blade defects in the turbofan engine. We utilized the rotordynamic parameters to create two finite element Euler-Bernoulli beam models connected by means of an inter-rotor bearing. We then applied the unbalanced forces induced by the mass eccentricities of the blades to the following selected scenarios: 1) fully balanced, 2) crack in the low-pressure compressor (LPC) and high pressure compressor (HPC), 3) burn on the high-pressure turbine (HPT) and low pressure compressor, 4) corrosion of the LPC, and 5) corrosion of the HPC. Additionally, we obtained the transient and steady-state responses of the overall rotor nodes using the Runge-Kutta numerical integration method, and employed model reduction techniques such as component mode synthesis to enhance the computational efficiency of the process. The simulation results indicate that the high-vibration status of the rotor commences beyond 10,000 rpm, which is identified as the first critical speed of the lower speed rotor. Moreover, we monitored the unbalanced stages near the inter-rotor bearing, which prominently influences the overall rotordynamic status, and the corrosion of the HPC to prevent further instability. The high-speed range operation (>13,000 rpm) coupled with HPC/HPT blade defects possibly presents a rotor-case contact problem that can lead to catastrophic failure.