• Journal of Plant Biotechnology
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• v.36 no.2
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• pp.144-148
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• 2009

The transfer of Mn-Superoxide Dismutase (SOD2) gene, complex gene (SA) of CuZnSOD and ascorbate peroxidase (APX), and NDP kinase 2 (NDPK2) gene into Korean 4 cultivars (cvs. Millenium White, Glory Blue, Glory Red, and Glory Purple) and 15 purebred lines of petunia was conducted using Agrobaterium-mediated technique. Two (Wongyo A2-16 and A2-36) of 15 purebred lines and one (cv. Glory Red) of 4 cultivars were effective for the transfer of SOD2 gene. The putative transgenic plants survived on the 2nd selection medium were 124. From PCR analysis, 118 (derived from 4 cultivars and 2 purebred lines) of 124 plants were confirmed to contain marker (npt II ) gene, while 58 of 118 plants did not have target genes. There were no plants with both npt II and SA genes. Twenty seven of 28 SOD2 transgenic plants were re-confirmed as transformants by Sothern analysis. SOD2 and NDPK2 genes were expressed in the transgenic petunias as the ratio of 77.8 to 100.0 % and 23.5%, respectively. T1 seeds were obtained from 36 acclimated transgenic plants (SOD2 34 plus NDPK2) in a glasshouse by self-pollination.

• Journal of Advanced Marine Engineering and Technology
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• v.11 no.1
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• pp.72-79
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• 1987

When the wall temperature is very high, a stable vapor film covers the heat transfer surface. The vapor film creates a strong thermal resistance when heat is transferred to the liquid though it. This phenomenon, called "film boiling" is very important in the heat treatment of metals, the design of cryogenic heat exchangers, and the emergency cooling of nuclear reactors. In the practical engineering problems of the transient cooling process of a high temperature wall, the wall temperature history, the variation of the heat transfer coefficients, and the wall superheat at the rewetting points, are the main areas of concern. These three areas are influenced in a complex fashion such factors as the initial wall temperature, the physical properties of both the wall and the coolant, the fluid temperature, and the flow state. Therefore many kinds of specialized experiments are necessary in the creation of precise thermal design. The object of this study is to investigate the heat transfer characteristics in the transient cooling process of a high temperature wall. The slow transient cooling experiment was carried out with a copper block of high thermal capacity. The block was 240 mm high and 79 mm O.D.. The coolant flowed throuogh the center of a 10 mm diameter channel in the copper block. In the copper block, three sheathed thermocouples were placed in a line perpendicular to the flow. These thermocouples were used to take measurements of the temperature histories of the copper block.

• Bulletin of the Korean Chemical Society
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• v.25 no.10
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• pp.1503-1507
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• 2004

New synthetic methodology of the saturated and unsaturated Er(III)-chelated prototype complexes based on benzoate and pentafluorobenzoate ligands was developed through ligand-exchange reaction. The saturated 8-coordinated Er(III) complexes exhibit stronger near-IR emission than those of the unsaturated 6-coordinated Er(III) complexes, obtained from the direct photoexcitation of Er ions with 488 nm. Three $H_2O$ molecules coordinated in the unsaturated 6-coordinated complexes seriously quenched the near IR emission by the harmonic vibration relaxation decay of O-H bonds. Also, the stronger emission of the Er(III) complexes was obtained by the indirect photoexcitation of ligands than by the direct photoexcitation of the Er(III) ions, due to the energy transfer between the excited ligand and the erbium ion. Furthermore, the saturated Er(III)-chelated complex with C-F bonds shows much stronger near IR emission than that of the saturated Er(III)-chelated complex with C-H bonds. It is attributed to the influence of C-F bonds on near IR emission.

• Journal of the Korean Society of Propulsion Engineers
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• v.23 no.2
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• pp.27-37
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• 2019

The shell and tube heat exchangers installed in the propulsion system test complex (PSTC) at the Naro Space Center heats cryogenic helium to 500 K with a heat transfer oil. As the experimental helium outlet temperature was lower than expected (less than 100 K), the boundary layer effect of the heat transfer oil is predicted to be the cause of the performance deterioration. A computational fluid dynamics (CFD) analysis was performed to verify where the boundary layer effect exists; however, the boundary layer effect has no significant impact on the performance of the heat exchanger. An alternative method to improve the performance of the heat exchanger by changing the heat transfer oil has been discussed in this paper. The low viscosity and high thermal conductivity at high temperature (~500 K) of heat transfer oil at the shell-side are required to improve the thermal performance of the heat exchanger. The experimental performance of the heat exchanger, used to exchange heat between the cryogenic helium and hot heat transfer oil at the PSTC are summarized in this paper.

• Asian-Australasian Journal of Animal Sciences
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• v.16 no.10
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• pp.1411-1420
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• 2003

Comparative maps, representing chromosomal locations of homologous genes in different species, are useful sources of information for identifying candidate disease genes and genes determining complex traits. They facilitate gene mapping and linkage prediction in other species, and provide information on genome organization and evolution. Here, the current gene mapping and comparative mapping status of the major livestock species are presented. Two techniques were widely used in comparative mapping: FISH (Fluorescence In Situ Hybridization) and PCR-based mapping using somatic cell hybrid (SCH) or radiation hybrid (RH) panels. New techniques, using, for example, ESTs (Expressed Sequence Tags) or CASTS (Comparatively Anchored Sequence Tagged Sites), also have been developed as useful tools for analyzing comparative genome organization in livestock species, further enabling accurate transfer of valuable information from one species to another.

• 한국정보디스플레이학회:학술대회논문집
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• 2009.10a
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• pp.346-349
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• 2009

We investigated the doping effects of $ReO_3$ in different p-type organic semiconductors on the formation of charge transfer complexes and the electrical conductivity by comparing the absorption in ultraviolet-visible-nearinfrared (UV-Vis-NIR) and the current density-voltage characteristics of the hole only devices, respectively. The large energy difference between the HOMO level of host and Fermi energy level of dopant (${\Delta}E$=$E_{HOHO,host}$ - $E_{F,dopant}$) gives higher concentration of CT complexes and enhanced conductivity.

• Applied Chemistry for Engineering
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• v.9 no.6
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• pp.811-816
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• 1998

Rebar corrosion in cement mortar containing chloride ions was investigated by electrochemical AC impedance spectroscopy. Corrosion of mild steel bar was accelerated by an acceleration test equipment in short period. Impedance values obtained from AC-impedance method could be adapted to the proposed electrochemical equivalent circuit model and they were consistent with calculated values obtained by CNLS fitting method. The weight loss of rebar could be calculated by charge transfer resistance($R_2$) with time and it was close to real value.

• Journal of Microbiology and Biotechnology
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• v.16 no.5
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• pp.795-798
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• 2006

The phosphoenolpyruvate-dependent carbohydrate phosphotransferase system (PTS) is responsible for the simultaneous transfer and phosphorylation of various carbon sources in Escherichia coli. The ptsG gene encoding the enzyme $IICB^{Glc}$, the membrane component of the glucose-specific PTS, is repressed by Mlc and activated by the CRP cAMP complex; various other factors, such as Fis, FruR, and ArcA, are also known to be involved in ptsG regulation. Thus, in an attempt to discover a novel gene affecting the regulation of ptsG, a mutant with a decreased ptsG transcription in the presence of glucose compared with the wild-type strain was screened using transposon random mutagenesis. The mutant was found to have a transposon insertion in yhjV, a putative gene encoding a transporter protein whose function is yet unknown.

• Bulletin of the Korean Chemical Society
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• v.31 no.4
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• pp.934-940
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• 2010

The reduction of [$Os_3(CO)_{11}P(OMe)_3$] and subsequent ionic coupling of the reduced species with $[Ru({\eta}^5-C_5H_5)(CH_3CN)_3]^+$ resulted in the formation of [$Os_3(CO)_{11}P(OMe)_3(Ru({\eta}^5-C_5H_5))_2$] which can be converted to spiked tetrahedral cluster, [$HOs_3(CO)_{11}P(OMe)_3Ru_2({\eta}^5-C_5H_5)(C_5H_4)$] via the intramolecular hydrogen transfer. Due to the unavailability of a suitable single crystal, the PW91/SDD and LDA/SDD density functional methods were used to predict possible structures and the available spectroscopic information (IR, NMR) of [$Os_3(CO)_{11}P(OMe)_3(Ru({\eta}^5-C_5H_5))_2$]. The most probable geometry found by constrained search is the isomer (a2) in which the phosphite, $P(OMe)_3$, occupies an axial position on one of the two osmium atoms that is edge bridged by the $Ru(CO)_2({\eta}^5-C_5H_5)$ unit. By using the most probably geometry, the predicted infrared frequencies and $^1H$, $^{13}C$ and $^{31}P$ NMR chemical shifts of the compound are in the same range as the experimental values. For this type of complex, the LDA/SDD method is appropriate for IR predictions whereas the OPBE/IGLO-II method is appropriate for NMR predictions. The activation energy and reaction energy of the intramolecular hydrogen transfer coupled with the structural change of the transition metal framework were estimated at the PW91/SDD level to be 110.32 and -0.14 kcal/mol respectively.

• BMB Reports
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• v.42 no.5
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• pp.245-259
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• 2009

The process by which adult neural stem cells generate new and functionally integrated neurons in the adult mammalian brain has been intensely studied, but much more remains to be discovered. It is known that neural progenitors progress through distinct stages to become mature neurons, and this progression is tightly controlled by cell-cell interactions and signals in the neurogenic niche. However, less is known about the cell-intrinsic signaling required for proper progression through stages of adult neurogenesis. Techniques have recently been developed to manipulate genes specifically in adult neural stem cells and progenitors in vivo, such as the use of inducible transgenic mice and viral-mediated gene transduction. A critical mass of publications utilizing these techniques has been reached, making it timely to review which molecules are now known to play a cell-intrinsic role in regulating adult neurogenesis in vivo. By drawing attention to these isolated molecules (e.g. Notch), we hope to stimulate a broad effort to understand the complex and compelling cascades of intrinsic signaling molecules important to adult neurogenesis. Understanding this process opens the possibility of understanding brain functions subserved by neurogenesis, such as memory, and also of harnessing neural stem cells for repair of the diseased and injured brain.