• Archives of Pharmacal Research
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• 제25권6호
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• pp.807-816
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• 2002

Dihydrofolate reductases (DHFR) of human, Candida albicans and E. coli were docked with their original ligands of X-ray crystal complex using QXP (Quick eXPlore), a docking program. Conditions to reproduce the crystal structures within the root mean square deviation (rmsd) of 2.00 $\AA$ were established. Applying these conditions, binding modes and species-specificities of a novel antibacterial compound, $N^4-(2-acetoxyethoxymethyl)-2-acetylpyridine$ thiosemicarbazone (MTSC), were studied. As the results, the docking program reproduced the crystal structures with average rmsd of six ligands as 0.91 $\AA$ ranging from 0.49 to 1.45 $\AA$. The interactions including the numbers of hydrogen bonds and hydrophobic interactions were the same as the crystal structures and superposition of the crystal and docked structures almost coincided with each other. For AATSC, the results demonstrated that it could bind to either the substrate or coenzyme sites of DHFR in all three species with different degrees of affinity. It confirms the experimentally determined kinetic behavior of uncompetitive inhibition against either the inhibitor or the coenzyme. The docked MTSC overlapped well with the original ligands and major interactions were consistent with the ones in the crystal complexes. The information generated from this work should be useful for future development of antibacterial and antifungal agents.

• International Journal of Industrial Entomology and Biomaterials
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• 제5권2호
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• pp.141-151
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• 2002

The success of sericulture industry in India is mainly attributed to the well-planned annual sericultural activity and the systematic implementation of pest preventive and control measures. The insect spectrum of silkworm and its food plants is complex and plays a major role in limiting the production of silk. Insects cause extensive damage to plant whereas predators and parasites either kill the silkworm larvae or force them to spin flimsy cocoons. Unilateral control measure against this pest is mainly based on the use of synthetic organic insecticides. Though these approaches initially paid rich dividends, the undesirable consequences soon surfaced. Insecticide induced resurgence of gall midges, leafhopper, leaf roller, secondary pest out breaks and development of pest biotypes has led to realization of Integrated Pest Management in sericulture. Various components of IPM, viz. Host plant resistance, cultural practices, biological control, chemical control and integrating them at various technological levels have been studied. Sources of host plant resistance have been identified for some of the major insect pests. High yielding mulberry variety has been propagated and their resistances towards major pests have been recorded. Cultural practices like pruning, pollarding, judicious use of nitrogen, optimum spacing and weed management have preyed to be the powerful tools in containing pests. Natural control over the pest population build- up exerted by the wide range of parasitoids, predators and pathogens has been well documented with identification of natural enemies and studies on their potential. Augmentation, through inoculation or inundative releases of parasitic arthropods, is the most direct way of increasing the numbers of these beneficials in sericulture.

• Clinical and Experimental Pediatrics
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• 제57권8호
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• pp.337-344
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• 2014

Inherited bone marrow failure syndrome (IBMFS) encompasses a heterogeneous and complex group of genetic disorders characterized by physical malformations, insufficient blood cell production, and increased risk of malignancies. They often have substantial phenotype overlap, and therefore, genotyping is often a critical means of establishing a diagnosis. Current advances in the field of IBMFSs have identified multiple genes associated with IBMFSs and their pathways: genes involved in ribosome biogenesis, such as those associated with Diamond-Blackfan anemia and Shwachman-Diamond syndrome; genes involved in telomere maintenance, such as dyskeratosis congenita genes; genes encoding neutrophil elastase or neutrophil adhesion and mobility associated with severe congenital neutropenia; and genes involved in DNA recombination repair, such as those associated with Fanconi anemia. Early and adequate genetic diagnosis is required for proper management and follow-up in clinical practice. Recent advances using new molecular technologies, including next generation sequencing (NGS), have helped identify new candidate genes associated with the development of bone marrow failure. Targeted NGS using panels of large numbers of genes is rapidly gaining potential for use as a cost-effective diagnostic tool for the identification of mutations in newly diagnosed patients. In this review, we have described recent insights into IBMFS and how they are advancing our understanding of the disease's pathophysiology; we have also discussed the possible implications they will have in clinical practice for Korean patients.

• 제어로봇시스템학회:학술대회논문집
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• 제어로봇시스템학회 2003년도 ICCAS
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• pp.333-336
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• 2003

For long time, the takeoff and landing control of airship was worked by human handling. With the development of the autonomous control system, the exact controls during the takeoff and landing were required and lots of methods and algorithms were suggested. This paper presents the result of airship take-off and landing by buoyancy control using air ballonet volume change and performance control of pitch angle for stable flight within the desired altitude. For the complexity of airship's dynamics, firstly, simple PID controller was applied. Due to the various atmospheric conditions, this controller didn’t give satisfactory results. Therefore, new control method was designed to reduce rapidly the error between designed trajectory and actual trajectory by learning algorithm using an artificial neural network. Generally, ANN has various weaknesses such as large training time, selection of neuron and hidden layer numbers required to deal with complex problem. To overcome these drawbacks, in this paper, the RBFN (radial basis function network) controller developed.

• 임상간호연구
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• 제19권1호
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• pp.33-44
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• 2013

Purpose: This study was done to develop a valid and reliable Classification System for Critical Care Nursing (CSCCN) to be used in Intensive Care Units (ICUs). Methods: Head nurses and staff nurses of 17 ICUs in 6 hospitals classified 307 patients to verify interrater reliability. To verify construct validity, the staff nurses classified 404 patients according to CSCCN comparing difference in medical department and type of stay in ICU. For conversion index, 78 patients from 4 ICUs of 'S' hospital were classified and nursing time was measured by 107 nurses and 18 nurse aids using stopwatches. Results: The developed CSCCN has 11 categories, 76 nursing activities and 101 criteria. The reliability was verified as having high agreement (r=.946). The construct validity was verified comparing differences in medical department and type of stay in ICU. According to scores, four groups in the CSCCN classification were identified. According to the conversion index, one score on the CSCCN means 7.2 minutes of nursing time. Conclusion: CSCCN can be used to measure diverse and complex nursing demands including psycho-social aspects of ICU patients and convert nursing demands to numbers.

• Mycobiology
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• 제29권2호
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• pp.80-84
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• 2001

Nine species of genus Ganoderma different in cultural characteristics each other were separated by histo-anatomical differences. Differences among the species and geographic distribution of G. lucidum were also analyzed to provide the criteria for the classification of Korean cultivation or wild type strains. Korean cultivation and wild type strains were quiet different from other species as well as Taiwan and North American G. lucidum strains in histo-anatomical traits of Ganoderma. Pore color of Korean G. lucidum strains was less brown than those of Taiwan and North American strains. Shapes of pores were round or circular in Korean strains but ellipsoidal or angular in Taiwan or North American strains. Pore numbers of Korean strains were more than those($4{\sim}6/mm$) of Taiwan or North American strains. Hardness of the pileus of Korean strains was much more than that of Taiwan or North American strains. Such characteristics of Korean strains were different from those of other species of Ganoderma. Korean G. lucidum strains could be classified into the other group because they had many different traits in growth characteristics of fruiting bodies and histo-anatomical characteristics from those of Taiwan or North American G. lucidum and other species of G. lucidum complex.

• 환경위생공학
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• 제18권2호
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• pp.42-51
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• 2003

Public awareness of air pollution problem has increased the need for real time air pollution information system about changes in air pollution level. The purpose of this study is to construct e-AIR, a user-friendly air pollution information system for urban air quality using GIS(geographic information system) technology for windows. GIS was ideally suited featuring a geographical characteristics(e.g., road, traffics, buildings) and very effectively used in mapping and symbolization for the distribution of the spatial/periodic pollution status(e.g., pie or column chart, graduated symbols) which can be effectively applied to a information system on the web-site. And a user interface, GUI(graphic user interface) was designed very diversely and simply enabled the users connect with e-AIR and obtain a useful information of air quality. A interpretive technique, air pollution health index(e.g., PSI, AEI) was used also which transforms complex data on measured atmospheric pollutant concentrations into a single number or set of numbers in order to make the data more understandable. Eventually the final-step of this study was to construct e-AIR based on Web GIS could be assessed anywhere if internet is available and offer a very useful information services of the air pollution to the public like a weather news.

• 대한기계학회논문집A
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• 제23권6호
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• pp.979-987
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• 1999

In a high precision vertical machining center, the estimation of cutting forces is important for many reasons such as prediction of chatter vibration, surface roughness and so on, and cutting forces are difficult to predict because they are very complex and time variant. In order to predict the cutting forces of end-milling process for various cutting conditions, a mathematical model is important and this model is based on chip load, cutting geometry, and the relationship between cutting forces and chip loads. Specific cutting force coefficients of the model have been obtained as interpolation function types by averaging farces of cutting tests. In this paper, the coefficients are obtained by neural network and the results of the conventional method and those of the proposed method are compared. The results show that the neural network method gives more correct values than the function type and that in teaming stage as the omitted numbers of experimental data increases the average errors increase.

• 오토저널
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• 제12권3호
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• pp.53-62
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• 1990

A wind tunnel experiment for the flow around a 1/5 scale passenger vehicle model has been carried out. A 5-hole Pitot tube is used for measuring velocity distributions around the model and a scanivalve with 48 ports is used for measuring surface pressure distribution at various Reynolds numbers. In order to observe the flow on the surface and in the wake region, a flow visualization experiment has been performed using wool tuft with and without paper cones. In addition, a 2-dimensional viscous calculation considering only the mid-plane section of the model has been performed. A complex wake structure in the immediate rear of the model has been confirmed. The distributions of the surface pressure coefficient are not sensitively dependent on the Reynolds Number. In the first half of the model, they do not seem to vary form section to section. However, in the second half, they do vary from section to section, especially at the bottom surface, which indicates that the cross flow vortex is more affected by the bottom surface than the top surface. The qualitative agreement of the measured and calculated velocity field also explains the usefulness of the 2-dimensional calculation in the limited sense.

• 대한한의학회지
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• 제20권2호
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• pp.128-140
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• 1999

To evaluate the antitumor activity, antimetastatic and immunomodulatory effects of samryongbakchulsankamibang(SBSK) studies were done experimentally, In cytotoxicity against P388, A549. SK-OV-3, B16-F10 and SK-MEL-2. concentration inhibiting cell growth up to below 40% of control was recognized at $10^{-3}g/ml$ of SBSK. In Inhibitory effect on activity of DNA topoisomerase I. the $IC_{50}$ was shown $200-400{\mu}g/ml$ of SBSK. The T/C was 154% in SBSK-treated group in S-180 bearing ICR mice, The concentration inhibiting adhesion of A549 and B16-F10 to complex extracellular matrix up to below 30% of control was recognized at $5{\times}10^{-4}$, $1{\times}10^{-3}\;g/ml$ of SBSK. In pumonary colonization assay with B16-BL/6, a number of colonies in the lungs were decreased significantly in SBSK-treated group as compared with control group, In hematological changes in B16-BL/6 injected C57BL/6, numbers of WBC and platelet were not changed significantly in SBSK-treated groups, In CAM and in vitro neovascularization assay, angiogenesis was inhibited significantly in SBSK-treated group as compared with control group. From above results it was concluded that SBSK could be usefully applied for the prevention and treatment of cancer.