• Journal of Microbiology
• /
• v.40 no.4
• /
• pp.274-281
• /
• 2002

The competitive quantitative PCR method targeting pcbC gene was developed for monitoring 4-chlorobiphenyl(4CB)-degrading bacteria, Pseudomonas sp. strain DJ-12, in soil microcosms. The method involves extraction of DNA from soil contaminated with 4CB, PCR amplification of a pcbC gene fragment from the introduced strain with a set of strain-specific primers, and quantification of the elec-trophoresed PCR product by densitometry. To test the adequacy of the method, Pseudomonas sp. strain DJ-12 was introduced into both contaminated and non-contaminated soil microcosms amended with 4CB. Pseudomonas sp. strain DJ-12 was monitored and quantified by a competitive quantitative PCR in comparison with 4CB degradation and the result was compared to those obtained by using the conventional cultivation method. We successfully detected and monitored 4CB-degrading bacteria in each microcosm and found a significant linear relationship between the number of 4CB-degrading bacteria and the capacity for 4CB biodegradation. The results of DNA spiking and cell-spreading experiments suggest that this competitive quantitative PCR method targeting the pcbC gene for monitoring 4CB- degrading bacteria appears to be rapid, sensitive and more suitable than the microbiological approach in estimating the capacity of 4CB biodegradation in environmental samples.

• Journal of Korean Society of Occupational and Environmental Hygiene
• /
• v.31 no.3
• /
• pp.274-285
• /
• 2021

Objectives: The question of whether the level of fees paid to working environment measurement agencies is appropriate has long been a matter of concern to the government. In addition, measurement institutions express dissatisfaction with their level of compensation, which has a great influence on the evaluation of a subject's policy. This study is intended to find a way to appropriately calculate working environment measurement fees. Methods: We looked at the principle of fee determination as a basic theory of fee calculation used in fee calculation, the legal and academic aspects of the general method of fee calculation, and government cost calculation standards. Furthermore, we reviewed the research methods applied so far to derive a method of calculating fees appropriate for this environment. Results: The working environment measurement environment is different from other commission calculation environments. The other environment is to appropriately calculate the service price provided by a monopoly public enterprise, while the situation is to appropriately calculate the fees provided by competitive private enterprises. Therefore, the service delivery environment and the delivery entity are different. In this case, the appropriate method of calculating service fees would be competitive pricing. There have also been many problems under the method of calculation by service cost. Conclusions: First, the working environment measurement fee requires an accounting correction of endogenous variables. Second, the theory of calculating fees appropriate for this situation is appropriate for competitive pricing that applies to private competitors. Third, the government should make efforts to make the service supply market a fully competitive market while ensuring that the service fee level is determined at the marginal cost level. Fourth, economically, research on marginal cost levels is needed.

• Proceedings of the Korean System Dynamics Society
• /
• 1999.08a
• /
• pp.105-132
• /
• 1999

If a system such as a port has a large boundary and complexity, and the system's substance is considered as a black box, forecast accuracy will be very low. Furthermore various components in a port exert significant influence on each other. To copy with these problem the form of structure models were introduced by using SD method. The Competitive Ports Model had several sub-systems consisting of each Unit Port models, and each Unit Port model was made by quantitative, qualitative factors and their feedback loops. The fact that all components of one port have influence on the components of the other ports should be taken into account to construct Competitive Port Models. However, with the current approach that is impossible, and in this paper, therefore, models were simplified by HFP adapted to integrate level variables of unit port models. Although many studies on modelling of port competitive situation have been conducted, both theoretical frame and methodology are still very weak. In this study, a new algorithm called ESD(Extensional System Dynamics) for the evaluation of port competition was presented, and applied to simulate port systems in northeast Asia.

• The Journal of Asian Finance, Economics and Business
• /
• v.8 no.3
• /
• pp.505-515
• /
• 2021

The essential purpose of this study is to propose strategies for developing micro-, small-, and medium-sized enterprises (MSMEs) to realize sustainable competitive advantage by applying the SWOT analysis method. Thus, the main problems investigated in this study are: a) around 60-70% of MSMEs in Kulonprogo regency do not yet have access or financing from banks; b) lack of knowledge of production technology; c) in general, MSMEs business actors are still incorporated legal entities; d) MSMEs do not have a good financial administration and management system; e) coordination between MSMEs stakeholders has not been integrated; f) limited facilities and infrastructure of MSMEs, primarily related to technological tools; and g) limited access to raw materials so that MSMEs often get low-quality raw materials. This study employed a survey method with questionnaires and interviews. By using the Slovin tools sampling technique, the number of samples was 39 MSMEs in Kulonprogo Regency, Yogyakarta, Indonesia. The results of this study confirmed that the Kulonprogo MSMEs should pay attention to seven aspects of business management to achieve sustainable competitive advantage. The seven aspects are: 1) business strategy; 2) human resources; 3) information technology; 4) products; 5) promotion; 6) cooperation; and 7) corporate social responsibility (CSR).

• The Journal of Asian Finance, Economics and Business
• /
• v.7 no.12
• /
• pp.329-336
• /
• 2020

This study aims to assess the impact of competitive pressure and innovation capability on business performance in small- and medium-sized enterprises (SMEs) in the batik industry in East Java, Indonesia. Furthermore, this study explores the impact of competitive pressure on business performance through innovation capability as a mediating variable. This research was quantitative using primary data with questionnaire as a method of sampling collection. The measurement of the variables was captured using Likert scale. The respondents were small- and medium-sized enterprises (SMEs) in the batik industry in East Java Province, Indonesia. The sample totaled 254 subjects. The data analysis was done using SEM-PLS. The results of the study show that: 1) there was a positive direct impact of competitive pressure on business performance; 2) competition pressure positively influences innovation capability; 3) innovation capability positively influences business performance; and 4) innovation capability has a partial mediating role in the effect of competitive pressures on business performance. The findings of this study suggest that managers in SME's batik industry should increase their effort to cope with the high competitive pressure to increase the innovation capability, so that they can have an advantage to face successfully competitors, leading to higher business performance.

• Microbiology and Biotechnology Letters
• /
• v.27 no.4
• /
• pp.344-348
• /
• 1999

A competitive enzyme-linked immunosorbent assay(ELISA) for the detection of antibodies to Treponema pallidum(T.pallidum) was developed and evaluated. T.apllidum lysate was immobilized on the surface of microplate wells and horseradish peroxidase labeled human anti-T.pallidum lysate was immobilized on the surface of microplate wells and horseradish peroxidase labeled human anti-T.pallidum was prepared and used as a tracer. The performance of the competitive ELISA was evaluated by using different specimens. The competitive ELISA showed a sensitivity of 100% in a performance panel consisting of serum and plasma with anti-T.pallidum reactivity ranging from negative to strong positive by FTA-ABS test system and 120 plasma samples positive by TPHA. The specificity of the competitive ELISA was 100% in 1,200 plasma samples collected from healthy seronegative blood donors. These results suggest that the competitive ELISA provides an excellent assay method for the detection of antibodies to T.pallidum, and may be particularly useful for serological blood screening of syphilis.

• The Journal of Asian Finance, Economics and Business
• /
• v.8 no.3
• /
• pp.237-249
• /
• 2021

Indonesia has successfully increased its ranking to 40th place in the 2019 Travel & Tourism Competitiveness Index. While tourism has become the country's second largest foreign exchange contributor, there is no existing competitive advantage model for Indonesian tourist destinations. The purpose and novelty of this study is to develop and formulate a competitive advantage model for Indonesia's tourism industry. The model will be based on the supply-side perception analysis of competitiveness indicators from Bali and five designated super-priority destinations in Indonesia. This model is expected to become a guideline for policymakers to design an effective and focused strategy. Data were obtained from in-depth interviews with, and questionnaires given to, 62 qualified industry players from the public and private sectors. This data-driven approach builds a relationship between competitiveness indicators and competitive advantages using a combination of importance-performance analysis and confirmatory factor analysis, thereby leveraging these advantages to generate a strategic model to compete in the international tourism industry. This would also be the first study to use this method in defining the competitive advantage of a destination. Using structural equation modeling, the study found that there are 54 indicators representing twelve dimensions of competitive advantages with good fit criteria.

• The Journal of Asian Finance, Economics and Business
• /
• v.8 no.4
• /
• pp.875-884
• /
• 2021

Competitive aggressiveness has long been believed to be the direct trigger for increased business performance, however, as a mediating variable it still needs to be further proven. This paper aims to examine the causal relationship between network capability, knowledge creation, innovativeness, competitive aggressiveness, and business performance of private universities. One model is proposed to test the role of competitive aggressiveness as a mediating variable. The population is lecturers at the 10 best private universities in East Java, Indonesia. Analysis by SEM, on 230 respondents, using random sampling method. The results show that the model is accepted, and competitive aggressiveness is proven to be a positive mediating variable in the relationship of network capability, knowledge creation, innovativeness, and business performance. The results also show that knowledge creation, and innovativeness, have an effect on competitive aggressiveness, while network capability has no effect. The research implication is that management should encourage lecturers and organizations to be more productive in conducting research and writing articles published in reputable journals, this will increase the ranking of universities. In order for the lecturers be more enthusiastic, the management gave an award to each lecturer who could submit their articles, which were then published by reputable journals.

• KSII Transactions on Internet and Information Systems (TIIS)
• /
• v.15 no.4
• /
• pp.1263-1274
• /
• 2021

In online competitive social networks, each user can be influenced by different competing influencers and consequently chooses different products. But their interest may change over time and may have swings between different products. The existing influence spreading models seldom take into account the time-related shifts. This paper proposes a minimum cost influence maximization algorithm based on the competitive transition probability. In the model, we set a one-dimensional vector for each node to record the probability that the node chooses each different competing influencer. In the process of propagation, the influence maximization on Competitive Linear Threshold (IMCLT) spreading model is proposed. This model does not determine by which competing influencer the node is activated, but sets different weights for all competing influencers. In the process of spreading, we select the seed nodes according to the cost function of each node, and evaluate the final influence based on the competitive transition probability. Experiments on different datasets show that the proposed minimum cost competitive influence maximization algorithm based on IMCLT spreading model has excellent performance compared with other methods, and the computational performance of the method is also reasonable.

• Journal of the Korean Geographical Society
• /
• v.46 no.3
• /
• pp.366-381
• /
• 2011

A competitive location problem in discrete space is computationally difficult to solve in general because of its combinatorial feature. In this paper, we address an alternative method for solving competitive location problems in discrete space, particularly employing deterministic allocation. The key point of the suggested method is to reducing the number of predefined potential facility sites associated with the size of problem by utilizing geometric concepts. The suggested method was applied to the existing broadband marketplace with increasing competition as an application. Specifically, we compared computational results and spatial configurations of two different sized problems: the problem with the original potential sites over the study area and the problem with the reduced potential sites extracted by a GIS-based geometric algorithm. The results show that the competitive location model with the reduced potential sites can be solved more efficiently, while both problems presented the same optimal locations maximizing customer capture.