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Studies on the Maturation of the Follicular Oocytes by Xenoplastical Transplantation in the Anterior Chamber of the Eye (異種動物의 眼前房에 이식된 濾胞卵子의 成熟에 관한 연구)

  • Cho, Wan Kyoo
    • The Korean Journal of Zoology
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    • v.13 no.3
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    • pp.68-74
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    • 1970
  • In the previous studies, the present author found that high proportion of the follicular oocytes from mouse and rabbit ovaries are able to resume their maturation division in the anterior chamber of the eye in which they have been incubated by auto- or homoplastic transplantation. Especially in the case of the homoplastic transplantation, it was known that no trouble has been detected in the process of resumption of the oval maturation in particular connection with the antigen-antibody reaction between donor and recipient. These findings provide a possibility that the follicular oocytes from various animals would be matured in the eye even after the xenoplastic transplantation. Under such an assumption, the present studies were performed to examine the behavior of the follicular oocytes in the eye chamber of the animals of different species. For the donor of the follicular oocytes, domestic rabbits, albino rats of Sprague-Dowley strain, and albino mice of A-strain bred in our laboratory were used. The oocytes obtained from the ovarian follicules were introduced to the anterior chamber of the eye of different species of animals, with an exception of rabbit in which only the female animals were used as a recipient. The procedures of collection of ova, introduction to the eye, harvest from the eye ball, fixation, and staining were the same as mentioned in the previous reports (Cho, 1967b; Cho and Kim, 1968). The conclusions obtained are summarized as below. 1. The rabbit follicular oocytes are able to mature in the eye chambers of both male mouse and rat, although the proportion of the maturation is lower than when they are incubated autoplastically in the eye. When the ova were incubated in the male mouse eye for 24 hours, 21 per cent of them showed chromosomes at metaphase I and II, whereas the rate was 32 per cent when they were incubated in the eye of the male rat. These are apparently low comparing to the rate of 52 per cent of autoplastic transplantation. 2. When rat follicular oocytes were transferred into the mouse eye chamber and recovered after 24 hours, 43 per cent of them produced the mataphase I and II chromosomes. This proportion was higher than the result of the homoplastic transplantation which yielded 23 per cent of the ova on maturation. 3. The most striking result was found in the experiment with mouse follicular oocytes. Seventy-six per cent of the oocytes resumed their maturation division within 24 hours after they were transferred into the male rat eye chamber, and this figure was significantly high compared to the result o 55 per cent obtained by the homoplastic transplantation. In the rat eye, the induction of the degenerative ova also was low (19%). On the other hand, the proportion of the oval maturation decreased to 45 per cent, while that of degeneration increased 33 per cent when they were incubated in the eye of the female rabbit. 4. It was apparent from the present experiments that the follicular oocytes can reveal their activation to maturation in the eye chamber which contains aqueous humor which is known to be composed of low protein content and of very little gamma-globulin which acts as an antibody(Oser, 1965), and that it shows higher osmolarity than blood serum(Levene, 1958). Taking these properities into consideration the humor may provide unfavourable environment to the cells and tissues incubated in. However, it could be noteworthy finding that only the follicular oocytes in the eye of the different species can grow in healthy condition although the maturation rates are varied with the animal species. The fact that the rabbit follicular oocytes show the lower proportion in maturation may be due to the greater amount of the yolk granules in the egg cytoplasm than those in the mouse and rat oocytes. That the mouse oocytes incubated in the eye of the rat mouse and rat oocytes. That the mouse oocytes incubated in the eye of the rat resumed their maturation process in greater proportion would e explained by the fact that the rat eye chamber particularly provides the better environment to the mouse oocytes than the eye chamber of mouse does.

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Effects of Combined Breathing Exercise and Neuromuscular Electrical Stimulation on Patients with Severe COPD (중증 만성폐쇄성폐질환 환자를 위한 복합호흡운동과 신경근전기자극의 효과)

  • Kang, Jeong-Il;Park, Jun-Su;Jeong, Dae-Keun
    • Journal of Korea Entertainment Industry Association
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    • v.13 no.7
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    • pp.539-548
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    • 2019
  • This study was designed to examine the effects of complex breathing exercise and neuromuscular electrical stimulation of Quadriceps Femoris muscle on pulmonary function and cerebral cortex activity in patients with severe chronic obstructive pulmonary disease. After collecting samples from 20 patients with severe chronic obstructive pulmonary disease aged 60 to 80, 10 patients each were randomly placed in an experimental group and a control group. The experimental group conducted complex breathing exercise and neuromuscular electrical stimulation of Quadriceps Femoris muscle, and the control group only conducted complex breathing exercise. As a pretest, pulmonary function and cerebral cortex activity were measured. The intervention program was applied to each group for 30 minutes, once a day, for 4 days a week, for 6 weeks, and the posttest was carried out the same way as the pretest. As a result, both groups showed significant differences in FEV1.0(Forced Expiratory Volume in One Second)(p<.001)(p<.05), and there were significant differences between the groups as well(p<.05). When comparing alpha waves in each domain of cerebral cortex, both of the experimental and control groups showed significant differences in Fp1, Fp2, F3 and F4 domains (p<.01)(p<.05). During the 6-week experiment, complex breathing exercise and neuromuscular electrical stimulation of Quadriceps Femoris muscle improved pulmonary function of patients with severe chronic obstructive pulmonary disease, and in relation to cerebral cortex activity, a positive breathing change was found due to the increase of alpha waves in the forehead domain. Therefore, it is considered that applying neuromuscular electrical stimulation of Quadriceps Femoris muscle to patients with severe chronic obstructive pulmonary disease additionally along with complex breathing exercise will bring a better therapeutic effect.

Comparative Analysis of the Poverty-Mitigating Effects Originated from Transfer Income Systems among Single-Elderly-Households (이전소득의 독거노인가구 빈곤경감 효과 비교)

  • Kim, Sooyoung;Lee, Kanghoon
    • 한국노년학
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    • v.29 no.4
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    • pp.1559-1575
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    • 2009
  • As the basic old-age pension system was enforced in 2008, the base for old-age income security was founded. However, due to the basic old-age pension played a minor role as assistant allowance, it did not reach to sufficient level to cover full income security system. It is estimated that the dependency on private transfer income among the elderly who are difficult to be economically independent is still high. Therefore the poverty rate of the elderly households, who are not economically active or who are not protected by old-age income security system, is more likely to be higher than that of non-elderly households. Based on the assumption that public transfer income system should become a central means of old-age life guarantee, this study examined the poverty mitigation effects among the elderly households by comparing the private transfer income and the public transfer income. For this purpose, we selected single-elderly-households who have been considered the most vulnerable to poverty. We used 2006- 2008 Household Income and Expenditure Survey dataset that contained single-elderly who were older than 65 years old. To understand the conditions of poverty among single-elderly-households and the degree of poverty-reducing effect originated from income transfer system, we compared the poverty rates of total households and the whole elderly households. Next, we analysed the poverty of the single-elderly-households by social demographic factors such as gender, age, and economic activity. Our major findings are as follows: First, the poverty rate of the whole elderly households were not reduced, even though the basic old-age pension and long-term care management system were enforced in 2008. Second, half of the elderly households including single-elderly-households belonged to the absolute poverty line. Relatively higher level of poverty among the single-elderly-households was found especially those who were female, unemployed, low-educated, older, and rural single-elderly-households. Third, the effect of the public transfer income on mitigating the single-elderly-households poverty showed a little progress. However, even greater poverty reducing effect was found by the private transfer income system. Fourth, in a group of the public transfer systems, the public assistance such as supporting living costs contributed more to reduce poverty of the elderly population than the public pension system did.

A Critical Study of the Legend on the Chinese Ancient Dynasty's Succession before Yao-Shun Era : Focusing on the Rongchengshi in the Shanghai Bowuguan zang Zhanguo Chuzhushu(II) (上海博物館蔵戦国楚竹書 《容成氏》 の古帝王帝位継承説話研究)

  • 李承律
    • Journal of the Daesoon Academy of Sciences
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    • v.17
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    • pp.197-225
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    • 2004
  • The respective history of the Chinese Ancient Dynasties from the era of the ancient Emperors to the revolutionary era of Yin-Zhou殷周 was described in the Rongchengshi容成氏, one of the texts in Shanghai Bowuguan zang Zhanguo Chuzhushu(II)上海博物館藏戰國楚竹書(二) discovered in 1994 at an antique market in Hong Kong. Drawn from the historical explanations expressed in it, the anonymous author's own views on history could be observed as largely being composed of 'resignation'禪讓, 'usurpation'簒奪, and 'banishment/smite'放伐. Following the advent of the recently excavated bamboo slips of Rongchengshi, a careful reconsideration is urgently needed to the established interpretation on the origin of the ritual of resignation in the relevant academic circles. Because it shows us that the ritual of resignation as a way of the succession, judging from my analysis, was already realized by Nine Emperors('Rongchengshi', Zunlushi尊盧氏, Hexushi赫胥氏, Gaoxinshi高辛氏, Cangjieshi倉頡氏, Xuanyuanshi軒轅氏, Shennongshi神農氏, 渾沌氏, and Baoxishi包羲氏 and maybe the more) before the era of 'Yao-Shun'堯舜. Accordingly, the aforementioned fact, which has never been elaborated in the previous texts including the first Chinese historiography Shiji史記, is the only peculiar feature to the Rongchengshi itself. Thus, a simple but empirically important question could be raised here: Was this way of description an exceptional case, even as unaccepted in Warring States Period at that time? If then, the Rongchengshi could not but help being evaluated merely as a buried historical texts, without any influence on the ancient Chinese, along with its author. The Chu bamboo slips Tangyu zhi dao唐虞之道 from Guodian Chujian郭店 excavated in 1993, however, has a very similar content to the Rongchengshi in relevance to the historical existence of the ritual of resignation. From the sentence, expressed in Tangyu zhi dao, that "the sudden rise of 'Six Emperors'六帝 was due to the practice of resignation like the period of Yao-Shun", it could be easily presumed that the 'Six Emperors' was closely connected to the 'Nine Emperors' and maybe the more at that time. Comparing with the related extant literary texts and the excavated materials in a vigorous way, in this paper, I explore four significant questions from a more critical stand to the conventional studies. First of all, I explicate the distinctiveness of the Rongchengshi as an academically very precious materials. Secondly, and closely related to the above, I evaluate its status or significance in the history of the Chinese ancient thoughts. And thirdly, I make an endeavor to trace back to the date of its transcription. Finally, and the most crucially, I attempt to show what Schools it was originated from and what connections it had with the Schools in the acient China. In sum, some concluding remarks, having somewhat insightful and significant implications for the further studies in these issues, could be drawn from my exploration. Viewing from the historical perspective of thoughts, at first, the legend of Ancient Dynasty's Succession before Yao-Shun Era in the Rongchengshi had some interactions directly and closely with Zhangzi莊子, Muzi墨子, Guanzi管子, Xunzi荀子 and Tangyu zhi dao. Also in doing search for the unification in a transitional epoch from the late to the end of the Warring States period, the political stand of Shi士 and Ke客 was reflected in it as well as in the Tangyu zhi dao because they actively wanted to suggest the most appropriate model of the Emperor or the idealistic succession process and political realms.

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The Study of Attributes of Immune Changes during the Convalescence Temperature Period in Holstein Dairy Cows Exposed to High-Temperature Stress (고온 스트레스 환경에 노출된 홀스타인종 젖소의 회복기 면역 변화 특성 규명)

  • Eun Tae Kim;Sangjin Lee;Ye Eun Kim;Dong-Hyun Lim;Dong Hyeon Kim;Seong Min Park;Jun Sik Eom;Ji Hoo Park;Sang Bum Kim;Sung Sill Lee;Myunghoo Kim
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.43 no.4
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    • pp.206-215
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    • 2023
  • This study was performed to investigate immune changes by comparing the proportion and function of immune cells in the blood under high-temperature period and convalescence temperature period in Holstein dairy cows. The experiment was conducted using Holstein dairy cows of five animals per group (60 ± 20 months old, 175 ± 78 non-day) from the National Institute of Animal Science at high-temperature period (THI: 76 ± 1.2) and convalescence temperature period (THI: 66 ± 1.3). Complete blood count results showed no change in the number of immune cells between groups. In the analysis using Flow Cytometry of PBMCs, no significant differences were observed among B cells, Helper T cells, cytotoxic T cells, and γδ T cells between groups. However, there was an increase in Th17 cells producing IL-17a, while Th1 cells decreased during the convalescence temperature period. The results of gene expression analysis using qRT-PCR in PBMCs revealed an increase in IL-10 during the convalescence temperature period, while a decrease in HSP70 and HSP90 was observed. In conclusion, the increased expression of IL-10 and the decrease in HSP expression suggest the possibility of a weak recovery from heat stress. However, the lack of observed changes in B cells, T cells, and other immune cells indicates incomplete recovery from heat stress during the convalescence temperature period.

The Study on the Class Difficulty of Elementary Pre-service Teachers' Seasonal Change Unit (초등예비교사의 계절변화 단원에 대한 수업곤란도 연구)

  • Soon-shik Kim
    • Journal of the Korean Society of Earth Science Education
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    • v.16 no.3
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    • pp.340-350
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    • 2023
  • This study analyzed the difficulty level of class on the seasonal change unit for 84 students at a university of education. The conclusions of this study are as follows. First, if we first present the four topics that make up the seasonal changes in elementary science, the subjects that have the greatest difficulty in teaching for prospective elementary school teachers are 'Why do seasonal changes occur?' (Teaching difficulty level 4.05), 'The sun changes depending on the season' What is the difference between the southern altitude and the length of day and night?' (difficulty level of class, 3.12), 'What is the relationship between the altitude of the sun, length of shadow, and temperature during the day?' (difficulty level of class, 2.85), 'How does the temperature change depending on the season?' (class difficulty level 2.80). As a result, in the elementary science season change unit, the class on the four topics 'Why do seasons change?', which is classified as a class topic that requires the concept of spatial perception, showed a higher level of class difficulty than other units. Second, in the seasonal change unit, various factors of class difficulty appeared depending on the class topic. When pre-service elementary school teachers look at the factors that make class difficult when teaching a lesson on seasonal changes in order of frequency, 42 (50%) said 'Experimental instruction for comparing the altitude of solar masculine according to the tilt of the axis of rotation', followed by 'Solar masculine'. 38 people (45%) answered 'Difficulty in explaining mid-high altitude and the length of day and night', 27 people (32%) answered 'Difficulty in explaining the concept of mid-high altitude', and 24 people (32%) answered 'Difficulty in explaining seasonal changes in the sun's position.' 29%), 20 people (24%) said 'Explain the reasonable reason why the height of the light should be adjusted when measuring the solar altitude', and 16 people (19%) said 'It is difficult to explain the reason for the discrepancy between the solar altitude and the maximum temperature'. ), 'difficulties in measuring sand (ground) temperature' were mentioned by 12 people (14%). Third, when analyzing the factors of class difficulty, there were more curriculum factors than teacher factors. In this context, the exploratory activities on 'Why do seasonal changes occur?', the fourth topic of the seasonal change unit in which elementary school pre-service teachers showed the greatest difficulty in teaching, need improvement in terms of the curriculum.

Estimation of Rice Canopy Height Using Terrestrial Laser Scanner (레이저 스캐너를 이용한 벼 군락 초장 추정)

  • Dongwon Kwon;Wan-Gyu Sang;Sungyul Chang;Woo-jin Im;Hyeok-jin Bak;Ji-hyeon Lee;Jung-Il Cho
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.25 no.4
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    • pp.387-397
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    • 2023
  • Plant height is a growth parameter that provides visible insights into the plant's growth status and has a high correlation with yield, so it is widely used in crop breeding and cultivation research. Investigation of the growth characteristics of crops such as plant height has generally been conducted directly by humans using a ruler, but with the recent development of sensing and image analysis technology, research is being attempted to digitally convert growth measurement technology to efficiently investigate crop growth. In this study, the canopy height of rice grown at various nitrogen fertilization levels was measured using a laser scanner capable of precise measurement over a wide range, and a comparative analysis was performed with the actual plant height. As a result of comparing the point cloud data collected with a laser scanner and the actual plant height, it was confirmed that the estimated plant height measured based on the average height of the top 1% points showed the highest correlation with the actual plant height (R2 = 0.93, RMSE = 2.73). Based on this, a linear regression equation was derived and used to convert the canopy height measured with a laser scanner to the actual plant height. The rice growth curve drawn by combining the actual and estimated plant height collected by various nitrogen fertilization conditions and growth period shows that the laser scanner-based canopy height measurement technology can be effectively utilized for assessing the plant height and growth of rice. In the future, 3D images derived from laser scanners are expected to be applicable to crop biomass estimation, plant shape analysis, etc., and can be used as a technology for digital conversion of conventional crop growth assessment methods.

Taste Compounds and Antioxidant Properties in Extracts of Angelica keiskei and Oenanthe javanica Juice By-Products According to Extraction Methods (추출 방법에 따른 명일엽과 돌미나리 착즙박의 정미성분 및 항산화 특성)

  • Hyun Jung Lee;Ha Na Ryoo;Hyeon Gyu Lee
    • Journal of Food Hygiene and Safety
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    • v.38 no.6
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    • pp.517-527
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    • 2023
  • This study aimed to examine the possibility of upcycling extracts of Angelica keiskei and Oenanthe javanica juice by-products through comparing enzyme extraction (EE) and complex extraction (CE) methods to increase the extraction yield and flavor of materials. A higher extraction yield was obtained for free amino acid content with EE and CE for A. keiskei and O. javanica juice by-products, respectively, and a higher extraction efficiency was achieved with juice by-products than with extracts prepared from raw materials before juice production. The content of major amino acids varied depending on the extraction method used. When used according to the characteristics of the extract, their use as a functional material was confirmed along with improvement in the flavor of the food. Consistently high extraction yields for organic acid and sugar levels were obtained with CE in A. keiskei and O. javanica juice by-products. The DPPH radical scavenging ability and TPC were consistently high with CE in A. keiskei and O. javanica juice by-products; the increase in extracted content was likely because of the reaction between the ethanol used for CE and the phenolic compounds. However, because the antioxidant capacity of the juice by-product extracts was somewhat lower than that of the extracts from raw materials before juice production, the amount used should be reviewed. The TFC was found to be higher in extracts obtained with EE than with CE for A. keiskei juice by-products; however, no significant difference was observed between EE and CE in the O. javanica juice by-products. Through this study, the taste compounds and antioxidant properties of extracts obtained from juice by-products produced after the production of A. keiskei and O. javanica green juice were analyzed, and the availability of high value-added materials was confirmed. Based on these research results, expanding specific R&D for practical use should be explored.

Terrain Shadow Detection in Satellite Images of the Korean Peninsula Using a Hill-Shade Algorithm (음영기복 알고리즘을 활용한 한반도 촬영 위성영상에서의 지형그림자 탐지)

  • Hyeong-Gyu Kim;Joongbin Lim;Kyoung-Min Kim;Myoungsoo Won;Taejung Kim
    • Korean Journal of Remote Sensing
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    • v.39 no.5_1
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    • pp.637-654
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    • 2023
  • In recent years, the number of users has been increasing with the rapid development of earth observation satellites. In response, the Committee on Earth Observation Satellites (CEOS) has been striving to provide user-friendly satellite images by introducing the concept of Analysis Ready Data (ARD) and defining its requirements as CEOS ARD for Land (CARD4L). In ARD, a mask called an Unusable Data Mask (UDM), identifying unnecessary pixels for land analysis, should be provided with a satellite image. UDMs include clouds, cloud shadows, terrain shadows, etc. Terrain shadows are generated in mountainous terrain with large terrain relief, and these areas cause errors in analysis due to their low radiation intensity. previous research on terrain shadow detection focused on detecting terrain shadow pixels to correct terrain shadows. However, this should be replaced by the terrain correction method. Therefore, there is a need to expand the purpose of terrain shadow detection. In this study, to utilize CAS500-4 for forest and agriculture analysis, we extended the scope of the terrain shadow detection to shaded areas. This paper aims to analyze the potential for terrain shadow detection to make a terrain shadow mask for South and North Korea. To detect terrain shadows, we used a Hill-shade algorithm that utilizes the position of the sun and a surface's derivatives, such as slope and aspect. Using RapidEye images with a spatial resolution of 5 meters and Sentinel-2 images with a spatial resolution of 10 meters over the Korean Peninsula, the optimal threshold for shadow determination was confirmed by comparing them with the ground truth. The optimal threshold was used to perform terrain shadow detection, and the results were analyzed. As a qualitative result, it was confirmed that the shape was similar to the ground truth as a whole. In addition, it was confirmed that most of the F1 scores were between 0.8 and 0.94 for all images tested. Based on the results of this study, it was confirmed that automatic terrain shadow detection was well performed throughout the Korean Peninsula.

Estimation of Chlorophyll Contents in Pear Tree Using Unmanned AerialVehicle-Based-Hyperspectral Imagery (무인기 기반 초분광영상을 이용한 배나무 엽록소 함량 추정)

  • Ye Seong Kang;Ki Su Park;Eun Li Kim;Jong Chan Jeong;Chan Seok Ryu;Jung Gun Cho
    • Korean Journal of Remote Sensing
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    • v.39 no.5_1
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    • pp.669-681
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    • 2023
  • Studies have tried to apply remote sensing technology, a non-destructive survey method, instead of the existing destructive survey, which requires relatively large labor input and a long time to estimate chlorophyll content, which is an important indicator for evaluating the growth of fruit trees. This study was conducted to non-destructively evaluate the chlorophyll content of pear tree leaves using unmanned aerial vehicle-based hyperspectral imagery for two years(2021, 2022). The reflectance of the single bands of the pear tree canopy extracted through image processing was band rationed to minimize unstable radiation effects depending on time changes. The estimation (calibration and validation) models were developed using machine learning algorithms of elastic-net, k-nearest neighbors(KNN), and support vector machine with band ratios as input variables. By comparing the performance of estimation models based on full band ratios, key band ratios that are advantageous for reducing computational costs and improving reproducibility were selected. As a result, for all machine learning models, when calibration of coefficient of determination (R2)≥0.67, root mean squared error (RMSE)≤1.22 ㎍/cm2, relative error (RE)≤17.9% and validation of R2≥0.56, RMSE≤1.41 ㎍/cm2, RE≤20.7% using full band ratios were compared, four key band ratios were selected. There was relatively no significant difference in validation performance between machine learning models. Therefore, the KNN model with the highest calibration performance was used as the standard, and its key band ratios were 710/714, 718/722, 754/758, and 758/762 nm. The performance of calibration showed R2=0.80, RMSE=0.94 ㎍/cm2, RE=13.9%, and validation showed R2=0.57, RMSE=1.40 ㎍/cm2, RE=20.5%. Although the performance results based on validation were not sufficient to estimate the chlorophyll content of pear tree leaves, it is meaningful that key band ratios were selected as a standard for future research. To improve estimation performance, it is necessary to continuously secure additional datasets and improve the estimation model by reproducing it in actual orchards. In future research, it is necessary to continuously secure additional datasets to improve estimation performance, verify the reliability of the selected key band ratios, and upgrade the estimation model to be reproducible in actual orchards.