• Title/Summary/Keyword: Comparative calibration

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HPLC Validation of Valerian Ethanol Extract as a Functional Food (건강기능식품 원료로서 길초근 주정 추출물의 지표성분 분석법 검증)

  • Jo, Kyungae;Han, Sung Hee;Bae, Song-Hwan;Shin, Jung Cheul;Suh, Hyung Joo
    • The Korean Journal of Food And Nutrition
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    • v.31 no.1
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    • pp.104-108
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    • 2018
  • The purpose of this study was to establish valerenic acid as a marker compound for the standardization of ethanol extract of Valerinan officinalis (valerian) root as a functional health food. We established valerenic acid as a marker compound using HPLC. HPLC was used to quantify the marker compound in the valerian extract after validation of methods with linearity, accuracy, and precision. The specificity for retention time was met by comparative analysis of the valerian extract and standard compound using HPLC. The method showed high linearity of the calibration curve with a coefficient of correlation ($R^2$) of 0.9999. The limit of quantification (LOQ) was $10{\mu}g/mL$. The accuracy of measurement was 99.88~00.68% and the relative standard deviation (RSD) value was 0.59%. In addition, our analytical method yielded a 29% mean content of valerenic acid in the valerian ethanol extract. These results indicate that the established HPLC method facilitated the determination of marker compounds in the valerian extract for the standardization of health functional foods.

Comparative phenolic compound profiles and antioxidative activity of the fruit, leaves, and roots of Korean ginseng (Panax ginseng Meyer) according to cultivation years

  • Chung, Ill-Min;Lim, Ju-Jin;Ahn, Mun-Seob;Jeong, Haet-Nim;An, Tae-Jin;Kim, Seung-Hyun
    • Journal of Ginseng Research
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    • v.40 no.1
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    • pp.68-75
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    • 2016
  • Background: The study of phenolic compounds profiles and antioxidative activity in ginseng fruit, leaves, and roots with respect to cultivation years, and has been little reported to date. Hence, this study examined the phenolic compounds profiles and 2, 2-diphenyl-1-picrylhydrazyl (DPPH) free-radical-scavenging activities in the fruit, leaves, and roots of Korean ginseng (Panax ginseng Meyer) as a function of cultivation year. Methods: Profiling of 23 phenolic compounds in ginseng fruit, leaves, and roots was investigated using ultra-high performance liquid chromatography with the external calibration method. Antioxidative activity of ginseng fruit, leaves, and roots were evaluated using the method of DPPH free-radical-scavenging activity. Results: The total phenol content in ginseng fruit and leaves was higher than in ginseng roots (p < 0.05), and the phenol content in the ginseng samples was significantly correlated to the DPPH free-radical-scavenging activity ($r=0.928^{****}$). In particular, p-coumaric acid ($r=0.847^{****}$) and ferulic acid ($r=0.742^{****}$) greatly affected the DPPH activity. Among the 23 phenolic compounds studied, phenolic acids were more abundant in ginseng fruit, leaves, and roots than the flavonoids and other compounds (p < 0.05). In particular, chlorogenic acid, gentisic acid, p- and m-coumaric acid, and rutin were the major phenolic compounds in 3e6-yr-old ginseng fruit, leaves, and roots. Conclusion: This study provides basic information about the antioxidative activity and phenolic compounds profiles in fruit, leaves, and roots of Korean ginseng with cultivation years. This information is potentially useful to ginseng growers and industries involved in the production of high-quality and nutritional ginseng products.

A Preliminary Study on Polyester Aluminum Bag as the Possible Substitute for Tedlar Bag Sampler in RSC Analysis (테들러 백 샘플러의 대체 소재로서 폴리에스터 알루미늄 백에 대한 예비연구: 환원황화합물을 중심으로)

  • Kim, Ki-Hyun;Jo, Sang-Hee
    • Journal of Korean Society for Atmospheric Environment
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    • v.27 no.4
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    • pp.454-459
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    • 2011
  • In this study, the recovery rate of Tedlar bag (T) sampler was investigated in comparison to polyester aluminum bag (P) sampler. To derive the comparative data sets for the relative performance between different samplers, a series of calibration experiments were performed by using 1 ppb standard of four offensive reduced sulfur compounds (RSC) odorants ($H_2S$, $CH_3SH$, DMS, and DMDS) along with $SO_2$ and $CS_2$. All the analysis was made by gas chromatography/pulsed flame photometric detector (GC/PFPD) combined with air server/thermal desorber (AS/TD). The measurement data were obtained by loading gaseous standards (1 ppb) at 3 injection volumes (250, 500 and 1,000 mL) at three intervals (0, 24 and 72 hrs). The recovery rates (RR) of P sampler were computed against the slope values of T sampler. According to our analysis, P sampler exhibits slightly enhanced loss relative to T, especially with light RSCs ($H_2S$ and $CH_3SH$). At day 0, RR for the two were 88 and 85%, respectively. Such reduction proceeded rather rapidly in the case of $H_2S$ through time. However, P sampler was more stable to store $SO_2$ unlike others. Despite slightly reduced recovery, P sampler appears as a good replacement of T sampler.

The comparative study of PKNU2 Image and Aerial photo & satellite image

  • Lee, Chang-Hun;Choi, Chul-Uong;Kim, Ho-Yong;Jung, Hei-Chul
    • Proceedings of the KSRS Conference
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    • 2003.11a
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    • pp.453-454
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    • 2003
  • Most research materials (data), which are used for the study of digital mapping and digital elevation model (DEM) in the field of Remote Sensing and Aerial Photogrammetry are aerial photographs and satellite images. Additionally, they are also used for National land mapping, National land management, environment management, military purposes, resource exploration and Earth surface analysis etc. Although aerial photographs have high resolution, the data, which they contain, are not used for environment exploration that requires continuous observation because of problems caused by its coastline, as well as single - spectral and long-term periodic image. In addition to this, they are difficult to interpret precisely because Satellite Images are influenced by atmospheric phenomena at the time of photographing, and have by far much lower resolution than existing aerial photographs, while they have a great practical usability because they are mulitispectral images. The PKNU 2 is an aerial photographing system that is made to compensate with the weak points of existing aerial photograph and satellite images. It is able to take pictures of very high resolution using a color digital camera with 6 million pixels and a color infrared camera, and can take perpendicular photographs because PKNU 2 system has equipment that makes the cameras stay level. Moreover, it is very cheap to take pictures by using super light aircraft as a platform. It has much higher resolution than exiting aerial photographs and satellite images because it flies at a low altitude about 800m. The PKNU 2 can obtain multispectral images of visible to near infrared band so that it is good to manage environment and to make a classified diagram of vegetation.

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Comparative Study on Various Ductile Fracture Models for Marine Structural Steel EH36

  • Park, Sung-Ju;Lee, Kangsu;Cerik, Burak Can;Choung, Joonmo
    • Journal of Ocean Engineering and Technology
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    • v.33 no.3
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    • pp.259-271
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    • 2019
  • It is important to obtain reasonable predictions of the extent of the damage during maritime accidents such as ship collisions and groundings. Many fracture models based on different mechanical backgrounds have been proposed and can be used to estimate the extent of damage involving ductile fracture. The goal of this study was to compare the damage extents provided by some selected fracture models. Instead of performing a new series of material constant calibration tests, the fracture test results for the ship building steel EH36 obtained by Park et al. (2019) were used which included specimens with different geometries such as central hole, pure shear, and notched tensile specimens. The test results were compared with seven ductile fracture surfaces: Johnson-Cook, Cockcroft-Latham-Oh, Bai-Wierzbicki, Modified Mohr-Coulomb, Lou-Huh, Maximum shear stress, and Hosford-Coulomb. The linear damage accumulation law was applied to consider the effect of the loading path on each fracture surface. The Swift-Voce combined constitutive model was used to accurately define the flow stress in a large strain region. The reliability of these simulations was verified by the good agreement between the axial tension force elongation relations captured from the tests and simulations without fracture assignment. The material constants corresponding to each fracture surface were calibrated using an optimization technique with the minimized object function of the residual sum of errors between the simulated and predicted stress triaxiality and load angle parameter values to fracture initiation. The reliabilities of the calibrated material constants of B-W, MMC, L-H, and HC were the best, whereas there was a high residual sum of errors in the case of the MMS, C-L-O, and J-C models. The most accurate fracture predictions for the fracture specimens were made by the B-W, MMC, L-H, and HC models.

Design of Sub-array Receiver for Active Phase Array Radar (능동위상배열 레이더 부배열 수신기 설계)

  • Yi, Hui-min;Kim, Do-hoon;Han, Il-tak
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.23 no.5
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    • pp.568-573
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    • 2019
  • Modern Radars are evolving into MFRs which can search multiple targets simultaneously and then track them. Additionally they should be able to avoid some external jamming signals. Applying to these MFRs, Antennas should be able to perform DBF including to not only real-time beam steering but also multi-beam forming simultaneously. And they can cancel the beam at the specific direction. In this paper, we describe the implementation of sub-array type antenna hardware which can be applying DBF. Also we propose the modified amplitude aperture distribution for suppressing the side lobe level and explain the sub-array receiver design with amplitude tapering. It consists in making the amplitude weighting in 2 steps. In order to compare two weighting cases, we investigate the G/T performance for the array antenna. At the conclusion, we make a comparative study for the dynamic range of every sub-array receiver and present the hardware implementation that is more advantageous for sub-array alignment and calibration in DBF.

Water Budget Assessment for Soybean Grown in Paddy Fields Converted to Uplands Using APEX Model (APEX 모델을 이용한 콩 재배 밭 전환 논의 물수지 특성 평가)

  • Choi, Soon-Kun;Jeong, Jaehak;Yeob, So-Jin;Kim, Myung-Hyun;Kim, Min-Kyeong
    • Journal of The Korean Society of Agricultural Engineers
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    • v.63 no.4
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    • pp.55-64
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    • 2021
  • The expansion of upland crop cultivation in rice paddy fields is recommended by the Korean government to solve the problem of falling rice price and reduction of rice farmer's income due to oversupply of rice. However, water use efficiency is significantly influenced by the land use change from paddy field to upland. Therefore, this study aimed to evaluate the water budget of soybean grown in using APEX (Agricultural Policy and Environmental eXtender) model. The amount of runoff was measured in a test bed located in Iksan, Jeollabu-do and used to calibrate and validate the simulated runoff by APEX model. From 2019 to 2020, the water budget of soybean grown in uplands were estimated and compared with the one grown in paddy fields. The calibration result of AP EX model for runoff showed that R2 (Coefficient of determination) and NSE (Nash-Sutcliffe efficiency) were 0.90 and 0.89, respectively. In addition, the validated results of R2 and NSE were 0.81 and 0.62, respectively. The comparative study of each component in water budget showed that the amounts of evapotranspiration and percolation estimated by APEX model were 549.1 mm and 375.8mm, respectively. The direct runoff amount from upland was 390.1 mm, which was less than that from paddy fields. The average amount of irrigation water was 28.7 mm, which was very small compared to the one from paddy fields.

Dyeability of Nylon Fabrics with Dyestuff for Supercritical Fluid Dyeing (1) : C.I. Disperse Red 167, C.I. Disperse Violet 93 (초임계 유체 염색용 염료에 따른 Nylon 섬유의 염색 특성 (1) : C.I. Disperse Red 167, C.I. Disperse Violet 93 Azo계 염료)

  • Choi, Hyunseuk;Park, Shin;Kim, Taeyoung
    • Textile Coloration and Finishing
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    • v.32 no.4
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    • pp.217-225
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    • 2020
  • In this study, the dyeing characteristics of nylon fabric which is dyed with supercritical fluid were investigated. There were two dyes used in the dyeing experiment: C.I. Disperse Red 167 and C.I. Disperse Violet 93. Dyeing temperature, pressure, and leveling time were fixed at 110℃, 250bar, 60minutes, and the experiment was conducted with dyeing concentration of 0.1, 0.3, 0.5, and 0.85% o.w.f. The analysis of the experimental results was found out through the measurement of washing fastness and color coordinate. In addition, the calibration curve of each dye was drawn up and the amount of remaining dye was checked by measuring the absorbance of the residual dye. As a result of color difference measurement, as the concentration increased, the L⁎ value decreased and the K/S value increased. However, the increase in K/S value compared to the amount of input decreased as the concentration increased. The comparative experiment on the amount of residual dye(C.I. Disperse Red 167) in the pot showed that 99.14% of the amount was dyed at the concentration of 0.1% o.w.f, while it rapidly decreased to 77% at 0.85% o.w.f. C.I. Disperse Violet 93 dye also decreased from 0.5% o.w.f to 93.91%. In the washing fastness experiment of both dyes, the level of washing fastness began to decrease from samples dyed at 0.5% o.w.f. It may be because the simply absorbed dye was produced instead of completely being fixed in the amorphous region of the nylon fiber.

Low-Cost IoT Sensors for Flow Measurement in Open Channels: A Comparative Study of Laboratory and Field Performance

  • Khatatbeh, Arwa;Kim, Young-Oh
    • Proceedings of the Korea Water Resources Association Conference
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    • 2023.05a
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    • pp.172-172
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    • 2023
  • The use of low-cost IoT sensors for flow measurement in open channels has gained significant attention due to their potential to provide continuous and real-time data at a low cost. However, the accuracy and reliability of these sensors in real-world scenarios are not well understood. This study aims to compare the performance of low-cost IoT sensors in the laboratory and real-world conditions to evaluate their accuracy and reliability. Firstly, a low-cost IoT sensor was integrated with an IoT platform to acquire real-time flow rate data. The IoT sensors were calibrated in the laboratory environment to optimize their accuracy, including different types of low-cost IoT sensors (HC-SR04 ultrasonic sensor & YF-S201 sensor) using an open channel prototype. After calibration, the IoT sensors were then applied to a real-world case study in the Dorim-cheon stream, where they were compared to traditional flow measurement methods to evaluate their accuracy.The results showed that the low-cost IoT sensors provided accurate and reliable flow rate data under laboratory conditions, with an error range of less than 5%. However, when applied to the real-world case study, the accuracy of the IoT sensors decreased, which could be attributed to several factors such as the effects of water turbulence, sensor drift, and environmental factors. Overall, this study highlights the potential of low-cost IoT sensors for flow measurement in open channels and provides insights into their limitations and challenges in real-world scenarios.

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Comparative Study of the Efficiency of GC with Large Volume Injector and SPE Clean-up Process Applied in QuEChERS Method (GC-대용량 주입장치와 SPE를 적용한 QuEChERS 잔류농약 분석법의 효율성 비교)

  • Park, Young Jun;Hong, Su Myeong;Kim, Taek Kyum;Kwon, Hye Young;Hur, Jang Hyun
    • The Korean Journal of Pesticide Science
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    • v.19 no.4
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    • pp.370-393
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    • 2015
  • This study was conducted to compare STQ method, multi-residue method in Korean food code and QuEChERS method for validated selected and accuracy, reproducibility and efficiency. A total of 45 selected and targeted pesticides were the analyzed by GC and 5 of them were crops (apple, potato, green pepper, rice, soy bean). $R^2$ values were calculated in the standard calibration curve was over 0.990. Recovery tests were performed by three replications in two levels and the relative standard deviation of the repeated experiments was less than 30%. The average percentage of recoveries in the multi-residue method in Korean food code was 89.13%, QuEChERS method was 92.45% and STQ method was 85.28%. In addition, matrix effects in multi-residue method in Korean food code was 24.61%, QuEChERS method was 23.98% and STQ method showed 11.24%. The STQ method is easy and showed high clean-up effect in extracting the sample solution than the QuEChERS method and clean-up with C18, PLS, PSA cartridge columns. A large volume of the sample was injected in order to compensable for the problem, that occurred due to high detection limit in the analyser. When the STQ method was applied using a large volume injector, the standard calibration curve showed a higher linearity $R^2=0.990$, and method detection limit was 0.01 mg/kg. It showed an average recovery of 91.84% and the relative standard deviations of three replications repeated in two level process was less than 30% and had an average matrix effect of 17.90%.