• 제목/요약/키워드: Companion Animal

검색결과 373건 처리시간 0.02초

재조합 리보솜 단백질 L7/L12을 이용한 개 브루셀라병의 진단 (Diagnosis of canine brucellosis using recombinant ribosomal protein L7/L12)

  • 이향근;김종완;하윤미;허문;김지연;이기찬;강성일;정석찬
    • 대한수의학회지
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    • 제52권1호
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    • pp.25-31
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    • 2012
  • Brucella (B.) canis is mainly transmitted by direct or indirect contact with aborted fetuses and placenta. It's also known to be able to infect human, which likely results in providing veterinarians and companion animal owners for infectious risk. To develop diagnostic ELISA, we cloned and expressed rp1L gene of B. canis, which encodes the ribosomal protein L7/L12. Using this purified recombinant protein, indirect-ELISA (iELISA) was evaluated using 78 positive and 44 negative sera. The sensitivity and the specificity of iELISA were 94% and 89%, respectively. The results indicated that indirect-ELISA using recombinant ribosomal protein L7/L12 may be useful for diagnosis of canine brucellosis.

인터넷을 통한 반려견 식품 구매자의 인식 조사 (Awareness Survey of Consumers buying Companion Dog Food through Internet)

  • 손은심
    • 한국산학기술학회논문지
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    • 제21권6호
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    • pp.574-583
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    • 2020
  • 본 연구는 인터넷을 통하여 반려견 식품을 구매하는 소비자의 반려견을 위한 지출 품목 및 비용, 반려견 푸드 선택 시 고려사항 등을 조사하였다. 성별로는 남성 39명(6.8%) 여성 532명(93.2%)으로 반려견 식품을 구입할 때 대부분 여성들이 인터넷 구매를 한다는 것을 알 수 있었다. 견주 연령대로는 20대 이하 184명(32.2%), 30대 241명(42.2%), 40대 이상 146명(25.6%)로 나타났다. 반려견의 연령을 보면 3세 이하가 200(35%)이고, 8세 이상 202(35.4%), 4~7세 169(29.6%)으로 나타났다. 반려견을 위한 구매빈도 주기는 1달 이상 주기로 구매한다고 가장 많이 답하였으며, 구매 식재료 중에서는 닭고기를 가장 많이 선호하는 것으로 나타났다. 월지출비용에서도 10만원 미만이 가장 높게 나타났으며, 반려견 푸드 주요 구입처로는 반려견 전문 온라인 쇼핑몰, 그 다음으로는 동물병원에서 구입한다고 하였다. 건강기능 향상을 위해서는 피부·모질개선을 가장 중요하게 여기고 있으며 그 다음으로 치아 뼈 형성, 소화력 개선 순으로 나타났다. 반려견 푸드 선택 시 전제품의 영양성분을 가장 고려할 사항이라고 여겼으며 그 다음으로는 인지도가 있는 브랜드 제품, 색·냄새·형상, 기능개선제품, 제품의 가격 순으로 나타났다. 따라서 반려견 푸드 신제품을 개발할 때는 전제품의 영양성분 뿐만 아니라 브랜드 제품, 색·냄새·형상, 영양밸런스, 기능개선제품, 제품의 가격 등을 고려해야 할 것으로 보여진다.

편측 앞다리 파행이 있는 개에서 카프로펜 투여와 재활 중재의 효과 (Efficacy of carprofen administration and rehabilitative intervention in a dog with unilateral forelimb lameness)

  • 이신호;김수진;조재현
    • 한국동물위생학회지
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    • 제47권3호
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    • pp.185-191
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    • 2024
  • This case report examines the efficacy of carprofen and rehabilitation therapy in a 7-year-old, 4.5 kg, spayed Poodle with unilateral forelimb lameness. Gait analysis assessed kinetic parameters such as peak vertical force (PVF), vertical impulse (VI), symmetry index (SI), and body load distribution (BLD) before and after treatment. The differences between the left and right forelimbs were recorded. Before intervention, the PVF differences between the forelimbs were -4.00% during walking and 40.00% during trotting. Two weeks post-intervention, these improved to 8.33% and 27.67%, further reducing to 6.33% and 18.00% after four weeks. VI differences also improved, with initial values of -0.67 N∙s during walking and -1.3 N∙s during trotting decreasing to -0.03 N∙s and -0.87 N∙s, respectively. SI increased from 6.23% to 11.87% during walking, while it decreased from 33.63% to 14.73% during trotting. BLD differences reduced from -1.17 cm2 and -7.13 cm2 to 0.23 cm2 and -2.17 cm2, respectively, after four weeks. These results suggest that a combination of carprofen administration and rehabilitative intervention can effectively reduce lameness and improve symmetry in dogs with unilateral forelimb lameness, as evidenced by improvements in key gait parameters over the treatment period.

Comparison of the pathogenicity among Cronobacter species in a neonatal mouse model

  • Hong, Sun-Hwa;Chung, Yung-Ho;Park, Sang-Ho;Kim, Ok-Jin
    • 한국동물위생학회지
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    • 제36권2호
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    • pp.67-71
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    • 2013
  • Neonatal infection caused by Cronobacter species can result in serious illnesses such as bacteremia, septicemia, meningitis, and death in at-risk infants who are orally fed contaminated reconstituted powdered infant formulas. The objective of this study was to compare the virulence among three Cronobacter species strains by using an animal model for human neonatal Cronobacter species infections. We acquired timed-pregnant ICR mice and all owed them to give birth naturally. On postnatal day 3, each pup was administered orally a total dose of $1{\times}10^7$ CFU Cronobacter species strain 3439, CDC 1123-79, and 3231. Mice were observed twice daily for morbidity and mortality. At postnatal day 10, the remaining pups were euthanized, and brain, liver, and cecum were excised and analyzed for the presence of Cronobacter species. Cronobacter species were isolated from cecum and other tissues in inoculated mice. In the tissues of Cronobacter species infected mice, meningitis and gliosis were detected in the brain. In this study, we identified the virulence among Cronobacter species strains by using a neonatal mice model which was a very effective animal model for human neonatal Cronobacter species infections.

In vitro and in vivo inhibition of Helicobacter pylori by Lactobacilllus paracasei HP7

  • Hong, Seong-Soo;Lee, Hyun-A;Kim, Joo Yun;Jeong, Ji-Woong;Shim, Jae-Jung;Lee, Jung Lyoul;Sim, Jae-Hun;Chung, Yungho;Kim, Okjin
    • Laboraroty Animal Research
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    • 제34권4호
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    • pp.216-222
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    • 2018
  • The efficacy of standard therapeutic strategies for Helicobacter pylori (H. pylori) infection is decreasing over time due to the emergence of drug-resistant strains. As an alternative, the present study investigated the capacity of Lactobacilllus paracasei (L. paracasei) HP7, isolated from kimchi, to inhibit H. pylori growth. The effects of L. paracasei HP7 on H. pylori adhesion and H. pylori-induced inflammation were examined in AGS human gastric adenocarcinoma epithelial cells and a mouse model of H. pylori SS1 infection. L. paracasei HP7 reduced H. pylori adhesion to AGS cells and suppressed the inflammatory response in infected cells by downregulating interleukin-8. H. pylori colonization in the stomach of C57BL/6 mice was demonstrated by rapid urease test, and results showed significant decrease in mice post-treated with L. paracasei HP7. Additionally, L. paracasei HP7 decreased gastric inflammation and epithelial lesions in the stomach of H. pylori-infected mice. These results demonstrate that L. paracasei HP7 treatment can inhibit H. pylori growth and is thus a promising treatment for patients with gastric symptoms such as gastritis that are caused by H. pylori infection.

Baicalein의 BALB/c Mice에서의 접촉성 피부알레르기 예방효과 (Effects of Baicalein on Picryl Chloride-induced Contact Dermatitis in BALB/c Mice)

  • 김수현;김형진;정지윤
    • 한국식품영양과학회지
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    • 제38권2호
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    • pp.160-165
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    • 2009
  • 본 연구는 황금추출물인 baicalein의 피부알레르기 예방 효과를 알아본 연구로서 baicalein을 투여할 농도에 따라 0 mg/kg(대조군), 50 mg/kg(저용량군), 100 mg/kg(고용량군) 3개의 그룹으로 나누고, ear swelling을 측정하는 1군과 IgE 농도와 histamine 농도의 측정 및 조직병리학적 관찰을 위한 2군으로 나눠 실험을 하였다. Baicalein을 8일에 걸쳐 8회 경구투여를 실시한 후 접촉성 피부알레르기 유발 물질인 PCL을 귀에 감작시켜 알레르기를 유발시키고, 1군에서 ear swelling 변화를 확인하고 2군에서는 1군의 ear swelling의 결과에서 귀의 두께가 최고치를 이루는 시간대에 부검을 실시하여 적출한 조직으로는 HE염색과 TB염색을 실시하여 병변의 유무와 비만세포를 확인하였다. 채취한 혈청으로는 IgE 농도와 histamine 농도를 측정하였다. Ear swelling의 경우 0 mg/kg 농도의 baicalein을 투여한 대조군에 비해 50 mg/kg, 100 mg/kg 농도의 baicalein을 투여한 저용량군과 고용량군 그룹의 귀의 두께 증가폭이 낮게 나타났고, 저용량군보다 고용량군에서 그 증가폭이 낮게 나타났다. IgE 농도와 histamine 농도 측정결과에서도 0 mg/kg 농도의 baicalein을 투여한 대조군에 비해 50 mg/kg, 100 mg/kg 농도의 baicalein을 투여한 저용량군과 고용량군에서 낮은 수치가 나왔다. 조직병리학적 검사결과에서는 육안적으로 모든 그룹의 간과 신장에서는 별다른 병변은 관찰되지 않았고, HE염색을 한 귀 조직에서는 대조군에서 고용량군에 비해 귀의 두께가 두껍게 관찰되었고, TB염색을 한 귀 조직에서는 대조군에 비해 고용량군에서 적은 수의 비만세포들이 관찰되었다. 즉, ear swelling, IgE 농도, histamine 농도 및 조직병리학적 결과를 종합해봤을 때 황금추출물인 baicalein이 접촉성 피부알레르기 예방효과가 있다고 판단되어지고, 향후 baicalein은 항알레르기 치료제로 사용되는 생약제의 주요 성분으로 유용할 것이라 사료된다.

Pneumonia caused by Neisseria animaloris in a cat

  • Soo-Yeon Jeong;Doo-Sung Cheon;Chul Park
    • 대한수의학회지
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    • 제64권1호
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    • pp.1.1-1.4
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    • 2024
  • Neisseria (N.) animaloris is a common flora in animals, but its pathogenicity is rarely reported. In this case report, N. animaloris was isolated from a hospitalized cat with pneumonia. The cat was discharged after testing and treatment with appropriate antibiotics. This paper reports the first case of N. animaloris pneumonia in Korea.

A 16S rDNA polymerase chain reaction assay to detect Mycoplasma pulmonis in rats model

  • Hong, Sunhwa;Lee, Hyun-A;Choi, Yeon-Shik;Chung, Yungho;Kim, Okjin
    • 한국동물위생학회지
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    • 제38권2호
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    • pp.101-106
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    • 2015
  • Murine mycoplasmosis, caused by Mycoplasma (M.) pulmonis, is a prominent disease in rodent animals. The aim of this study was to develop a sensitive and specific PCR assay to detect M. pulmonis in animals and to assess the suitability of this assay for the detection of mycoplasmal infection in rats experimentally infected with M. pulmonis. A new PCR assay using the M. pulmonis-specific primer pairs MPul-F and MPul-R was developed. The primers and probe for the assay were designed from regions in the 16S rRNA gene that are unique to M. pulmonis. The novel PCR assay was very specific and sensitive for M. pulmonis, detecting the equivalent of 5 pg of target template DNA. It detected only M. pulmonis and no other Mycoplasma species or other bacterial species. The newly developed PCR assay also effectively detected M. pulmonis infection in rats. These results suggest that this PCR assay using M. pulmonis-specific primer pairs of MPul-F and MPul-R will be useful and effective for monitoring M. pulmonis infection in animals.