Korean Journal of Veterinary Service (한국동물위생학회지)

The Korean Society of Veterinary Service (한국동물위생학회)
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Comparison of the pathogenicity among Cronobacter species in a neonatal mouse model

  • Hong, Sun-Hwa (Center for Animal Resource Development, Wonkwang University) ;
  • Chung, Yung-Ho (Department of Companion Animal and Animal Resources Science, Joongbu University) ;
  • Park, Sang-Ho (Korea DNA Valley Co. Ltd.) ;
  • Kim, Ok-Jin (Center for Animal Resource Development, Wonkwang University)
  • Received : 2013.01.26
  • Accepted : 2013.03.13
  • Published : 2013.06.30
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Abstract

Neonatal infection caused by Cronobacter species can result in serious illnesses such as bacteremia, septicemia, meningitis, and death in at-risk infants who are orally fed contaminated reconstituted powdered infant formulas. The objective of this study was to compare the virulence among three Cronobacter species strains by using an animal model for human neonatal Cronobacter species infections. We acquired timed-pregnant ICR mice and all owed them to give birth naturally. On postnatal day 3, each pup was administered orally a total dose of $1{\times}10^7$ CFU Cronobacter species strain 3439, CDC 1123-79, and 3231. Mice were observed twice daily for morbidity and mortality. At postnatal day 10, the remaining pups were euthanized, and brain, liver, and cecum were excised and analyzed for the presence of Cronobacter species. Cronobacter species were isolated from cecum and other tissues in inoculated mice. In the tissues of Cronobacter species infected mice, meningitis and gliosis were detected in the brain. In this study, we identified the virulence among Cronobacter species strains by using a neonatal mice model which was a very effective animal model for human neonatal Cronobacter species infections.

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References

  1. Bar-Oz B, Preminger A, Peleg O, Block C, Arad I. 2001. Enterobacter sakazakii infection in the newborn. Acta Paediatr 90: 356-358. https://doi.org/10.1080/080352501300067857
  2. Beuchat LR, Kim H, Gurtler JB, Lin LC, Ryu JH. Richards GM. 2009. Cronobacter sakazakii in foods and factors affecting its survival, growth, and inactivation. Int J Food Microbiol 136: 204-213. https://doi.org/10.1016/j.ijfoodmicro.2009.02.029
  3. Drudy D, Mullane NR, Quinn T, Wall PG, Fanning S. 2006. Enterobacter sakazakii: an emerging pathogen in powdered infant formula. Clin Infect Dis 42: 996-1002. https://doi.org/10.1086/501019
  4. Farmer JJ III, Hickmann AM, Brenner DJ. 1980. Enterobacter sakazakii: a new species of 'Enterobacteriaceae' isolated from clinical specimens. Int J Syst Evol Microbiol 30: 569-584.
  5. Ghiara P, del Giudice G, Rappuoli R. 1999. Mouse model of Helicobacter pylori infection. pp. 265-272. In: Zak O, Sande, MA(ed.). Handbook of animal models and infection: Experimental Models in Antimicrobial Chemotherapy. Academic Press, San Diego.
  6. Iversen C, Forsythe SJ. 2003. Risk profile of Enterobacter sakazakii, an emergent pathogen associated with infant milk formula. Trends Food Sci Technol 14: 443-454. https://doi.org/10.1016/S0924-2244(03)00155-9
  7. Iversen C, Mullane N, McCardel B, Tal BD, Lehner A, Fannin S, Stephan R, Joosten H. 2008. Cronobacter gen. nov., a new genus to accommodate the biogroups of Enterobacter sakazakii, and proposal of Cronobacter sakazakii gen. nov., comb. nov., Cronobacter malonaticus sp. nov., Cronobacter turicensis sp. nov., Cronobacter muytjensii sp. nov., Cronobacter dublinensis sp. nov., Cronobacter genomospecies 1, and of three subspecies, Cronobacter dublinensis subsp. dublinensis subsp. nov., Cronobacter dublinensis subsp. lausannensis subsp. nov. and Cronobacter dublinensis subsp. lactaridi subsp. nov. Int J Syst Evol Microbiol 58: 1442-1447. https://doi.org/10.1099/ijs.0.65577-0
  8. Iversen C, Waddington M, On SL, Forsythe S. 2004. Identification and phylogeny of Enterobacter sakazakii relative to Enterobacter and Citrobacter species. J Clin Microbiol 42: 5368-5370. https://doi.org/10.1128/JCM.42.11.5368-5370.2004
  9. Lai KK. 2001. Enterobacter sakazakii infections among neonates, infants, children, and adults: case reports and a review of the literature. Medicine(Baltimore) 80: 113-122.
  10. Lee HA, Hong S, Chung Y, Kim O. 2011. Sensitive and specific identification by polymerase chain reaction of Eimeria tenella and Eimeria maxima, important protozoan pathogens in laboratory avian facilities. Lab Anim Res 27: 255-258. https://doi.org/10.5625/lar.2011.27.3.255
  11. Nazarowec-White M, Farber JM. 1997. Enterobacter sakazakii: a review. Int J Food Microbiol 34: 103-113. https://doi.org/10.1016/S0168-1605(96)01172-5
  12. Park JH, Lee YD, Ryu TW, Chang HI. 2010. Identification and classification of Cronobacter spp. isolated from powdered food in Korea. J Microbiol Biotechnol 20: 757-762. https://doi.org/10.4014/jmb.0912.12004
  13. Richardson AN, Lambert S, Smith MA. 2009. Neonatal mice as models for Cronobacter sakazakii infection in infants. J Food Prot 72: 2363-2367. https://doi.org/10.4315/0362-028X-72.11.2363
  14. Richardson AN, Pollak EA, Williams D, Agyekum AK, Smith MA. 2012. Susceptibility to Cronobacter sakazakii decreases with increasing age in neonatal CD-1 mice. J Food Prot 75: 884-888. https://doi.org/10.4315/0362-028X.JFP-11-139
  15. Urmenyi AMC, Franklin AW. 1961. Neonatal death from pigmented coliform infection. Lancet 1: 313-315.
  16. Willis-Owen SA, Flint J. 2006. The genetic basis of emotional behaviour in mice. Eur J Hum Genet 14: 721-728. https://doi.org/10.1038/sj.ejhg.5201569