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Optimal Growth Conditions for the Two Euryhaline Cyanobacterial Clones, Anabaena sp. CB-MAL21 and CB-MAL22 Isolated from Mankyeong Estuary, Korea

  • Kim, Young-Geel;Myung, Geum-Og;Yih, Won-Ho;Shin, Yoon-Keun
    • ALGAE
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    • v.19 no.2
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    • pp.145-148
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    • 2004
  • As a result of the 2-year monthly monitoring of the phytoplankton community at 3 stations in Mankyeong Estuary, Korea, we learned that cyan bacterial species of the genus Anabaena occurred at most sampling points with huge salinity differences (0.1-32.5 psu). We isolated several clones of Anabaena spp. from the monitoring stations, and screen out two euryhaline and nitrogen-fixing Anabaena clones, CB-MAL21 and CB-MAL22. The two clones were grown under various environmental gradients such as temperature (20, 30, 35 and 40$^{\circ}C$), salinity (0, 2, 5, 15 and 30psu), and $PO_4^{3-}$-P concentration (0, 1.6, 8.0, 40 and 200 ${\mu}M$M). Growth of CB-MAL21 and CB-MAL22 was measured by daily monitoring of chlorophyll fluorescence from each experimental culture for more than three serial transfers. Both the two experimental clones did not grow at 0psu. Maximal growth rates of the two clones were markedly reduced at lower $PO_4^{3-}$-P concentrations showing negligible growth at 0 and 1.6 ${\mu}M$M. However, growth of CB-MAL21 was not affected by low $NO_3^--$ concentration in culture media, showing the nitrogen-fixing ability. Maximum biomass yields of the two clones decreased dramatically at 35 and 40$^{\circ}C$. Optimal growth conditions for the two experimental clones were determined to be 20-30$^{\circ}C$, 40 ${\mu}M$M $PO_4^{3-}$-P, and wide salinity range from 5.0 to over 30psu. Best growth of CB-MAL21 was shown at (20$^{\circ}C$-15psu), which is less saline and cooler condition than those (i.e., 30$^{\circ}C$-30psu) for the best growth of CB-MAL22. The euryhaline and nitrogen-fixing CB-MAL21 strain thus can be a candidate laboratory culture for the future cyan bacterial marine biotechnology in temperate coastal waters.

Present Status and Development Strategies of Maglev in Korea (자기부상열차(磁氣浮上列車) 기술체계(技術體系)와 개발전략(開發戰略))

  • Yoo, Mun-Hwan;Kim, In-Kun
    • Proceedings of the KIEE Conference
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    • 1991.07a
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    • pp.102-105
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    • 1991
  • In recognition of the transportation problems of the present and to prepare for the ever increasing demands of the future, government decided to develop the magnetically levitated train domestically and started R&D program office in Korea Institute of Machinery and Metals(KIMM). This office since has established three step by step goals : first to develop a 40 passenger exhibition vehicle for Daejon EXPO'93, second to develop the low to mid-speed maglev system for urban public transportation by 1997 and finally the high speed inter-city maglev train by year 2001. The first two maglev systems will use attractive levitation-LIM driven technologies and these technologies are the ones currently being developed by this office and others. The maglev train system is a product of wide range of technologies from electro-technologies to civil engineering technologies. Some of the technologies are currently available but more have to be developed in the near future and these technologies are owned by or to be developed by various institutions within the science & technology community. The level of the technologies available at the present time are still very rudimentary and their basis are very narrow. Recently we have made a few successes in terms of levitation and propulsion but they are only with small scale modules and results are very qualitative at best. A great deal of development work has yet to be done to refine the technologies and to gain confidence. Full scale levitation/propulsion modules will be tested on the curved guideway within 6 months by this office and another institution. This paper reviews the current status of the maglev technologies in Korea and discuss the development strategies. The Korean maglev program is very ambitious and the schedule is even more so. A steady financial support and strong system engineering and integration are essential to the success of this program.

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A Study on Revitalization of Rural Traditional Theme Villages -Focused on Wanju Digital Sannaegol- (농촌전통테마마을의 활성화 방안에 관한 연구 -완주 디지털 산내골을 중심으로-)

  • Kim, Myoung-Ryong
    • Journal of Agricultural Extension & Community Development
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    • v.15 no.3
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    • pp.499-531
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    • 2008
  • Rural Traditional Theme Villages are one of the village-based businesses, which obtains excellent results. However, it also has brought negative results such as splits in locals due to income disparity, and ill-advised development which does not consider surrounding potential resources, etc. So, this study analyzes several problems of Rural Traditional Theme Villages, and proposes how Rural Traditional Theme Villages can be revitalized by benchmarking other successful cases. First, it is important to pursue differentiation of operating programs for Rural Traditional Theme Villages. Second, it is possible to give weight to elements which show efficiently rural atmosphere to enhance images of Rural Traditional Theme Villages. Third, expanding budget support and securing a new route for budget are definitely needed to cultivate self-growth capability of villages. Fourth, it is necessary to apply experts and facilities of local-based universities, cooperate with relevant organizations, and nurture local leaders by various means such as study in/visits to advanced regions at home and abroad and experts training. Localgovernment should be in charge of nurturing talented people. Fifth, it is necessary to reinvigorate rural economy by networking each Rural Traditional Theme Village, providing a wide range of information on diversified villages to people in cities, and promoting consumption. Moreover, securing immovable customers is important through networking city-rural exchange with city consumers and other organizations, and sisterhood relationship, membership businesses and events. As mentioned above, it is expected that Rural Traditional Theme Villages will be able to be developed continuously as a model for rural revitalization, which is stagnant, by expanding city-rural interchange.

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A study on the multilateralism in aircraft and air liners nationality and its implication with respect to the Article 7 of the Chicago Convention (항공기(航空機) 및 항공사(航空社)의 국적(國籍) 다원화(多元化)와 시카고 조약(條約) 제7조의 해석(解釋) 문제(問題))

  • Shin, Hong-Kyun
    • The Korean Journal of Air & Space Law and Policy
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    • v.7
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    • pp.151-175
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    • 1995
  • In recent years, questions have arisen under several forms with respect to the need for adapting present legal order established under the Chicago Convention and relevant customary rules into newly developed environment surrounding the international air transport industry. Major feature of such trends included in opinions for modification of the present legal order might be defined as more liberalistic approach to this industry. In this respect, many scholars and lawyers in this field agree with a view that a theoretical tie between an aircraft/air liners and a register - State lies in political and strategical concern of the State so that each aircraft/air liners has been attributed a single nationality. In the context of such concern, each aircraft/air liners has been related with each register-State in the form of "genuine connection". However, present and near future development of air transport industry and its world - wide market requires some modification of such single nationality regime. Taking into account such circumstances, States as creator of present legal order are in the process of establishing new legal order where air liners with multi - nationality are capable of satisfying to such needs. As adopting a series of liberalization package for air transport industry in european continent, European Union adopts a concept of "community air carrier", by which an air space of each member State is open to each other, especially through the grant of cabotage right. A serious concern may arise in such grant because the Article 7 of the Chicago Convention prohibits such grant on an exclusive basis. While many theoretical opinions have been put forward concerning the interpretation of that article, a case of European Union shall be a good test of the range of its application. It is anticipated that future development around this issue shaH furnish us a major feature of the liberalization of international air transportation and an adaptation process of present legal order.

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Coexistence of plant species under harsh environmental conditions: an evaluation of niche differentiation and stochasticity along salt marsh creeks

  • Kim, Daehyun;Ohr, Sewon
    • Journal of Ecology and Environment
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    • v.44 no.3
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    • pp.162-177
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    • 2020
  • Background: Ecologists have achieved much progress in the study of mechanisms that maintain species coexistence and diversity. In this paper, we reviewed a wide range of past research related to these topics, focusing on five theoretical bodies: (1) coexistence by niche differentiation, (2) coexistence without niche differentiation, (3) coexistence along environmental stress gradients, (4) coexistence under non-equilibrium versus equilibrium conditions, and (5) modern perspectives. Results: From the review, we identified that there are few models that can be generally and confidently applicable to different ecological systems. This problem arises mainly because most theories have not been substantiated by enough empirical research based on field data to test various coexistence hypotheses at different spatial scales. We also found that little is still known about the mechanisms of species coexistence under harsh environmental conditions. This is because most previous models treat disturbance as a key factor shaping community structure, but they do not explicitly deal with stressful systems with non-lethal conditions. We evaluated the mainstream ideas of niche differentiation and stochasticity for the coexistence of plant species across salt marsh creeks in southwestern Denmark. The results showed that diversity indices, such as Shannon-Wiener diversity, richness, and evenness, decreased with increasing surface elevation and increased with increasing niche overlap and niche breadth. The two niche parameters linearly decreased with increasing elevation. These findings imply a substantial influence of an equalizing mechanism that reduces differences in relative fitness among species in the highly stressful environments of the marsh. We propose that species evenness increases under very harsh conditions if the associated stress is not lethal. Finally, we present a conceptual model of patterns related to the level of environmental stress and niche characteristics along a microhabitat gradient (i.e., surface elevation). Conclusions: The ecology of stressful systems with non-lethal conditions will be increasingly important as ongoing global-scale climate change extends the period of chronic stresses that are not necessarily fatal to inhabiting plants. We recommend that more ecologists continue this line of research.

A Study on the Design Characteristic of Outdoor Spaces in the Apartment Complex - Focused on the Good Design Awarded Apartment Complex in the Past Decade - (국내 아파트 단지 외부공간의 디자인 특성 분석 - 최근 10년간 굿 디자인(Good Design) 수상 아파트 단지를 대상으로 -)

  • Jung, Hyun-Won;Oh, Jung-Ah
    • Korean Institute of Interior Design Journal
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    • v.24 no.1
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    • pp.160-168
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    • 2015
  • The subject of this research is to analyze the design characteristics of outdoor spaces in Good Design awarded apartment complexes in the past decade. The results are as follows. First, the overall outdoor space design theme, one of the conceptual elements, is focused on both 'nature' and 'story of various subject' that are similar to each other. Second, the case study object with the visual openness, which is secured between apartment blocks, is shown frequently in case box-shaped apartment blocks are arranged in a row. However, in case of Y-shaped block arrangement, the visual openness secured within an apartment complex is considered more important. Third, according to the result of circulation/ inner path element, the most frequently appeared inner path form is a grid pattern with straight path emphasis. While apartment complexes with the parking lot on the ground are only 3 out of 18 cases, it with circulation path for vehicle on the ground level are over 61%. Fourthly, the design characteristic of social space of physical outdoor design appears to be 'central link facility type'. In this case, the central community facilities are more likely to be combined with various functions, such as green, water, and social connection. Fifthly, in case of natural space, most of the apartment cases are shown over 45% green area ratio with wide green and water space provision. Especially, the differentiation is to provide the space where can stimulate a resident's participation.

A remote long-term and high-frequency wind measurement system: design, comparison and field testing

  • Zhao, Ning;Huang, Guoqing;Liu, Ruili;Peng, Liuliu
    • Wind and Structures
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    • v.31 no.1
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    • pp.21-29
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    • 2020
  • The wind field measurement of severe winds such as hurricanes (or typhoons), thunderstorm downbursts and other gales is important issue in wind engineering community, both for the construction and health monitoring of the wind-sensitive structures. Although several wireless data transmission systems have been available for the wind field measurement, most of them are not specially designed for the wind data measurement in structural wind engineering. Therefore, the field collection is still dominant in the field of structural wind engineering at present, especially for the measurement of the long-term and high-frequency wind speed data. In this study, for remote wind field measurement, a novel wireless long-term and high-frequency wind data acquisition system with the functions such as remote control and data compression is developed. The system structure and the collector are firstly presented. Subsequently, main functions of the collector are introduced. Also novel functions of the system and the comparison with existing systems are presented. Furthermore, the performance of this system is evaluated. In addition to as the wireless transmission for wind data and hardware integration for the collector, the developed system possesses a few novel features, such as the modification of wind data collection parameters by the remote control, the remarkable data compression before the data wireless transmission and monitoring the data collection by the cell phone application. It can be expected that this system would have wide applications in wind, meteorological and other communities.

Morphological Variation and Recovery Mechanism of Residual Crude Oil by Biosurfactant from Indigenous Bacteria: Macro- and Pore-Scale Experimental Investigations

  • Song, Zhi-Yong;Han, Hong-Yan;Zhu, Wei-Yao
    • Journal of Microbiology and Biotechnology
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    • v.25 no.6
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    • pp.918-929
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    • 2015
  • Microbial enhanced oil recovery (MEOR) is being used more widely, and the biological contributions involved in MEOR need to be identified and quantified for the improvement of field applications. Owing to the excellent interfacial activity and the wide distribution of producing strains in oil reservoirs, lipopeptides have proved to be an essential part of the complex mechanisms in MEOR. In this study, crude lipopeptides were produced by a strain isolated from an indigenous community in an oil reservoir. It was found that crude lipopeptides can effectively reduce the IFT (interfacial tension) to 10-1~10-2 mN/m under high salinity without forming stable emulsions, and the wettability of natural sandstone can be enhanced (Amott index, from 0.36 to 0.48). The results of core flooding experiments indicate that an additional 5.2% of original oil in place can be recovered with a 9.5% reduction of injection pressure. After the shut-in period, the wettability of the core, the reduction of injection pressure, and the oil recovery can be improved to 0.63, 16.2% and 9.6%, respectively. In the microscopic flooding experiments, the crude oil in membrane, cluster, and throat states contribute nearly 90% in total of the additional oil recovery, and the recovery of membranestate oil was significantly enhanced by 93.3% after shut in. Based on the results in macro and pore scale, the IFT reduction and the wettability alteration are considered primary contributors to oil recovery, while the latter was more dominant after one shut-in period.

$Mycoplasma$ $pneumoniae$ pneumonia in children

  • Youn, You-Sook;Lee, Kyung-Yil
    • Clinical and Experimental Pediatrics
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    • v.55 no.2
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    • pp.42-47
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    • 2012
  • $Mycoplasma$ $pneumoniae$ (MP), the smallest self-replicating biological system, is a common cause of upper and lower respiratory tract infections, leading to a wide range of pulmonary and extra-pulmonary manifestations. MP pneumonia has been reported in 10 to 40% of cases of community-acquired pneumonia and shows an even higher proportion during epidemics. MP infection is endemic in larger communities of the world with cyclic epidemics every 3 to 7 years. In Korea, 3 to 4-year cycles have been observed from the mid-1980s to present. Although a variety of serologic assays and polymerase chain reaction (PCR) techniques are available for the diagnosis of MP infections, early diagnosis of MP pneumonia is limited by the lack of immunoglobulin (Ig) M antibodies and variable PCR results in the early stages of the infection. Thus, short-term paired IgM serologic tests may be mandatory for an early and definitive diagnosis. MP infection is usually a mild and self-limiting disease without specific treatment, and if needed, macrolides are generally used as a first-choice drug for children. Recently, macrolide-resistant MP strains have been reported worldwide. However, there are few reports of apparent treatment failure, such as progression of pneumonia to acute respiratory distress syndrome despite macrolide treatment. The immunopathogenesis of MP pneumonia is believed to be a hyperimmune reaction of the host to the insults from MP infection, including cytokine overproduction and immune cell activation (T cells). In this context, immunomodulatory treatment (corticosteroids or/and intravenous Ig), in addition to antibiotic treatment, might be considered for patients with severe infection.

A Study on Problems and Attentive of Incoterms$^{(R)}$ 2010 Rules (Incoterms$^{(R)}$ 2010 Rules의 문제점과 대안)

  • Oh, Se-Chang
    • THE INTERNATIONAL COMMERCE & LAW REVIEW
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    • v.51
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    • pp.3-54
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    • 2011
  • The important and main purpose of revision of Incoterms rules are to adapt it to contemporary commercial practice in world trade, and to contribute to great service to foreign trade. Incoterms$^{(R)}$ 2010 revision basically focuses on trade terms to be used for any mode or modes of transport at compared Incoterms 2000 gouping in four basically different categories, and presents trade terms to be used for traditionally seaborn trade. Therefore the former is for trading in manufactured goods involved in containerization, the latter is for trading in community. This study focuses on a motive of revisions and main features of the Incoterms$^{(R)}$ 2010 rules, an outline, the problems and alternative of them. In conclusion, I would like present as follows; (1) Although they only concerned the models of delivery and critical point, they only say a few of the many factors of a sale contract, that is to say for the devision of fuctions, costs and risks between the contracting parties as supplement law, but the guestion of the legal position of Incoterms rules is various in different countries and scholars. in spite of that, it must focus on the practical application and the wide acceptance of Incoterms rules in world trade. (2) Although they present more simple and clear, unfricative, than Incoterms 2000 rules, in order to help users, the need to unify consistently and fully explanate in contents and expression. (3) Introduction and guidance note of Incoterms$^{(R)}$ 2010 rules basically focuses on the understanding of a motive of revisions as compared Incoterms 2000 rules. But it is well advised to do this on the assumption of understanding the various basic meaning of terms. (4) finally, trade concerned regulations take account of the reflection for the application to domestic and international trade according to formally reconization of availability for both trade.

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