• Journal of Microbiology
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• v.42 no.1
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• pp.9-13
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• 2004

Culture-independent approaches, based on 16S rDNA sequences, are extensively used in modern microbial ecology. Sequencing of the clone library generated from environmental DNA has advantages over fingerprint-based methods, such as denaturing gradient gel electrophoresis, as it provides precise identification and quantification of the phylotypes present in samples. However, to date, no method exists for comparing multiple bacterial community structures using clone library sequences. In this study, an automated method to achieve this has been developed, by applying pair wise alignment, hierarchical clustering and principle component analysis. The method has been demonstrated to be successful in comparing samples from various environments. The program, named CommCluster, was written in JAVA, and is now freely available, at http://chunlab.snu.ac.kr/commcluster/.

• Journal of the Korean Institute of Landscape Architecture
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• v.28 no.6
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• pp.1-15
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• 2001

The purpose of this study is to figure out the structural characteristics of urban plant community and suggest restoration model of Quercus mongolica in the case of Korean national Capital Region. The investigation areas were selected from urban area of Mt. Nam at Chung-Gu, suburban areas of Mt. Bong at Eunpyoung-GU, Mt. Sungju at Buchon City and non-urban areas of Mt. Suri at Kunpu City and Mt. Chonma t namyangju-City. After the main study field had been classified into the evaluation of the ecological characteristics and the modeling of the vegetation. We analyzed to evaluate the ecological characteristics of the forest structure -- successional stage, naturalness, multi-layer structure of the forest and species diversity, and the plant community structures. We have proposed vegetation restoration model based on the selection of proper plants, the number of individuals, diameter short area of breast height, the shortest distance between plants in non-urban area. As for successional stage, It was judged that the ecological succession may not be followed like the present stage of the surveyed areas in urban, suburban and non-ruban areas. As for the retention of naturalness and multi-layer structures of vegetation, In Quercus mongolica community, Robinia pseudo-acacia and Ailanthus altissima occurred in each layers at Mt. Nam, Mt. Bong and Mt. Sungju, and Eupatorium rugosum occurred in herbaceous layer at Mt. Nam. Consequently, the ecological restoration plan following the structure of the vegetation in Mt. Chonma seemed to be advisable in Q. mongolica community, there were less number of species and individuals in urban areas than those of non-urban areas. Planting of trees following the simulated native plant community of non-urban areas seemed to be required to promote the plants in urban areas. Considering the number of individuals up to three layers in each 400$m^2$ area, it was composed of twenty nine in canopy layer, forth nine in understory layer, 367 in shrub layer and 33.7% herbaceous ground cover in the Q.mongolica community. The suggested restoration model in this study is nan applicable model for the introduction in the cities, and this study shows that continuous experiments and field investigation on this model should be performed in the future.

• Journal of Korean Society of Environmental Engineers
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• v.32 no.4
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• pp.369-378
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• 2010

Microorganisms are key-role player for stabilization of landfill sites. In order to evaluate the availability of T-RFLP(Terminal Restriction Fragment Length Polymorphism) for monitoring microbial community variations during stabilization of landfill sites, the phylogenic diversity of microbial community in the leachate from 4 different full-scale landfills was characterized by T-RFLP based on bacterial 16S rDNA. Main population of microbial community analyzed by T-RFLP was significantly similar with that of microbial community analyzed by clone library analysis. The results of T-RFLP analysis for main population of microbial community in the leachate from landfills with different landfill structures, waste types and landfill ages showed apparently different microbial diversity and structures. Therefore, long-term monitoring of microbial community in leachate from landfill sites by using T-RFLP is expected to be available for evaluation of landfill stability.

• The Plant Pathology Journal
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• v.37 no.6
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• pp.521-532
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• 2021

Knowledge and better understanding of functions of the microbial community are pivotal for crop management. This study was conducted to study bacterial structures including Acidovorax species community structures and diversity from the watermelon cultivated soils in different regions of South Korea. In this study, soil samples were collected from watermelon cultivation areas from various places of South Korea and microbiome analysis was performed to analyze bacterial communities including Acidovorax species community. Next generation sequencing (NGS) was performed by extracting genomic DNA from 92 soil samples from 8 different provinces using a fast genomic DNA extraction kit. NGS data analysis results revealed that, total, 39,367 operational taxonomic unit (OTU), were obtained. NGS data results revealed that, most dominant phylum in all the soil samples was Proteobacteria (37.3%). In addition, most abundant genus was Acidobacterium (1.8%) in all the samples. In order to analyze species diversity among the collected soil samples, OTUs, community diversity, and Shannon index were measured. Shannon (9.297) and inverse Simpson (0.996) were found to have the highest diversity scores in the greenhouse soil sample of Gyeonggi-do province (GG4). Results from NGS sequencing suggest that, most of the soil samples consists of similar trend of bacterial community and diversity. Environmental factors play a key role in shaping the bacterial community and diversity. In order to address this statement, further correlation analysis between soil physical and chemical parameters with dominant bacterial community will be carried out to observe their interactions.

• Journal of Agricultural Extension & Community Development
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• v.8 no.1
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• pp.85-92
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• 2001

As the technology, global economy, and social structures and patterns have rapidly been changing since the 20th century, the forward-thinking leaders in the world begin to realize that they must embrace a new paradigm in management and organizational theories. The new idea of management is focused on equality rather than hierarchical structure, and more responsive to the external environment, more flexible, and better prepared to give customers what they want. From this new paradigm revolution, the nonprofit organizations like youth development organization are trying to approach and implement the new theories and models. This study attempted to introduce the historical trend and a new paradigm of youth development organizational structures and programs in the USA that may help Korea to improve youth organizations and programs. The youth development sector should create healthy, thriving organizations and structures that cultivate staff, programs, partnership between youth and adults, and community.

• Journal of Life Science
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• v.12 no.6
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• pp.649-653
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• 2002

The vegetation of Seoimal-lighthouse area was investigated from August, 2001 to July, 2002. In order to analyze the vegetation of this area, synthesis table, actual vegetation map were prepared. The main community structures of this area represent evergreen broad-leaf community(about 48%), which are very good condition(nearly last sere) to preserve and 11communities(Camellia japonica community, Castanopsis cuspidnta var. thunbergii community, Neolitsea sericea community, Quercus serrata community, Quercus variabilis community, Carpinus corenna community, Srtrax japonica community, Pinus thunbergii community, Quercus acutissima community, Zelkova serrata community, Cinnamimum japonicum community) were classified by vegetation type. And Daphne kiusiana, Asirum maculatum, Cymbidium goeringii, Chloranthus glaber, Ligularia taguetii should be worthwhile species to conserve in this area.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.50 no.3
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• pp.319-325
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• 2017

Marine algal flora and community structures were examined seasonally at three sites around the Shingori Nuclear Power Plant, Korea from February to November 2016. A total of 76 species were identified, including 9 green, 14 brown and 53 red algae. The greatest number of species occurred at Daesongri (51 species) followed by Sinamri (43 species) and at the discharge area (38 species) during the study period. Of the 76 seaweeds, 59 annuals and 17 warm tolerant species were recorded. Annual average biomass in dry weight varied from $168.02g/m^2$ at the discharge area to $222.53g/m^2$ at Sinamri. The coarsely branched form was the dominant functional form of seaweeds at the three sites. Seaweed community structures at the discharge site were distinguishable by decreasing species richness, biomass, species diversity index, richness index, and evenness index. The ratio of warm tolerance species and the dominance index values were remarkably higher at the discharge than those at the other sites.

• Asia pacific journal of information systems
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• v.33 no.2
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• pp.418-443
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• 2023

Many organizations facilitate a host of online knowledge sharing communities to assist internal knowledge sharing and operation. The permeable boundaries and voluntary structures of online communities allow individuals to span community boundaries and affect member resources and structures. Although much research has been done on members' knowledge contribution in online communities, relatively little is known about how a member's contribution to a community is shaped by the cross-level interactions of member's external boundary spanning and the community's environmental scanning or membership fluidity. Drawing from the theoretical lens of boundary spanning and the external view of online communities, we take a multi-level approach in the analysis of the activities of 1,992 members of 126 communities internal to a global company. We find that a member's external boundary spanning activity (e.g., external knowledge acquisition via reading posts) has a positive effect, though at a decreasing rate, on subsequent internal knowledge contribution (e.g., posting replies in the member's home community). This positive effect is stronger in communities that are more active in environmental scanning or have fluid membership and weaker in communities that are less active in environmental scanning or have stable membership.

• Korean Journal of Microbiology
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• v.38 no.3
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• pp.192-197
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• 2002

Fluorescent in situ hybridization (FISH) with rRNA-targeted oligonucleotide probes was used to compare the community structures of free-living and aggregated bacteria at thawing period in Lake Baikal. Targeted groups were Eubacteria, $\alpha$-, $\beta$-, $\gamma$- proteobacteria groups, Cytophaga-Flavobacterium group and Planctomycetales. Total bacterial numbers of free-living bacteria were ranged from $0.2{\times}10^6\cells{\cdot}ml^-1$ to $3.2{\times}10^6\cells{\cdot}ml^-1$, which were decreasing with depth, while the aggregated bacterial numbers were dramatically increasing from $0.4{\times}10^4 to 3.3{\times}10^4 \cells{\cdot}ml^-1$ with depth. The ratios of EUB probe binding cells to DAPI counts were ranged from 52.3 to 74.1% in free-living bacteria, and from 39.6 to 66.7% in the aggregated bacteria, respectively. Community structures of the aggregated bacteria were very different from each free-living bacteria at every depth. At 25 m depth, where the chlorophyll a concentration was highest, both structures were quite different from those of surface layers, rendering the fact that the community structures might be affected by phytoplankton. The vertical profile of community structure of aggregated bacteria is particular. The proportion of $\beta$-proteobacteria group was increasing with depth and it was 51.8% at 100 m, but the dominant group was $\gamma$-pro-teobacteria group at 250 m. Taken together, the biodiversity and succession of aggregated bacteria are quite different from free-living bacteria.

• Knowledge Management Research
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• v.12 no.2
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• pp.69-80
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• 2011

This paper reports on a structural view of a knowledge network comprised of clubs and organisationsexpressly concerned with cultural activities in a regional Australian city. Social network analysis showed an uneven distribution of power, influence, and prominence in the network. The network structure consisted of two modules of vertices clustered around particular categories of creative arts and these modules were linked most frequently by several organisations acting as communication hubs and boundary spanners. The implications of the findings include 'network weaving' for improving the network structure and developing a systemic approach for exploring the structures of social action that form community-based knowledge networks.