• Korean Journal of Organic Agriculture
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• v.19 no.spc
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• pp.195-198
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• 2011

To screen several promising red pepper cultivars, may be adaptable to organic cultivation conditions, seventy six F1 hybrids commercial and eight local purebred red pepper cultivars were tested. Comparing the infection rate of phytophthora blight between commercial (F1 hybrid) and local (Purebred) cultivars, average infection rate of commercial cultivars was 9.8% and that of local cultivars was 17.8%. But the infected fruits rate of anthracnose in field were similar as 3.3% in commercial (F1 hybrid) and 3.1% in local (Purebred) cultivars. In yield characteristics, average yield of commercial cultivars was 2.89 t $ha^{-1}$ and that of local cultivars was 2.22 t $ha^{-1}$. For organic pepper farmers it is more favourable to cultivation purebred cultivars because they can save to the same quality plant next year's crop. In this study among the local purebred cultivars, two cultivars are promising that their yield near to 3 t $ha^{-1}$ and have disease field resistance.

• Applied Biological Chemistry
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• v.14 no.3
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• pp.221-227
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• 1971

Potential grain yield of rice plant was calculated from potential grain yield of each leaf for two lines of IR 667 and two commercial varieties (Jinhung and Paldal). According to the percentage contribution of each leaf the pattern of potential grain yield of IR 667 lines could be grouped into the upper leaf-dependent type indicating fast senescence while that of commercial varieties could be grouped into the lower leaf-dependent type indicating shade resistance. The comparisons between potential grain yields and apparent grain yields indicate that the commercial varieties having a comparably unfavorable plant type thus remained much behind the potential yield. The significance of potential grain yield was discussed in relation to nutrio-physiology and breeding. Methods for potential yield determination were also discussed.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.23 no.6
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• pp.475-483
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• 1990

An assessment of Kuwait's commercial fish stocks: hamoor (Epinephelus tauvina), zobaidy (Pampus argenteus), nakroor (Pomadasys argenteus) and sheiry (Lethrinus nebulosus), was conducted using length-frequency data, mean growth and mortality estimates obtained during 1981$\~$1988. The length-cohort analysis indicated that increases in fishing effort would not lead to long-term gains in yield of the stocks at the current estimate of natural mortality rate (M). At high M which was assumed arbitrarily, some benefit in yield could be obtained, especially for hamoor and sheiry. At low M, the yield of all stocks decreased with increased fishing effort. Increases in fishing effort resulted in significant dec-line in spawning stock size for all the stocks. Yield-per-recruit analysis indicated that, un-der low M assumption, a higher yield can be obtained for zobaidy and nakroor by reducing fishing effort. At moderate M, decreases in fishing effort brought gains in yield per recruit of the stocks, but it was not substantial compared with the present level of M. At high M, most of the stocks reached the maximum yield-per-recruit. Overall, increased fishing effort either will not be associated with large long-term gains in yield or, in some stocks, might cause a decline from the present level.

• Journal of Marine Bioscience and Biotechnology
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• v.12 no.1
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• pp.50-58
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• 2020

Sphacelaria is a filamentous brown algal genus that can be epibiotic on macroalgae, marine plants, and sea turtles. Its important role in benthic ecosystems, exposure to different stressors (e.g., grazing), and use as a model organism make Sphacelaria ideal for assessing physiological responses of organisms to environmental inputs. Single-cell RNA sequencing is a powerful new probe for understanding environmental responses of organisms at the molecular (transcriptome) level, capable of delineating gene regulation in different cell types. In the case of plants, this technique requires protoplasts ("naked" plant cells). The existing protoplast isolation protocols for Sphacelaria use non-commercial enzymes and are low-yielding. This study is the first to report the production of protoplasts from Sphacelaria fusca (Hudson) S.F. Gray, using a combination of commercial enzymes, chelation, and osmolarity treatment. A simple combination of commercial enzymes (cellulase Onozuka RS, alginate lyase, and driselase) with chelation pretreatment and an increased osmolarity (2512 mOsm/L H₂O) gave a protoplast yield of 15.08 ± 5.31 × 10⁴ protoplasts/g fresh weight, with all the Sphacelaria cell types represented. Driselase had no crucial effect on the protoplast isolation. However, the increased osmolarity had a highly significant and positive effect on the protoplast isolation, and chelation pretreatment was essential for optimal protoplast yield. The protocol represents a significant step forward for studies on Sphacelaria by efficiently generating protoplasts suitable for cellular studies, including single-cell RNA sequencing and expression profiling.

• Korean Journal of Breeding Science
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• v.42 no.4
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• pp.344-350
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• 2010

Sweet pepper inbred lines (KNU1003, KNU1006, KNU1007, KNU1009, KNU1015, KNU1017 and KNU2006) developed at Kangwon National University (KNU) through conventional means, inbred lines (5AVS1, 5AVS2, 5AVS3, 5AVS5, 5AVS7 and 5AVS8) collected at Rural Development Administration (RDA) and inbred lines (SP12, SP27 and SP14) derived from anther culture were used as female parents and anther culture derived homozygous lines (SP9, SP10, SP14, SP24, SP25, SP27, SP30, SP32, SP34, SP38, SP43, SP45 and SP51) were used as male parents to produce $F_1$ hybrids. A total of 37 $F_1$ hybrids were evaluated for fruit yield and quality characters in summer season, 2007. Variation in fruit number, fruit weight, fruit yield per plant and fruit volume was observed among the $F_1$ hybrids. Superiority on yield over standard/commercial varieties were differed among $F_1$ hybrids. Hybrid $5AVS8{\times}SP45$ exhibited highest heterosis over Special (16.5%) and Fiesta (24.7%). Fruit quality characters (fruit length, fruit width, pericarp thickness, total soluble solid, fruit shape and fruit color) were varied among the $F_1$ hybrids. Fruit number, fruit weight and fruit volume per plant were correlated with fruit yield. Based on the standard heterosis expressed by the hybrids and quality characters evaluation, $KNU1017{\times}SP27$, $5AVS1{\times}SP43$, $5AVS5{\times}SP27$, $5AVS8{\times}SP45$, $SP12{\times}SP38$ and $SP27{\times}SP25$ hybrids were found to be superior over commercial cultivars and are selected. Inbred lines of these hybrid combinations can be used to produce $F_1$ hybrid seed for commercial production.

• Asian-Australasian Journal of Animal Sciences
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• v.11 no.3
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• pp.255-259
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• 1998

Sixteen Friesian Cows were allocated equally into 4 groups in a $4{\times}4$ Latin square design to evaluate the effects of utilizing bean by-products on the feed intake and milk production. Cows were fed on 4.5% of their body weight fresh alfalfa; the remainder of their requirements were covered by one of 4 experimental concentrate treatments: 1) commercial concentrate (CC), 2) 70% CC + 30% bean hulls (BH), 3) 70% CC + 30% Polished bean (PB) and 4) 70% CC+ 15% BH + 15% PB. Results showed that the inclusion of bean hulls at 30% improved (p<0.05) milk yield, 4% fat corrected milk yield, feed conversion ratio and fat percent of the milk over the other treatments. Feeding cows on a commercial concentrate plus 30% BH or 30% PB or 15% from each of BH and PB decreased the cost of feed per kg milk by 13.6, 2.5 and 1.3 %, respectively, lower than those fed on the commercial concentrate.

• Journal of Microbiology and Biotechnology
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• v.9 no.4
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• pp.514-517
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• 1999

Hydrolysis of EFB (empty fruit bunch) derived from oil palm was studied using crude enzyme from Aspergillus terreus IMI 282743 along with commercial enzymes from Trichoderma reesei and Aspergillus niger. Hydrolysis at $40^{\circ}C$ and $50^{\circ}C$ with $\alpha$-cellulose or EFB gave significantly lower yield when commercial enzymes of T. reesei and A. niger were used and the hydrolysis time extended beyond 10 h. After 24 h of hydrolysis at $40^{\circ}C$ and $50^{\circ}C$, the filter paper activity (Fpase) from A. terreus retained as much activity as A. niger and it was significantly higher than T. reesei. Glucose concentration of 0.25% and 0.5% caused significant inhibition in the crude enzyme, but in regards to the commercial enzymes it only showed a slight effect. Crude enzymes from A. terreus could produce the highest reducing sugars when compared to commercial enzymes from T. reesei or A. niger. Nevertheless, low yield of sugar was observed for EFB for all treatments.

• Korean Journal of Plant Resources
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• v.10 no.4
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• pp.375-381
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• 1997

This study was conducted to determine the effect of seedling and division nursery stock size on the growth and yield of Wasabia japonica cultivated with cold water from Soyang Dam. As seedling and division nursery stock size were enlarged, their growth and yield were higher. In the case of division nursery stock, the rhizome yield per 10a were 782kg for large one, 723kg form medium one and 567kg for small one, and commercial rates - the percentage of rhizomes that weigh over 40g - were 87% for large one,80% for medium one and 55% for small one. In the case of seedling, large one brought out yield of 575kg/10a while medium and small one brought out low yield of 408kg/10a, and 421kg/10a, respectively. And the commercial rates from seedling were 60% for large one. 13% for medium one and 20% for small one.

• Asian-Australasian Journal of Animal Sciences
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• v.22 no.5
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• pp.681-685
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• 2009

L-carnitine promotes mitochondrial ${\beta}$-oxidation of long chain fatty acids and their subsequent transport across the inner mitochondrial membrane. Although the role of L-carnitine in fatty acid metabolism has been extensively studied, its role in live performance and carcass responses of commercial broilers is less understood. The objective of this research was to determine if Lcarnitine fed at various levels in diets differing in CP and amino acids impacted on live performance and carcass characteristics of commercial broilers. Two floor pen experiments were conducted to assess the effect of dietary L-carnitine in grower diets. In Exp. 1, Ross${\times}$Hubbard Ultra Yield broilers were placed in 48 floor pens (12 birds/pen) and fed common diets to d 14. A two (0 or 50 ppm Lcarnitine) by three (173, 187, and 202 g/kg CP) factorial arrangement of treatments was employed from 15 to 35 d of age (8 replications/treatment). An interaction (p<0.05) in carcass yield indicated that increasing CP (187 g/kg) resulted in improved yield in the presence of L-carnitine. Increasing CP from 173 to 202 g/kg increased (p<0.05) BW gain and decreased (p<0.05) feed conversion and percentage abdominal fat. Feeding dietary L-carnitine increased back-half carcass yield which was attributable to an increase (p<0.05) in thigh, but not drumstick, yield relative to carcass. In Exp. 2, $Ross{\times}Ross$ 708 broilers were fed common diets until 29 d. From 30 to 42 d of age, birds were fed one of seven diets: i) 200 g/kg CP, 0 ppm L-carnitine; ii) 200 g/kg CP, 40 ppm L-carnitine; iii) 180 g/kg CP, 0 ppm L-carnitine; iv) 180 g/kg CP, 10 ppm L-carnitine; v) 180 g/kg CP, 20 ppm L-carnitine; vi) 180 g/kg CP, 30 ppm L-carnitine; and vii) 180 g/kg CP, 40 ppm L-carnitine (6 replications of 12 birds each). BW gain, feed conversion, mortality (30 to 42 d), and carcass traits (42 d) were measured on all birds by pen. There were no treatment differences (p<0.05). However, the addition of 40 ppm L-carnitine in the 200 g CP/kg diet increased (p = 0.06) thigh yields relative to BW in comparison to birds fed diets without L-carnitine, which was further confirmed via a contrast analysis (0 vs. 40 ppm L-carnitine in the 200 and 180 g CP/kg diets; p<0.05). These results indicated that dietary L-carnitine may heighten metabolism in dark meat of commercial broilers resulting in increased relative thigh tissue accretion without compromising breast accretion.

• 한국생물공학회:학술대회논문집
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• 2000.04a
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• pp.347-350
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• 2000

Enzymatic deinking of office-waste paper was studied using crude cellulase and papain-hydrolyzed cellulase from Trichoderma reesei Rut C-30 in small-scale and mid-scale. The results were compared with deinkings using commercial enzyme(Novozym 342) and conventional chemical methods. Maximum brightness and freeness were obtained at 3 units/g Oven Dry Paper(ODP) of CMCase activity using crude cellulase in mid-scale deinking experiments. The deinked pulp had higher physical strength and brightness, and lower freeness and yield than the pulp deinked in small scale. In small scale deinking, maximum brightness and freeness were obtained at 2 unit/g ODP. Deinking by papain-hydrolyzed cellulase showed similar results with one by Novozym 342. It was better in brightness and freeness, but showed lower physical strength and yield, than the conventional deinking by sodium hydroxide. The ratio of endo-1,4-glucanase and exo-1,4-glucanase components in papain hydrolyzed cellulase from T. reesei Rut C-30 was similar to that of commercial enzyme, Novozym 342, implicating a successful application as a deinking enzyme.