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중간(中間) 측정(測定) 주기(週期) (3-5년)를 이용(利用)한 인공림(人工林)의 흉고단면적(胸高斷面績) 추정(推定) 함수(函數)의 유도(誘導) (Derivation of Basal Area Projection Function for Forest Plantation Using Medium (3-5years) Measurement Cycles)

  • 이상현
    • 한국산림과학회지
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    • 제89권4호
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    • pp.463-469
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    • 2000
  • 이 연구는 다른 수종에 비해 상대적으로 빠른 생장을 보여 상업적으로 중요하게 여겨지는 뉴질랜드 사우스랜드 지역에 조림된 미송(美松) (Pseudotsuga menziesii Mirb. Franco)의 흉고단면적(胸高斷面績) 추정 함수 유도에 관한 것이다. 흉고단면적(胸高斷面績) 함수를 도출하기 위하여 중간 측정 주기의 영구 표본점 데이터가 사용되었고, 대수차분(代數差分) 방정식을 이용하여 흉고단면적(胸高斷面績) 함수식을 유도하였다. 모수(母數) 추정은 SAS의 비선형 루틴에 의하여 수행하였다. 다양한 생장 추정 함수 모델을 적용한 후 잔차를 분석하여 평균제곱오차가 가장 작고 잔차 패턴이 편의가 없는 생장식을 선발하여, 추가 독립변수를 적용하여 모델의 추정 정도를 분석하였다. 그 결과 여러 추정 생장 함수 중 지위지수(地位指數) 및 간벌주기를 독립변수로 포함한 Schumacher 다형곡선(多形曲線) 생장식이 가장 정밀한 추정을 나타내었다. 이 결과로 흉고단면적(胸高斷面績) 생장과 지위지수(地位指數)사이에는 양(+)의 상관관계(相關關係)가 있음을 알 수 있었다. 그리고 정의된 간벌주기는 흉고단면적(胸高斷面績)식의 정도(精度)를 높이는데 유용한 것으로 나타났다.

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Comparative Assessment of Diagnostic Performances of Two Commercial Rapid Diagnostic Test Kits for Detection of Plasmodium spp. in Ugandan Patients with Malaria

  • Bahk, Young Yil;Park, Seo Hye;Lee, Woojoo;Jin, Kyoung;Ahn, Seong Kyu;Na, Byoung-Kuk;Kim, Tong-Soo
    • Parasites, Hosts and Diseases
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    • 제56권5호
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    • pp.447-452
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    • 2018
  • Prompt diagnosis of malaria cases with rapid diagnostic tests (RDTs) has been widely adopted as an effective malaria diagnostic tool in many malaria endemic countries, primarily due to their easy operation, fast result output, and straightforward interpretation. However, there has been controversy about the diagnostic accuracy of RDTs. This study was conducted to evaluate the diagnostic performances of the 2 commercially available malaria RDT kits, RapiGEN Malaria Ag Pf/Pv (pLDH/pLDH) and Asan $EasyTest^{TM}$ Malaria Ag Pf/Pv (HRP-2/pLDH) for their abilities to detect Plasmodium species in blood samples collected from Ugandan patients with malaria. To evaluate the diagnostic performances of these 2 RDT kits, 229 blood samples were tested for malaria infection by microscopic examination and a species-specific nested polymerase chain reaction. The detection sensitivities for P. falciparum of Malaria Ag Pf/Pv (pLDH/pLDH) and Asan $EasyTest^{TM}$ Malaria Ag Pf/Pv (HRP-2/pLDH) were 87.83% and 89.57%, respectively. The specificities of the 2 RDTs were 100% for P. falciparum and mixed P. falciparum/P. vivax infections. These results suggest that the 2 RDT kits showed reasonable levels of diagnostic performances for detection of the malaria parasites from Ugandan patients. However, neither kit could effectively detect P. falciparum infections with low parasitaemia (<$500parasites/{\mu}l$).

Purification and Identification of Paenibacillus sp., Isolated from Diseased Larvae of Allomyrina dichotoma (Linnaeus, 1771) (Coleoptera: Scarabaeidae) in Insect Farms

  • Kang, Tae Hwa;Han, Sang Hoon;Weon, Hang Yeon;Lee, Young Bo;Kim, Namjung;Nam, Sung Hee;Park, Hae Chul
    • International Journal of Industrial Entomology and Biomaterials
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    • 제25권2호
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    • pp.195-203
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    • 2012
  • In reared populations of Allomyrina dichotoma, commercial insects, the skin of last instar larvae was changed softer with opaque white, and infested grubs eventually died. To clarify the cause of the symptom, we collected the larvae of A. dichotoma from five farms and examined their intestinal bacterial florae using pyrosequencing technique. From those results, a member of Paenibacillus was found only in the larvae showing the symptom of disease. Through PCR analysis using a Paenibacillus specific primer set, we obtained the partial 16S rRNA gene sequence and confirmed the microbe as Paenibacillus sp. For clear identification, a whole guts was extracted from each larva showing the sign of the disease and incubated at $70^{\circ}C$ for 15 min to isolate spore forming bacteria. After then, each content of guts was cultured on $MYPGP_{NAL}$ agar medium($12.5{\mu}g/ml$ of nalidixic acid) at $30^{\circ}C$. The 16S rRNA gene sequence analysis for the isolated bacteria showed that they were closely related to P. rigui(97.9% similarity), to P. chinjuensis(96.1% similarity), and to P. soli(95.3% similarity). Additional tests including API test and cellular fatty acid composition analysis were performed, but the strain couldn't be identified at species level, suggesting it may represent novel species of the genus Paenibacillus.

임목폐기물 파쇄칩을 활용한 녹화기술의 비탈면 적용성에 관한 연구 (A Study on the Use of Wood Waste for Slope Revegetation Techniques)

  • 고정현;허영진;이용구;김남춘
    • 한국환경복원기술학회지
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    • 제13권1호
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    • pp.47-56
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    • 2010
  • The main objectives of this comparative study were 1) to quantify the potential contribution of wood chips to revegetation on the cut slope in a constructed area, 2) to compare the floristic similarity of species composition whether existing of wood chips in the soil media or not. Wood chips were combined with soil media in the ratio of 30%. The thin layer (1 cm, 3 cm) soil media revegetation methods and the thick layer (10 cm) soil media revegetation method were constructed on the cut slopes by machineries respectively. Also, comparative experiments the existence of wood chips in the soil media were conducted to evaluate the effects of wood chips on revegetation works. The total size of quadrat was $300m^2$ ($100m^2{\times}3$). The results of monitoring for 3 years on cut slopes were as follows: 1) All the quadrat existed in the proper range for vegetation. 2) Species richness of quadrat including wood chips was 10% more than those of commercial soil medias. 3) The coverage rates of quadrat including wood chips were similar or superior to those of conventional methods. It can be concluded that the use of wood chips would be effective to promote establishment of diverse landscape and vegetation. However, it behooves to continue monitoring on succession of vegetation for ecological restoration. Finally, adequate wood chips deposit and gathering methods should be studied properly.

중엽형 한국잔디(Zoysia spp.)류의 형태적 특성과 RAPDs를 이용한 분류 (Morphological Characteristics of Medium-Leaf Type Zoysiagrasses (Zoysia spp.) and Their Classification Using RAPDs)

  • 최준수
    • 아시안잔디학회지
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    • 제24권2호
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    • pp.88-96
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    • 2010
  • 한국잔디류중 엽폭이 3~4 mm 정도를 보이는 중지류는 최근에 국내에서 가장 널리 이용되고 있는 잔디이다. 본 연구에서는 한국잔디류 중 중엽형 36 계통과 기본 5종의 근연관계를 확인해 보고자 RAPD 방법을 사용하였다. 형태적 특성조사는 엽폭, 잎각도, 최하위 잎의 잎집 길이, 엽모, 지상포복경의 색 등을 조사하였다. 여덟개의 프라이머를 이용하여 19개의 RAPD 마커를 확인하였다. 마커를 이용하여 유전적 유사도를 조사한 결과 0~0.736의 유전적 변이정도가 확인되었다. RAPD 마커를 이용하여 분류한 결과 3개 군으로 나뉘어 졌다. 제1군에는 들잔디와 함께 미국에서 육성된 'Zenith', 'Meyer'등이 포함되었다. 제2군에는 갯잔디, 왕잔디와 함께 '안양중지', '삼덕중지', '평동중지' 등이 포함되었다. 그리고 제3군은 제1군, 제2군과 비교해 유전적으로 거리가 멀게 나타났으며, 금잔디와 비단잔디가 포함되었다. 한국에서 주로 이용되고 있는 '안양중지', '삼덕중지', 그리고 '평동중지' 류는 갯잔디 및 왕잔디와 유전적 유사도가 높은 것으로 나타났다.

Body Weight Changes of Laboratory Animals during Transportation

  • Lee, Sung-Hak;Nam, Hyun-Sik;Kim, Jin-Sung;Cho, Hye-Jung;Jang, Yu-Mi;Lee, Eun-Jung;Choi, Eun-Sung;Jin, Dong-Il;Moon, Hong-Sik
    • Asian-Australasian Journal of Animal Sciences
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    • 제25권2호
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    • pp.286-290
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    • 2012
  • The majority of laboratory animals were transported from commercial breeders to a research facility by ground transportation. During the transportation, many biological functions and systems can be affected by stress. In this experiment, the change of body weight during the transportation was measured and the recovery periods from the transportation stress established based on the body weight changes. Total 676 laboratory animals which were aged between 3 to 9 wk old were studied. The transportation time taken from container packing to unpacking the container was approximately 24 h. The temperature of animal container was constantly maintained by air-conditioning and heating equipment. Rats were found to be more sensitive than mice. The body weight of rats was significantly decreased 3.71% (p<0.05) compared to the body weight of mice which decreased 0.9% There was no significant difference between the strains in the same species. When the changes of body weights were compared between delivery days, C57BL/6 mice showed the most variable changes compared to other species and strains. Consequently, C57BL/6 was more sensitive to stress than the other strains and the transportation process needs to be standardized to reduce between day variability. To establish the recovery periods from transportation stress, the body weight changes were measured during the acclimation period. Although the body weight of animals decreased during transportation, animals recovered their weight loss after the next day.

한·중 어업자원관리제도에 관한 비교연구 (A Comparative Study on Fisheries Resource Management System between Korea and China)

  • 차철표
    • 수산해양교육연구
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    • 제13권2호
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    • pp.146-167
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    • 2001
  • Korea and China are two opposite countries located aside Yellow Sea and co-utilize the East China Sea. The two countries are close together from geological point of view, however, the competitive development of resources was more emphasized than the cooperative development of resources between the two countries because the special policy relationship. Additionally, after the communist government of China was founded in 1949, the political conception between the two countries was quite different. Therefore the establishment of appropriate international fisheries co-operation was impossible, and the international management problems of fisheries resources in Yellow Sea and East China Sea were let alone. UN convention on the Law of the Sea came to force in 1994, Korea and China adopted the exclusive economic zone system in 1996. On the other hand, Fisheries Law in Korea was enacted in 1953 in order to management of fisheries resources, and also China was enacted fisheries law in 1986. The two countries control the fisheries effort through fisheries license system, meanwhile through prohibition fishing area, prohibition fishing period, limitation of net size, and limitation of body length to conserve and manage the fisheries resource. The serious management methods of resource management in the two countries are similar such as the creation of promptly decreased species and those species that have commercial value, discharge of fish seedling stock, settlement of artificial reef and clean of fishing ground. Therefore, the two countries should consider not only the improvement of formal law system, but also how to recover the fisheries resources in circumference water zone and how to improve the efficiency of fisheries resource management. Specially the settlement and management of artificial reef should be chosen in the area that have the highest benefit to two countries, and should establish the common management system of discharge of fish seedling stock. And the two countries should adopt the same criteria through technical management and limitation of net size, limitation of body length, and prohibition area of special fisheries to ensure the highest fisheries benefit of fisherman in the two countries and the highest efficiency of fisheries resource management.

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Influence of forestry host plants and rearing seasons on silk gland weight of tropical tasar silkworm, Antheraea mylitta (Lepidoptera: Saturniidae) under Doon valley conditions of Uttarakhand in India

  • Bhatia, Narendra Kumar;Yousuf, Mohd.;Tewary, Pankaj;Sharma, Satya Prakash
    • International Journal of Industrial Entomology and Biomaterials
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    • 제32권1호
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    • pp.1-11
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    • 2016
  • Tropical tasar silkworm, Antheraea mylitta is a commercial forest silkworm in India that produces tasar silk, but never experimented in Uttarakhand, a Himalayan state of India. A. mylitta express divergent phenotypic characters under different ecological conditions; so, we studied the effect of seven forest tree species in two rearing seasons on variability in silk gland weight of Daba (bivoltine) ecorace of A. mylitta at Forest Research Institute in Dehra Dun, Uttarakhand during 2012 and 2013. We used two-way completely randomized block factorial design and Post HOC Tukey's HSD test to analyse the collected data and there after carried out multiple regression analysis. Results indicated that silk gland weight differed significantly between rearing seasons (DF=1, F=2333.98, p <0.05), host plants (DF 6, F= 1516.25, p <0.05) and their interactions (DF=6, F=7.10, p <0.05). Higher silk gland weight was found in second rearing season than the first on all the host tree species. Terminalia alata fed A. mylitta larvae showed the highest silk gland weight of 8.03 and 9.47 g in first and second rearing seasons, followed by T. tomentosa (7.19 & 9.01g), T. arjuna (6.8 & 8.08 g) and L. speciosa (6.57 & 7.83 g) fed larvae, respectively. Post HOC Tukey's HSD test indicated that silk gland weight of L. speciosa and T. arjuna fed larvae in both the rearing seasons did not differ significantly. E.I. analysis also confirmed that T. alata, T. tomentosa, T. arjuna and L. speciosa are better in their order of merit than T. bellirica , T. chebula and L. tomentosa. Multiple regression analysis indicates that larval weight gain is a strong predictor (β=1.002, t=346.777, p = <0.05) for the silk gland weight of A. mylitta ; however, larval duration had significant negative regression weight (β=-0.270, t=-8.436, p = <0.05) on mean weight of silk gland.

전자코를 이용한 검정현미의 산지판별 (Application of Electronic Nose in Discrimination of the Habitat for Black Rice)

  • 조연수;한기영;김정호;김수정;노봉수
    • 한국식품과학회지
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    • 제34권1호
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    • pp.136-139
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    • 2002
  • 검정현미에 내포한 특이한 향기성분을 토대로 수입산 또는 국내산인지의 여부를 전자코로 분석하였다. 주성분 분석 결과 전반적으로 수입산과 국내산의 판별이 뚜렷하게 구별 가능하였다. 또한 인공신경망 분석 프로그램을 이용하여 학습시켜 원산지를 모르는 시료를 분석한 결과 국내산 검정현미가 수입산인지 국내산인지를 구별할 수 있었다. E-Nose 5000을 이용한 결과는 69종의 국내산 검정현미를 국내산으로 판별한 경우가 64종으로 92.75%이었고, 13종의 수입산 검정현미를 수입산으로 판별한 경우는 11종으로 92.31%의 높을 확률로 metal oxide sensor로만 구성된 전자코의 판별정도보다 더 정확하게 구별할 수 있었다.

북태평양 중부 해산어장에 있어서 저층 트롤의 어획실태 (Fishing investigation of trawl fisheries in sea mount of central northern Pacific)

  • 김영승;오택윤;조삼광;최석관;고정락;양원석
    • 수산해양기술연구
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    • 제41권3호
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    • pp.179-187
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    • 2005
  • This study was conducted in order to the experiment of bottom trawl fisheries in the sea mount of central northern pacific($30^{\circ}-40^{\circ}N$, $170^{\circ}-175^{\circ}E$), during the period of July 1 to August 25, 2004 by commercial trawl ship. Fishing experiment was carried out by bottom trawl, with the codend of 110mm mesh size. The number of 101 test fishing was done in the sea mount of central northern pacific during 56 days and the total catches were 198,593kg as 18 fish species, and CPUE/day and catches/day were 8.3 hours towing time and 3,546kg. Catch ratio according to each fishing ground was higher with the order of B, C, and E, and catches for B was 7,171.0kg during the towing time of 13.2 hours. Main target species were Pseudopentaceros richardsoni and Beryx splendens, there was 14.9% of damage ratio for fishing gears on catching the target fishes inhabiting on the rough sea bottom. Catches by water depth was the order of 300m below, 301-350m, 451-500m and 351-400m. It is thought that there was no problem on the mesh size because most of fishes caught from the experimental fishing was 30cm over.