• Journal of Plant Biotechnology
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• 제39권4호
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• pp.225-234
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• 2012

This report describes recent advances in tissue culture and genetic transformation of commercial Phalaenopsis. Recently, an importance of Phalaenopsis has been increased due to its popularity with beautiful flowers and is widely used for pot plants as well as cut-flower. Its use is rapidly enlarging in worldwide. Thus, demands for the release of new elite cultivars in Phalaenopsis have been increased. During the last several decades, some critical progresses have been made in tissue culture and genetic transformation in Phalaenopsis species. Cooperation with these biotechnological methods are supposed to promote the release of commercial Phalaenopsis cultivars in the near future. Until now, no technical review on tissue culture and genetic transformation in Phalaenopsis has been reported in Korea. Therefore, we inquired the brief history and techniques of tissue culture system in Korea.

• 한국식물병리학회지
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• 제14권5호
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• pp.536-542
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• 1998

Ten isolates of the orchid mycorrhizal fungi were isolated from the roots of Korean native orchid plants (Cymbidium goeringii) which inhabitate mainly in southern and western areas of Korea. The growth rates and color of the isolates in potato dextrose agar (PDA) were various. Microscopic observations of the hyphae isolated were identified as Rhizoctonia repens and R. endophytica var endophytica or their related species. R. repens was isolated from the roots of the Korean native orchids, but R. endophytica var endophyica was only isolated from the roots of the commercial orchids introduced from foreign countries. Also, the polymorephic patterns of genomic DNA extracted from selected isolates were compared with those of DNA extracted from the orchid mycorrhizal fungi isolated previously and similar band patterns were observed among those isolates. Five isolates of R. repens were selected and cultured at the oatmeal agar for investigating their symbiosis with orchid plants. The symbiotic specificity between orchid plants and isolated orchid mycorrhizal fungi was observe by growing orchids about six months in the greenhouse. The symbiotic responses of the commercial orchid plants with selected isolates were quite different form different isolates due to the genetic variations.

• 한국어업기술학회:학술대회논문집
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• 한국어업기술학회 2000년도 춘계수산관련학회 공동학술대회발표요지집
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• pp.233-234
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• 2000

Formalin, 37% formaldehyde, has been employed as a chemical for controlling ectoparasites and aquatic fungi responsible for infectious fish of diseases in hatcheries and culture facilities (Roberts, 1978; Schnick, 1991; Rach et al., 1997). Regarding the commercial usage of formaldehyde in the aquaculture industry, the U.S. Food and Drug Administration (FDA) approved three commercial products as parasiticides in a species-specific manner: Paracide-F and Formalin-F for bluegill, catfish, largemouth bass, salmon, and trout and Parasite-S for all finfish (FDA, 1998). Withdrawal time for these products was legally zero when used as permitted under the regulations. With the increased production of cultured fish in Korea, such as olive Hounder Paralichthys olivaceus and black rockfish Sebastes schlegeli, application of formalin to diseased fish has become more frequent. Moreover, there is still some concern about environmental exposures caused by effluents from fish culture facilities. The purpose of the present study was to evaluate residues in fish resulting from therapeutic usage of formalin in the aquaculture industry and to document the rate of disappearance of formaldehyde in seawater treated with formalin. (omitted)

• Mycobiology
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• 제41권4호
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• pp.252-255
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• 2013

Fungal pathogens have caused severe damage to the commercial production of Pleurotus eryngii, the king oyster mushroom, by reducing production yield, causing deterioration of commercial value, and shortening shelf-life. Four strains of pathogenic fungi, including Trichoderma koningiopsis DC3, Phomopsis sp. MP4, Mucor circinelloides MP5, and Cladosporium bruhnei MP6, were isolated from the bottle culture of diseased P. eryngii. A species-specific primer set was designed for each fungus from the ITS1-5.8S rDNA-ITS2 sequences. PCR using the ITS primer set yielded a unique DNA band for each fungus without any cross-reaction, proving the validity of our method in detection of mushroom fungal pathogens.

• Weed & Turfgrass Science
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• 제2권2호
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• pp.164-169
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• 2013

Alternatives to commercial chemical herbicide are currently being searched and tested due to the numerous adverse effects of commercially available herbicides to the environment. Barnyardgrass (Echinochloa crusgalli) is an important weed species around the world, especially in paddy rice fields. This study focuses on the favorable effects of mixing pyroligneous acids with commercial liquid herbicides. Seedlings were transplanted and grown under greenhouse conditions. The effect of treatment time or leaf-stage on herbicide-pyroligneous acid efficacies was checked, coupled with isolation and quantification of biochemical compounds. Results revealed that herbicide treatment at early post emergence (2~3 leaf stage) of Echnochloa crus-galli leads to effective control. Both liquid herbicides affected fatty acid, protein, and amino acid syntheses as reflected on their contents. The influence of wood vinegar (WV) or rice vinegar (RV) on these compounds was not thoroughly verified due to lack of information on the pyroligneous products. We observed that mixing WV or RV with BCB (bentazone + cyhalof-butyl) gives more favorable results than BUC (butachlor + clomazone), mixed with WV or RV. The result would indicate the potential of mixing pyroligneous acid in reducing herbicide application rate.

• 식물병연구
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• 제26권4호
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• pp.279-282
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• 2020

In 2019, symptoms of Botrytis mold on the peony (Paeonia lactiflora Pall.) 'Sarah Bernhardt' were observed during a survey of the commercial greenhouses of Gangjin County, South Korea. The initial symptoms, small brown spots, were observed mainly at the leaf margins. The lesions extended to the interior of leaves forming irregular spots in which abundant conidia developed. Fungal colonies were obtained from surface-sterilized tissue excised from growing edges of the lesions that were transferred to potato dextrose agar. Melanized irregular sclerotia were formed in these colonies after 40 days at 8℃. Molecular phylogeny based on sequences of genes for glyceraldehyde-3-phosphate dehydrogenase, heat-shock protein 60, and RNA polymerase subunit II were highest for the PBC-2 isolate to the type strains of Botrytis cinerea, rather than other Botrytis species associated with peony diseases. Following Koch's postulates, healthy Sarah Bernhardt plants were inoculated with a foliar application of conidial suspensions of the isolate PBC-2. Following incubation under humidity with a 12 hr photoperiod for 7 days, symptoms developed on the leaf margins that were identical to those observed in the greenhouses. This study is the first report of Botrytis blight caused by B. cinerea on peonies grown in commercial greenhouses in South Korea.

• 한국축산식품학회지
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• 제41권1호
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• pp.85-94
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• 2021

A pig-specific real-time PCR assay based on the mitochondrial ND5 gene was developed to detect porcine material in food and other products. To optimize the performance of assay, seven commercial TaqMan master mixes and two real-time PCR platforms (Applied Biosystems StepOnePlus and Bio-rad CFX Connect) were used to evaluate the limit of detection (LOD) as well as the PCR efficiency and specificity. The LODs and PCR efficiencies for the seven master mixes on two platforms were 0.5-5 pg/reaction and 84.96%-108.80%, respectively. Additionally, non-specific amplifications of DNA from other animal samples (human, dog, cow, and chicken) were observed for four master mixes. These results imply that the sensitivity and specificity of a real-time PCR assay may vary depending on master mix and platform used. The best combination of master mix and real-time PCR platform can accurately detect 0.5 pg porcine DNA, with a PCR efficiency of 100.49%.

• 한국응용곤충학회지
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• 제48권3호
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• pp.369-375
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• 2009

상용화된 Trichogramma sp. Nabis101은 알 기생봉으로 대상 기주 범위가 넓어 해충 방제용으로 농생태계에 방사되고 있다. 더욱이 최근에 미생물농약과 혼용 기술이 개발되어 이들의 적용 확대가 기대되고 있다. 그러나 아직 이 기생봉의 정확한 종 동정이 되어 있지 않다. 또한 비교적 불규칙적 수요에 대응하여 생존력과 기생 능력 차이 없이 기생봉을 저장할 수 있는 기술이 요구되고 있다. 본 연구는 이 기생봉의 internal transcribed spacer (ITS)의 염기서열을 결정하였다. 이를 토대로 이 기생봉의 종 동정을 실시한 결과 T. brasiliensis와 가장 높은 유사성을 나타냈다. 이 기생봉의 저장은 기생 초기의 기주 알을 $10^{\circ}C$에서 보관하는 기술이 결정되었다. 이 조건에서 기생봉은 5주 동안 생존력, 성비, 수명 및 기생능력의 큰 저하 없이 유지될 수 있었다.

• 한국수산과학회지
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• 제25권4호
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• pp.265-281
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• 1992

1. 본 조사 연구는 낙동강 하구일대의 연체동물과 갑각류를 대상으로 하였으며, 재료는 1991년 8월-1991년 11월의 기간동안 물금-다대포-가덕도 일대와 죽림천, 맥도강에 이르기까지 총 30개 지점에서 직접 채집한 표본들과 관찰기록을 근거로 하였다. 2. 본 조사에서 채집, 동정된 연체동물은 이 지역에서 처음 출현한 2종을 포함하여 2강 6목 21과 31종이었으며 갑각류는 처음으로 채집된 6종을 포함하여 3아강 51 28과 50종이었다. 3. 본 조사연구 결과와 이전의 문헌상의 기록을 포함하면 낙동강 하구 일대에서 확인된 연체동물과 갑각류는 각각 3강 9목 37과 63종, 3아강 5목 51과 138종이 되며, 이들의 채집기록을 8개의 구역에 따라 하구언 완공 전후로 구분하여 비교하였다. 4. 본 조사기간 동안 담수역인 하구언 이북의 낙동강 본류에서는 갑각류는 전혀 채집되지 않았으며 연체동물도 종수와 개체수가 크게 감소하여 담수종들의 정착이 이루어지지 않은 것으로 추측된다. 5. 하구언 이남의 을숙도 동안과 다대포에 이르는 강안의 지역은 석조제방공사로 인하여 혈거성 게류를 포함하는 조간대 종들이 거의 사라졌다. 6. 하구의 전 수역에 걸쳐 재첩과 반지락을 비롯한 식용패류는 하구언 완공 이전에 비해 개체수가 현저히 감소되었다. 담수종인 동남참게(Eriocheir japonicus)는 낙동강 전 수역과 인접한 하천에서 완전히 사라진 것으로 믿어진다.

• Journal of the Korean Wood Science and Technology
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• 제14권4호
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• pp.40-46
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• 1986

국내산(國內産) 16수종재의 철오염(鐵汚染), 알칼리오염(汚染) 산오염(酸汚染), 태양광(太陽光)에 의한 변색을 측정한 결과를 요약하면 다음과 같다. 1. 철오염(鐵汚染)이 심(甚)한 수종(樹種)은 일본잎갈나무, 신갈나무, 느티나무, 왕버섯나무등 이었고, 적은 수종(樹種)은 참오동나무, 리기다소나무등 이었다. 2. 국내산수종(國內産樹種)은 알칼리오염(汚染)이 적은 평이었고, 알칼리오염(汚染)이 낮은 수종은 젓나무, 음나무, 현사시, 고로쇠나무, 리기다소나무, 신갈나무, 자작나무, 왕벗나무, 느티나무, 이태리포플러, 잣나무, 참오동나무 등이었다. 3. 산오염(酸汚染)이 심(甚)한 수종(樹種)은 본(本) 실험(實驗)에서 사용재중(使用材中)에는 나타나지 않았고, 낮은 수종은 리기다소나무, 오동나무, 잣나무, 신갈나무, 음나무, 고로쇠나무 등 이었다. 4. 태양광(太陽光)에 의한 변색은 본(本) 실험(實驗)에서 사용(使用)한 공시재중(供試材中)에는 변색의 정도가 높은편으로서 변색이 심(甚)한 수종은 일본잎갈나무, 고로쇠나무, 참오도나무, 이태리포플러, 소나무, 아까시나무, 젓나무, 현사시, 느티나무, 왕벗나무, 리기다소나무 등이었으며, 태양광에 의한 변색이 낮은 수종(樹種)은 오리나무로서 국내산재 16수종이 태양광에 의한 변색의 정도가 높은 것이 특징이었다. 일반적(一般的)으로, 한국산(韓國産) 수종(樹種)은 태양광(太陽光)과 철오염(鐵汚染)에 의한 정도가 높은 편이었고, 알칼리오염(汚染)과 산오염(酸汚染)에 의한 변색은 낮은 편에 속하였다.