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Properties and Utilization of Undigested Peptides in Anchovy Sauces 1. Use of Undigested Peptides as a Quality Parameter of Anchovy Sauces (멸치 액젓 중에 존재하는 미분해 펩티드의 특성과 이용 1. 미분해 펩티드를 이용한 멸치 액젓의 품질 지표 설정)

  • CHOI Young Joon;KIM Se-Hwan;IM Yeong-Sun;Kim In-Soo;KIM Dong-Su;CHO Young-Je
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.31 no.3
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    • pp.386-392
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    • 1998
  • An objective index for quality estimation of anchovy sauce, our traditional seafood, has been required because inadequate standard causes dispute about quality estimation. Qualities of anchovy sauce made by traditional method and commercial anchor sauce products were compared by investigating their proximate compositions, total nitrogen contents, amino acid contents, development and level of a specific peptide on SDS-polyacrylamde gel electrophoresis (SDS-PAGE) and isoelectric point (pI) of the specific peptide. Also, pIs of IEF were attemped to identify fish species of salt-fermented sauces. The 55,600 and 46,900 dalton of peptide band were identified in all experimental samples. Especially, the area of 55,600 dalton was closely correlated with total nitrogen contents. Also, specific pIs in accordance with fish species were identified. The results suggest that correlation between area of 55,600 dalton and total nitrogen contents are used of index for quality estimation, and specific pIs are used of fish species identification.

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The Effect of Additives to the Texture of Kelp Blade (다시마 조체의 연화를 위한 첨가제의 효과)

  • JEONG In-Hak;LEE Kyung-SEON;LEE Kang-Ho
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.27 no.2
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    • pp.149-154
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    • 1994
  • The hardness of kelp(Laminaria japonica) blade is a major deterrent to mass consumption in spite of its value as food. For improvement of kelp products as a special product of 'Donghae', the effect of additives on softening of kelp blade was studied. Kelp was composed of $34.74\%$ ash, $50.5\%$ carbohydrates, $4.81\%$ lipids and $9.98\%$ proteins on a dry weight basis. Glutamic and Aspartic acid are the principal free amino acids with percentage compositions of $2340mg\%\;and\;1650mg\%$ respectively of the $4719mg\%$ of total free amino acids. The softening effects of acetic acid and commercial mixed polyphosphate are more effective than that of monosodium carbonate. Boiling for more than 1hr was necessary for adequate softness of kelp blade in $0.3\%$ acetic acid solution. But, the kelp blade were softened more satisfactorily to 0.165kg/mm for only 30 min boiling in $0.2\%$ phosphate solution, and it is equal to boiling in $0.3\%$ acetic acid for 1hr.

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Optimization of Peptide Production from Leg Meat of Yeonsan Ogae by High Hydrostatic Pressure and Protein Hydrolytic Enzyme and Its Characteristic Analysis (고압처리와 단백질 분해효소를 이용한 연산오계 다리육 펩타이드 생산 최적화 및 특성 분석)

  • Ha, Yoo-jin;Kim, A-Yeon;Yoo, Sun-Kyun
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.17 no.7
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    • pp.182-191
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    • 2016
  • The purpose of this research was the optimization of protein hydrolysate production using a commercial enzyme bromelain 1200 derived from the leg of Yeonsan Ogae by response surface methodology. Yeonsan Ogae has long been known as supporting health and high efficacy treatment. In recent days, as the efficacy of functional peptides becomes more known, optimization of oligopeptide production and its characteristics from Ogae leg meat has been performed. Response surface methodology was performed for optimization of enzyme hydrolysis. The process was varied in pressure (30 to 100 MPa), time (1 to 3 h), and substrate concentration (10 to 30%). The degree of hydrolysis, amino acids, and molecular weight of products were analyzed. The optimum conditions were determined to be a pressure of 100 Mpa, time of 3 h, and substrate concentration of 20%. Under optimized conditions, degree of hydrolysis was 34.10%. The average molecular weight of protein hydrolysates was less than 1,000 Da. Major amino acids were leucine, lysine, alanine, glutamic acid, and phenylalanine.

Processing Optimization of Seasoned Laver Pyropia yezoensis Using Seasoning Sauce with Conger Eel Conger myriaster (붕장어(Conger myriaster) 시즈닝을 활용한 조미김(Pyropia yezoensis)의 제조공정 최적화)

  • Kim, Do Youb;Kang, Sang In;Lee, Chang Young;Kim, Hye Jin;Lee, Jung Suck;Heu, Min Soo;Kim, Jin-Soo
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.53 no.3
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    • pp.368-381
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    • 2020
  • This study was conducted to optimize the processing conditions of seasoned laver Pyropia yezoensis with conger eel Conger myriaster seasoning sauce (CES) using response surface methodology (RSM). The RSM program results for bonesoftness showed that the optimum independent variables based on the dependent variables (Y1, lipid removal rate; Y2, texture; and Y3, sensory fish odor score) were 431.0% for X1 (water amount), 115.6℃ for X2 (retort-operated temperature), and 50.1 min for X3 (retort-operated time). The RSM program results for the CES blend showed that the optimum independent variables (X1, amount of bone-softened conger eel by-products; X2, mixed sauce amount; and X3, starch amount) based on the dependent variables (Y1, amino-N; Y2, Hunter redness; and Y3, drying time) were 44.8% for A (pre-treated conger eel by-product), 36.0% for B (mixed sauce), and 19.2% for C (starch). The RSM program results for seasoned laver with CES showed that the optimum independent variables based on the dependent variables (Y1, water activity; Y2, Hunter yellowness; and Y3, overall acceptance) were 5.0% for X1, (CES amount), 313.8℃ for X2 (roasting temperature), and 6.0 s for X3 (roasting time). The seasoned laver with CES prepared under the optimum conditions was superior to commercial seasoned laver in terms of overall acceptance.

Studies on Discoloration of 16 Commercial wood Grown in Korea (한국산(韓國産) 유용목재(有用木材)의 변색(變色)에 관한 연구)

  • Kim, Moom-Kyu;Chung, Dae-Kyo
    • Journal of the Korean Wood Science and Technology
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    • v.14 no.4
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    • pp.40-46
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    • 1986
  • Change of color of woods is a trouble frequently found in decorative wood products. In this paper, studies were carried out on discoloation sensitivities of wood specimens to iron (0.1%, $FeCl_3{\cdot}6H_2O$), alkali (pH12.0, NaOH), acid (pH 1.0 $C_2H_2O_4$) and exposing to sunlight (40 hrs.). Four soft-woods and eleven hard-woods grown in Korea were used in this test. The results obtained were as follows: 1. In iron stain, strong discolored wood species were Larix leptolepis, Quercus mongolica. Zelkova serrata and Prunus yedoensis, and light discolored were Paulownia lomentosa and Pinus rigida. 2. In alkali stain. light discolored wood species were Abies holophylla, Kalopanax pictum, Populus ${\times}$ albaglandulosa, Acer mono, Pinus rigida, Pinus densiflora, Quercus mongolica, Betula platyphylla var. japonica, Prunus yedoensis, Zelkova serrata, Populus euramericana, Pinus koraiensis and Paulownia tomentosa, but there was no wood species of strong discolored compared with the color difference values of other reports. 3. In acid stain, light discolored wood species were Pinus rigida, Paulownia tomentosa. Pinus koraiensis, Quercus mongolica, Kalopanax piclum and Acer mone, but there was no strong discolored species compared with the color difference values of other reports. 4. In exposing to sunlight, strong discolored species were Larix leptolepis, Acer mono, Paulownia tomentosa, Populus euramericana, Pinus densijlora, Robinia pseudoacacia, Abies holophylla, Populus ${\times}$ albaglandulosa, Zelkova serrata, Prunus yedoensis and Pinus rigida, and light discolored was Alnus japonica. In general, it was shown that Korean wood species were susceptible to change of color by exposing to sunlight and iron stain compared with by alkali stain and acid stain.

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Development of Depth-Damage Function by Investigating Flooded Area with Focusing on Building Damage (피해설문조사 기반의 도시지역의 침수심별 피해 추정함수 개발 -건물피해를 중심으로-)

  • Kim, Sang Ho;Kim, Byung Sik;Lee, Chang Hee;Chung, Jae Hak
    • Journal of Korea Water Resources Association
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    • v.47 no.8
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    • pp.717-728
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    • 2014
  • In this study, we developed a depth-damage function based on flood damage survey with focusing on building damage in urban area. We designed items for the questionnaire survey to develop a depth-damage function which estimates the amount of damage based on inundation depth targeting Dongducheon, Korea, which has experienced severe inundation damage due to significant flooding in July 2011. Based on the survey of the area, we developed a depth-damage function and used this to estimate the real amount of damage on buildings in the inundation area. To assess the damage on buildings, we categorized buildings into two groups; namely residential buildings and commercial buildings. Also, in order to calculate the real amount of damage caused by flooding, properties and detailed damaged items were sub-divided into two groups for the survey; facilities loss (wall paper, floor paper, painting, electrical facilities, and boilers) and furnishing loss (furniture, electronic products, and daily necessities. We expect this study on the process for developing depth-damage function and on the investigation research for flooded area to help in the efficient implementation of all kinds of disaster management policies and the attainment of a society safe from disaster.

In vitro Antioxidant Activities of Cocoa Phenolics (코코아 폴리페놀 성분의 in vitro 항산화 활성)

  • Jeong, Chang-Ho;Choi, Gwi-Nam;Kwak, Ji-Hyun;Kim, Ji-Hye;Choi, Sung-Gil;Shim, Ki-Hwan;Heo, Ho-Jin
    • Food Science and Preservation
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    • v.17 no.1
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    • pp.100-106
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    • 2010
  • The contents and antioxidant activities of various polyphenolic fractions from commercial cocoa powder were investigated. Total phenolics extracted by 0.01 M HCl, ethyl acetate, and acidic methanol, from an 80% (v/v) methanolic extract of cocoa, were 19.25, 202.24, and 295.83 mg/g, respectively. Each fraction was capable of scavenging DPPH radicals in a concentration-dependent manner. Among the various fractions, the acidic methanol fraction had the highest ABTS radical scavenging ability. The reducing power of the 0.01 M HCl, ethyl acetate, and acidic methanol fractions were 0.44, 3.15, and 3.87, respectively, at 1,000 ${\mu}g/mL$. Antioxidant activities, as assessed by $\beta$-carotene bleaching and linoleic acid autoxidation, of the various phenolic fractions from cocoa decreased in the order acidic methanol > ethyl acetate > 0.01 M HCl. Inhibition of $\beta$-carotene bleaching mediated by the acidic methanol fraction was similar to that of the ethyl acetate fraction. The values were 60.18% and 58.97%, respectively, at 1,000 ${\mu}g/mL$. Therefore, cocoa and cocoa-containing products may contain natural antioxidants useful in prevention of diseases associated with aging.

A Study on the Successful Strategy for DAB : Focused on DAB Strategy in UK (DAB 성공전략에 관한 연구 - 영국 DAB전략을 중심으로 -)

  • Bae, Hong-Kyun
    • Korean Business Review
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    • v.18 no.2
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    • pp.133-149
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    • 2005
  • DAB is meeting with mixed success. Denmark is performing similarly to the UK thanks to the innovation of the public broadcaster but where DAB is not so strong, the problem can usually be pointed at the lack of enabling regulation. The UK's example, whilst not appropriate everywhere, has valuable lessons including licensing incentives for existing analogue commercial broadcasters and a market-building obligation on the national multiplex licensee. Despite the obvious success of DAB, it would be an omission to leave some of the continued criticisms unanswered, whether of its slow start or the underlying technology. True, there was much over-optimism in the mid-1990s, coupled with unrealistic promises and expectations of receiver pricing and consumer take-up. Governments across Europe have legislated for DAB digital radio in a variety of ways but few as successfully as the UK. It is essential that both public and private broadcasters are encouraged equally to participate in digital radio. The UK is fortunate because, for the last 10 years, there has been a progressive government policy towards digital broadcasting. The 1996 Broadcasting Act set out a full licensing regime for both digital television and digital radio. The 1996 UK legislation contained a number of key elements which have been cornerstones of its success. DAB digital radio began test broadcasting around ten years ago but it has not been a universal consumer success across Europe. In the UK, however, digital radio receivers are one of the fastest-selling consumer electronics products and sales have overtaken those of analogue radios. Why has the UK succeeded with DAB digital radio when other European countries have yet to see their markets take off? This article explains what steps the UK took to make DAB digital radio a success.

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A Critical Study on Arthur C. Danto's' Philosophizing Art' (단토의 '철학하는 예술' 개념에 대한 비판적 고찰)

  • Kim, Baik-Gyun
    • The Journal of Art Theory & Practice
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    • no.10
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    • pp.183-202
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    • 2010
  • The term "philosophizing art" was coined by Arthur Danto, who tried to define new forms of art, especially Andy Warhol's pop art appeared in New York after 1960's, which could not be explained by traditional concept of "representation". As Danto said "the term 'philosophizing art' is unclear, whether art discusses philosophical issues or art is the object of philosophic discussions", it does not seem like Danto himself had a specific idea when he used this term. The background for Danto coining this term derives from the fact that the old art concept such as denotation and connotation could not fully explain phenomenalistic aspects of concept art which appeared frequently at that time. Many articles in his book "philosophizing art", in which many of his criticism are included, mainly say that art begins philosophizing by dealing with not mimesis or representation but concepts. According to his argument, the history of western art, which has been consisted of mimesis and representation, has come to end when art is about physically embodied with meaning. Of course, as Danto say so, what goes to end is not art itself, but the narrative of art. It means master narrative saying art should be shown different from nature or artificial daily product is over. Danto could not find principals of mimesis and representation which had been main logic in the western art history, when he saw Andy Warhol's Brillo Boxes at Stable Gallery, New York in 1964. Danto questioned "if we can not distinguish Brillo box's artistic aspects visually from other factory-made products, how can we distinguish art from non-art", By answering those questions, he discovered two facts which made him realize the end of Art: One is there is no special way in which works of art have to be shown or has to exist. Therefore, art history has proven that commercial boxes, trashes and files of underwear can be a work of art. The other is we have fully recognized it at the end of 20thcentury. Danto confessed that through Brillo Boxes, he realized the works of art are decided by something can not be seen by eyes, not by distinguished differences by looking at it. This thesis is trying to show personal understanding about art, philosophy and discourses surrounding them and to figure out how Dante opened a new world to art criticism by using new definitions such as 'end of art' and 'philosophizing art' which Danto used to explain inner aspects of art.

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Fate and Bioaccumulation of Zinc Oxide Nanoparticles in a Microcosm (산화아연 나노물질의 미소생태계 내 거동 및 생물축적)

  • Kim, Eunjeong;Lee, Jae-woo;Jo, Eunhye;Sung, Hwa Kyung;Yoo, Sun Kyoung;Kim, Kyung-tae;Shin, Yu-jin;Kim, Ji-eun;Park, Sun-Young;Eom, Ig-chun;Kim, Pilje
    • Journal of Environmental Health Sciences
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    • v.43 no.3
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    • pp.194-201
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    • 2017
  • Objectives: Zinc oxide nanoparticles (ZnO NPs) are widely used in various commercial products, but they are exposed to the environment and can induce toxicity. In this study, we investigated the environmental fate and bioaccumulation of ZnO NPs in a microcosm. Methods: The microcosm was composed of water, soil (Lufa Soil 2.2) and organisms (Oryzias latipes, Neocaridina denticulata, Semisulcospira libertina). Point five and 5 mg/L of ZnO NPs were exposed in the microcosm for 14 days. Total Zn concentrations were measured using an Inductively Coupled Plasma Mass Spectrometer (ICP-MS) and intracellular NPs were observed using Transmission Electron Microscopy (TEM). Results: In the initial stages of exposure, the Zn concentrations in water increased in all exposure groups and then decreased, while the Zn concentration in soil increased after three hours for the 5 mg/L solution. Zn concentrations also showed increasing trends in N. denticulata and S. libertina at 0.5 and 5 mg/L, and in O. latipes at 5 mg/L. Accumulation of NPs was found in the livers of O. latipes and hepatopancreas of N. denticulata and S. libertina. Conclusions: In the early stages of exposure, ZnO NPs remained in the water, and then were transported to the soil and test species. Unlike other species, total Zn concentrations in N. denticulata and S. libertina increased for both 0.5 mg/L and 5 mg/L. Therefore, ZnO NPs were more easily accumulated in zoobenthos than in fish.