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Exposure Assessment of Total Aflatoxin in Foods (식품중 총 아플라톡신의 노출량 평가)

  • Suh, Jung-Hyuk;Sho, You-Sub;Park, Seong-Soo;Choi, Woo-Jeong;Lee, Jong-Ok;Kim, Hee-Yun;Woo, Gun-Jo;Oh, Keum-Soon
    • Korean Journal of Food Science and Technology
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    • v.39 no.1
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    • pp.25-28
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    • 2007
  • A survey of total aflatoxin levels was conducted on 565 food samples (cereals, nuts, etc) collected in commercial markets. The determination of aflatoxins ($B_{1}$, $B_{2}$, $G_{1}$ and $G_{2}$) was performed using HPLC with fluorescence detector. The Limit of Detections (LODs) of the B group and G group were 0.05 ng/g and 0.07 ng/g, respectively. In addition, recoveries of rice, peanut butter, and red pepper flour were satisfactory. Total aflatoxin was detected 27 samples(4.8%) out of 565 samples. Incidence ratios in cereals, nuts, processed products, and other foods were 0.2, 0.4, 3.0 and 1.2%, respectively, but aflatoxin was not detected in pulse and dried fruits. The daily intake of total aflatoxin using food intakes was 0.04 ng/kg bw/day.

Fibrinolytic, Immunostimulating, and Cytotoxic Activities of Microbial Strains Isolated from Kochujang (고추장 분리 균주의 혈전용해능, 면역활성능 및 세포독성 효과 조사)

  • Seo, Mi-Young;Kim, Seung-Ho;Lee, Cheol-Ho;Cha, Seong-Kwan
    • Korean Journal of Food Science and Technology
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    • v.39 no.3
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    • pp.315-322
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    • 2007
  • This study was carried out to investigate the functional activities of microorganisms isolated from kochujang, such as fibrinolytic, immunostimulating, and cytotoxical activities, and to apply these microorganisms to kochujang products. Ninety-one microbial strains with proteolytic activity were selected from 294 strains isolated from traditional and commercial kochujang. Three strains (TPP 0014, TPP 6013, and TPP 6015) with high fibrinolytic activity were tested for their immunostimulating and cytotoxical activities. For the assessment of macrophage activation, cytokines such as tumor necrosis factor, $interleukin-1{\alpha}$ and nitrogen oxide were measured with the murine macrophage cell line RAW 264.7. In addition, the cytotoxical activities of the three strains were examined by MTT assay on the colon cancer cell line SNU-C4 and normal cell line CHO-K1. Using an API identifying kit, two of the microbial strains (TPP 0014 and TPP 6015) were identified as Bacillus stearothermophilus and the other strain (TPP 6013) was identified as B. amyloliquefacience.

Effects of fermentation by the commercial starter ABT-5 on the flavor and antioxidant activities of dark chocolate (복합 유산균 스타터 ABT-5를 이용한 발효 다크 초콜릿의 항산화 활성 및 향기 성분)

  • Koh, So Yae;Ryu, Ji-Yeon;Kim, Hyeon A;Kim Cho, Somi
    • Korean Journal of Food Science and Technology
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    • v.49 no.6
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    • pp.617-624
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    • 2017
  • Chocolate, one of the most popular confectioneries in the world, is known for its aromatic flavor and high antioxidant activities. In this study, we investigated the effects of fermentation with commercially available lactic acid bacteria, ABT-5, on the flavor and antioxidant activities of dark chocolate. During 24 h fermentation, pH decreased from 5.52 to 3.97 and total acidity increased from 0.51 to 1.85%, whereas total polyphenol and flavonoid contents as well as DPPH and ABTS radical scavenging activities remained unchanged. Furthermore, compared with control HepG2 cells treated with unfermented dark chocolate, those treated with the fermented dark chocolate showed significantly lower levels of reactive oxygen species and higher viability under $H_2O_2-induced$ oxidative stress. Finally, GC-MS and headspace GC-MS analysis detected 4-hydroxy-2,5-dimethyl-3(2H)-furanone and 2-furanmethanol, known to enhance flavor, in the fermented dark chocolate. Collectively, these results suggest that ABT-5-fermented dark chocolate could be utilized for developing value-added dark chocolate products.

A Study on the Accelerated Life Test of BLDC Motor in Ceiling Mounted Digital Signage Rotating System (천정 거치형 디지털 사이니지 회전 시스템의 BLDC모터 가속수명시험에 관한 연구)

  • Kim, Ki-Hong;Kwon, Soon-Hong;Kwon, Soon-Gu;Park, Jong-Min;Kim, Jong-Soon;Jung, Sung-Won;Choi, Won-Sik
    • Journal of the Korean Society of Industry Convergence
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    • v.21 no.3
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    • pp.141-147
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    • 2018
  • In a time when product development cycles are getting shorter and shorter, many companies are making efforts to develop products with high reliability in a short period of time, accelerated life test is widely used as a method to quickly evaluate reliability. Accelerated life test reduces the test life or the life of the product from the observed data by shortening the lifetime of the product or abruptly lowering the performance under the worse condition than the actual condition in order to shorten the test cost or the test time. In this paper, BL3640A-06P+RB35, DC12V model, which is used in the support device of an automatic rotation type digital signage, which display various information such as textures and images on a display screen in a public place or a commercial space, BLDC motors were subjected to a constant stress test and at the rotational speed of 1rpm, $180^{\circ}$ rotation and reverse rotation under actual use conditions, the stress was imposed on the rotating speed of 2rpm and the weight of the actual installed product from 22.2kgf to 10kgf were installed. The lifetime of the actual use environment condition is 23,545 hours and the rotation speed is accelerated. The life time of the acceleration condition with the additional weight is 1,380 hours. The acceleration factor is calculated as 17.06, the one year guarantee test day is 235 days to 14 days, of the period from 470 days to 28 days, and the third year from 704 days to 42 days. The test date of the BLDC motor was tested on the shortened test date, and the rotational speed and the current value were measured. It is found that there is no defect even if it operates as the test date corresponding to the specified one year warranty period and the 3 year accelerated life test which is experimented. Using the statistical technique of the regression analysis the expected time for the motor to defect to #4 samples was 20 years.

Carbon Dioxide Reforming of Methane over a Ni/KIT-1 Catalyst (Ni/KIT-1 촉매를 이용한 메탄의 이산화탄소 개질반응 연구)

  • Ryn, Seong-Yun;Ahn, Wha-Seung;Park, Sang-Eon
    • Applied Chemistry for Engineering
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    • v.9 no.7
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    • pp.1070-1078
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    • 1998
  • Thermodynamic analysis on carbon dioxide reforming of methane was performed using a computer program which can handle condensed species in the products, and the reforming experiments were conducted over $Al_2O_3$, $La_2O_3$, ZSM-5, MCM-41, KIT-1 supported nickel catalysts, and a commercial ICI 46-1. It was estabished that a system which consists of $CH_4$, $CO_2$, CO, $H_2$, $H_2O$, and C is appropriate for theoretical equilibrium calculations and addition of water vapor or oxygen was found to diminish the contribution of carbon dioxide in reforming. Silicate molecular sieve-supported catalysts such as Ni/ZSM-5, Ni/MCM-41, Ni/KIT-1 were effective for high $CH_4$ and $CO_2$ conversions as well as for high CO yield. Coke formation was suppressed when CaO was added as a promoter. Ni/Ca/KIT-1 which contains 10% Ni with 3% Ca showed conversion approaching equilibrium levels above $650^{\circ}C$ and maintained constant activity over 20 h. Despite increased space velocity, relatively high conversion and CO yield were observed.

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Apparent and standardized ileal nutrient digestibility of broiler diets containing varying levels of raw full-fat soybean and microbial protease

  • Erdaw, Mammo M.;Perez-Maldonado, Rider A.;Iji, Paul A.
    • Journal of Animal Science and Technology
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    • v.59 no.10
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    • pp.23.1-23.11
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    • 2017
  • Background: Although soybean meal (SBM) is excellent source of protein in diets for poultry, it is sometimes inaccessible, costly and fluctuates in supply. The SBM can partially be replaced by full-fat SBM, but the meals prepared from raw full-fat soybean contain antinutritional factors. To avoid the risk of antinutritional factors, heat treatment is always advisable, but either excessive or under heating the soybean could negatively affect the quality. However, the potential for further improvement of SBM by supplementing with microbial enzymes has been suggested by many researchers. The objective of this study was to evaluate the performance and ileal nutrient digestibility of birds fed on diets containing raw soybeans and supplemented with microbial protease. Methods: A $3{\times}2$ factorial, involving 3 levels of raw full-fat soybean (RFFS; 0, 45 or 75 g/kg of diet) and 2 levels of protease (0 or 15,000 PROT/kg) was used. The birds were raised in a climate-controlled room. A nitrogen-free diet was also offered to a reference group from day 19 to 24 to determine protein and amino acid flow at the terminal ileum and calculate the standardized ileal digestibility of nutrients. On days 10, 24 and 35, body weight and feed leftover were recorded to calculate the body weight gain (BWG), feed intake (FI) and feed conversion ratio (FCR). On day 24, samples of ileal digesta were collected at least from two birds per replicate. Results: When RFFS was increased from 0 to 75 g/kg of diet, the content of trypsin inhibitors was increased from 1747 to 10,193 trypsin inhibitors unit (TIU)/g of diets, and feed consumption of birds was also reduced (P < 0.05). Increasing RFFS level reduced the BWG from hatch 0 to 10 d (P < 0.01) and hatch to 24 d (P < 0.05). The BWG of birds from hatch to 35 was not significantly (P = 0.07) affected. Feed intake was also reduced (P < 0.05) during 0 to 35 d. However, protease supplementation improved (P < 0.05) the BWG and FCR during 0 to 24 d. Rising levels of RFFS increased the weight of pancreas (P < 0.001) and small intestine (P < 0.001) at day 24. Except for methionine, apparent and the corresponding standardized ileal digestibility of CP and AA were reduced (P < 0.01) by increasing levels of RFFS in diets. Conclusion: This study showed that some commercial SBM could be replaced by RFFS in broiler diets, without markedly compromising productivity. The AID and SID of CP and lysine were slightly improved by dietary supplementation of microbial protease.

Synergistic Interaction of Fungicides in Mixtures under Different Conditions of Dollar Spot Disease Caused by Sclerotinia homoeocarpa (잔디 동전마름병의 발생정도가 다른 골프장 그린 조건에서 살균제 혼용살포에 의한 상승적 방제 효과)

  • Chang, Seog-Won;Jung, Suk-Woo;Kim, Se-Hun;Park, Jong-Hyun;Lee, Ji-Young
    • Asian Journal of Turfgrass Science
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    • v.26 no.2
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    • pp.96-101
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    • 2012
  • Dollar spot caused by Sclerotinia homoeocarpa F.T. Benn. is a common and economically important turfgrass disease in South Korea. Fungicides were evaluated for control of dollar spot in creeping bentgrass golf course putting green. Commercial formulations of propiconazole, thiophanate-methyl, chlorothalonil, trifloxystrobin and boscalid were applied to plots of creeping bentgrass, each of the latter four fungicides was tank mixed with propiconazole at the same rates, and applied as treatments. The dollar spot severity in the nontreated plots of field A and B progressed toward peak diseases of 18.3 and 66.7% from 10 or 15 days after inoculation, respectively. Significant differences were detected among control values of the fungicides. Dollar spot control provided by boscalid was significantly greater than the other fungicides which showed low control values in a higher disease condition. No synergistic interactions, except propiconazole + thiophanate-methyl treated plot, were detected under a lower disease pressure. However, under a higher disease pressure, synergism was observed at all fungicide combinations, except a propiconazole and boscalid tank mixture. These data suggest turfgrass managers in golf course can take advantage of fungicide synergism to control dollar spot using the products and rates in this study.

Study on the Physical Properties of Alkoxysilane-based Stone Consolidants with Different Hydrolysis (알콕시 실란계 석조문화재 강화제의 가수 분해도에 따른 특성 연구)

  • Park, Soung-Jin;Won, Jong-Ok;Kim, Jeong-Jin;Do, Jin-Young;Kim, Sa-Dug
    • Journal of Conservation Science
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    • v.27 no.2
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    • pp.201-209
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    • 2011
  • While consolidants based on tetraethoxysilane (TEOS) have been widely used for the consolidation of decaying stone heritages, TEOS-based consolidants suffer from practical drawbacks, such as crack formation of the gel during the drying phase due to the developed capillary force. We have prepared new TEOS-based consolidants containing flexible (3-glycidoxypropyl) trimethoxysilane (GPTMS) in order to reduce capillary force development during gel drying. In this study, we have prepared TEOS/GPTMS-based consolidants containing ETEOS in order to improve the surface hydrophobisity. The physical properties of the TEOS/GPTMS/ETEOS solution with different hydrolysis were compared with those of the commercial products Wacker OH$^{(R)}$. The contact angle of the surface increased with the addition of the ETEOS, which is higher than that of Wacker OH$^{(R)}$. The sol-gel mechanism was manipulated by the degree of hydrolysis as well as the amount of ETEOS. The properties and the applicability of the developed consolidants for the decayed Korean granites are also investigated.

Experimental study on Light and Gas Pollution Resistance of Commercial Natural Pigments for Dancheong - Focucing on Korea, Japan and China Products - (시판 단청용 천연안료의 내광성·내공해성 실험 연구 -한국, 일본, 중국 생산 안료를 중심으로-)

  • Lee, Kyeong Min;Kim, Soon Kwan;Bae, Su Bin;Kim, Mi Jeong
    • Journal of Conservation Science
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    • v.31 no.4
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    • pp.443-455
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    • 2015
  • To verifying the stability of Natural pigments for Dancheong which is available on the market was carried out as light resistance and gas corrosion test. In this particular case, we will confirm the characteristics of the only pigments except of Dancheong technique and influence of substance. Medium using for coloring are animal glue and synthetic resin that widely used for Dancheong in currently. Selected to artificial light source is a xenon arc lamp, contaminated gas is nitrogen dioxide gas in experiment. Degree of degradation of pigment were compared by measuring the degree of color change. The result of light resistance test, mineral pigments were good; color is green and blue type, soil pigments also good; color is yellow ocher, white clay, red clay etc. Gamboge, unghwang, cinnabar, red lead were confirmed not good. The result of gas corrosion test, most of the natural pigments were good condition except the red lead. Generally, animal glue samples had better than a synthetic resin samples.

Moire Interferometry Measurement and Numerical Analysis for Hygroscopic Swelling of Al-Polymer Joint (Al-Polymer 접합체의 흡습팽창에 대한 모아레 간섭 측정 및 수치해석)

  • Kim, Kibum;Kim, Yong-Yun
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.15 no.6
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    • pp.3442-3447
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    • 2014
  • A simple method to evaluate the hygroscopic characteristics of polymer of microelectronic plastic package is suggested. To evaluate the characteristics, specimens were prepared, and the internally absorbed moisture masses were measured as a function of the absorbing time and calculated numerically. The hygroscopic pressure ratio was calculated by heat transfer analysis supported by commercial FEM code because the hygroscopic diffusion equation has the same form as the heat transfer equation. The moisture masses were then summed by the self developed code. The nonconductive polymers had quite different characteristics for the different lots, even though they were the same products. The absorbed moisture mass variations were calculated for several different characteristics, and the optimal curve of the mass variation close to experimental data was selected, whose solubility and diffusivity were affected by the hygroscopic characteristics of the material. The method can be useful in the industrial fields to quickly characterize the polymer material of the semiconductor package because the fast correspondence is normally required in industry. The weight changes in the aluminum-nonconductive-polymer joint due to moisture absorption were measured. The deformed system was also measured using the Moire Interferometry system and compared with the results of finite element analysis.