• Journal of Plant Biotechnology
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• 제45권4호
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• pp.347-356
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• 2018

Chionanthus retusus is a small deciduous tree that is widely used in landscaping due to its beautiful white spring flowers and ornamental value. Conventional propagation through seeds requires one to two years of breaking dormancy. The objective of this study was to determine the conditions of in vitro germination in C. retusus. In vitro embryo culture was carried out to investigate the effects of six factors: basal media (McCown Woody Plant Medium (WPM) and Murashige and Skoog (MS)); plant growth regulators (different combinations and concentrations of naphthaleneacetic acid (NAA), 6-Benzylaminopurine (BA), and gibberellic acid ($GA_3$)); embryo age (collected weekly beginning 36 days after fruit setting); low temperature pretreatment (storing $4^{\circ}C$ for 1, 2, 3, and 4 weeks); coconut additives (100, 200, and $300ml{\cdot}L^{-1}$); and genotype (grouping plants depending on their flowering nature). The basal medium used in this study was WPM with $2mg{\cdot}L^{-1-1}\;GA_3$, $20g{\cdot}L^{-1}$ sucrose, and $6g{\cdot}L^{-1}$ Agar. WPM medium mixed with $GA_3$, resulted in higher germination rate as compared to when using a combination of auxin and cytokinin. $GA_3$ at $2mg{\cdot}L^{-1}$ was the most effective of all combinations and concentrations of PGRs. WPM medium with $2mg{\cdot}L^{-1}GA_3$ resulted in better and faster germination (75.93%). Embryos collected at 57 days after fruit setting had the highest percent of germinated seeds (87.04%) while low-temperature pretreatment of fruits at $4^{\circ}C$ for two weeks produced the highest germination (95.37%). These results of this study could be an open ground for development of an efficient protocol for commercial production of the ornamental tree.

• Biomolecules & Therapeutics
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• 제30권2호
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• pp.184-190
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• 2022

Targeting the cystine/glutamate exchange transporter, system x_c^-, is a promising anticancer strategy that induces ferroptosis, which is a distinct form of cell death mediated by iron-dependent lipid peroxidation. The concentration of ⳑ-cystine in culture medium is higher than the physiological level. This study was aimed to evaluate the effects of ⳑ-cystine concentration on the efficacy of ferroptosis inducers in hepatocellular carcinoma cells. This study showed that treatment with sulfasalazine or erastin, a system x_c^- inhibitor, decreased the viability of Huh6 and Huh7 cells in a dose-dependent manner, and the degree of growth inhibition was greater in medium containing a physiological ⳑ-cystine concentration of 83 µM than in commercial medium with a concentration of 200 µM ⳑ-cystine. However, RSL3, a glutathione peroxidase 4 inhibitor, decreased cell viability to a similar extent in media containing both ⳑ-cystine concentrations. Sulfasalazine and erastin significantly increased the percentages of propidium iodide-positive cells in media with 83 µM ⳑ-cystine, but not in media with 200 µM ⳑ-cystine. Sulfasalazine- or erastin-induced accumulation of lipid peroxidation as monitored by C11-BODIPY probe was higher in media with 83 µM ⳑ-cystine than in media with 200 µM ⳑ-cystine. In contrast, the changes in the percentages of propidium iodide-positive cells and lipid peroxidation by RSL3 were similar in both media. These results showed that sulfasalazine and erastin, but not RSL3, were efficacious under conditions of physiological ⳑ-cystine concentration, suggesting that medium conditions would be crucial for the design of a bioassay for system x_c^- inhibitors.

• 원예과학기술지
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• 제30권1호
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• pp.34-41
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• 2012

본 연구는 새롭게 개발된 무기물인 phenolic foam 배지를 이용한 파프리카 파종용 플러그묘의 생산 가능성을 알아보기 위해 유리온실에서 수행하였다. Grodan 암면, UR 암면, phenolic foam LC, phenolic foam RC, phenolic foam 3813-4의 5종류 펠릿형 배지에 파프리카 'Fascinato'를 파종하였다. 종자는 식물생장상($25^{\circ}C$, 상대습도 90%, 암상태)에서 4-5일 동안 발아시켰고, 발아가 시작된 후에는 유리온실로 옮겨 두상관수로 양액을 관주하였다. 파프리카의 생육은 파종 20일 후에 측정하였다. 배지의 pH를 측정한 결과 Grodan 암면이 가장 높았고, EC는 phenolic foam 3813-4가 가장 높았지만 식물의 독성 증상은 관찰되지 않았다. 모든 배지에서 90% 이상의 발아율을 보였다. 초장, 경경, T/R률, 엽면적, 엽록소 함량은 암면 배지와 비교하였을 때 생육이 유사한 것으로 나타났다. 엽수, 최대근장, 지상부의 건물중은 유의적인 차이가 없었다. 전체적으로 phenolic foam 배지인 LC와 RC는 암면배지와 비교하여 유사한 생육을 보였다. 본 실험결과 파프리카 'Fascinato'의 상업적 규모의 육묘를 위해 암면을 신개발 물질인 페놀릭 폼으로의 대체 가능성을 확인하였다.

• 생물환경조절학회지
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• 제16권4호
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• pp.309-313
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• 2007

본 연구는 배지의 종류 및 부피가 절화 장미의 발근과 발근 후 생육에 미치는 영향을 알아보기 위하여 수행하였다. 실험은 유리온실의 철재 베드에 높이 1.5m의 아치형 터널을 설치하고 습도유지를 위하여 fog(200sec./5min. or 100sec./5min) 가습하고, 삽목초기에 차광률 55%의 한랭사로 차광하였다. 실험재료는 Rosa hybrida 'Red Sandra'와 'Little Marble' 두 품종을 사용하였다. 배지의 종류에 따른 발근 차이를 알아보기 위하여 10가지 배지(토실이 상토, 암면큐브, phenolic foam, perlite, vermiculite, 또는 perlite와 peatmoss 1:1, perlite와 coir 1:1, perlite와 rockwool 1:1, rockwool 1:1, rockwool과 peatmoss 1:1, rockwool과 coir 1:1, v/v)를 준비하여 삽목하였다. 발근배지의 부피에 따른 발근 및 생육의 차이를 알아보기 위하여 3가지의 배지(토실이 상토, 암면큐브, phenolic foam)를 각각 3가지 부피$(3{\times}3{\times}3cm,\;5{\times}5{\times}5cm,\;7{\times}7{\times}7cm)$에 삽목하였다. 배지의 종류를 달리한 실험은 두 품종 모두에서 Rockwool 1: Peatmoss 1 배지에서 발근과 생육에서 가장 좋은 결과가 나타났으며, 배지의 부피에 따른 발근율과 모든 생육은 배지의 용적이 커질수록 좋았다.

• 생물환경조절학회지
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• 제12권1호
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• pp.7-11
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• 2003

본 실험은 폐기된 유리물질을 재가공하여 입자화한 CGF(cellular glass foam)를 펄라이트 대체 혼합상토로 이용하기 위하여 수행되었다. CGF의 입자크기를2.0∼4.0 mm로 조절한 후 피트모스와 혼합하여 배지를 조제하였고 관수빈도에 따른 Lycopersicum esculentum 'Segye' 플러그묘의 생육을 비교히였다. CGF와 피트모스의 혼합비율은 25：75와 33：67(%, v/v)이였으며 관수빈도는 2일에 1회, 2회, 또는 3회로 하였고, 상업용 플러그 배지(토실이 배지)에 1일 I회 관수한 것을 대조구로 하였다. 파종 후 33일에 플러그묘의 초장, 경경, 줄기와 뿌리의 생체중과 건물중, 엽수, 엽면적, 엽록소 함량, 그리고 배지의 pH와 EC를 조사하였다. 배지 혼합비율에 따른 플러그묘의 전체 생육은 유의성이 낮았다. 관수빈도에 따른 경경, 엽수, 엽면적, 줄기와 뿌리의 생체중과 건물중, T/R 그리고 배지의 EC에서 유의적인 차이를 보였다 CGF와 피트모스를 25：75(%, v/v)로 혼합한 배지에 관수빈도를 높인 처리에서 묘의 생육이 가장 좋았다.

• 한국신재생에너지학회:학술대회논문집
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• 한국신재생에너지학회 2009년도 춘계학술대회 논문집
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• pp.857-860
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• 2009

This report examines the economic feasibility of a commercial 50,000 barrel per day direct/indirect coal liquefaction (DCL/ICL) facility to produce commercial-grade diesel and naphtha liquids from medium-sulfur bituminous coal. The scope of the study includes capital and operating cost estimates, sensitivity analysis and a comparative financial analysis. Based on plant capacity of 50,000BPD, employing Illinois #6 bituminous coal as feed coal the total capital cost appeared $3,994,858,000(DCL) and $4,942,976,000(ICL). Also, the internal rate of return of DCL/ICL appeared 13.27% and 12.68% on the base condition. In this case, coal price and sale price of products were the most influence factors. And ICL's payback period(6.8 years) was longer than DCL's (6.6 years). According to sensitivity analyses, the important factors on DCL/ICL processes were product sale price, feed coal price and the capital cost in order.

• 펄프종이기술
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• 제38권2호
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• pp.43-51
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• 2006

Starch is widely used as an adhesive material in the paper and corrugated board industry. The adhesion problem of corrugated board is categorized by two main parts called zipper board and white glue line. The object of this research is to investigate the factors affecting the adhesion problem by the gelatinization of various starch solutions and dynamic penetration properties measurements of various commercial base paper and handsheets. Flow property of starch solution is affected by sodium hydroxide addition and reaction level. Absorption property of commercial base papers and handsheets is affected by sizing and stock composition. Optimum bonding between top/bottom liners and corrugated medium is accomplished by acceptable flow viscosity of mixed starch solution and proper adsorption characteristic of base paper.

• 한국실내디자인학회논문집
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• 제17권6호
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• pp.128-136
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• 2008

It wouldn't be exaggerating to say that we're living in a "Brand age" these days. Brand is easily spotted anywhere around our surroundings, influencing us in a material and a moral way. BI(Brand Identity) which brings increase on sales by constructing and improving the brand image, are infect visually compressed Identity designs that will give the interior space that have applied it, superiority in building a unified image. Then, graphic plays an important role in expressing Identity designs visually. These days, interior space graphic is an emotional field that can materialize images, which became an important expressing medium that offers various images and creativity and show interior space's brand identity. In this manner, space graphic that applies identity designs, clearly states brand's intentions on space communication and users by designing company images or brand's emotional factors visually and dimensionally. Also, it escapes plane level, and gives space, dimensional and kinetic elements, which can give differentiation with other brand's space. These kinds of flexibility let space graphic be used efficiently in extending space identity.

• Journal of Applied Biological Chemistry
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• 제49권4호
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• pp.135-139
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• 2006

A alkalophilic strain, Bacillus licheniformis MH31 producing an alkaline protease was isolated from mine soil of Boryeong in Korea. Production of a high level of alkaline protease was achieved 42 h after incubation when the bacterium was grown at pH 9.0 and $35^{\circ}C$ in Horikoshi medium supplemented with 0.5%(w/v) starch and 1%(w/v) skim milk as carbon and nitrogen source, respectively. The molecular weight of partially purified enzyme was estimated to be 30 kDa by SDS-PAGE and its optimum pH was pH 10. The enzyme showed optimum temperature at $50^{\circ}C$, and was stable up to $60^{\circ}C$ after 1 h incubation. The protease was strongly inhibited by 1 mM of PMSF which was known well as strong inhibitor of serine proteases, but almost not inhibited by 5 mM of EDTA and 1,10-phenanthroline. When the protein hydrolysis products of 1% skim milk by partially purified protease was compared with available commercial proteases using HPLC analysis, most of hydrolysis products were detected below molecular weight of 10,000 and the hydrolysis ratio of purified enzyme was 24.8% lower than those(above 32%) of commercial proteases.

• 물과 미래
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• 제29권5호
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• pp.161-172
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• 1996

상용관 마찰계수 산정을 위해 개발된 기존의 Hazen-Williams 식, Colebrook-White 식과 유동훈(1995)이 제안한야 영점높이 평균법과 마찰계수 평균법의 산정식들을 비교 검토하였다. 간단한 관망에 대하여 관종류, 관경, 유속, 관길이를 변화시켜 수리조건을 구성하였으며, 네가지 상용관 마찰계수 산정식을 적용하여 구한 압력차이를 비교하여 각각의 산정식들이 가지는 특징을 논하였고, 입력자료의 변화에 따라 마찰손실이 어떠한 거동을 하는지 알아보았다. 관경을 소형관 및대형관으로 변화시켰을 때의 분석결과와 중형관일 때의 분석결과를 세 종류의 강관에 대하여 비교하였고, 유속 변화와 압력 변화와의 비교를 통하여 기존 상용관 마찰 계수 산정식의 문제점에 대하여 논하였다.