• Title/Summary/Keyword: Combined Treatment

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LC-MS Analysis According to the Combined Treatment of Paenibacillus yonginensis DCY84T and Silicon in Rice

  • Yo-Han Yoo;Mee Youn Lee;Yeon-Ju Kim;Eok-Keun Ahn;Ki-Hong Jung
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2022.10a
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    • pp.232-232
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    • 2022
  • We reported in our recent studies that the combined treatment of Paenibacillus yonginensis DCY84T (DCY84T) and Silicon (Si) promotes initial plant growth and increases resistance to biotic and abiotic stress. To understand the molecular background of these phenotypes, Liquid Chromatography Mass Spectrometry (LC-MS) analysis was performed, and it was confirmed that unsaturated fatty acid metabolites such as oleic acid and linoleic acid decreased in response to the combined treatment of DCY84T and Si. The stearoyl-acyl carrier protein desaturase (SACPD) introduces the cis double bond into the acyl-ACPs at C9, resulting in the production of unsaturated fatty acid. We identified OsSSI2 encoding SACPD in rice and found that the expression of OsSSI2 was reduced under DCY84T and Si treatment. Furthermore, qRT-PCR analysis revealed that the expression of OsWRKY45, which is downstream of OsSSI2, was upregulated in response to DCY84T and Si treatment. These results enable the speculation that activation of the salicylic acid (SA)-responsive gene, OsWRKY45, may contribute to enhancing biological stress resistance. Based on this, we propose a probable model for the rice defense pathway following DCY84T and Si treatment. This model retains a WRKY45-dependent but NH1(NPR1)-independent SA signaling pathway.

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The Application of High-Intensity Ultrasound on Wet-Dry Combined Aged Pork Loin Induces Physicochemical and Oxidative Alterations

  • Yu-Min Son;Eun-Yeong Lee;AMM Nurul Alam;Abdul Samad;Md Jakir Hossain;Young-Hwa Hwang;Jeong-Keun Seo;Chul-Beom Kim;Jae-Ha Choi;Seon-Tea Joo
    • Food Science of Animal Resources
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    • v.44 no.4
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    • pp.899-911
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    • 2024
  • This research investigated the synergic outcome of high intensity ultrasound (HIU) treatment and wet-dry combined aging (WDCA) on physiochemical characteristics and lipid oxidation during refrigerated storage to ameliorate pork meat's quality and shelf life. The CIE b* values, cooking loss (CL %), and pH of the HIU treated samples were higher than those of the control over the aging period. They were significantly (p<0.05) modified by the aging period and ultrasound (US) treatment. However, the released water (RW %) and moisture were not significantly influenced by US treatment (p>0.05). The Warner-Bratzler shear force of HIU-treated samples was lower over control values except in 7-14 d, and it showed a significant difference between control and US treatment according to the significance of HIU (p<0.05). The thiobarbituric acid reactive substance of HIU-treated samples was significantly higher (p<0.05) than control values over the aging period. These results suggested that HIU treatment and WDCA showed a synergistic effect of maximizing the tenderness, but lipid oxidation was higher than before ultrasonic treatment. In agreement with this, the most favorable approach would involve implementing wet aging for a period of two weeks followed by dry aging for a period not exceeding one week after the application of HIU.

A Clinical Case of Vision Training for the Pediatric Intermittent Exotropia Combined with Vertical Deviation (수직편위를 동반한 간헐성외사시 소아에 대한 시기능 훈련 후 임상 사례)

  • Lee, Chang-Seon;Kim, Jae-Do;Son, Jeong-Sik;Kim, Dae-Nyoun;Jang, Woo-Yeong;Kim, Ki-Hong
    • Journal of Korean Clinical Health Science
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    • v.1 no.1
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    • pp.91-98
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    • 2013
  • Purpose: The purpose is to study the effects of the vision training, as a non-surgically treated vision therapy, on the objective and the subjective symptoms of the patients with intermittent exotropia combined with vertical deviation. Method: For the boys at the age of eight with intermittent exotropia, who report a long-distance blurred vision, dazzling, and intermittent diplopia more than ten times a day, the vision training had been practiced for seven weeks. The objective and subjective symptoms have been examined after six months from the end of the training. Results: The objective and the subjective symptoms have been improved after the seven weeks training and the improvements lasted after six months from the end of the treatment. Conclusion: Non-surgical treatment for the patients with intermittent exotropia, including intermittent exotropia combined with vertical deviation like this case, should be considered as an auxiliary treatment and on the preferential basis.

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Surgical treatment of pulmonary aspergillosis (폐 Aspergillosis 의 외과적 치료)

  • 유회성
    • Journal of Chest Surgery
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    • v.17 no.2
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    • pp.269-274
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    • 1984
  • Since tuberculosis was a common pulmonary disease in Korea, Aspergillosis was easily misdiagnosed as tuberculosis and an acute form of Aspergillosis was misinterpreted as pneumonia because of their similarities in the X-ray findings. This investigation is designed to illustrate the clinical features and preoperative diagnosis and surgical role in the management of this disease. In a retrospective review of operative cases from Jan. 1963 through Dec. 1983, 36 cases were analyzed. Peak age incidence lies in the 3rd decade [41.7%]. All cases had a history of treatment with antituberculous drugs under diagnosis of pulmonary tuberculosis and the most common chief complaint was hemoptysis [69.5%]. Only nine cases [25%] showed cavitary lesions with mycetoma and preoperative sputum study for fungus showed low positive valve [42.3%]. Anatomical location of lesion was located mainly upper lobe [66.7%] and most of cases were managed by lobectomy. We experienced 7 cases of complication; they were postoperative empyema, hepatic failure, esophageal varix bleeding. Postoperative pathologic findings showed that 29 cases [80.5%] were combined with tuberculosis 3 cases were combined with bronchiectasis and 4 cases were not combined with other disease. In conclusion, when the patient has a longstanding history of pulmonary tuberculosis and has a hemoptysis, he must be suspected fungus super infection. Resectional surgery is the treatment of choice for symptomatic localized disease and needed resection in asymptomatic patient to prevent possible fatal sequelae in the future.

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Synergistic Effect of Slightly Acidic Electrolyzed Water and Ultrasound at Mild Heat Temperature in Microbial Reduction and Shelf-Life Extension of Fresh-Cut Bell Pepper

  • Luo, Ke;Oh, Deog-Hwan
    • Journal of Microbiology and Biotechnology
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    • v.25 no.9
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    • pp.1502-1509
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    • 2015
  • The objectives of this study were to evaluate the effect of combined treatments (slightly acidic electrolyzed water (SAEW), ultrasound (US), or mild heat (60℃)) on the growth of Listeria monocytogenes and Salmonella enterica serovar Typhimurium in fresh-cut bell pepper, and the shelf-life and sensory quality (color and texture) were followed during storage at 4℃ and 25℃. An additional 0.65, 1.72, and 2.70 log CFU/g reduction was achieved by heat treatments at 60℃ for 1 min for DW, SAEW, and SAEW+US, respectively. Regardless of the type of pathogen, the combined treatment (SAEW+US+60℃) achieved a significantly (p < 0.05) longer lag time in all treatment groups. This combined treatment also prolonged the shelf-life of bell pepper up to 8 days and 30 h for the storage at 4℃ and 25℃, respectively. There was also no significant difference in the color and hardness of treated (SAEW+US+60℃) bell pepper from that of control during the storage. This new hurdle approach is thus expected to improve the microbial safety of bell peppers during storage and distribution.

Effects of Combined Marine Treatment Program on Risk Factors of Metabolic Syndrome, and CRP in Elderly Musculoskeletal and Metabolic Patients (복합 해양치유 프로그램이 근골격계 및 대사성 질환자의 대사증후군 위험인자 및 CRP에 미치는 영향)

  • Kim, Hyunjun;Shin, Jaesuk
    • Journal of The Korean Society of Integrative Medicine
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    • v.8 no.1
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    • pp.37-45
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    • 2020
  • Purpose : The purpose of this study is to verify the effectiveness of combined marine healing programs by analyzing the physical composition of elderly musculoskeletal and metabolic patients, the risk factors of metabolic syndrome and the effects of the inflammatory factors, the C -reactive protein (CRP). Methods : Individuals with musculoskeletal and metabolic diseases were identified, and marine healing programs were conducted for f our hours each day for two weeks with 11 elderly participants. A one-way RM ANOVA was conducted to determine the differences due to treatment with composite marine healing systems. The results are as follows. Results : After a two-week ocean healing program, weight decreased the most, while BMI also decreased and muscle mass increased. Waist circumference, a risk factor for metabolic syndrome and CRP, decreased, and CRP demonstrated a decreasing trend. Conclusion : The above results show that the two-week marine healing program has a positive effect on the body composition and inflammatory factors of elderly musculoskeletal and metabolic patients.

Effects of Enhanced pCO2 and Temperature on Reproduction and Survival of the Copepod Calanus sinicus

  • Kang, Hyung-Ku;Lee, Chang-Rae;Kim, Dongseon;Yoo, Sinjae
    • Ocean and Polar Research
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    • v.38 no.4
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    • pp.303-314
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    • 2016
  • We tested the combined effects of increased partial pressure of $CO_2$ ($pCO_2$) and temperature on the reproduction and survival of the copepod Calanus sinicus from Asan Bay, the Yellow Sea under laboratory conditions to understand the impact of acidification on copepods. Egg production rate, survival rate, and fecal pellet production of C. sinicus were not affected by 1305 ppm $pCO_2$ or with combined treatments of temperature and $pCO_2$, including $8^{\circ}C$ and 289 ppm $pCO_2$ (ambient), $8^{\circ}C$ and 753 ppm $pCO_2$ (high $pCO_2$), $12^{\circ}C$ and 289 ppm $pCO_2$ (high temperature), and $12^{\circ}C$ and 753 ppm $pCO_2$ (greenhouse), for 5 or 10 d of exposure. However, egg hatching success of C. sinicus decreased significantly in the greenhouse treatment compared with the ambient or the high $pCO_2$ treatments. These results suggest that a combined treatment ($pCO_2$ and temperature) affected egg viability more than a single treatment($pCO_2$).

Effect of Herbicide Combinations on Bt-Maize Rhizobacterial Diversity

  • Valverde, Jose R.;Marin, Silvia;Mellado, Rafael P.
    • Journal of Microbiology and Biotechnology
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    • v.24 no.11
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    • pp.1473-1483
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    • 2014
  • Reports of herbicide resistance events are proliferating worldwide, leading to new cultivation strategies using combinations of pre-emergence and post-emergence herbicides. We analyzed the impact during a one-year cultivation cycle of several herbicide combinations on the rhizobacterial community of glyphosate-tolerant Bt-maize and compared them to those of the untreated or glyphosate-treated soils. Samples were analyzed using pyrosequencing of the V6 hypervariable region of the 16S rRNA gene. The sequences obtained were subjected to taxonomic, taxonomy-independent, and phylogeny-based diversity studies, followed by a statistical analysis using principal components analysis and hierarchical clustering with jackknife statistical validation. The resilience of the microbial communities was analyzed by comparing their relative composition at the end of the cultivation cycle. The bacterial communites from soil subjected to a combined treatment with mesotrione plus s-metolachlor followed by glyphosate were not statistically different from those treated with glyphosate or the untreated ones. The use of acetochlor plus terbuthylazine followed by glyphosate, and the use of aclonifen plus isoxaflutole followed by mesotrione clearly affected the resilience of their corresponding bacterial communities. The treatment with pethoxamid followed by glyphosate resulted in an intermediate effect. The use of glyphosate alone seems to be the less aggressive one for bacterial communities. Should a combined treatment be needed, the combination of mesotrione and s-metolachlor shows the next best final resilience. Our results show the relevance of comparative rhizobacterial community studies when novel combined herbicide treatments are deemed necessary to control weed growth.

A Case Report on Patient with Guillain-Barre Syndrome Improved by Korean Medical Combined Treatment (한방 복합치료로 호전된 Guillain-Barre 증후군 환자 증례)

  • Heo, In;Heo, Kwang-Ho;Hwang, Eui-Hyoung;Shin, Byung-Cheul;Hwang, Man-Suk
    • Journal of Korean Medicine Rehabilitation
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    • v.25 no.1
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    • pp.95-101
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    • 2015
  • Objectives The purpose of this case is to report the improvement after Korean medical combined treatment about patient with Guillain-Barre syndrome. Methods We treated patient using electroacupuncture, moxibustion, herbal medication, and rehabilitation therapy for 59 days. Results The weakness of muscles of upper extremities were recovered to normal level, and the muscle power of lower extremities was improved enough to walking with walker. Conclusions We conclude that Korean medical combined treatment is an effective to improve the power of muscle having weakness. But there is a limit on this report due to sufficient number of case. Further studies will be needed.

Heavy Metals Immobilization in Soil with Plant-growth-promoting Rhizobacteria and Microbial Carbonate Precipitation in Support of Radish Growth

  • He, Jing;Zhang, Qiuzhuo;Achal, Varenyam
    • Microbiology and Biotechnology Letters
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    • v.48 no.2
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    • pp.223-229
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    • 2020
  • The application of plant-growth-promoting rhizobacteria (PGPR) supports the growth of plants in contaminated soil while ureolytic bacteria can immobilise heavy metals by carbonate precipitation. Thus, dual treatment with such bacteria may be beneficial for plant growth and bioremediation in contaminated soil. This study aimed to determine whether the PGPR Pseudomonas fluorescens could work in synergy with ureolytic bacteria to assist with the remediation of cadmium (Cd)- and lead (Pb)-contaminated soils. Pot experiments were conducted to grow radish plants in Cd- and Pb-contaminated soils treated with PGPR P. fluorescens and the results were compared with dual inoculation of P. fluorescens combined with ureolytic Staphylococcus epidermidis HJ2. The removal rate of the metals from the soil was more than 83% for Cd and Pb by the combined treatment compared to 17% by PGPR alone. Further, the dual treatment reduced the metal accumulation in the roots by more than 80%. The translocation factors for Cd and Pb in plant tissues in both treatments remained the same, suggesting that PGPR combined with the carbonate precipitation process does not hamper the transfer of essential metal ions into plant tissues from the soil.