• Environmental Analysis Health and Toxicology
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• v.14 no.1_2
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• pp.1-12
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• 1999

Recently, several new methods for the detection of genetic damages in vitro and in vivo based on molecular biological techniques were introduced according to the rapid progress in toxicology combined with cellular and molecular biology. Among these methods, mouse lymphoma thymidine kanase (tk) gene forward mutation assay, single cell gel electrophoresis (comet assay) and transgenic animal and cell line model as a target gene of lac I (Big Blue) and lac Z (Muta Mouse) gene mutation are newly introduced based on molecular toxicological approaches. The mouse lymphoma tk$\^$+/-/ gene assay (MOLY) using L5178Y tk$\^$+/-/ mouse lymphoma cell line is one of the mammalian forward mutation assays, and has many advantages and more sensitive than hprt assay. The target gene of MOLY is a heterozygous tk$\^$+/-/ gene located in 11 chromosome, so it is able to detect the wide range of genetic changes like point mutation, deletion, rearrangement, and mitotic recombination within tk gene or deletion of entire chromosome 11. The comet assay is a rapid, simple, visual and sensitive technique for measuring and analysing DNA breakages in mammalian cells, Also, transgenic animal and cell line models, which have exogenous DNA incorporated into their genome, carry recoverable shuttle vector containing reporter genes to assess endogenous effects or alteration in specific genes related to disease process, are powerful tools to study the mechanism of mutation in vivo and in vitro, respectively. Also in vivo acridine orange supravital staining micronucleus assay by using mouse peripheral reticulocytes was introduced as an alternative of bone marrow micronucleus assay. In this respect, there was an International workshop on genotoxicity procedure (IWGTP) supported by OECD and EMS (Environmental Mutagen Society) at Washington D. C. in March 25-26, 1999. The objective of IWGTP is to harmonize the testing procedures internationally, and to extend to finalization of OECD guideline, and to the agreement of new guidelines under the International Conference of Harmonization (ICH) for these methods mentioned above. Therefore, we introduce and review the principle, detailed procedure, and application of MOLY, comet assay, transgenic mutagenesis assay and supravital staining micronucleus assay.

• Korean journal of applied entomology
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• v.22 no.2 s.55
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• pp.84-97
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• 1983

Under the limited arable land, the enhancement of agricultural productivity is indispensable to provide the food demand which is concomitant with the rapid increase in population. From this viewpoint, the upbringing and dissemination of high-yielding varieties has been promoted continuously and several modifications in cultural practices, including heavy fertilization, dense planting, and early transplanting, also have been gradually developed. However these changes in cultivation have led to the increased outbreak of insect pests and diseases. And this unexpected results have accelerated the number and complexity of pesticides employed as well as their consumption. Even though pesticides are essential materials contributing to the steady production of agricultural crops, large scale consumption of them has given rise to several adverse impacts, such as mammalian hazard and/or environmental contamination. In this respect, recent development of new pesticides has been concentrated on 'safe pesticide', as it were, that has the highly selective properties without unfavorable side influences on other ecosystem. According to literature cited up to now, feasibilities of safe pesticide development would be summarized as two categories. One of them is the development of chemical pesticides, which include the molecular structure modification of established pesticides for increased safety and synthesis of new safe chemicals which can attack the vulnerable point of physio-ecological characteristics in insect pests and diseases. The other is the biological pesticides which comprise natural enemies and microorganisms to act selectively on confined insect pests and diseases, In addition, improvement of physico-chemical properties of available pesticide formulations would be one of the desirable means for safe pesticide development in view of efficacy enhancement and minimization of hazardous properties or safe pesticide development, various approaches are feasible and needed to study, however, long period and much financial outlay are necessary to develop a new item. And under the present situation in Korea, there are many difficulties for performing research on all the possible routes. Therefore, combined pesticides by the reasonable combination of already registered resticides evaluated as the fairly safe pesticides and safe formulation based on their physico-chemical properties would be developed primarily. And many efforts would be given gradually for the development of new chemical and biological pesticides.

• Transactions of the Korean Society of Automotive Engineers
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• v.18 no.5
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• pp.45-49
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• 2010

The automotive industry has shown a growing interest in tube hydroforming during the past years. The advantages of hydroforming (less thinning, a more efficient manufacturing process, etc.) can, for instance, be combined with the high strength of extra high strength steels, which are usually less formable, to produce structural automotive components which exhibit lower weight and improved service performance. Design and production of tubular components require knowledge about tube material and forming behavior during hydroforming and how the hydroforming operation itself should be controlled. These issues are studied analytically in the present paper. In this study, the whole process of rear sub-frame parts development by tube hydroforming using AA6061 material is presented. At the part design stage, it requires feasibility study and process design aided by CAE (Computer Aided Engineering) to confirm hydroformability in details. Effects of parameters such as internal pressure, axial feeding and geometry shape in automotive rear sub-frame by hydroforming process were carefully investigated. Overall possibility of hydroformable rear sub-frame parts could be examined by cross sectional analyses. Moreover, it is essential to ensure the formability of tube material on every forming step such as pre-bending and hydroforming. In addition, all the components of prototyping tool are designed and interference with press is examined from the point of geometry and thinning.

• Journal of Fashion Business
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• v.18 no.6
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• pp.100-115
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• 2014

This study examined the history of nail art in 20th century, defined characteristics of many techniques used on nails, and analyzed how those techniques have been utilized in recent nail art works. For the method of the study, books and previous studies on nail art were examined as primary sources, and newspaper articles on nail art were studied as references. Then, the case studies were conducted on photographs of nail art works published in Nail Holic in 2012. As a result of analyzing expression techniques of nail art featured on Nail Holic, there had been a total of 517 works created using a single technique. The reason why hand painting is used very frequently is because artists can draw unique images of their own to create various designs according to their tastes. Rhine stone is also very popular, because stones are easy to apply and various in their shapes and colors. There were a total of 555 works created using two or more techniques. The most important point to examine is that hand painting, rhine stone, and 3D art were most popular among all techniques. These works featured highly various effects and images by combining flat effect of hand painting with three-dimensional effects of rhine stone or 3D art. The tendency can also be found in the fact that there have been many works using techniques of flat effects such as decal or marbling combined with techniques of three-dimensional effects such as rhine stone or 3D art.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.47 no.3
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• pp.248-256
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• 2011

The inherent efficiency of a ship would be prior to any other quality factors in ship's safety, because lack of it may give rise to a serious sea casuality even if it were a light mistake in operation. And the representative casualty comes from a deficiency of stability and an operating error combined would be capsizing. The Korean offshore large purseiner looks to have a structural weak point with small range of stability in spite of her big initial metacentric height, and have various type of roll reduction devices in order to cover up the defect. The aim of this study is to grasp for the roll reduction characteristics of the purseiner with bilge keel only and a stern keel additionally attached. The results are summarized as follows; The rolling angle of the model ship was increased in accordance with increase of the wave period and height, especially at close to the natural wave period of her, and the trends were more distinguished in the situation of bilge keel only installation than in the stern keel additionally installed. And stern keel has not noticeable effect on the reduction of the roll in the light ship condition, but has a little effect in full load condition.

• Tunnel and Underground Space
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• v.22 no.3
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• pp.221-225
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• 2012

Magnesium is one of the light weight materials, which can improve fuel economy and reduce emissions in automotive industry. Recently, magnesium alloys have gained considerable attention due to good mechanical properties. In this work, we have performed an explosive welding using the magnesium alloys (AZ31) and stainless steel (SUS 304). As a result, SUS304/AZ31 were successfully combined each other; however, a resolidified interlayer was observed at the point of welded layer. To reduce the resolidified interlayer, we have changed the thickness (0.5 mm and 1 mm) of stainless steel, distance (45 mm and 60 mm) between explosive and the center of materials and initial angle ($20^{\circ}$ and $30^{\circ}$) of explosive. In the case of the thickness 0.5 mm and angle of $30^{\circ}$, the resolidfied interlayer was not observed due to the increase of distance from the explosive. To accurately estimate the resolidified interlayer, electron probe micro-analyzer (EPMA) method and hardness were used. For the EPMA analysis, mixed materials were confirmed at the resolidified interlayer, and the measurement exhibited the middle value compared with the AZ31 and SUS304.

• Korean Journal of Applied Biomechanics
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• v.15 no.2
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• pp.41-55
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• 2005

In this study, weight carrying pattern analysis and comparison method of four foot region were suggested. We used three types of club(driver, iron7, pitching wedge). This analysis method can compare between top class golfer and beginner. And the comparison data can be used to correct the swing pose of trainee. If motion analysis system, which can measure the swing speed and instantaneous acceleration at the point of hitting a ball, is combined with this plantar foot force analysis method, new design development of golf shoes to increase comfort and ball flight distance will be available. 1. Regional change of force acting, in address, is evenly distributed on both feet. In back swing top, 76% on right foot, 75% on left foot as impact, and 86% on left foot as finish. As regional force acting, in address, pros get high marks on rare and inside of right foot and rare and outside for amateurs. In back swing top, it is higher as fore and inside of left foot, pros as rare part of right foot and amateurs as forefoot. In impact, it is higher for pros and amateurs in outside and rare part of left foot and fore and inside of right foot. In finish, for both pros and amateurs, it is higher for outside and rare parts of left foot. 2. For each club, forces are evenly distributed on both feet in address. In back swing top, the shorter a club is, the higher impact on right foot and the higher finish distribution on left foot. For all the clubs used, in each region, pros get higher on rare and inside of right foot and as amateurs on rare and outside of left foot in address. In back swing top, for all clubs, pros get higher on rare and outside of right foot as fore and outside for amateurs. In impact acting, for all clubs, rare and outside of left foot get higher. In finish, force concentrates on rarefoot. 3. On both feet force, right foot forces of amateurs is higher than those of pros in back swing top. In impact and finish, pros get higher on left foot.

• Proceedings of the KSRS Conference
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• 2005.10a
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• pp.182-185
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• 2005

Satellite cameras are calibrated before launch in detail and in general, but it cannot be guaranteed that the geometry is not changing during launch and caused by thermal influence of the sun in the orbit. Modem satellite imaging systems are based on CCD-line sensors. Because of the required high sampling rate the length of used CCD-lines is limited. For reaching a sufficient swath width, some CCD-lines are combined to a longer virtual CCD-line. The images generated by the individual CCD-lines do overlap slightly and so they can be shifted in x- and y-direction in relation to a chosen reference image just based on tie points. For the alignment and difference in scale, control points are required. The resulting virtual image has only negligible errors in areas with very large difference in height caused by the difference in the location of the projection centers. Color images can be related to the joint panchromatic scenes just based on tie points. Pan-sharpened images may show only small color shifts in very mountainous areas and for moving objects. The direct sensor orientation has to be calibrated based on control points. Discrepancies in horizontal shift can only be separated from attitude discrepancies with a good three-dimensional control point distribution. For such a calibration a program based on geometric reconstruction of the sensor orientation is required. The approximations by 3D-affine transformation or direct linear transformation (DL n cannot be used. These methods do have also disadvantages for standard sensor orientation. The image orientation by geometric reconstruction can be improved by self calibration with additional parameters for the analysis and compensation of remaining systematic effects for example caused by a not linear CCD-line. The determined sensor geometry can be used for the generation? of rational polynomial coefficients, describing the sensor geometry by relations of polynomials of the ground coordinates X, Y and Z.

• Journal of Korean Society of Coastal and Ocean Engineers
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• v.23 no.1
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• pp.70-78
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• 2011

At Haeundae Beach in Busan, people were swept away by the fast-moving rip current and rescued, on August 13th and 15th, 2009 & July 29th and 30th, 2010. In predicting the wave-induced current and consequent tube movement for public safety, the coastal flows and waves are calculated at each time step and grid point by means of systematic interfacing of hydrodynamic and wave models (Lee, 2001). To provide a user-friendly simulation tool for end-users, the forecasting system has been built in a software package called HAECUM. Outputs from the system are viewed as graphs of tube positions with combined current vectors for easy decision of emergency management officials. The tube-wave interaction is taken into account and the traces of swim tube are simulated by using a Lagrangian random walk (Chorin, 1978; Lee, 1994). In this study, we use the Lee's approach (Lee, 1993) in estimating the surface onshore currents due to wave breaking.

• Journal of Electrical Engineering and Technology
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• v.6 no.6
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• pp.806-816
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• 2011

Interest about social security has recently increased in favor of safety for infrastructure. In addition, advances in computer vision and pattern recognition research are leading to video-based surveillance systems with improved scene analysis capabilities. However, such video surveillance systems, which are controlled by human operators, cannot actively cope with dynamic and anomalous events, such as having an invader in the corporate, commercial, or public sectors. For this reason, intelligent surveillance systems are increasingly needed to provide active social security services. In this study, we propose a core technique for intelligent surveillance system that is based on swarm robot technology. We present techniques for invader enclosing using swarm robots based on multiple distributed object environment. The proposed methods are composed of three main stages: location estimation of the object, specified object tracking, and decision of the cooperative behavior of the swarm robots. By using particle filter, object tracking and location estimation procedures are performed and a specified enclosing point for the swarm robots is located on the interactive positions in their coordinate system. Furthermore, the cooperative behaviors of the swarm robots are determined via the result of path navigation based on the combination of potential field and wall-following methods. The results of each stage are combined into the swarm robot-based invader-enclosing technique on multiple distributed object environments. Finally, several simulation results are provided to further discuss and verify the accuracy and effectiveness of the proposed techniques.