Mucin2 (MUC2), an important regulatory factor in the immune system, plays an important role in the host defense system against bacterial translocation. Probiotics known to regulate MUC2 gene expression have been widely studied, but the interactions among probiotic, pathogens, and mucin gene are still not fully understood. The aim of this study was to investigate the role of MUC2 in blocking effects of probiotics on meningitic E. coli-induced pathogenicities. In this study, live combined probiotic tablets containing living Bifidobacterium, Lactobacillus bulgaricus, and Streptococcus thermophilus were used. MUC2 expression was knocked down in Caco-2 cells by RNA interference. 5-Aza-2'-deoxycytidine (5-Aza-CdR), which enhances mucin-promoted probiotic effects through inducing production of Sadenosyl-L-methionine (SAMe), was used to up-regulate MUC2 expression in Caco-2 cells. The adhesion to and invasion of meningitic E. coli were detected by competition assays. Our studies showed that probiotic agents could block E. coli-caused intestinal colonization, bacteremia, and meningitis in a neonatal sepsis and meningitis rat model. MUC2 gene expression in the neonatal rats given probiotic agents was obviously higher than that of the infected and uninfected control groups without probiotic treatment. The prohibitive effects of probiotic agents on MUC2-knockdown Caco-2 cells infected with E44 were significantly reduced compared with nontransfected Caco-2 cells. Moreover, the results also showed that 5-Aza-CdR, a drug enhancing the production of SAMe that is a protective agent of probiotics, was able to significantly suppress adhesion and invasion of E44 to Caco-2 cells by upregulation of MUC2 expression. Taken together, our data suggest that probiotic agents can efficiently block meningitic E. coli-induced pathogenicities in a manner dependent on MUC2.
Journal of Korean Society of Environmental Engineers
/
v.31
no.11
/
pp.1007-1018
/
2009
Hydrocyclone is widely used in industry, because of its simplicity in design, high capacity, low maintenance and operational cost. The separation action of a hydrocyclone treating particulate slurry is a consequence of the swirling flow that produces a centrifugal force on the fluid and suspended particles. In spite of hydrocyclone have many advantage, the application for treatment of urban stormwater case study were rare. We conducted a laboratory scale study on treatable potential of micro particles using hydrocyclone filter (HCF) that was a combined modified hydrocyclone with perlite filter cartridge. Since it was not easy to use actual storm water in the scaled-down hydraulic model investigations, it was necessary to reproduce ranges of particles sizes with synthetic materials. The synthesized storm runoff was made with water and addition of particles; ion exchange resin, road sediment, commercial area manhole sediment, and silica gel particles. Experimental studies have been carried out about the particle separation performance of HCF-open system and HCF-closed system. The principal structural differences of these HCFs are underflow zone structure and vortex finder. HCF was made of acryl resin with 120 mm of diameter hydrocyclone and 250 mm of diameter filter chamber and overall height of 800 mm. To determine the removal efficiency for various influent concentrations of suspended solids (SS) and chemical oxygen demand (COD), tests were performed with different operational conditions. The operated maximum of surface loading rate was about 700 $m^3/m^2$/day for HCF-open system, and 1,200 $m^3/m^2$/day for HCF-closed system. It was found that particle removal efficiency for the HCF-closed system is better than the HCF-open system under same surface loading rate. Results showed that SS removal efficiency with the HCF-closed system improved by about 8~20% compared with HCF-open system. The average removal efficiency difference for HCF-closed system between measurement and CFD particle tracking simulation was about 4%.
Asia-Pacific Journal of Business Venturing and Entrepreneurship
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v.15
no.2
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pp.19-32
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2020
The purpose of this study was to investigate the influence factors of the university level centering on the entrepreneurial performance of the university students and full-time faculties in the situation of increasing interest in entrepreneurial university. In order to achieve the purpose of the study, a panel data was established from 2015 to 2018 on the basis of the university notification data. The panel data included universities with data on the number of university students and full-time faculty founders for at least two years. Through this, four year data from 154 universities were used for analysis. As an analysis method, frequency analysis and descriptive statistics were conducted to understand the characteristics of the university. Since then, panel negative binomial regression analysis has been conducted in consideration of the longitudinal features and distribution of the data. Also, based on the Hausman test results, the results were interpreted based on random effect model. The results of this study are as follows. First, as a result of the analysis of the entrepreneurial performance and the change trend of the domestic university from 2015 to 2018, the entrepreneurial performance of the university has been steadily increasing in the last four years, and the increase in the number of university student entrepreneurs was relatively higher than the full-time faculties. Second, economic and educational approaches need to be combined to promote university students' start-ups. The university factors that promote the start-up of university students were found to be scholarships, start-up grants, startup lectures, and startup clubs. Third, the openness and regional characteristics of the univeristy can promote the establishment of university students. Fourth, the establishment of a research environment and support for start-ups for full-time faculty members can enhance their start-up performance. The university factors that promote the start-up of full-time faculty were research funds and staffes who support start-up. The conclusions drawn from these findings are as follows. First, overall efforts are needed to develop into an entrepreneurial university. Second, in order to change into an entrepreneurial university, direct support for entrepreneurship is needed. Third, as an entrepreneurial university, it is necessary to find a way to bridge the gap by university according to region and size. Fourth, it is necessary to reinforce the support for linking the research results of universities to start-ups. Fifth, it is necessary to improve the atmosphere for full-time faculty members to be entrepreneur.
Journal of Korean Home Economics Education Association
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v.24
no.3
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pp.225-239
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2012
The purpose of this study was to develope and implement a teaching learning process plan applied with portfolio assessment for 'housing and residential environment' section of Technology Home Economics in a middle school. The teaching learning process plan consisting of 8-session lessons had been developed and implemented according to the ADDIE model mixed with 6 portfolio-development stages. In the development stage, 19 activity materials(5 reading texts, 6 individual and 2 group activity sheets, and 3 subject activity sheets) and 27 teaching learning materials(8 sets of pictures & photos and 19 moving pictures) were developed for the 8-session lessons. The plans applied to 2 classes 74 students in the third grade of K middle school in Gyeonggi-do during May 16th-17th of June, 2011. The results from survey and portfolio showed that the 8-session lessons had overall achieved the general goal of the teaching learning process plan applied with portfolio assessment, which was to stimulate students in the class through working with portfolio elements of activity materials. The students evaluated the whole process of 8 lessons were adequate and helpful. Students also reported they highly accomplished the goal of each lesson and actively participated in the lesson. The 3 subject activity sheets as well as other materials in the portfolio were excellently done with the average of over 90% points. These results supported that a teaching learning process plan applied with portfolio assessment was a combined lesson with evaluation and also an alternative to qualitative evaluation over the whole units. This plan might apply to other parts of housing as well as various other areas.
Kim, Seong Hee;Lee, Woo Chun;Cho, Hyen Goo;Kim, Soon-Oh
Journal of the Mineralogical Society of Korea
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v.25
no.4
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pp.197-210
/
2012
Hematite has been known to be the most stable form of various iron (oxyhydr)oxides in the surface environments. In this study, its properties as an adsorbent were examined and also adsorption of arsenic onto hematite was characterized as well. The specific surface area of hematite synthesized in our laboratory appeared to be $31.8g\;m^2/g$ and its point of zero salt effect, (PZSE) determined by potentiometric titration was observed 8.5. These features of hematite may contribute to high capacity of arsenic adsorption. From several adsorption experiments undertaken at the identical solution concentrations over pH 2~12, the adsorption of As(III) (arsenite) was greater than that of As(V) (arsenate). As of pH-dependent adsorption patterns, in addition, arsenite adsorption gradually increased until pH 9.2 and then sharply decreased with pH, whereas adsorption of arsenate was greatest at pH 2.0 and steadily decreased with the increasing pH from 2 to 12. The characteristics of these pH-dependent adsorption patterns might be caused by combined effects of the variation in the chemical speciation of arsenic and the surface charge of hematite. The experimental results on adsorption kinetics show that adsorption of both arsenic species onto hematite approached equilibrium within 20 h. Additionally, the pseudo-second-order model was evaluated to be the best fit for the adsorption kinetics of arsenic onto hematite, regardless of arsenic species, and the rate constant of As(V) adsorption was investigated to be larger than that of As(III).
The aim of this study was to investigate whether fourier transform infrared (FT-IR) spectroscopy can be applied to simultaneous determination of fatty acids contents in different soybean cultivars. Total 153 lines of soybean (Glycine max Merrill) were examined by FT-IR spectroscopy. Quantification of fatty acids from the soybean lines was confirmed by quantitative gas chromatography (GC) analysis. The quantitative spectral variation among different soybean lines was observed in the amide bond region ($1,700{\sim}1,500cm^{-1}$), phosphodiester groups ($1,500{\sim}1,300cm^{-1}$) and sugar region ($1,200{\sim}1,000cm^{-1}$) of FT-IR spectra. The quantitative prediction modeling of 5 individual fatty acids contents (palmitic acid, stearic acid, oleic acid, linoleic acid, linolenic acid) from soybean lines were established using partial least square regression algorithm from FT-IR spectra. In cross validation, there were high correlations ($R^2{\geq}0.97$) between predicted content of 5 individual fatty acids by PLS regression modeling from FT-IR spectra and measured content by GC. In external validation, palmitic acid ($R^2=0.8002$), oleic acid ($R^2=0.8909$) and linoleic acid ($R^2=0.815$) were predicted with good accuracy, while prediction for stearic acid ($R^2=0.4598$), linolenic acid ($R^2=0.6868$) had relatively lower accuracy. These results clearly show that FT-IR spectra combined with multivariate analysis can be used to accurately predict fatty acids contents in soybean lines. Therefore, we suggest that the PLS prediction system for fatty acid contents using FT-IR analysis could be applied as a rapid and high throughput screening tool for the breeding for modified Fatty acid composition in soybean and contribute to accelerating the conventional breeding.
Purpose : Ginkgo biloba extract(GBE) is known to increase the peripheral blood circulation. This study was designed to evaluate the effect of GBE on the acute normal tissue radiation reaction. Materials and Methods : mice were divided into two groups, radiation alone and two doses GBE plus radiation, for both acute skin reaction and jejunal crypt assay. GBE was given i.p. one hour before irradiation with priming dose given one day earlier. Thirty to Fifty Gy for acute skin reaction and 11 to 14 Gy for jejunal crypt were irradiated to right hind leg and whole body, respectively. Results : Radiation doses($RD_{50}$) for Peak skin score of 2.0 were 44.2Gy (40.6-48.2Gy) for radiation alone and 44.4Gy(41.6-47.4Gy) for two doses GBE plus radiation, showing no effect of GBE on acute radiation skin damage. The numbers of regenerating jejunal crypts per circumference were also almost the same for each radiation dose level(p=0.57-0.94), and the mean lethal doses($D_o$) were 1.800y(1.57-2.09Gy) for radiation alone and 1.88Gy(1.65-2.18Gy) for two doses GBE plus radiation, indicating no effect of GBE on jejunal crypt cell survival after radiation. Conclusion : GBE doesn't increase acute normal tissue radiation reaction in this model system. As GBE was verified to enhance radiation effect on tumor, high therapeutic gain is expected when GBE is combined with radiation therapy.
Germination of Codonopsis pilosula seeds and afterward their seedling emergence have been occasionally very poor due to their dormancy and structural defects. The study was done to determine the treatment effects of priming, $GA_3$, agitation and washing during and after priming, drying and water imbibition after drying on germination of its seeds and then of the successive three combined treatment after priming on the its seedling emergence to model its presown seed treatment. While agitation at the speed of 10 rpm was made or not, priming using $Ca(NO_3)_2$ and $GA_3$ under their different concentrations and light quality illuminated for 12 hours a day were done separately. Then their two best results were compared to determine the better one. Using the above determined best result, washing was done 3 minutes in the tap water or not, and drying of washed seeds was made under 4 different lights, and water imbibition of the dried seeds with differed period were successively done to deduce its presown seed treatment. In the comparison of the best germination rate of priming and $GA_3$, the former showed considerably higher rate than the latter; the priming treatment was done at agitated $Ca(NO_3)_2$ 150 mM solution under darkness or 12 hour red light illumination a day for 2 days. Washing after priming enhanced the rate compared to no-washing. Drying was the best way at which seeds washed were desiccated under $35^{\circ}C$ and 4 hour red light illumination. Water imbibition immediately before sowing must be made for 2 days. Seedling emergence rate was the highest at the seeds from successive 4 treatments, priming, washing, drying and water imbibition before sowing of two others, the priming and washing, or the priming, washing and water imbibition, meaning that its presown seed treatment must follow the procedure of the above 4 successive ones.
The Sr and Nd isotopic compositions of two foliated granitic plutons located in the Chonju and Sunchang area were determined in order to reconfirm the intrusion ages of granitoids and to study the sources of granitic magmas. The best defined Rb-Sr isochron for the whole rock samples of the Chonju foliated granite (CFGR) give an age of $284{\pm}12Ma$, suggesting early Permian intrusion age. In contrast, the whole rock Rb-Sr data of the Sunchang foliated granite (SFGR) scatter widely on the isochron diagram with very little variation in the $^{87}Rb/^{86}Sr$ ratios and, therefore, yield no reliable age information. Futhermore they show the concordance of mineral and whole rock Rb-Sr isochron and divide into two linear groups with roughly the same slopes and significantly different $^{87}Sr/^{86}Sr$ ratios, indicating some kind of Rb-Sr distortion in whole rock scale and a difference in source material and/or magmatic evolution between two subsets. The reconstructed isochrons of 243 Ma, which was defined from the proposed data by the omission of one sample point with significantly higher $^{87}Rb/^{86}Sr$ ratio than the others, and 252 Ma, from the combined data of it and some of this study, strongly suggest the possibility that the SFGR was intruded appreciably earlier than had previously been proposed, although the reliability of these ages still questionable owing to high scatter of data points and, therefore, further study is necessary. All mineral isochrons for the investigated granites show the Jurassic to early Cretaceous thermal episode ranging from 160 Ma to 120 Ma Their corresponding initial $^{87}Sr/^{86}Sr$ ratios correlate well with their whole rock data, indicating that the mineral Rb-Sr system of the investigated granites was redistributed by the postmagmatic thermal event during Jurassic to early Cretaceous. The initial ${\varepsilon}Sr$ values for the CFGR (64.27 to 94.81) tend to be significantly lower than those for the SFGR (125.43 to 167.09). Thus it is likely that there is a marked difference in the magma source characteristics between the CFGR and the SFGR, although the possibility of an isotopic resetting event giving rise to a high apparent initial ${\varepsilon}Sr$ in the SFGR can not be ruled out. In contrast to ${\varepsilon}Sr$, both batholiths show a highly resticted and negative values of initial ${\varepsilon}Nd$, which is -14.73 to -19.53 with an average $-16.13{\pm}1.47$ in the CFGR and -14.78 to -18.59 with an average $-17.17{\pm}1.01$ in the SFGR. The highly negative initial ${\varepsilon}Nd$ values in the investigated granitoids strongly suggest that large amounts of recycled old continental components have taken part in their evolution. Furthermore, this highly resticted variation in ${\varepsilon}Nd$ is significant because it requires that the old crustal source material, from which the granitoid-producing melts were generated, should have a reasonably uniform Nd isotopic composition and also quit similar age. Calculated T2DM model ages give an average of $1.83{\pm}0.25Ga$ for CFGR and $1.96{\pm}0.19Ga$ for SFGR, suggesting the importance of a mid-Proterozoic episode for the genesis of two foliated granites. Although it is not possible to determine precisely the source rock compositions for the investigated foliatic granites, the Sr-Nd isotopic evidences indicate that midcrustal or less probably, a lower crustal granulitic source could be the most likely candidate.
The Okcheon Group in the southwestern part of the Okcheon Metamorphic Belt is subdivided into two distinct tectonostratigraphic units: the Boeun unit in the south and the Pibanryeong unit in the north. The Boeun unit consists of petites, psammites, carbonaceous petites, limestones and pebble-bearing quartzites. The Pibanryeong unit is composed of petites, well-sorted fine-grained psammites, carbonaceous psammites and quartzites. In order to outlining stratigraphy and depositional environments of the Okcheon Group, detailed stratigraphic sections were measured in three locations; one section(Gosan section) of the Boeun unit and two sections(Sorungjae and Hwangryeongzae sections) of the Pibanryeong unit. The Gosan section of the Boeun unit is interpreted to be deposited in the shallow marine environments, whereas the Sorungjae and Hwangryeonaiae sections of the Pibanryeong unit appear to be deposited in slope and deep basin environments. This result indicates rapid subsidence between deposition of the Boeun and Pibanryeong units in sedimentary environment. The trace of sedimentological environments in the Hwasan area was investigated by geochemical analysis of 109 metapelitic and psammitic rock samples. Distinct chemical variations of politic and psammitic rocks from the Boeun and Pibanryeong units in the study area are evident from plots of major elements and $A1_2O_3$/$SiO_2$ versus Basicity Index($Fe_2O_3{+}MgO$)/($SiO_2{+}K_2O{+}Na_2O$). The rocks show a progressive chemical trend from the Boeun unit to the Pibanryeong unit on these diagrams. They in the southern sector of the Boeun unit display lower values and a comparatively wide range of $A1_2O_3$/$SiO_2$ and Basicity Index, as compared with those from the northern sector of the Boeun and Pibanryeong units. The southern sector of the Pibanryeong unit including narrow staurolite-bearing zone is characterized by values that are transitional between the Boeun and Pibanryeong units. These data, combined with depositional environment progressively deepened towards the northwest, support a half-graben model for the Okcheon basin, as proposed by Cluzel et al.(1990)
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