• Bulletin of the Korean Chemical Society
• /
• 제21권9호
• /
• pp.919-922
• /
• 2000

The cyclization reaction of N-(2-hydroxyethyl)-N'-phenylthioureas 2 containingambident nucleophile was ex-amined in the combination of a variety ofbases and p-toluenesulfonyl chloride (TsCl).N-(2-Hydroxyeth-yl) thioureas 2 were readily obtained in high yields from the reaction of the corresponding 1,2-aminoalcohols with phenyl isothiocyanate, avoiding the need for O-protection.The use of a one-pot reaction (NaOH/TsCl) was found to be most effective in producing the requisite 2-phenylaminothiazolines (S-cyclization) 3 in the case ofthioureas 2a-2e derived from N-unsubstituted aminoalcohols,while in the thioureas 2f and 2g prepared from N-substituted aminoalcohols the combination of Et3N and TsCl led to the S-cyclization products.

• 한국측량학회:학술대회논문집
• /
• 한국측량학회 2006년도 춘계학술발표회 논문집
• /
• pp.99-104
• /
• 2006

The new gravity field combination models are expected to improve the knowledge of the Earth's global gravity field. This study evaluates eleven global gravity field models derived from gravimetry and altimetry surface data in a comparison with ground truth in South Korea. Geoid heights obtained from GPS and levelling in South Korea are compared with geoid heights from the models. The results show that the gravity satellites CHAMP, GRACE and LAGEOS plus gravimetry and altimetry surface data have led to an improvement in gravity field models. As expected, the new combination gravity field model which are EIGEN-CG03C and EIGEN-GL04C give better results than the predecessors widely used models(EGM96, OSU91A etc.).

• 한국어병학회지
• /
• 제35권1호
• /
• pp.113-120
• /
• 2022

Scuticociliatosis caused by Miamiensis avidus is a very important parasitic disease in olive flounder farming industry. The aim of this study was to determine effect of combined treatment with blue LED (light-emitting diode) illumination and formalin on olive flounder (Paralichthys olivaceus) infected with M. avidus. Different intensity of 405 nm LED (20, 40, and 60 μmol·m^-2·s^-1) was illuminated on 2.2×10⁴ cells/well of M. avidus in a 24 well microplate for 24 h. Also, 2.4×10⁴ cells/well of M. avidus were exposed to varying combinations of 60 μmol·m^-2·s^-1 of 405 nm LED and serial 10-fold dilutions of formalin (from 10 to 100 ppm) for 15, 30, 45, and 60 min. Surviving M. avidus were counted using a hemocytometer. For in vivo test, flounder acclimatized at 11-12 practical salinity unit (psu) were challenged with 2×10⁶ cells/ml of M. avidus by immersion method for 1 h. Then, fish were moved and divided into four groups; "F" group, treated with formalin at 50 ppm; "L" group, treated with 60 μmol·m^-2·s^-1 of 405 nm LED; "C" group, treated with combination of the two methods; and the control group. After treatment for 30 min, fish were transferred to new tanks (salinity = 11-12 psu) and observed for 3 weeks. As a result, illumination of 405 nm LED at 60 μmol·m^-2·s^-1 killed 100% of M. avidus after 12 h, while 67% and 90% of the scuticociliate died at 20 and 40 μmol·m^-2·s^-1, respectively, after 24 h exposure. One hundred percent of M. avidus was killed at 90, 80, 80 and 70 ppm after exposure to formalin for 15, 30, 45 and 60 min, respectively. However, combined method (e.g., 60 μmol·m^-2·s^-1 of 405 nm-LED plus 50 ppm formalin) killed the parasite within 30 min. From in vivo test, similarly, survival rates of fish challenged with M. avidus were 100%, 43%, 29% and 0% in the C, F, L, and control groups, respectively. Results obtained in this study demonstrates that the combined treatment method has clear synergistic effect on scuticociliatosis in fish.

• Journal of Microbiology and Biotechnology
• /
• 제26권8호
• /
• pp.1490-1503
• /
• 2016

Colorectal cancer (CRC) is the third most common cancer in the world. Although 5-fluorouracil (5-FU) is the representative chemotherapy drug for colorectal cancer, it has therapeutic limits due to its chemoresistant characteristics. Colorectal cancer cells can develop into cancer stem cells (CSCs) with self-renewal potential, thereby causing malignant tumors. The human gastrointestinal tract contains a complex gut microbiota that is essential for the host's homeostasis. Recently, many studies have reported correlations between gut flora and the onset, progression, and treatment of CRC. The present study confirms that the most representative symbiotic bacteria in humans, Lactobacillus plantarum (LP) supernatant (SN), selectively inhibit the characteristics of 5-FU-resistant colorectal cancer cells (HT-29 and HCT-116). LP SN inhibited the expression of the specific markers CD44, 133, 166, and ALDH1 of CSCs. The combination therapy of LP SN and 5-FU inhibited the survival of CRCs and led to cell death by inducing caspase-3 activity. The combination therapy of LP SN and 5-FU induced an anticancer mechanism by inactivating the Wnt/β-catenin signaling of chemoresistant CRC cells, and reducing the formation and size of colonospheres. In conclusion, our results show that LP SN can enhance the therapeutic effect of 5-FU for colon cancer, and reduce colorectal cancer stem-like cells by reversing the development of resistance to anticancer drugs. This implies that probiotic substances may be useful therapeutic alternatives as biotherapeutics for chemoresistant CRC.

• Parasites, Hosts and Diseases
• /
• 제61권3호
• /
• pp.251-262
• /
• 2023

Schistosomiasis causes significant morbidity and mortality worldwide. This study aimed to assess the effect of schistosomula lung antigen preparation (SLAP) and soluble egg antigen (SEA) on a murine schistosomiasis mansoni model. Ninety laboratory-bred male Swiss albino mice were divided into 6 groups. Two doses of the vaccine were given at 2-week intervals. All mice were subcutaneously infected with 80±10 Schistosoma mansoni cercariae 2 weeks after the last vaccination dose. They were sacrificed 7 weeks post-infection. Parasitological and histopathological studies were conducted to assess the effect of inoculated antigens (single or combined). The results showed that the combination of SLAP and SEA (combination group) led to a significant reduction in worm burden (65.56%), and liver and intestine egg count (59% and 60.59%, respectively). The oogram pattern revealed a reduction in immature and mature eggs (15±0.4 and 10±0.8, respectively) and an increased number of dead eggs in the combination group (P<0.001). In terms of histopathological changes, the combination group showed notably small compact fibrocellular egg granuloma and moderate fibrosis in the liver. A high percentage of destroyed ova was observed in the intestine of the combination group. This study demonstrates for the first time the prophylactic effect of combined SLAP and SEA vaccine. The vaccine induced a significant reduction in the parasitological and pathological impacts of schistosomiasis mansoni in hepatic and intestinal tissues, making it a promising vaccine candidate for controlling schistosomiasis.

• Journal of Microbiology and Biotechnology
• /
• 제21권12호
• /
• pp.1306-1311
• /
• 2011

Recombinant Rhodopseudomonas palustris, harboring the carotenoid-metabolizing gene crtI (CrtIBS), and whose color changes from greenish yellow to red in response to inorganic As(III), was cultured in transparent microplate wells illuminated with a light emitting diode (LED) array. The cells were seen to grow better under near-infrared light, when compared with cells illuminated with blue or green LEDs. The absorbance ratio of 525 to 425 nm after cultivation for 24 h, which reflects red carotenoid accumulation, increased with an increase in As(III) concentrations. The detection limit of cultures illuminated with near-infrared LED was 5 ${\mu}g$/l, which was equivalent to that of cultures in test tubes illuminated with an incandescent lamp. A near-infrared LED array, in combination with a microplate, enabled the simultaneous handling of multiple cultures, including CrtIBS and a control strain, for normalization by the illumination of those with equal photon flux densities. Thus, the introduction of a near-infrared LED array to the assay is advantageous for the monitoring of arsenic in natural water samples that may contain a number of unknown factors and, therefore, need normalization of the reporter event.

• The Journal of Advanced Prosthodontics
• /
• 제10권4호
• /
• pp.291-299
• /
• 2018

PURPOSE. To assess the degree of conversion (DC) and light irradiance delivered to light-cured and dual-cured cements by application of different light sources through various types of monolithic computer-aided design and computer-aided manufacturing (CAD/CAM) materials. MATERIALS AND METHODS. RelyX Ultimate Clicker light-cured and dual-cured resin cement specimens with 1.5-mm thicknesses (n=300, 10/group), were placed under four types of crystalline core structure (Vita Enamic, Vita Suprinity, GC Ceresmart, Degudent Prettau Anterior). The specimens were irradiated for 40 seconds with an LED Soft-Start or pulse-delay unit or 20 seconds with a QTH unit. DC ratios were determined by using Fourier transform infrared spectroscopy (FTIR) after curing the specimen at 1 day and 1 month. The data were analyzed using the Mann-Whitney U test (for paired comparison) and the Kruskal-Wallis H test (for multiple comparison), with a significance level of P<.05. RESULTS. DC values were the highest for RelyX Ultimate Clicker light-cure specimens polymerized with the LED Soft-Start unit. The combination of the Vita Suprinity disc and RelyX Ultimate Clicker dual-cure resin cement yielded significantly higher values at both timepoints with all light units (all, P<.05). CONCLUSION. Within the limitations of this study, we conclude that the DC of RelyX Ultimate Clicker dual-cure resin cement was improved significantly by the use of Vita Suprinity and the LED Soft-Start light unit. We strongly recommend the combined use of an LED light unit and dual-cure luting cement for monolithic ceramic restorations.

• 한국인터넷방송통신학회논문지
• /
• 제19권4호
• /
• pp.47-54
• /
• 2019

전세계적으로 기후 변화의 영향에 따른 차량 추돌 사고에 대한 위험성이 중요한 이슈로 등장하고 있다. 고속주행차량의 속도제한을 위해 다양한 형태의 속도제한 표시장치가 고려되고 있으나 현재는 대부분 고정식 속도제한 표시기를 사용하고 있다. 4계절이 뚜렷한 우리나라의 경우에는 비가 많이 오는 우기나 눈이 많이 오는 계절이나 안개가 많이 발생되는 경우에는 차량 추돌 사고를 방지하기 위한 차량 속도 감속 제한이 필요하지만 기존에 설치되어 있는 고정식 표지판으로는 사고를 미연에 방지하는 것이 어려운 실정이다. 본 연구에서는 레인 센서와 차량 와이퍼 시스템 등을 결합 시킨 기후 변화에 따른 차량 속도 제한이 가변적으로 표시되는 LED 전광판의 프로토타입 모델을 제작하였다. 내리는 비나 눈의 양에 따라 기준속도의 100%, 80%, 50%로 3단계로 가변 표시되는 속도제한 표시기는 설치되는 장소에 따라 적절한 속도를 선택 지정하여 표시할 수 있다. LED 표시장치를 이용한 시각적인 효과로 인해 운전자들이 사전에 속도를 감속하게 하여 차량 추돌 사고를 미연에 방지하는데 효과적으로 이용될 것으로 기대 된다.

• 한국콘텐츠학회논문지
• /
• 제15권9호
• /
• pp.96-105
• /
• 2015

최근 백화점에서는 고객 유치와 홍보적 수단으로 미디어파사드를 활용하고 있다. 특히, 대형 백화점을 이용하는 고객을 살펴보면 남성보다 여성의 비율이 4.6배나 많다. 이는 성별에 따른 차별화된 미디어파사드 콘텐츠를 제작해야 하는 필요성이 제기되고 있다. 이에 본 연구는 남녀에 의한 미디어파사드의 인지정도에 따른 실증분석을 목적으로 하고자 한다. 먼저 선행연구를 통해 심미성, 예술성, 쾌적성, 조화성 총4개의 인지요인을 도출하고 "각 인지요인이 성별에 따라 지각하는 데는 차이가 있을 것이다"라는 가설을 설정하였다. 이를 검정하기 위해 미디어파사드가 설치되어 있는 국내 대형 백화점을 선정하고 실증실험을 수행하였다. 실험결과, 심미성, 예술성, 조화성은 채택되었고 쾌적성은 기각되었다. 이는 백화점에서 더 많은 여성고객 유치를 위해서는 공간과 미디어파사드의 조화, 형태, 색, 배치 등의 아름다움, 그리고 콘텐츠의 연출방식 등에 대해 여성을 고려해야 할 것이다.

• 한국세라믹학회지
• /
• 제40권12호
• /
• pp.1170-1176
• /
• 2003

진화최적방법을 이용하여 alkali earth borosilicate 계열(Eu, Mg, Ca, Sr, Ba)$_{x}$ $B_{y}$S $i_{z}$ $O_{d}$에 E $u^{3+}$ 를 도핑 하여 고효율 적색 형광체를 합성하였다. 본 연구는 삼원색 백색 LED로의 적용을 목적으로 한다. 진화최적방법은 유전알고리즘과 조합화학을 연계하여, LED형광체 개발을 위해 개발하였다. 유전알고리즘을 조합화학에 접목함으로써 시간과 자원의 낭비 없이 매우 효율적인 형광체 탐색을 꾀할 수 있었다. 실질적인 실험에 앞서 다양한 목적함수를 이용하여 시뮬레이션을 실시하여 본 연구의 타당성을 증명하고 실제 합성한 결과 삼원색 백색 LED용 적색형광체(E $u_{0.14}$M $g_{0.18}$C $a_{0.07}$B $a_{0.12}$ $B_{0.17}$S $i_{0.32}$ $O_{{\delta}}$)를 얻었다.얻었다.다.얻었다.얻었다.다.