• Journal of The Korean Society of Grassland and Forage Science
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• v.30 no.1
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• pp.1-8
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• 2010

Few management studies have been conducted on late planting date for corn silage in paddy field as a barley-corn forage cropping system. Experiments were conducted during 2007 and 2008 at the Chungnam Agricultural Research & Extension Services. The objectives of this study were to determine relationships between planting date and com forage yield and to determine the best hybrid at the delayed planting after whole crop barley's harvest. The treatments consisted of 2 planting dates and 7 hybrids. Delayed planting considerately reduced stem diameter, individual ear size and weight. The ratio of dried leaf and culm in aerial plants was increased and that of ear was decreased. The coefficient of variation in fresh, dry matter (DM) and total digestible nutrients (TDN) yield was higher at delayed planting date on cropping system with whole crop barley than that of planting at optimum season. So, fresh weight was reduced by 91.8%, dry matter by 72.6%, grain yield by 51.0% and TDN yield by 68.1%. The appropriate hybrid for delayed planting after whole crop barley harvest in middle region was 'Gangdaok' as lower in the reduction of ear size and weight compared to that of planting at optimum season. Reduction in grain yield caused to decrease the DM and TDN yields. Therefore, to gain stable dry matter in silage corn by delayed planting date on cropping system with whole crop barley was necessary to select com hybrid on the minimum reduction in ear size and weight.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.45 no.1
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• pp.55-58
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• 2000

Germination of sweet and super sweet corn is lower than normal corn due to the higher sugar and lower starch contents of kernels. Sweet corn seeds are easily deteriorated in the field under the unfavorable condition, therefore it is important to identify the optimal harvesting time for seed production. This trial was conducted to investigate the responses of germination percentage of shrunken-2(sh2), brittle(bt), sugary(su), and sugary enhancer(se) hybrids in relation to harvesting dates. Eight hybrids of four different gene sweet corns were harvested at 15, 20, 25, 30, 35, 40, 45, and 50 days after silking(DAS). Germination test was performed using paper towel method. Mean germination percentages across eight hybrids showed the highest value at 45 DAS. There were significant differences among genes and within gene for germination. Shrunken-2 hybrid Mecca was higher than su hybrids for germination, indicating that sh2 would not be poorer than su Late harvesting beyond the optimal harvesting date might not be desirable because of more lodging and ear rots. Theoretical optimal harvesting date estimated from the regression equation was 40.9 DAS, however, practical date for harvesting would be a few days later than the estimated date if seedling vigor might be considered. Kernel dry weight per ear showed similar response to germination. Regression equation showed the highest kernel dry weight at 40.7 DAS. Significant correlations between kernel dry weight and germination were observed, impling that kernel dry matter accumulation would be an important factor for germination.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.36 no.1
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• pp.52-56
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• 1991

In 1986, at Cheju and Seoguipo, a sweet com hybrid, Golden Cross Bantam 70, was grown at 5 phosphate rates (0, 8, 16, 24 and 32 kg/l0a) to determine effects of phosphate rate on growth and yield of sweet com and to recommend the optimum phosphate rate for sweet com production in volcanic ash soils of Cheju province. Days to silking were shortened 1 to 2 days by phosphate application of more than l6kg/10a. Culm length, ear height and fresh stover yield/l0a did not differ between two locations, but linearly increased as phosphate rate was increased from 0 to 32kg/10a. Average length and weight of marketable ears were greater at Cheju than at Seoguipo and linearly increased as phosphate rate increased. The number of marketable ears per plant and per l0a did not differ between two locations, but quadratically increased as phosphate rate increased. The economic optimum phosphate rate estimated on the basis of the quadratic model, ranged 25.6 to 27.4kg/10a at various fertilizer-to-ear price ratios.

• Proceedings of the Korean Society of Crop Science Conference
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• 2022.10a
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• pp.317-317
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• 2022

Seakso 5, a maize hybrid, was applied for variety in 2021 by Gangwon Agricultural Research and Extension Services in Korea. This variety was bred to produce a purple extract of com husk. It shows purple color in the husk and cob and contains abundant anthocyanins. In this study, to obtain basic data on purple com 'Seakso 5' husk extract (PCHE), a husk extract was prepared and the contents of vitamins, amino acids and fatty acids were analyzed. The Seakso 5 husks were extracted with 30% alcohol and concentrated, after adding dextrin to the concentrate, it was spray-dried to prepare an extract. The PCHE were analyzed for vitamins, amino acids, and fatty acids by referring to the Food Code. As a result, three types of vitamins were detected, and their contents were vitamin B₁ 0.14 mg/100g, vitamin B₂ 2.30 mg/100g, and vitamin C 11.32 mg/100g. A total of 15 amino acids were detected in the PCHE, including aspartic acid and glutamic acid, and the content of aspartic acid was the highest at 3.02 mg/g. Simultaneous analysis of fatty acids Among 37 types, saturated fatty acids were palmitic acid (C16:0), stearic acid (C18:0), monounsaturated fatty acids were oleic acid (C18:1(n-9)), polyunsaturated fatty acids were linoleic acid(C18:2(n-6)c) and a-Linolenic Acid (C18:3(n-3)) were detected. Among the detected fatty acids, the content of palmitic acid was the highest at 40.75 mg/100g. In the future, These analysis results will be used as reference data for temporary food ingredient applications by the Ministry of Food and Drug Safety.

• Journal of The Korean Society of Grassland and Forage Science
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• v.29 no.1
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• pp.13-18
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• 2009

This experiment was carried out to know adaptability and forage production and quality of corn hybrid for silage at paddy field from 2007 to 2008 at JullaNamdo Agricultural Research and Extension Services (ARES). Among agronomic characteristics, 'Kwangpyongok' hybrid was somewhat strong for waterlogging and good stay green, lodging, disease and insect resistance. The fresh yield and total digestible nutrients (TDN) of 'Kwangpyongok' hybrid were the highest among corn hybrids. Dry yield of 'Kwangpyongok' hybrid was increased to 50.5%, 13.6% due to waterlogging tolerance than those 'P3156' and 'P32P75' hybrids, respectively. The result of this study indicated that 'Kwangpyongok' hybrid could be recommended as having good characters according to production, waterlogging tolerance, stay green at paddy field in southern region of Korea.

• Animal cells and systems
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• v.6 no.4
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• pp.317-322
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• 2002

To improve conventional yeast one-hybrid screening, we have developed an efficient mammalian one-hybrid system that allows rapid isolation of com-plementary DNAs which are able to induce human p14$^{ARF}$. tumor suppressor gene. A 1.5 kb promoter region of p14$^{ARF}$ was fused to EGFP to generate ARF promoter-EGFP reporter vector. This reporter plasmid was stably trans-fected into NIH3T3 cells for generation of reporter cell line. When the reporter cell line was infected with E2F-1 together with excess amounts of empty vector, the cells that received the positive modulator were readily identifiable by green fluorescence using FACS. The GFP-positive cells were cloned directly from the cultured cells and expanded in bulk culture. The genomic DNAs from GFP-positive cells were prepared and the CDNA insert in integrated retroviral genome was recovered by PCR using primers annealing to the retroviral vector sequences flanking the insert-cloning site. This system should be useful for efficient screening of expression CDNA libraries in mammalian cells to identify novel upstream regulators for spe-cific genes by one-hybrid interaction.ion.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.28 no.4
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• pp.481-487
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• 1983

The need to synchronize flowering in two lines of different maturities is frequently encountered in hybrid com (Zea mays L.) seed production. To establish the methods for synchrony of flowering in parent lines of different maturities be effects of clipping at 4 and 6-leaf stages of growth and two levels, clear polyethylene mulching and five different planting dates on flowering date growth and seed yield of two dent com inbred lines of different maturities were evaluated Clipping just above the shoot-apex delayed pollen sheeding 6 to 9days and silking 5 to 13 days but reduced stand and seed yield 30 to 70% and 67 to 81%. respectively. Clipping 5cm above the shoot apex delayed flowering 1 to 4 days without stand reduction but reduced yield 3 to 29%. Laterclipping was slightly more effective for delaying flowering than earlier clipping but reduced stand more severely when clipped just above the shoot apex. Under clear polyethylene film mulching, flowering of two lines was 13 to 15 days earlier and seed yield of B68 (late line) was significantly increased. As planting was delayed from April 18 to June 13, the number of days from planting to flowering of two lines decreased due to increase in air temperature. However, growing degree days (GDD) from planting to flowering of each lines was similar regardless planting dates indicating that GDD can be satisfactoryly used for choosing the planting dates of parent lines of different maturities. Seed yields of two lines were decreased with delaying planting dates.

• Journal of The Korean Society of Grassland and Forage Science
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• v.22 no.4
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• pp.253-258
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• 2002

NC+4880, a forage com hybrid, is bred by NC+hybrids Co. in US, and Gast 8396 IT is by Garst Seeds Co. We have tested these two cultivars in Suwon and Chonan over three years to test their regional yield trial. Through above experiments, the excellence is testified, in result, they are determined as new recommended cultivars by NACF. The main characteristics of these cultivars are as tallows ; 1. NC+4880 a. The seed coat is yellow, the mean tasseling date is 7th of July and it takes about 74 days from emergence to tasseling date. This hybrid is a day ahead and the culm length is 242 cm, moreless shorter comparing with a check hybrid, Suwon 19. The ear height 117 cm which is short and its stay green is moderate. b. It shows resistance to H maydis, and MBSDV is barely observed and its stay green is moderate. c. The fresh yield, dry matter yield, and TDN yield per a ha are 55.4 tons, 18.3 tons, 12.4 tons, respectively, which are increased by 6%, 7%, and 9%, respectively, comparing with a check hybrid, Suwon 19. The percent ear among total dry matter yield is 50.9%. 2. Garst 8396 IT a. The seed coat is yellow, the mean tasseling date is 9th of July and it takes about 76 days from emergence to tasseling date. This hybrid represent a day delayed compared with a check hybrid, Suwon 19. The Culm length and the ear height are 275 cm, and 138 cm, respectively. b. It resists moderately to H. maydis, and highly resists to lodging in the field conditions. MBSDV is barely observed and its stay green is moderate. c. The fresh yield per a ha is 64.9 tons, dry matter yield, 20.9 tons, and TDN, 13.8 tons, respectively, which are increased by 25%, 23%, and 22% comparing with a check hybrid, Suwon 19. The percent ear among total dry matters is 43.3%.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.53 no.4
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• pp.606-619
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• 2020

This study investigated the effects of crude protein levels in diets containing monobasic potassium phosphate (MKP) on water quality and the growth of Japanese eels Anguilla japonica and leafy vegetables in a Hybrid BFT-Aquaponics (HBFT-AP) system. The first experiment (EXP1) was designed to verify the effects of the feed itself on leafy vegetable productivity using two diets (CP48 and CP30) with MKP and one commercial eel diet (COM58). The second experiment (EXP2) examined the effects of the three diets on productivity of the fish and leafy vegetables in the HBFT-AP for 6 weeks. After the 6 week feeding trial, the weight gain, feed efficiency, specific growth rate and protein efficiency ratio of fish fed COM58 and CP48 were higher than those of fish fed CP30 (P<0.05) and the growth of the four leafy vegetables was the highest with fish fed CP48. Water quality was measured six times per week using a portable water quality meter and reagent measurements and showed variance with time for TAN (0.01-0.09 mg/L), NO₂-N (0.010-0.064 mg/L), NO₃-N (5.52-27.15 mg/L), PO₄-P (2.03-5.32 mg/L) and pH (7.86-6.15).

• Proceedings of the KIEE Conference
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• 2006.10c
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• pp.120-122
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• 2006

The paper concerns the hybrid optimization of fuzzy inference systems that is based on Hierarchical Fair Competition-based Parallel Genetic Algorithms (HFCGA) and information data granulation. The granulation is realized with the aid of the Hard C-means clustering and HFCGA is a kind of multi-populations of Parallel Genetic Algorithms (PGA), and it is used for structure optimization and parameter identification of fuzzy model. It concerns the fuzzy model-related parameters such as the number of input variables to be used, a collection of specific subset of input variables, the number of membership functions, the order of polynomial, and the apexes of the membership function. In the hybrid optimization process, two general optimization mechanisms are explored. Thestructural optimization is realized via HFCGA and HCM method whereas in case of the parametric optimization we proceed with a standard least square method as well as HFCGA method as well. A comparative analysis demonstrates that the proposed algorithm is superior to the conventional methods.