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검색결과 319건 처리시간 0.023초

The Study of Mutation Spectrum in Iac / Gene of Transgenic Big Blue$\textregistered$ Cell Line Following Short-Term Exposure to 4-Nitroquinoline N-oxide

  • Youn, Ji-Youn;Kim, Kyung-Ran;Cho, Kyung-Hea;Ryu, Jae-Chun
    • 한국환경독성학회:학술대회논문집
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    • 한국환경독성학회 1996년도 제19회정기학술대회(The 19th Symposium of the Korean Society of Environmental Toxicology)
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    • pp.64-64
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    • 1996
  • Transgenic animal and cell line models which are recently developed in toxicology field combined with molecular biological technique, are powerful tools for studying of mutation in vivo and in vitro, respectively. The Big Blue mutagenesis assay system is one of the most widely used transgenic systems. Especially, for the study of direct acting mutagens, Big Blue cell line is very useful and powerful to evaluate mutagenicity because the mutation frequency and mutationspectrlun showed no distinct differences between cell line and animal. The Big Blue cell lines carry stably integrated copies of lambda shuttle vector containing lac I gene as a mutational target. These lambda shuttle vectors are useful for various mutagenesis related studies in eukaryotic system due to their ability to be exposed mutagen and then transfer a suitable target DNA sequence to it convenient organism for analysis. We tried to assess the mutagenic effect of 4-NQO with Big Blue cell line. After the treatment of 4-NQO, genomic DNA was isolated and lambda shuttle vector was packaged by in Vitro packaging and then these were plated on bacterial host in the presence of X-gal to screen mutation in the lac I. We determined MF as a ratio of blue plaques versus colorless plaques and now undergoing the mutation spectrum of 4-NQO in lac J gene sequence.

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가평 사문암 광산의 석면 산출의 산상과 모암의 특성 (Occurrences of Asbestos within Gapyeong Serpentinite Mines and Characteristics of Host Rocks)

  • 송석환;강준구
    • 한국산업보건학회지
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    • 제26권3호
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    • pp.253-266
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    • 2016
  • Objectives: This study is for characteristics of asbestos occurrence (NOA, naturally occurring asbestos) from the Gapyeong area and its host rocks, serpentinites. Methods: Representative samples are collected from the serpentinite bodies, following degrees of hydrothermal alteration and metamorphism, after about 2 year field trips. Mineralogical, morphological and optical characteristics of the asbestos and host rocks are confirmed by PLM, XRD EPMA and SEM results. Results: The serpentinites are dunites and harzburgites, and host asbestos, including chrysotile, tremolite and actinolite. The asbestos chrysotiles are found as veins ranging from several millimeters to several centimeters in thickness, while asbestos-tremolite and -actinolite occur along cracks and fractures ranging up to ten centimeters in thickness. The chryostiles occur mainly as cross and slip fibers, while the amphibole asbestos is found as vein, slip and oblique fibers. More tremolitic grains are colorless and commonly show elongated or fiber shapes, whereas the magnesio hornblende grains mainly show light green and occur as subhedral to euhedral diamond grains. Conclusions: Overall characteristics of serpentinites from the Gapyeong area are similar to worldwide orogenic-related Alpine type ultramafic rocks serpentinized and serpentinites in South Chungcheong-do Province, Korea, and occurrences of asbestos are similar to those of the ultramafic bodies in South Chungcheong-do Province.

Botrytis cinerea에 의한 바이올렛 잿빛곰팡이병 (Gray Mold on Saintpaulia ionantha Caused by Botrytis cinerea in Korea)

  • 김형무
    • 식물병연구
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    • 제17권1호
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    • pp.75-77
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    • 2011
  • 전북 전주시 화원에서 Botrytis cinerea에 의한 바이올렛 잿빛곰팡이병이 발생하였다. 병징은 꽃, 잎과 잎자루가 수침상으로 물러지고 부패되었다. 다습조건에서 병반위에 잿빛색의 분생포자가 많이 형성하였다. 균총은 PDA 배지에서 회갈색이며 균핵을 형성하였다. 분생포자는 단포자, 난형 또는 타원형으로 무색 또는 옅은 갈색이고, 크기는 $7{\sim}14{\times}5{\sim}9\;{\mu}m$이었다. 바이올렛에 발생한 병징과 병원균의 균학적 특징을 조사한 결과 B. cinerea Persoon:Fries에 의한 바이올렛 잿빛곰팡이병으로 명명할 것을 제안한다.

친화막의 L-tryptophan 흡착특성 (Adsorption Characteristic of L-tryptophan of Affinity Membrane)

  • 변홍식;홍병표
    • 멤브레인
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    • 제18권3호
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    • pp.214-218
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    • 2008
  • 전기방사를 이용하여 polyethersulfone (PES)-bovin albumin serum (BSA) 단백질 친화막을 제조하였으며, 이때 전기방사에 의한 공정상의 문제점을 용해도가 높고, 끓는점이 높은 2,2,3,4,4,4-Hexafluoro-1-butanol (HFB)를 공용매로 사용함으로써 해결하였다. 또한 공용매는 최적의 온도, 습도 범위를 확대시켜줌으로써 친화막의 대량 생산이 용이하리라 생각한다. 제조된 친화막은 biuret test를 통하여 친화막의 색깔이 무색에서 보라색으로 변함으로써 PES 섬유 내 BSA가 존재함을 확인할 수 있었다. 완충용액의 조성 성분 중 dimethyl sulfoxide (DMSO)는 세척과정에서 BSA와 L-tryptophan사이의 해리를 제어함으로써 용출과정에서 비교실험보다 약 5배의 높은 용출량을 보여주었다.

Colletotrichum acutatum에 관한 의한 탄저병 (Safflower (Carthamus tinctorius L.) Anthracnose Casued by Colletotrichum acutatum in Korea)

  • 권진혁;강수웅;김희규;박창석
    • 한국식물병리학회지
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    • 제14권6호
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    • pp.651-653
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    • 1998
  • A destructive anthracnose of safflower has severely occurred at Kyeongsangnam-do Agricultural Research and Extention Services in 1998. Incidence of the disease surveyed at 12 fields in Chinju, Sanchong and Hamyang was ranged fro 55.7 to 85.4%. Small irregular yellowish to brown spots appeared on all parts of the plant at initial infection stage and the spots enlarged or united into larger irregular to cylindrical black lesions with hollows. Above portions of infected stems were often blighted and pink colored conidial masses were colonized on the lesions. The causal fungus consistently isolated from the infected tissues were identified as Colletotrichum acutatum by following characteristics. The fungus grew well on PDA at 15~3$0^{\circ}C$ developing apricot to salmon color. Conidia were single-celled, colorless, fusiform and bisectioned and measured as 8~18$\times$3~5 ${\mu}{\textrm}{m}$. Brownish appressoria were cylindrical or ovate and sized as 5~10$\times$4~8 ${\mu}{\textrm}{m}$. Optimum temperature for growth and germination of conidia was recorded at $25^{\circ}C$ and at least 6 hours were required for infection to the host plants. Over 90% conidia were germinated and all plants were infected by artificial inoculation by 24 hours at $25^{\circ}C$. This is the first report of safflower anthracnose caused by C. acutatum in Korea.

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염산(鹽酸)Promazine 주사제(注射劑)의 안정화(安定化)에 관한 연구(硏究) (Study on Stabilization of Promazine Hydrochloride Injection)

  • 이완하;지웅길;양재헌
    • Journal of Pharmaceutical Investigation
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    • 제3권3호
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    • pp.5-15
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    • 1973
  • The stabilizing effect of promazine hydrochloride injectin was tested by irradiation of sun lamp(5,000 Lux) on their acid solution sealed in ampules under various stabilizers. The result of experimentation on the color change, the remaining proportion and the half life of promazine hydrochloride solution is as follows. 1. Instability of promazine hydrochloride solution is mainly caused by the sunlight and oxygen. 2. The pH range of promazine hydrochloride injection is recommended to $pH\;4.4{\sim}5.2$ 3. The decomposition of promazine hydrochloride by the sunlight is composed of pseudo zero order reaction when It maintains colorless solution. 4. The half life of 0.5% promazine hydrochloride injection was 30 hrs. under sun lamp irradiation, but it was delayed to 84 hrs. by the simple stabilizer and to 175 hrs. by compound stabilizer. 5. The stabilizing effect of promazine .hydrochloride injection adding sod. metabisulfite$(Na_2S_2O_5)$ was most excellent in various simple stabilizers and adding $Na_2S_2O_5$ to nicotinic acid, $Na_2S_2O_5$ to nicotinamide were more excellent than other compound stabilizers.

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Comparison between the Color Properties of Whiteness Index and Yellowness Index on the CIELAB

  • Jung, Hyojin;Sato, Tetsuya
    • 한국염색가공학회지
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    • 제25권4호
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    • pp.241-246
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    • 2013
  • The color properties of a white or nearly colorless fabric are represented by whiteness index (WI) or yellowness index (YI). These two indexes relate to a white fabric's color quality. The purpose of this study was to identify the properties of WI and YI on the CIELAB through the simulations of estimation data for a systemization of color quality control. The results indicated that the relationship between WI and YI was a negative correlation, however the coefficients of correlation function between WI and YI were depended on hue. There were two hue transition points of the rate of changes in WI and YI. These hue transition points were the reference points to divide the hue contribution to WI and YI. These points were not the point of h=0 and h=180 and asymmetric. In addition, where the colors were same distance from the white point on the CIELAB, the rate of changes in WI and YI by ${\Delta}$Ew were depending on hue. Specifically, when WI decreased, YI of reddish and yellowish tinted colors decreased more than bluish tinted colors.

안트라사이트를 혼입한 시멘트 보드의 라돈흡착 특성 (Radon adsorption properties of cement board using anthracite)

  • 경인수;편수정;이상수
    • 한국건축시공학회:학술대회논문집
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    • 한국건축시공학회 2018년도 춘계 학술논문 발표대회
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    • pp.232-233
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    • 2018
  • Among the recent environmental pollution, indoor air pollution has an adverse effect on the health of indoor residents. Radon, one of the causes of indoor air pollution, is released from concrete, gypsum board and asbestos slate among building materials. Radon is a primary carcinogen and is a colorless, tasteless, odorless inert gas that adheres to airborne dust and enters the body through breathing. At this time, there is a risk of developing cancer if the alpha rays from the lononggas entering the human body destroys the lung tissue and is continuously exposed to a high concentration of lonon gas. The World Health Organization (WHO) has emphasized the reduction of radon and its exposure to radon by classifying it as a first-level carcinogen, but many people have not recognized it yet, and the research is underdeveloped. Therefore, this study was carried out to investigate the properties of adsorbed coconut radon to prevent the inflow of radon gas, which is an air pollution source of indoor air, and to prevent inflow into the human body.

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초대형 막구조물 지붕용 ETFE 필름 막재의 인장특성 (Tensile Strength Characteristics of ETFE Roof Material in Large Membrane Structuresb)

  • 이승재;이소라
    • 한국공간구조학회논문집
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    • 제10권1호
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    • pp.51-58
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    • 2010
  • ETFE 필름 막재는 Ethylen Tetra Fluoro Etylene의 약자로, 무색의 투명한 막재료이다. ETFE 필름 막재는 내화학성이 있고 매우 가벼운 장점이 있는 것으로 알려져 있다. ETFE 필름 막재는 $50{\mu}m$에서 $300{\mu}m$의 두께가 주로 사용되며, 인장강도는 40MPa에서 60MPa 300%에서 400%정도이다. 본 연구에서는 최근 해외에서 초대형 박구조물의 박재료로 시용되는 ETFE 필름 막재에 대한 기초적 역학적 특성을 조사하기 위하여 인장시험을 수행하였다. 인장시험으로부터 인장강도, 인장연신율, 영계수(Young's Modulus) 등 건축 설계 시 필요한 기초적 자료를 제공하고자 한다.

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돈육으로 제조한 정제단백질과 Oligopeptide의 영양평가 : 소화율, 생물가, 단백질 실이용률 (Purified Protein and Oligopeptide Mixture Preparation from Pork Meat and Evaluation of their Nutritive Value: True Digestibility, Biological Value, and Net Protein Utilization)

  • 김종희;손민희;조정순
    • 한국식품조리과학회지
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    • 제23권5호
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    • pp.644-649
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    • 2007
  • In this study a purified protein was prepared from pork meat. The product consisted of 0.5% moisture, 3.0% ash, 5.5% ether extract and 88.7% crude protein. Also, a meat oligopeptide mixture was prepared from a pepsin digest of the protein preparation. The two preparations were colorless and odorless powders with low fat contents. The nutritive values of the pork meat protein and oligopeptide mixture were estimated by two methods, one using biological value(BV) and the other employing net protein utilization(NPU) by the nitrogen balance method. The meat oligopeptide mixture showed an excellent nutritive value by both methods. The true digestibilites of both the pork meat protein and the oligopeptide mixture were more than 98%. The above results indicate that the oligopeptide mixture is an excellent material as a dietary nitrogen source for many purposes.