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The Study of Mutation Spectrum in Iac / Gene of Transgenic Big Blue$\textregistered$ Cell Line Following Short-Term Exposure to 4-Nitroquinoline N-oxide

  • Youn, Ji-Youn;Kim, Kyung-Ran;Cho, Kyung-Hea;Ryu, Jae-Chun
    • Proceedings of the Korea Society of Environmental Toocicology Conference
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    • 1996.12a
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    • pp.64-64
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    • 1996
  • Transgenic animal and cell line models which are recently developed in toxicology field combined with molecular biological technique, are powerful tools for studying of mutation in vivo and in vitro, respectively. The Big Blue mutagenesis assay system is one of the most widely used transgenic systems. Especially, for the study of direct acting mutagens, Big Blue cell line is very useful and powerful to evaluate mutagenicity because the mutation frequency and mutationspectrlun showed no distinct differences between cell line and animal. The Big Blue cell lines carry stably integrated copies of lambda shuttle vector containing lac I gene as a mutational target. These lambda shuttle vectors are useful for various mutagenesis related studies in eukaryotic system due to their ability to be exposed mutagen and then transfer a suitable target DNA sequence to it convenient organism for analysis. We tried to assess the mutagenic effect of 4-NQO with Big Blue cell line. After the treatment of 4-NQO, genomic DNA was isolated and lambda shuttle vector was packaged by in Vitro packaging and then these were plated on bacterial host in the presence of X-gal to screen mutation in the lac I. We determined MF as a ratio of blue plaques versus colorless plaques and now undergoing the mutation spectrum of 4-NQO in lac J gene sequence.

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Occurrences of Asbestos within Gapyeong Serpentinite Mines and Characteristics of Host Rocks (가평 사문암 광산의 석면 산출의 산상과 모암의 특성)

  • Song, Suckhwan;Kang, Joongu
    • Journal of Korean Society of Occupational and Environmental Hygiene
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    • v.26 no.3
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    • pp.253-266
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    • 2016
  • Objectives: This study is for characteristics of asbestos occurrence (NOA, naturally occurring asbestos) from the Gapyeong area and its host rocks, serpentinites. Methods: Representative samples are collected from the serpentinite bodies, following degrees of hydrothermal alteration and metamorphism, after about 2 year field trips. Mineralogical, morphological and optical characteristics of the asbestos and host rocks are confirmed by PLM, XRD EPMA and SEM results. Results: The serpentinites are dunites and harzburgites, and host asbestos, including chrysotile, tremolite and actinolite. The asbestos chrysotiles are found as veins ranging from several millimeters to several centimeters in thickness, while asbestos-tremolite and -actinolite occur along cracks and fractures ranging up to ten centimeters in thickness. The chryostiles occur mainly as cross and slip fibers, while the amphibole asbestos is found as vein, slip and oblique fibers. More tremolitic grains are colorless and commonly show elongated or fiber shapes, whereas the magnesio hornblende grains mainly show light green and occur as subhedral to euhedral diamond grains. Conclusions: Overall characteristics of serpentinites from the Gapyeong area are similar to worldwide orogenic-related Alpine type ultramafic rocks serpentinized and serpentinites in South Chungcheong-do Province, Korea, and occurrences of asbestos are similar to those of the ultramafic bodies in South Chungcheong-do Province.

Gray Mold on Saintpaulia ionantha Caused by Botrytis cinerea in Korea (Botrytis cinerea에 의한 바이올렛 잿빛곰팡이병)

  • Kim, Hyung-Moo
    • Research in Plant Disease
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    • v.17 no.1
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    • pp.75-77
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    • 2011
  • Gray mold caused by Botrytis cinerea occurred on Saintpaulia ionantha in flower shop of the Jeonju city in Korea. Typical symptoms with brown water-soaked and rotting lesions were appeared on the flowers, leaves and petiole of infected plants. Many conidia spores appeared on the lesions under humid conditions. Colonies were grayish brown and sclerotial formation on potato dextrose agar. Conidia were one celled, mostly ellipsoidal or ovoid in shape, and were colorless to pale brown in color. The conidia were $7{\sim}14{\times}5{\sim}9\;{\mu}m$ in size. Based on pathogenicity and morphological characteristics of the isolated fungus, the causal fungus was identified as B. cinerea Persoon: Fries. Gray mold of S. ionantha was proposed to the name of this disease.

Adsorption Characteristic of L-tryptophan of Affinity Membrane (친화막의 L-tryptophan 흡착특성)

  • Byun, Hong-Sik;Hong, Byung-Pyo
    • Membrane Journal
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    • v.18 no.3
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    • pp.214-218
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    • 2008
  • Protain affinity membranes based on PES-BSA was preapared by the electrospinning method. The process problem caused by the electrospining was solved by using HFB having high solubility and boiling point. It was expecting that the mass production of protein affinity membrane would be possible with broad range of optimized temperature and humidity. BSA in the PES nanofiber was confirmed by the color change from colorless to violet during the biuret test. The buffer solution with DMSO showed that the amount of elution was 5 times higher than the one when the buffer solution without DMSO was used. This is due to the restriction effect of DMSO on the dissociation of L-tryptophan from BSA during the washing step.

Safflower (Carthamus tinctorius L.) Anthracnose Casued by Colletotrichum acutatum in Korea (Colletotrichum acutatum에 관한 의한 탄저병)

  • 권진혁;강수웅;김희규;박창석
    • Korean Journal Plant Pathology
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    • v.14 no.6
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    • pp.651-653
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    • 1998
  • A destructive anthracnose of safflower has severely occurred at Kyeongsangnam-do Agricultural Research and Extention Services in 1998. Incidence of the disease surveyed at 12 fields in Chinju, Sanchong and Hamyang was ranged fro 55.7 to 85.4%. Small irregular yellowish to brown spots appeared on all parts of the plant at initial infection stage and the spots enlarged or united into larger irregular to cylindrical black lesions with hollows. Above portions of infected stems were often blighted and pink colored conidial masses were colonized on the lesions. The causal fungus consistently isolated from the infected tissues were identified as Colletotrichum acutatum by following characteristics. The fungus grew well on PDA at 15~3$0^{\circ}C$ developing apricot to salmon color. Conidia were single-celled, colorless, fusiform and bisectioned and measured as 8~18$\times$3~5 ${\mu}{\textrm}{m}$. Brownish appressoria were cylindrical or ovate and sized as 5~10$\times$4~8 ${\mu}{\textrm}{m}$. Optimum temperature for growth and germination of conidia was recorded at $25^{\circ}C$ and at least 6 hours were required for infection to the host plants. Over 90% conidia were germinated and all plants were infected by artificial inoculation by 24 hours at $25^{\circ}C$. This is the first report of safflower anthracnose caused by C. acutatum in Korea.

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Study on Stabilization of Promazine Hydrochloride Injection (염산(鹽酸)Promazine 주사제(注射劑)의 안정화(安定化)에 관한 연구(硏究))

  • Lee, Wan-Ha;Chi, Ung-Kil;Young, Jae-Hun
    • Journal of Pharmaceutical Investigation
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    • v.3 no.3
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    • pp.5-15
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    • 1973
  • The stabilizing effect of promazine hydrochloride injectin was tested by irradiation of sun lamp(5,000 Lux) on their acid solution sealed in ampules under various stabilizers. The result of experimentation on the color change, the remaining proportion and the half life of promazine hydrochloride solution is as follows. 1. Instability of promazine hydrochloride solution is mainly caused by the sunlight and oxygen. 2. The pH range of promazine hydrochloride injection is recommended to $pH\;4.4{\sim}5.2$ 3. The decomposition of promazine hydrochloride by the sunlight is composed of pseudo zero order reaction when It maintains colorless solution. 4. The half life of 0.5% promazine hydrochloride injection was 30 hrs. under sun lamp irradiation, but it was delayed to 84 hrs. by the simple stabilizer and to 175 hrs. by compound stabilizer. 5. The stabilizing effect of promazine .hydrochloride injection adding sod. metabisulfite$(Na_2S_2O_5)$ was most excellent in various simple stabilizers and adding $Na_2S_2O_5$ to nicotinic acid, $Na_2S_2O_5$ to nicotinamide were more excellent than other compound stabilizers.

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Comparison between the Color Properties of Whiteness Index and Yellowness Index on the CIELAB

  • Jung, Hyojin;Sato, Tetsuya
    • Textile Coloration and Finishing
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    • v.25 no.4
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    • pp.241-246
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    • 2013
  • The color properties of a white or nearly colorless fabric are represented by whiteness index (WI) or yellowness index (YI). These two indexes relate to a white fabric's color quality. The purpose of this study was to identify the properties of WI and YI on the CIELAB through the simulations of estimation data for a systemization of color quality control. The results indicated that the relationship between WI and YI was a negative correlation, however the coefficients of correlation function between WI and YI were depended on hue. There were two hue transition points of the rate of changes in WI and YI. These hue transition points were the reference points to divide the hue contribution to WI and YI. These points were not the point of h=0 and h=180 and asymmetric. In addition, where the colors were same distance from the white point on the CIELAB, the rate of changes in WI and YI by ${\Delta}$Ew were depending on hue. Specifically, when WI decreased, YI of reddish and yellowish tinted colors decreased more than bluish tinted colors.

Radon adsorption properties of cement board using anthracite (안트라사이트를 혼입한 시멘트 보드의 라돈흡착 특성)

  • Kyoung, In-Soo;Pyeon, Su-Jeong;Lee, Sang-Soo
    • Proceedings of the Korean Institute of Building Construction Conference
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    • 2018.05a
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    • pp.232-233
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    • 2018
  • Among the recent environmental pollution, indoor air pollution has an adverse effect on the health of indoor residents. Radon, one of the causes of indoor air pollution, is released from concrete, gypsum board and asbestos slate among building materials. Radon is a primary carcinogen and is a colorless, tasteless, odorless inert gas that adheres to airborne dust and enters the body through breathing. At this time, there is a risk of developing cancer if the alpha rays from the lononggas entering the human body destroys the lung tissue and is continuously exposed to a high concentration of lonon gas. The World Health Organization (WHO) has emphasized the reduction of radon and its exposure to radon by classifying it as a first-level carcinogen, but many people have not recognized it yet, and the research is underdeveloped. Therefore, this study was carried out to investigate the properties of adsorbed coconut radon to prevent the inflow of radon gas, which is an air pollution source of indoor air, and to prevent inflow into the human body.

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Tensile Strength Characteristics of ETFE Roof Material in Large Membrane Structuresb (초대형 막구조물 지붕용 ETFE 필름 막재의 인장특성)

  • Lee, Seung-Jae;Lee, So-Ra
    • Journal of Korean Association for Spatial Structures
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    • v.10 no.1
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    • pp.51-58
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    • 2010
  • ETFE is the abbreviation of Ethylen Tetra Fluoro Etylene, a sort of colorless and transparent granules. The advantage ETFE film has daylight transmission and chemical the resistance and The thickness of ETFE film is used to from $50{\mu}m$ to $300{\mu}m$ and tensile strength of ETFE film changes from 40MPa to 60MPa and the tensile strain at break can get to about 300-400%. In this paper, ETFE film carried out the tensile proprieties, such as the tensile strain at break, the tensile strength are examined.

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Purified Protein and Oligopeptide Mixture Preparation from Pork Meat and Evaluation of their Nutritive Value: True Digestibility, Biological Value, and Net Protein Utilization (돈육으로 제조한 정제단백질과 Oligopeptide의 영양평가 : 소화율, 생물가, 단백질 실이용률)

  • Kim, Jong-Hee;Son, Min-Hee;Cho, Jung-Soon
    • Korean journal of food and cookery science
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    • v.23 no.5
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    • pp.644-649
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    • 2007
  • In this study a purified protein was prepared from pork meat. The product consisted of 0.5% moisture, 3.0% ash, 5.5% ether extract and 88.7% crude protein. Also, a meat oligopeptide mixture was prepared from a pepsin digest of the protein preparation. The two preparations were colorless and odorless powders with low fat contents. The nutritive values of the pork meat protein and oligopeptide mixture were estimated by two methods, one using biological value(BV) and the other employing net protein utilization(NPU) by the nitrogen balance method. The meat oligopeptide mixture showed an excellent nutritive value by both methods. The true digestibilites of both the pork meat protein and the oligopeptide mixture were more than 98%. The above results indicate that the oligopeptide mixture is an excellent material as a dietary nitrogen source for many purposes.