• Title/Summary/Keyword: Color degradation

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Combined Gain Analysis of L-band Transmit Antenna in COMS (COMS L-대역 송신 안테나 합성 이득 해석)

  • Kim, Joong-Pyo;Yang, Koon-Ho;Lee, Sang-Kon
    • Journal of Satellite, Information and Communications
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    • v.5 no.2
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    • pp.19-24
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    • 2010
  • The COMS (Communication Ocean Meteorological Satellite) is a hybrid geostationary satellite including communication, ocean, and meteorological payloads. The COMS includes the MODCS (Meteorological and Ocean Data Communication Subsystem) which provides transmitting the raw data collected by meteorological payload called MI (Meteorological Imager) and ocean payload named GOCI (Geostationary Ocean Color Imager) to the ground station, and relaying the meteorological data processed on the ground to the end-user stations. Here, for the L-band transmit antenna transmitting SD (Sensor Data) signal and the processed signal, from the system point of view, it is required to estimate the combined antenna gain when the L-band transmit is placed with MI and GOCI payloads on the earth panel of COMS. First of all, the L-band transmit horn is designed and analyzed for the requirements given, and then after placing it on the earth panel, the combined gain analysis is performed using three different analysis methods. It's shown that the obtained gain patterns are very similar among three different analysis methods. Finally the antenna gain degradation of less than 0.5 dB is estimated.

Effective Microwell Plate-Based Screening Method for Microbes Producing Cellulase and Xylanase and Its Application

  • Kim, Jennifer Jooyoun;Kwon, Young-Kyung;Kim, Ji Hyung;Heo, Soo-Jin;Lee, Youngdeuk;Lee, Su-Jin;Shim, Won-Bo;Jung, Won-Kyo;Hyun, Jung-Ho;Kwon, Kae Kyoung;Kang, Do-Hyung;Oh, Chulhong
    • Journal of Microbiology and Biotechnology
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    • v.24 no.11
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    • pp.1559-1565
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    • 2014
  • Cellulase and xylanase are main hydrolysis enzymes for the degradation of cellulosic and hemicellulosic biomass, respectively. In this study, our aim was to develop and test the efficacy of a rapid, high-throughput method to screen hydrolytic-enzyme-producing microbes. To accomplish this, we modified the 3,5-dinitrosalicylic acid (DNS) method for microwell plate-based screening. Targeted microbial samples were initially cultured on agar plates with both cellulose and xylan as substrates. Then, isolated colonies were subcultured in broth media containing yeast extract and either cellulose or xylan. The supernatants of the culture broth were tested with our modified DNS screening method in a 96-microwell plate, with a $200{\mu}l$ total reaction volume. In addition, the stability and reliability of glucose and xylose standards, which were used to determine the enzymatic activity, were studied at $100^{\circ}C$ for different time intervals in a dry oven. It was concluded that the minimum incubation time required for stable color development of the standard solution is 20 min. With this technique, we successfully screened 21 and 31 cellulase- and xylanase-producing strains, respectively, in a single experimental trial. Among the identified strains, 19 showed both cellulose and xylan hydrolyzing activities. These microbes can be applied to bioethanol production from cellulosic and hemicellulosic biomass.

A Study on the Photo-Degradation Properties of the Spiropyran Using THz-TDS (테라헤르츠 시간 영역 분광법을 이용한 스피로파이란의 광 퇴화 특성 연구)

  • Bang, Jin-Hyuk;Park, Myoung-Hwan;Ryu, Han-Cheol
    • Journal of the Korean Chemical Society
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    • v.60 no.1
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    • pp.28-33
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    • 2016
  • The spiropyran is a typical material having photodegradation properties in the process of photochromism. The spiropyran has been utilized in various applications such as optical switch, optical memories, and biosensor because of its remarkable stability, fast responsive time, stronger color change, and photo-induced controllability. However, the spriropyran is photodegraded by the repetitive optical irradiation. The photodegradation of spiropyran have been investigated by using UV-Visible spectroscopy, nuclear magnetic resonance (NMR), and Raman spectroscopy. Herein, the properties of spiropyran were characterized by using terahertz time-domain spectroscopy (THz-TDS) in the terahertz frequency region. In terahertz region, the measured absorbance of spiropyran was increased due to the photodegradation induced by the repetitive UV irradiation. The absorbance tendency of spiropyran in the terahertz frequency region was compared with that in the visible region, and they were completely opposite to each other.

A Study on Three-Dimensional Image Modeling and Visualization of Three-Dimensional Medical Image (삼차원 영상 모델링 및 삼차원 의료영상의 가시화에 관한 연구)

  • Lee, Kun;Gwun, Oubong
    • Journal of the Korea Computer Graphics Society
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    • v.3 no.2
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    • pp.27-34
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    • 1997
  • 3-D image modeling is in high demand for automated visual inspection and non-destructive testing. It also can be useful in biomedical research, medical therapy, surgery planning, and simulation of critical surgery (i.e. cranio-facial). Image processing and image analysis are used to enhance and classify medical volumetric data. Analyzing medical volumetric data is very difficult In this paper, we propose a new image modeling method based on tetrahedrization to improve the visualization of three-dimensional medical volumetric data. In this method, the trivariate piecewise linear interpolation is applied through the constructed tetrahedral domain. Also, visualization methods including iso-surface, color contouring, and slicing are discussed. This method can be useful to the correct and speedy analysis of medical volumetric data, because it doesn't have the ambiguity problem of Marching Cubes algorithm and achieves the data reduction. We expect to compensate the degradation of an accuracy by using an adaptive sub-division of tetrahedrization based on least squares fitting.

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Rapid Screening Method of Peroxidase by Colorimetric Assay and Screening of 2, 4-DCP Degradable Strains (발색법에 의한 Peroxidase의 신속한 스크리닝법과 2, 4-DCP 분해균주의 스크리닝)

  • Ryu, Kang;Lee, Eun-Kyu
    • KSBB Journal
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    • v.23 no.6
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    • pp.484-488
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    • 2008
  • Chlorinated phenols are widely used by the chemical industry as intermediate products in synthesis and previously were frequently applied to various industry fields. Peroxidases catalyze the peroxide-dependent oxidation of a range of inorganic and organic compounds. Peroxidase was shown to mineralize a variety of recalcitrant aromatic compounds and to oxidize a number of polycyclic aromatic and phenolic compounds. Among monomeric phenolic and nonphenolic compounds, peroxidase is known to oxidize its compounds. In this study, a colorimetric assay was developed to quantitatively evaluate the peroxidase activity for rapid screening. Color products of different intensity were developed proportionally to the peroxidase activity on agar plate and 96-well plate. This method correlates well with the RP-HPLC result. Using this screening method, 12 colonies of strain was screened which survived at high concentration of 2,4-DCP (1000 ppm) and with peroxidase activity for the $7^{th}$ round screening step on agar plate. These strains were utilized 2,4-DCP as a sole carbon source and produced peroxidase. After the screening test, four of the bacteria have significant better effect of COD removal on dye waste-water. COD removal of these was from 44% to 61%, respectively.

"A La Recherche" of Functions for the Spore Protein SASP-E from Bacillus subtilis

  • Ruzal, Sandra M.;Bustos, Patricia L.;Sanchez-Rivas, Carmen
    • Journal of Microbiology and Biotechnology
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    • v.23 no.1
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    • pp.15-21
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    • 2013
  • We previously observed that Bacillus subtilis spores from sspE mutants presented a lower germination capacity in media containing high salt concentrations (0.9M NaCl). This deficiency was attributed to the absence of SASP-E (gamma-type small-acid-soluble protein), rich in osmocompatible amino acids released by degradation. Herein we observed that, in addition, this mutant spore presented a reduced capacity to use L-alanine as germinant (L-ala pathway), required longer times to germinate in calcium dipicolinate ($Ca^{2+}$-DPA), but germinated well in asparagine, glucose, fructose, and potassium chloride (AGFK pathway). Moreover, mild sonic treatment of mutant spores partially recovered their germination capacity in L-ala. Spore qualities were also altered, since sporulating colonies from the sspE mutant showed a pale brownish color, a higher adherence to agar plates, and lower autofluorescence, properties related to their spore coat content. Furthermore, biochemical analysis showed a reduced partition in hexadecane and a higher content of $Ca^{2+}$-DPA when compared with its isogenic wild-type control. Coat protein preparations showed a different electrophoretic pattern, in particular when detected with antibodies against CotG and CotE. The complementation with a wild-type sspE gene in a plasmid allowed for recovering the wild-type coat phenotype. This is the first report of a direct involvement of SASP-E in the spore coat assembly during the differentiation program of sporulation.

Effect of a Refrigerator with LED on Functional Composition Changes and Freshness Prolongation of Cabbage (냉장고의 LED부착이 양배추의 선도 연장에 미치는 영향)

  • Park, Shin-Young;Chang, Min-Sun;Choi, Jung-Hee;Kim, Byeong-Sam;Lee, Hye-Ran;Ham, Kyung-Hee
    • Food Science and Preservation
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    • v.14 no.2
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    • pp.113-118
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    • 2007
  • A LED (light emitting diode) was attached inside the cold $(4^{\circ}C)$ compartment of a home refrigerator to investigate a possible effect on freshness prolongation of cabbage over 10 d of storage. The LED increased chlorophyll synthesis, inhibited vitamin C degradation and increased the content of phenolics, compared to values measured in cabbages stored in an identical refrigerator without the LED. In the refrigerator with the LED, the color of cabbage leaves remained green for a period fourfold longer than that seen in a conventional refrigerator. Vitamin C content was twice that of a conventionally stored cabbage, and phenolic consent was 14% higher in the LED-illuminated vegetables.

A Study on H-CNN Based Pedestrian Detection Using LGP-FL and Hippocampal Structure (LGP-FL과 해마 구조를 이용한 H-CNN 기반 보행자 검출에 대한 연구)

  • Park, Su-Bin;Kang, Dae-Seong
    • The Journal of Korean Institute of Information Technology
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    • v.16 no.12
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    • pp.75-83
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    • 2018
  • Recently, autonomous vehicles have been actively studied. Pedestrian detection and recognition technology is important in autonomous vehicles. Pedestrian detection using CNN(Convolutional Neural Netwrok), which is mainly used recently, generally shows good performance, but there is a performance degradation depending on the environment of the image. In this paper, we propose a pedestrian detection system applying long-term memory structure of hippocampal neural network based on CNN network with LGP-FL (Local Gradient Pattern-Feature Layer) added. First, change the input image to a size of $227{\times}227$. Then, the feature is extracted through a total of 5 layers of convolution layer. In the process, LGP-FL adds the LGP feature pattern and stores the high-frequency pattern in the long-term memory. In the detection process, it is possible to detect the pedestrian more accurately by detecting using the LGP feature pattern information robust to brightness and color change. A comparison of the existing methods and the proposed method confirmed the increase of detection rate of about 1~4%.

Recognition and Visualization of Crack on Concrete Wall using Deep Learning and Transfer Learning (딥러닝과 전이학습을 이용한 콘크리트 균열 인식 및 시각화)

  • Lee, Sang-Ik;Yang, Gyeong-Mo;Lee, Jemyung;Lee, Jong-Hyuk;Jeong, Yeong-Joon;Lee, Jun-Gu;Choi, Won
    • Journal of The Korean Society of Agricultural Engineers
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    • v.61 no.3
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    • pp.55-65
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    • 2019
  • Although crack on concrete exists from its early formation, crack requires attention as it affects stiffness of structure and can lead demolition of structure as it grows. Detecting cracks on concrete is needed to take action prior to performance degradation of structure, and deep learning can be utilized for it. In this study, transfer learning, one of the deep learning techniques, was used to detect the crack, as the amount of crack's image data was limited. Pre-trained Inception-v3 was applied as a base model for the transfer learning. Web scrapping was utilized to fetch images of concrete wall with or without crack from web. In the recognition of crack, image post-process including changing size or removing color were applied. In the visualization of crack, source images divided into 30px, 50px or 100px size were used as input data, and different numbers of input data per category were applied for each case. With the results of visualized crack image, false positive and false negative errors were examined. Highest accuracy for the recognizing crack was achieved when the source images were adjusted into 224px size under gray-scale. In visualization, the result using 50 data per category under 100px interval size showed the smallest error. With regard to the false positive error, the best result was obtained using 400 data per category, and regarding to the false negative error, the case using 50 data per category showed the best result.

Simple and Novel Assay of the Host-Guest Complexation of Homocysteine with Cucurbit[7]uril

  • Park, Se-Ho;Lee, Jae-Yeul;Cho, Hyun-Nam;Kim, Kyoung-Ran;Yang, Seun-Ah;Kim, Hee-Joon;Jhee, Kwang-Hwan
    • Journal of Microbiology and Biotechnology
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    • v.29 no.1
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    • pp.114-126
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    • 2019
  • This paper introduces three ways to determine host-guest complexation of cucurbit[7]uril (CB[7]) with homocysteine (Hcy). After preincubating Hcy and cysteine (Cys) with CB[7], Ellman's reagent (DTNB) was used to detect Hcy and Cys. Only Cys reacted with DTNB and Hcy gave a retarded color change. This suggests that the -SH group of Hcy is buried inside CB[7]. Human cystathionine ${\gamma}-lyase$ (hCGL) decreased the level of Hcy degradation after preincubating Hcy and CB[7]. These results suggest that the amount of free Hcy available was decreased by the formation of a Hcy-CB[7] complex. The immunological signal of anti-Hcy monoclonal antibody was decreased significantly by preincubating CB[7] with Hcy. The ELISA results also show that ethanethiol group ($-CH_2CH_2SH$) of Hcy, which is an epitope of anti-Hcy monoclonal antibody, was blocked by the cavity in CB[7]. Overall, CB[7] can act as a host by binding selectively with Hcy, but not Cys. The calculated half-complexation formation concentration of CB[7] was 58.2 nmol using Ellman's protocol, 97.9 nmol using hCGL assay and 87.7 nmol using monoclonal antibody. The differing binding abilities of Hcy and Cys towards the CB[7] host may offer a simple and useful method for determining the Hcy concentration in plasma or serum.