• Title/Summary/Keyword: Colony characteristics

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Effect of gacS and gacA Mutations on Colony Architecture, Surface Motility, Biofilm Formation and Chemical Toxicity in Pseudomonas sp. KL28

  • Choi, Kyung-Soon;Veeraragouda, Yaligara;Cho, Kyoung-Mi;Lee, Soo-O;Jo, Geuk-Rae;Cho, Kyung-Yun;Lee, Kyoung
    • Journal of Microbiology
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    • v.45 no.6
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    • pp.492-498
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    • 2007
  • GacS and GacA proteins form a two component signal transduction system in bacteria. Here, Tn5 transposon gacS and gacA (Gac) mutants of Pseudomonas sp. KL28, an alkylphenol degrader, were isolated by selecting for smooth colonies of strain KL28. The mutants exhibited reduced ability to migrate on a solid surface. This surface motility does not require the action of flagella unlike the well-studied swarming motility of other Pseudomonas sp. The Gac mutants also showed reduced levels of biofilm and pellicle formation in liquid culture. In addition, compared to the wild type KL28 strain, these mutants were more resistant to high concentrations of m-cresol but were more sensitive to $H_2O_2$, which are characteristics that they share with an rpoS mutant. These results indicate that the Gac regulatory cascade in strain KL28 positively controls wrinkling morphology, biofilm formation, surface translocation and $H_2O_2$ resistance, which are important traits for its capacity to survive in particular niches.

Knockdown of a Proliferation-inducing Ligand (PRIL) Suppresses the Proliferation of Gastric Cancer Cells

  • Cui, Jiu-Wei;Li, Yan;Wang, Chang;Yao, Cheng;Li, Wei
    • Asian Pacific Journal of Cancer Prevention
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    • v.13 no.2
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    • pp.633-636
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    • 2012
  • Purpose: PRIL (proliferation-inducing ligand) is a newly identified member of the tumor necrosis factor (TNF) family and modulates death ligand-induced apoptosis. Here, we investigated the effect of PRIL on cellular characteristics relating to tumor progression in human gastric cancer. Method: Recombinant lentivirus containing PRIL siRNA was constructed and then infected MGC803 and SGC7901 gastric cancer cells. MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] colony formation and cell cycle analysis were used to study the effect of PRIL knockdown on gastric cancer cell proliferation. Results: PRIL expression in lentivirus infected cells was significantly reduced as evidenced by quantitative real-time PCR. Cell viability and colony formation of MGC803 and SGC7901 cells were significantly hampered in PRIL knock-down cells. Moreover, the cell cycle was arrested at G2/M phase, elucidating the mechanism underlying the inhibitory effect of siRNA on cell proliferation. Conclusions: Our study indicated that PRIL functions in promoting cell growth, and lentivirus-mediated PRIL gene knockdown might be a promising strategy in the treatment of gastric cancer.

A Six-Phase CRIM Driving CVT using Blend Modified Recurrent Gegenbauer OPNN Control

  • Lin, Chih-Hong
    • Journal of Power Electronics
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    • v.16 no.4
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    • pp.1438-1454
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    • 2016
  • Because the nonlinear and time-varying characteristics of continuously variable transmission (CVT) systems driven by means of a six-phase copper rotor induction motor (CRIM) are unconscious, the control performance obtained for classical linear controllers is disappointing, when compared to more complex, nonlinear control methods. A blend modified recurrent Gegenbauer orthogonal polynomial neural network (OPNN) control system which has the online learning capability to come back to a nonlinear time-varying system, was complied to overcome difficulty in the design of a linear controller for six-phase CRIM driving CVT systems with lumped nonlinear load disturbances. The blend modified recurrent Gegenbauer OPNN control system can carry out examiner control, modified recurrent Gegenbauer OPNN control, and reimbursed control. Additionally, the adaptation law of the online parameters in the modified recurrent Gegenbauer OPNN is established on the Lyapunov stability theorem. The use of an amended artificial bee colony (ABC) optimization technique brought about two optimal learning rates for the parameters, which helped reform convergence. Finally, a comparison of the experimental results of the present study with those of previous studies demonstrates the high control performance of the proposed control scheme.

Colletotrichum Disease of Mungbean Sprout by Colletotrichum acutatum

  • Kim, Dong-Kil;Lee, Sun-Chul;Kang, Jin-Ho;Kim, Hee-Kyu
    • The Plant Pathology Journal
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    • v.19 no.4
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    • pp.203-204
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    • 2003
  • Decayed samples of marketed mungbean sprout were collected from Sacheon, Suncheon, and Dangjin cities in Korea. Initial symptom on hypocotyls was dark-brown diamond speck, which developed into enlarged sunken brownish-black spot with irregular margin, followed by softening yellowish decay. Brown speck on cotyledon further developed into irregular lesions. This study isolated the fungus Colletotrichum sp. The fungal colony was pale orange, which turned greenish gray after 1 week at $25^{\circ}C$. Colony of reverse side in a petri dish was pink. Neither conidiomata nor setae were present in the culture. Typical fusiform conidia sized 7.5-15.0$\times$2.5-2.9 $\mu\textrm$were hyaline, aseptate, smooth, and had salmon color in mass. Conidiogenous cells were phialidic, hyaline, smooth, and cylindrical with terminal distinct collarette. Basedon these mycological characteristics, the casual organism was identified as Colletotrichum acutatum. This is first report of Colletotrichum acutatum in Korea.

Characteristics of the Unidirectionally Solidified Al-Co Alloy (일방향응고시킨 Al-Co 합금의 특성에 관한 연구)

  • Park, Su-Jung;Jun, Hyun-Yong;Lee, Hyun-Kyu
    • Journal of Korea Foundry Society
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    • v.25 no.1
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    • pp.30-35
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    • 2005
  • The structures and mechanical property of the unidirectionally solidified Al-Co eutectic alloy were investigated. Al-Co eutectic alloy was unidirectionally solidified with growth rates(R) between I cm/hr and 10 cm/hr in the induction furnace maintaining the thermal gradient (G) at solid-liquid interface, $32^{\circ}C$/cm. The eutectic microstructure was varied with the growth condition(G/R ratio). When the G/R ratio was larger than $8.5{\times}10^{3}^{\circ}C/cm^{2}/sec$, a lamellar structure was formed, But the G/R ratio was smaller than $8.5{\times}10^{3}^{\circ}C/cm^{2}/sec$, a colony structure was formed. It was found that the interlamellar spacing(${\lambda}$) was dependent on the growth rate(R) with the relationship, ${\lambda}^{2}{\cdot}R=constant$. The microhardness of this eutectic alloy increased with increase in the growth rate.

Swarm Intelligence Based Data Dependant Routing Algorithm for Ad hoc Network (군집단 지능 알고리즘 기반의 정보 속성을 고려한 애드 혹 네트워크 라우팅)

  • Heo, Seon-Hoe;Chang, Hyeong-Soo
    • Journal of KIISE:Computing Practices and Letters
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    • v.14 no.5
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    • pp.462-466
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    • 2008
  • In this paper, we propose a Data Dependant Swarm Intelligence Routing Algorithm(DSRA) based on "ant colony optimization" to improve routing performance in Mobile Ad hoc Network(MANET). DSRA generates a different routing path depending on data's characteristics: Realtime and Non-Realtime. DSRA achieves a reduced delay for Realtime data and an enhanced network lifetime from a decentralized path selection for Non-Realtime data. We demonstrate these results by an experimental study comparing with AODV, DSR and AntHocNet.

Customer Behavior Pattern Discovery by Adaptive Clustering Based on Swarm Intelligence

  • Dai, Weihui
    • Journal of Information Technology Applications and Management
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    • v.17 no.1
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    • pp.127-139
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    • 2010
  • Customer behavior pattern discovery is the fundament for conducting customer oriented services and the services management. But, the composition, need, interest and experience of customers may be continuously changing, thereof lead to the difficulty in refining a stable description of their consistent behavior pattern. This paper presented a new method for the behavior pattern discovery from a changing collection of customers. It was originally inspired from the swarm intelligence of ant colony. By the adaptive clustering, some typical behavior patterns which reflect the characteristics of related customer clusters can extracted dynamically and adaptively.

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Cultural Characteristics of Mcyosphaerella nawae causing Spotted Leaf Casting of Persimmon (감나무 둥근무늬낙엽병균(Mycosphaerella nawae)의 배양적 특성)

  • 권진혁;강수웅;정부근;박창석
    • Plant Disease and Agriculture
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    • v.1 no.2
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    • pp.18-21
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    • 1995
  • Mycosphaerella nawae, the causal organism, of spotted leaf casing disease of persimmon, was isolated from infected leaves showing typical symptom. The cultural characteristics of the fungus were compared on artificial media. Among 24 different combinations of culture media and supplements, oatmeal agar+persimmon leaf extract (PLE) and PAD+ PLE+streptomycin showed the highest rate of isolating as 57.1%. The best medium for mycelial growth of the fungus was PDA+persimmon leaf powder (PLP). The colony diameter was reached 47mm for 30 days at 2$0^{\circ}C$. PDA+PLE also supported good mcyelial growth showing 46mm of diameter in same condition. The optimum growth temperature of this fungus in PDA was recognized fairly low. The mycelial growth was higher at 2$0^{\circ}C$ than 15$^{\circ}C$. The variation of pH between pH 4 to pH 8 did not affect to the mycelial growth of the pathogen.

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Quality Characteristics of Sulgidduk added with Rubus coreanum Miquel during Storage (복분자 첨가 설기떡의 저장 중 품질 특성)

  • Cho Eun-Ja;Yang Mi-Ok;Hwang Chi-Hui;Kim Woon-Jin;Kim Min-Jeong;Lee Mi-Kyung
    • Journal of the East Asian Society of Dietary Life
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    • v.16 no.4
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    • pp.458-467
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    • 2006
  • In order to develop a Bokbunja(Rubus coreanum Miquel) with natural & food applications, the effects of Bokbunja juice and wine on the physical, textural anti bacterial and sensory properties of sulgiduck were examined. Aw of Bokbunja sulgiduck(BS) decreased as storage time prolonged. Bokbunja juice sulgiduck(BJS) was higher than Bokbunja wine sulgiduck(BWS) in water activity. BJS and BWS showed low tendency in pH as additional level was high and storage time was prolonged. Hardness of the samples increased as the storage period increased, the samples exhibited a rapid increase after 24 hours of storage. BJS was lower significantly than those of none bokbunja after 6 hours of storage. Chewiness increased slowly to 12 hours. it appeared sudden decrease after 12 hours. Adhesiveness increase of the storage 24 hours it appeared. Springiness slowly reduced with the storage period, especially J4 was slowly reduced gumminess of the samples increased as the storage period. Cohesiveness increase of the slowly width for during the storage 12 hours, increase of the largest width was after save 12 hours. Color a and b values increased significantly, as storage time was prolonged. Total colony of all the BS increased. total colony of BWS decreased as additional level of Bokbunja wine. All the sensory characteristics indicated rapid decrease tendency after 12hr storage. BWS showed higher score than BJS in overall acceptability. BWS and BJS increased in color and flavor as additional level of juice and wine increased, while softness decreased as storage time was prolonged. BWS 3% showed highest score among all the BS through all storage time. BWS 3% was most excellent in sensory evaluation.

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Convergence Characteristics of Ant Colony Optimization with Selective Evaluation in Feature Selection (특징 선택에서 선택적 평가를 사용하는 개미 군집 최적화의 수렴 특성)

  • Lee, Jin-Seon;Oh, Il-Seok
    • The Journal of the Korea Contents Association
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    • v.11 no.10
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    • pp.41-48
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    • 2011
  • In feature selection, the selective evaluation scheme for Ant Colony Optimization(ACO) has recently been proposed, which reduces computational load by excluding unnecessary or less promising candidate solutions from the actual evaluation. Its superiority was supported by experimental results. However the experiment seems to be not statistically sufficient since it used only one dataset. The aim of this paper is to analyze convergence characteristics of the selective evaluation scheme and to make the conclusion more convincing. We chose three datasets related to handwriting, medical, and speech domains from UCI repository whose feature set size ranges from 256 to 617. For each of them, we executed 12 independent runs in order to obtain statistically stable data. Each run was given 72 hours to observe the long-time convergence. Based on analysis of experimental data, we describe a reason for the superiority and where the scheme can be applied.