• 제목/요약/키워드: Collybia confluens

검색결과 9건 처리시간 0.026초

Hypoglycemic Effect of Collybia confluens Exobiopolymer Produced by Submerged Mycelial Culture on Diabetic Rats

  • YANG, BYUNG-KEUN;LEE, HYUN-JI;JEONG, SANG-CHUL;LIM, WANG-JIN;SONG, CHI-HYUN
    • Journal of Microbiology and Biotechnology
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    • 제15권1호
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    • pp.136-140
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    • 2005
  • The hypoglycemic effect of Collybia confluens exobiopolymer was investigated in streptozotocin (STZ)-induced diabetic rats. In a dose-dependent study, the exobiopolymer, at 150 mg/kg body weight (BW) dose substantially lowered the plasma glucose level by 29.3%, as compared to the control group. It also lowered the plasma total cholesterol and triglyceride levels by 23.3 and 30.7%, respectively, and reduced the liver total cholesterol and triglyceride levels by 23.0 and 33.5%, respectively. The activity of alanine transaminase (ALT) and aspartate transaminase (AST) was reduced 34.6% and 23.6% respectively, by the exobiopolymer administration, compared to the control group. The exobiopolymer was found to contain 83.2% carbohydrate and 16.8% protein. The sugar and amino acid of the exobiopolymer were also analyzed in detail.

Antidiabetic and Hypolipidemic Effects of Collybia confluens Mycelia Produced by Submerged Culture in Streptozotocin-Diabetic Rats

  • Yang, Byung-Keun;Jeong, Sang-Chul;Lee, Hyun-Ji;Sohn, Dong-Hwan;Song, Chi-Hyun
    • Archives of Pharmacal Research
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    • 제29권1호
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    • pp.73-79
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    • 2006
  • This investigation was undertaken to study the effects of oral administration (3 weeks) of Collybia confluens mycelial powder (CCMP) produced by a submerged culture on plasma glucose and other biochemical parameters in streptozotocin (STZ)-induced diabetic rats. Antidiabetic and hypolipidemic effects were proportionally increased with the increasing concentration of the CCMP for oral administration. The CCMP, at the dose of 400 mg/kg BW, substantially reduced the plasma glucose level by as much as 33.1 % as compared to the STZ-induced diabetic rats group. It also lowered the plasma total cholesterol, triglyceride, and low density lipoprotein (LDL) cholesterol by 22.9%, 19.9%, and 37.3%, respectively. The levels of total cholesterol and triglyceride in liver were reduced to the extent of by 13.5% and 18.8%, and the activity of alanine transaminase (ALT) and aspartate transaminase (AST) was decreased by 48.8% and 37.2%, respectively, under the influence of CCMP. The general components of CCMP were found to contain 26.18% carbohydrate, 3.67% crude ash, 4.02% crude fat, 22.55% crude protein, and 43.58% dietary fiber. The amino acid composition of the CCMP was also analyzed in detail.

Anti-tumor Effects of Exo- and Endo-biopolymers Produced from Submerged Cultures of Three Different Mushrooms

  • Jeong, Yong-Tae;Yang, Byung-Keun;Li, Chun-Ru;Song, Chi-Hyun
    • Mycobiology
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    • 제36권2호
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    • pp.106-109
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    • 2008
  • The anti-tumor effects of exo- (EX) and endo-biopolymers (EN) produced from submerged mycelial cultures of Ganoderma applanatum (GA), Collybia confluens (CC), and Pleurotus eryngii (PE) were studied using Sarcoma 180 bearing mice. Solid tumor growth was inhibited most effectively when 40 mg/kg body weight (BW) of GA-EX or PE-EN was administered to the intraperitoneal (i.p.) cavity of BALB/c mice. The spleen and liver indexes were increased in mice following i.p. administration of GA-EX and PE-EN fractions. GA-EX and PE-EN reduced the tumor formation by 30.7% and 29.4%, respectively. GA-EX and PE-EN increased the natural killer (NK) cell activity of splenocytes by 41.3% and 28.9%, respectively.

밀버섯의 항암성분에 관한 연구 (Studies on Antitumor Components of Collybia confluens)

  • 김숙희;김진숙;진미림;김하원;최응칠;김병각
    • 생약학회지
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    • 제24권4호
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    • pp.267-281
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    • 1993
  • To find antitumor components from higher fungi, the mycelia of Collybia confluens (Pers. ex Fr.) Kummer were cultured in artificial media. For efficient production of the mycelia, the influences of various modifications of culture conditions were examined. A water-soluble protein-bound polysaccharide fraction, Fr. A, was obtained from the mycelia by hot water extraction. When Fr. A was purified and fractionated by DEAE-cellulose and Sepbadex G-200 gel filtration chromatographies into four fractions which were designated B, C, C-I and C-II. The tumor inhibition ratios of these fractions ranged from 46% to 75% against the solid forms of sarcoma 180 in ICR mice at doses of 20 and 50 mg/kg/day when given intraperitoneally. Especially, Fr. C which was named Collyban(CB) exhibited a marked life-prolonging effect of the mice against ascitic forms of sarcoma 180 at a dose of 50 mg via i.p. administration. To extend spectra of the antitumor activities and eliminate the effects of allograft rejection, the characterization of antitumor effects of CB was performed in syngeneic host-tumor systems. It did not show any antitumor activity against L1210 murine leukemia in $CD_2Fl$ mice but prolonged their life span against ascitic forms of $MM_{46}$ carcinoma in $C_3H/He$ mice. Also it exhibited antitumor activity against human cervical cancer HeLa cells that were xenografted into nude mice having BALB/c genetic backgrounds by the i.p. injection at a dose of 100 mg/kg/day. In order to characterize the antitumor components, CB was examined by chemical analysis. It was acidic protein-bound polysaccharides composed of 31% polysaccharide, 27% protein and 3% hexosamine. CB was fractionated into two fractions, Fr. C-I(M.W.: 500 Kd) and Fr. C-II(M.W.:30 and 8 Kd) by Sephadex G-200 gel filtration chromatography.

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한국산 민자주방망이 버섯의 유전적 변이 (Genetic Variation of Korean Lepista nuda)

  • 김승희;김종봉
    • 생명과학회지
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    • 제14권1호
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    • pp.115-120
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    • 2004
  • 민자주방망이버섯은 우수한 식용버섯으로 세계적으로 분포하고 있는 종이다. 민자주방망이버섯과 5가지 송이과버섯 종들의 유전적인 변이와 분류학적 관계를 RAPD에 의해 분석 하였다. RAPD에 15가지 random primer를 사용하였다. 버섯들의 유전적 거리는 UPCMA를 사용하여 측정하였고 계통유전학적 유연관계는 neighnor-joining (NJ) 방법을 사용하여 분석하였다. PCR에 의하여 증폭된 RAPD 절편들은 100bp에서 1600 bp이었다. Nei-Li의 유전적 거리는 228개의 DNA밴드를 사용하여 계산하였고 이를 바탕으로 계통유전학적 계통수를 만들었다. 민자주방망이버섯, 자주방망이버섯 아재비, 가랑잎애기버섯, 밀버섯, 만가닥버섯, 졸각버섯의 유전적 변이 는 각각 0∼21.3%, 21.2∼28.0%, 15.4∼23.0%, 14.0∼21.8%, 16,5∼34.6%, 12.4∼27.4%이였다. CI (Consistency index), RI (Retention index), HI (Homoplasy inedx)의 값은 각각 0.5217, 0.5769, 0.5156이었다. 또한 NJ tree로 두 그룹을 만들 수 있었다. 유전적 거리는 민자주방망이버섯과 자주방망이버섯아재비보다 민자주방망이버섯과 밀버섯이 더 가까웠다.

대둔산 도립공원의 균류다양성과 균류자원 (Biodiversities and Fungal Resources of Mt.Daedun Provincial Park)

  • Cho, Duck-Hyun;Kim, Jong-Moon
    • 한국자원식물학회:학술대회논문집
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    • 한국자원식물학회 2003년도 춘계 학술발표대회
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    • pp.119-120
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    • 2003
  • Many fungi were collected at Mt. Daedun Provincial Park between the periods from March 2001 to October 2002 and they were identified. According to the results, the fungi diversity included 2 division, 3 subdivisions, 5 classes, 4 subclasses, 12 orders, 35 families, 75 genera and 153 species. Among them Ileodictyon is unrecorded genus and Ileodictyon gracile, Mycena flosum-nivium and Tricholoma lascivum are newly to Korea. Dominant family are Tricholomataceae, Russulaceae and Polyporaceae;genus Amanita and species Collybia confluens and Russula emetica In useful fungi and ecological resources, edible fungi included 51 species; culture fungi 10; poisonous fungi 18: medicinal fungi 17: anticancer fungi 22: ectomycorrhizal fungi 26 and wood rotting fungi 51, In geographical distributions of Chonbuk areas, same species are 50 spcies of Jeongup ; 56 of Mt.Manduck : 84 of Mt.Jiri : 28 of Pyonsan national park : 59 of Cheonju arboretum and 51 of Mt,Sunun.

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중금속 오염지 및 건전지역에서 수집된 담자균류의 분포 (Population and transfer factor of basidiomycota collected in the heavy metal-contaminated and healthy soils)

  • 장갑열;최선규;이강효;석순자;정종천;정구복;김규현;성재모
    • 한국버섯학회지
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    • 제3권4호
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    • pp.145-153
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    • 2005
  • 미생물을 이용한 중금속 오염지의 생물적 개량연구를 위하여 구리, 납 등 중금속 오염지인 은성 폐광과 도곡 폐광지역에서 야생버섯을 수집 동정하고 중금속 함량을 분석한 결과 Laccaria laccata가 가장 높게 나타났으며 토양 내 중금속함량이 높을수록 버섯 내 중금속 농도도 높았다. 그러나, 전이계수를 이용하여 버섯 자실체의 중금속 이행률을 알아본 결과, Amanita volvata가 카드뮴과 비소의 이행률이 가장 높았으며, 구리의 이행률은 Agaricus praesquamosus가 다른 버섯에 비해 월등히 높은 수치를 나타냈다. 납은 Mycena pura, 아연은 Marasmius pulcherripes, 니켈은 Laccaria laccata, 크롬은 Collybia confluens로 나타났다. 중금속 오염지와 비오염지인 치악산 지역에서의 담자균의 발생 분포를 비교한 결과, Russula와 Collybia 속은 비오염지와 오염지역 모두에서 발생되었으며 Leucocoprinus, Coprinus, Suillus, Lepiost, Gyroporus. Lepista, Microstoma, Stropharia, Agrocybe 속은 오염지에서만 발생되었다. 이상의 결과, Laccaria laccata 등 일부 담자균은 중금속 오염지 저감에 이용할 가능성이 있으며 이를 위한 많은 연구가 기대된다.

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대성산(大成山)과 대덕산(大德山) 지역(地域)의 고등균류(高等菌類) (The Higher Fungal Flora in the Areas of Mt. Daesung and Mt. Daeduck)

  • 김경숙;박완희;민경희
    • 한국균학회지
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    • 제19권3호
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    • pp.167-174
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    • 1991
  • 1990년(年) 8월(月) 13일(日) 부터 19일(日)까지 강원도(江原道) 대덕산(大德山)과 대성산일대(大成山一帶)에서 자생(自生)하는 한국산(韓國産) 고등균류(高等菌類) 314 표본(標本) 을 채집(採集)하여 동정(同定)한 결과(結果) Basidiomycotina 에 33속(屬) 49종(種) 1변종(變種), Ascomycotina에 2속(屬) 3종(種)을 분류(分類)하였 으며 한국산(韓國産) 미기록종(未記錄種)으로 확인(確認)한 것은 다음과 같으며 이들에 대하여 보통명(普通名)을 신칭(新稱)하였다 ; Phlogiotis hevelloides (Fr.) Pseudohydnum gelatinosum.

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리기다소나무림의 버섯의 다양성, 생산량 및 영양염류 함량 (Diversity, Production and Nutrients Concentration of Mushrooms in a Pitch Pine Forest)

  • 남궁정;문형태
    • 환경생물
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    • 제25권3호
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    • pp.197-204
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    • 2007
  • 2000년 3월부터 2000년 11월까지 공주시 근교의 리기다소나무림에서 계절별 버섯의 출현종과 종별 개체수, 생산량 및 영양염류 함량을 조사하였다. 조사기간 중 영구방형구 내에 출현한 종은 총 32종류이었다. 버섯은 6월부터 출현하기 시작하여 9월에 종다양성과 생산량이 가장 높았다. 월별 우점종은 6월과 7월에는 밀버섯, 8월과 9월은 갈색털꾀꼬리버섯 이었으며, 10월에 젖버섯아재비버섯을 끝으로 더 이상 출현하지 않았다. 연중 가장 많은 개체수를 보인 버섯은 갈색털꾀꼬리버섯이었다. 버섯의 연간 생산량은 12.13kg $Dwha^{-1}yr^{-1}$으로 조사되었으며, 8월과 9월에 각각 3.95, 6.92kg $Dwha^{-1}$로 전체 생산량의 95%이상을 차지하였다. 월별 생산량이 많은 종은 7월에는 밀버섯, 8월과 9월에는 갈색털꾀꼬리버섯이었으며, 최대 생산량을 보인 버섯은 갈색털꾀꼬리버섯으로 전체 생산량의 27.1%를 차지하였다. 버섯의 평균 영양염류 함량은 때, N, P, K, Ca 그리고 Mg가 각각$38.81{\pm}4.82,\;0.90{\pm}0.44,\;28.41{\pm}7.81,\;0.24{\pm}0.16,\;0.76{\pm}0.26mg\;g^{-1}$으로 Ca과 Mg을 제외하고는 주변의 낙엽이나 토양보다 훨씬 높은 값이었다. 버섯을 통해 연간 임상에 축적되는 영양염류의 양은 N, P, K, Ca그리고 Mg가 각각 463.41, 10.26, 345.21, 3.14, $8.99g\;ha^{-1}yr^{-1}$이었다.