• 식물병연구
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• 제26권3호
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• pp.190-193
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• 2020

강원도 강릉시 사천면 비닐하우스에서 재배중인 호박 과실에 탄저병이 발생하였다. 병든 호박 과실에 분홍색의 분생포자 층이 동심원으로 나타나 점차 확대되어 과실이 무르는 증상을 나타내었다. 원인균을 규명하기 위하여 순수 분리 후 균학적 특성 및 ITS, GAPDH, CHS-1, HIS3, ACT, TUB2 염기서열 분석결과 Colletotrichum fioriniae로 동정하였다. 또한, 병원성이 확인되었고 접종시험에서 동일한 균이 반복적으로 분리되었다. 따라서, 이러한 결과를 바탕으로 C. fioriniae에 의한 호박 과실에 발생하는 탄저병의 발생을 국내 처음으로 보고한다.

• Mycobiology
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• 제44권4호
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• pp.325-329
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• 2016

A fungus, Colletotrichum fioriniae, was isolated for the first time from fruits of Chinese matrimony vine (Lycium chinense Mill.) in Korea. It was classified as C. fioriniae based on the morphological characteristics and nucleotide sequence of glyceraldehyde-3-phosphate-dehydrogenase and ${\beta}-tubulin$. To the best of our knowledge, this is the first report of C. fioriniae causing anthracnose of Chinese matrimony vine in Korea.

• 식물병연구
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• 제29권4호
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• pp.345-362
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• 2023

탄저병원균(Colletotrichum) 속의 많은 식물병원균은 한국을 비롯하여 전 세계 과수 생산에 큰 위협이 된다. 사과, 감, 자두, 복숭아, 대추, 포도 및 호두와 같은 과실에서 탄저병과 관련된 탄저병원균의 종이 동정되었다. 형태적, 다중 유전자의 계통발생적 및 병원성 시험이 다양한 접근으로 실시되었다. 동정된 탄저병원균의 종에 대한 감수성은 살균제에 대하여 평가하였다. 2종의 종복합체인 gloeosporioides와 acutatum에 속한 9종의 탄저병원균이 국내 과실에 탄저병의 주요 원인균으로 동정되었다. Colletotrichum gloeosporioides 종 복합체 안에는 6종인 C. aenigma, C. fructicola, C. gloeosporioides, C. horii, C. siamense 및 C. viniferum이 있는 반면, Colletotrichum acutatum 종 복합체 안에 종은 C. fioriniae, C. nymphaeae 및 C. orientalis이 동정되었다. 사과 탄저병원균은 C. fructicola, C. siamense, C. fioriniae 및 C. nymphaeae, 자두 탄저병원균은 C. siamense, C. fioriniae 및 C. nymphaeae, 복숭아 탄저병원균은 C. siamense, C. fructicola 및 C. fioriniae, 감 탄저병원균은 C. siamense, C. horii 및 C. nymphaeae, 오미자 탄저병원균은 C. fioriniae, 호두탄저병원균은 C. orientalis, 대추탄저병원균은 C. nymphaeae, 포도 탄저병원균인 C. aenigma, C. fructicola 및 C. siamense를 국내 과수류의 과실에 병을 일으키는 병원균의 종류로 처음 국제학술지에 보고되었다. 이들 병원균의 종에 대한 살균제의 감수성 시험에서 여러 살균제에 대한 탄저병원균의 종 간에 EC₅₀값이 매우 다양하여 감수성의 차이가 크게 나타났다.

• The Plant Pathology Journal
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• 제34권6호
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• pp.480-489
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• 2018

Bitter rot caused by Colletotrichum species is a common fruit rotting disease of apple and one of the economically important disease in worldwide. In 2015 and 2016, distinct symptoms of bitter rot disease were observed in apple orchards in five regions of South Korea. In the present study, infected apples from these regions were utilized to obtain eighteen isolates of Colletotrichum spp. These isolates were identified and characterized according to their morphological characteristics and nucleotide sequence data of internal transcribed spacer regions and glyceraldehyde-3-phosphate-dehydrogenase. Molecular analyses suggested that the isolates of Colletotrichum causing the bitter rot disease in South Korea belong to 4 species: C. siamense; C. fructicola; C. fioriniae and C. nymphaeae. C. siamense and C. fructicola belonged to Musae Clade of C. gloeosporioides complex species while C. fioriniae and C. nymphaeae belonged to the Clade 3 and Clade 2 of C. acutatum complex species, respectively. Additionally, we also found that the isolates of C. gloeosporioides species-complex were more aggressive than those in the C. acutatum species complex via pathogenicity tests. Taken together, our results suggest that accurate identification of Colletotrichum spp. within each species complex is required for management of bitter rot disease on apple fruit in South Korea.

• Journal of Forest and Environmental Science
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• 제37권2호
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• pp.154-162
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• 2021

Blueberry, which produces phenolic compounds, is one of the most popular fruits in Korea. During a survey on blueberry diseases, 16 Colletotrichum isolates were obtained from blueberry leaves in Chuncheon and Gosung, Kangwon province, Korea. Using morphological and molecular analyses, the isolates were identified as Colletotrichum aenigma, C. fioriniae, C. fructicola, C. gloeosporioides, and C. nymphaeae. C. gloeosporioides was the most frequently isolated (11 isolates), and only one or two isolates of the other species were found. After inoculation with all isolates, those leaves and fruits with wounds easily developed anthracnose; whereas, fruits without wounds became infected but leaves without wounds were infected by only two of C. gloeosporioides. Typically, around seven fungicidal agents are used to control anthracnose on blueberries in Korea. Fluzinam and prochloraz manganese complex strongly (over than 80%) inhibited the growth of all Colletotrichum species, while dithianon and mancozeb only weakly (about 80% or less) inhibited their growth.

• Mycobiology
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• 제48권3호
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• pp.210-218
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• 2020

Peach (Prunus persica L.) is one of the major fruit crops in South Korea, along with apple, persimmon, and Asian pears. Peach anthracnose is a continuing threat to growers and is accountable for enormous economic loss. In July 2018, anthracnose of peach appeared at different peach orchards in Gyeongsangbuk-do region, Korea. The typical anthracnose symptoms (brown, circular, and necrotic lesions) were observed on the fruits. Anthracnose of peach was surveyed in different peach orchards of Gyeongsangbuk-do, and 20 fungal isolates from 19 diseased fruits were collected. Multigene phylogenetic analyses coupled with morphological characteristic analysis approaches were used for identifying the fungal species isolated from diseased fruits. This study confirmed three Colletotrichum species. Based on the results, Colletotrichum siamense are reported for the first time as causal agents of peach anthracnose alongside C. fructicola and C. fioriniae, which has been reported previously. Pathogenicity assays were performed for the three isolates representing all the species identified, and Koch's postulates on detached healthy peach fruits were verified. All the identified species were pathogenic on peach fruits as the typical anthracnose symptoms were reproduced. Significant variations in the virulence were observed among fungal species on peach fruit.

• The Plant Pathology Journal
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• 제39권4호
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• pp.384-396
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• 2023

Colletotrichum acutatum species complex is one of the most important groups in the genus Colletotrichum with a high species diversity and a wide range of host plants. C. acutatum and related species have been collected from different plants and locations in Korea and deposited into the Korean Agricultural Culture Collection (KACC), National Institute of Agricultural Sciences since the 1990s. These fungal isolates were previously identified based mainly on morphological characteristics, and a limitation of molecular data was provided. To confirm the identification of species, 64 C. acutatum species complex isolates in KACC were used in this study for DNA sequence analyses of six loci: nuclear ribosomal internal transcribed spacers (ITS), betatubulin 2 (TUB2), histone-3 (HIS3), glyceraldehyde3-phosphate dehydrogenase (GAPDH), chitin synthase 1 (CHS-1), and actin (ACT). The molecular analysis revealed that they were identified in six different species of C. fioriniae (24 isolates), C. nymphaeae (21 isolates), C. scovillei (12 isolates), C. chrysanthemi (three isolates), C. lupini (two isolates), and C. godetiae (one isolate), and a novel species candidate. We compared the hosts of KACC isolates with "The List of Plant Diseases in Korea", previous reports in Korea and global reports and found that 23 combinations between hosts and pathogens could be newly reported in Korea after pathogenicity tests, and 12 of these have not been recorded in the world.