• 식물병연구
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• 제14권3호
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• pp.219-222
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• 2008

C. gloeosporioides와 C. acutatum의 개체군 밀도분석을 위해 기존 ITS부위를 이용한 PCR방법에 사용한 caInt2와 cgint 프라이머에 형광을 표지하여 C. acutatum에 특이적인 fcaInt2와 C. gloeosporioides에 특이적인 vcgint의 두 probe를 제작하였다. 이 두개의 프라이머와 Unicof1, Unicor1 primer를 이용 real time PCR을 수행하였을 때 C. acutatum은 fcaInt2 probe에, C. gloeosporioides는 vcgint에 특이적인 형광증폭곡선을 나타냄에 따라 delta Rn 값을 비교함으로 두 종의 구분이 가능하였다.

• 한국식물병리학회지
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• 제10권2호
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• pp.105-111
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• 1994

Pathogenic fungal species associated with apple anthracnose were studied with diseased fruits collected over two major apple growing areas from 1986 to 1988. Colletotrichum acutatum Simmonds, c. gloeosporioides(Penz.) Sacc. and Glomerella cingulata(Stonem.) Spauld. & Schrenk were identified based on their mycological characteristics. C. acutatum could be easily differentiated from C. gloeosporioides by producing their fusiform conidia and developing apricot∼salmon colony colors, non-forming an ascigerous stages. Growth rate of C. acutatum were slow in plate culture. The three species of anthracnose fungi tested caused apple rot. Symptoms produced by C. gloeosporioides were larger than those of the others. C. gloeosporioides and G. cingulata produced rot lesions on apples that appeared became brown and later black, but C. acutatum produced rot lesions appeared yellowish brown and later reddish brown. G. cingulata has the major species responsible for apple anthracnose, epidemics in Korea.

• Mycobiology
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• 제35권4호
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• pp.238-240
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• 2007

Anthracnose symptoms often occurred on fruits of Asian pear trees grown in Anseong, Naju, Seonghwan and Pyeongtaek in Korea during the harvesting period from 2000 to 2005. A total of 28 isolates of Colletotrichum sp. were obtained from the anthracnose symptoms. All the isolates were identified as Colletotrichum acutatum based on their morphological and cultural characteristics. Four isolates of the fungus were tested for pathogenicity to fruits of Asian pear tree by artificial inoculation. All the isolates induced anthracnose symptoms on the fruits by wound inoculation but not by unwound inoculation. The anthracnose symptoms induced by artificial inoculation were similar to those observed in the orchard. This is the first report of anthracnose of Asian pear tree caused by Colletotrichum acutatum.

• Mycobiology
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• 제37권4호
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• pp.310-312
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• 2009

A total of 82 isolates of Colletotrichum species were obtained from anthracnose symptoms of highbush blueberry trees grown in the Gochang area of Korea during a disease survey in 2008. Out of the isolates, 75 were identified as Colletotrichum gloeosporioides and the others as C. acutatum based on their morphological and cultural characteristics. Twenty six of C. gloeosporioides isolates produced their teleomorph Glomerella cingulata in PDA culture. Three isolates of each C. gloeosporioides and C. acutatum caused anthracnose symptoms on the leaves by artificial inoculation, which were similar to what was observed in the orchards. Previously in Korea, only C. gloeosporioides has been reported as causing anthracnose in blueberries. This is the first report that C. acutatum causes anthracnose in the highbush blueberry in Korea.

• 식물병연구
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• 제10권3호
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• pp.203-208
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• 2004

붉은 고추 탄저병균의 우점종인 Colletotrichum gloeosporioides는 C. acutatum에 비해 병원성이 강하였다. C. gloeosporioides는 G와 R계통이 존재하였으며, G계통이 R계통보다 더욱 강한 병원성을 보였다. 붉은 고추 품종간의 병원성 검정 결과, 금세기 품종이 가장 감수성이었고, 파페트 품종이 가장 약한 감수성이었다. 참깨, 홍화, 마, 그리고 딸기와 같은 다른 기주에서 분리되어진 C. gloeosporioides는 붉은 고추에 감염을 일으켰다.

• 식물병연구
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• 제13권2호
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• pp.119-121
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• 2007

2004년부터 2005년까지 2년간 경남농업기술원 약초재배 포장에 재배중인 배암차즈기에서 이상증상이 발생하였다. 병징은 잎, 잎자루, 줄기 등 식물체 전부위에서 발생하였다. 식물체가 서서히 시들기 시작하면서 수침상으로 물러지고 썩으면서 갈색 또는 암갈색으로 변색되어 결국 말라 죽는다. 균총의 색깔은 감자한천배지에서 회색이며 배지표면에 분홍색의 분생포자퇴를 형성하였다. 분생포자의 모양은 단세포, 방추형이며, 크기는 $8{\sim}17{\times}3{\sim}4\;{\mu}m$이었다. 부착기는 연한 암갈색이고 곤봉상이며 크기는 $8{\sim}11{\times}4{\sim}6\;{\mu}m$이었다. 균사생육 적온은 $25^{\circ}C$였다. 이상의 결과로 보아, 이 병원균을 Colletotrichum acutatum J. H. Simmonds에 의한 배암차즈기 탄저병으로 명명할 것을 제안한다.

• 식물병연구
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• 제12권2호
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• pp.119-121
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• 2006

2004년부터 2005년까지 경남농업기술원 약초연구팀과 합천군 가회면 농가포장에서 석류나무 과실이 심하게 부패되는 증상이 발생하였다. 병징은 과실의 끝부분이 갈색 또는 암갈색의 수침상으로 썩는다. 균총의 색깔은 감자한 천배지에서 회색이며 배지표면에 분홍색의 분생포자퇴를 형성하였다. 분생포자의 모양은 단세포, 방추형이며, 크기는 $8{\sim}16{\times}3{\sim}4{\mu}m$ 이었다. 부착기는 연한 암갈색이고 곤봉 상이며 크기는 $8{\sim}12{\times}4{\sim}6{\mu}m$ 이었다. 균사생육 적온은 $25^{\circ}C$였으며 유상접종에서 병원성이 잘 발현되었다. 이상의 결과로 보아, 이 병원균을 Colletotrichum acutatum에 의한 석류나무 탄저병으로 명명할 것을 제안한다.

• The Plant Pathology Journal
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• 제23권2호
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• pp.37-44
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• 2007

Colletotrichum contains many important pathogens which cause economically significant diseases of crops like pepper, strawberry, tomato and apple. Forty four isolates were collected to characterize the diversity of Colletotrichum causing apple anthracnose in various regions of Korea. They were analyzed by random amplified polymorphic DNA (RAPD), internal transcribed spacer (ITS) of rDNA and partial $\beta$-tubulin gene DNA sequence, and culture characteristics on PDA and PDA-Benomyl. From the results of molecular analyses, 31 strains belonged to Colletotrichum gloeosporioides, ribosomal DNA group (RG) 4 of Moriwaki et al. (2002), 8 strains belonged to C. acutatum, A2 group of Talhinhas et al. (2005) and 5 strains to C. acutatum, A3 group of Talhinhas et al. (2005). Most isolates of C. gloeosporioides RG4 grew faster on PDA than strains of C. acutatum, A2 and A3 groups and most RG4 strains were sensitive to Benomyl. However, a few strains of RG4 grew slower and were resistant to Benomyl. On the basis of molecular characteristics, apple isolates of C. acutatum were clearly differentiated from red pepper isolates of the species, but apple isolates of C. gloeosporioides were not.

• The Plant Pathology Journal
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• 제24권1호
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• pp.80-83
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• 2008

Anthracnose symptoms were frequently observed on fruits of peach trees grown in Yeongi in Korea during disease survey in August from 2000 to 2005. The disease incidence was as high as 40% at its maximum in the orchards investigated. A total of 24 isolates of Colletotrichum species were obtained from the anthracnose symptoms, out of which 20 were identified as Colletotrichum gloeosporioides and the four remaining ones as C. acutatum based on their morphological and cultural characteristics. Two isolates of each C. gloeosporioides and C. acutatum caused anthracnose symptoms on the fruits by both wound and unwound inoculation, which were similar to those observed in the orchard. The symptoms appeared more rapidly by the wound inoculation than by the unwound inoculation. There was no difference in pathogenicity between the C. gloeosporioides and C. acutatum isolates tested. In Korea, only C. gloeosporioides has been recorded as the causal fungus of anthracnose of peach tree. This is the first report that C. acutatum also causes anthracnose of peach tree in Korea.

• The Plant Pathology Journal
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• 제15권3호
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• pp.172-174
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• 1999

Anthracnose of cosmos caused by Colletotrichum acutatum is reported for the first time in Korea. The disease occurred widely in Kyeongnam province in1998 and the symptoms of sunken brownish spots were severely developed on stems. The fungi showed strong pathogenicity to the plant and are required at least 3 hr for conidial germination and host infection.