• Title/Summary/Keyword: Collection method

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Genome-based identification of strain KCOM 1265 isolated from subgingival plaque at the species level

  • Park, Soon-Nang;Lim, Yun Kyong;Kook, Joong-Ki
    • International Journal of Oral Biology
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    • 제45권2호
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    • pp.70-75
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    • 2020
  • The aim of this study was to identify strain KCOM 1265 isolated from subgingival plaque at the species level by comparing 16S ribosomal RNA gene (16S rDNA) and genome sequences. The whole genome of strain KCOM 1265 was extracted using the phenol-chloroform extraction method. 16S rDNA was amplified using polymerase chain reaction and sequenced using the dideoxy chain termination method. Pairwise genome comparison was performed using average nucleotide identity (ANI) and genome-to-genome distance (GGD) analyses. The data showed that the percent similarity of 16S rDNA sequence of strain KCOM 1265 was 99.6% as compared with those of Fusobacterium polymorphum ATCC 10953T and Fusobacterium hwasookii KCOM 1249T. The ANI values of strain KCOM 1265 with F. polymorphum ATCC 10953T and F. hwasookii KCOM 1249T were 95.8% and 93.0%, respectively. The GGD values of strain KCOM 1265 with F. polymorphum ATCC 10953T and F. hwasookii KCOM 1249T were 63.9% and 49.6%, respectively. These results indicate that strain KCOM 1265 belongs to F. polymorphum.

내러티브 탐구를 통한 일 대학 간호학생들의 보건소실습 경험 연구 (A Study on Nursing Students' Experience during Clinical Practice at a Public Health Center)

  • 최혜정
    • 한국보건간호학회지
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    • 제19권2호
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    • pp.217-228
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    • 2005
  • Purpose: The purpose of this study is to understand nursing students' experiences during clinical practice at a public health center. Method: This research used narrative inquiry far data collection. From April 2005 to June 2006, data collection was conducted by open-ended interview, questionnaire and close observation. The participants, who were student nurses, were willing to take part in this study. Results: On the basis of these data, the experiences of clinical practice at public health center were: 1) when the student nurses begin clinical practice at public health centers for the first time, most of the students feel fearful, nervous and stressed. They also mentioned having a hard time being polite to clients and the staff. 2) The students had new experiences at the health public center compared with clinical practice. Especially, the student nurses who were determined to be good nurses were doing home visiting care service. Not only did they have the opportunity to confirm their identity as nurses, but also the students change their career course from clinical nursing to public health nursing. 4) They reflected on themselves after home visiting care service. Conclusion: On the basis of these findings, the following recommendations are made. 1) Data collection and analysis are needed, net only through the narrative method, but also through other various qualitative methods. 2) Comparative study is necessary to enhance clinical experiences through the analysis of the interfering factors and the original experiences.

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접합분리 시험체를 사용한 구조체 콘크리트 강도 평가에 관한 실험적 연구 (Studies on the evaluation method of structural concrete strength using joint separation test body)

  • 김성덕;이선호;김광기;정광식;임남기;정상진
    • 한국콘크리트학회:학술대회논문집
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    • 한국콘크리트학회 2008년도 춘계 학술발표회 제20권1호
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    • pp.993-996
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    • 2008
  • 현재 건설현장에서 구조체 콘크리트 강도는 구조체에서 채취한 코어공시체 강도가 가장 신뢰성이 높다고 알려져 있다. 그러나 코어 채취시 구조체의 손상이나 배근의 절단에 의해서 구조체 성능의 지장을 줄 우려가 있고 공시체 채취후의 보수 등에 몇 가지 문제가 있다. 또한 비파괴 검사에 의한 경우 결과값을 추정하는 방법이기 때문에 신뢰도가 매우 저하되는 단점을 가지고 있다. 본 연구 개발의 목표는 접합 분리 시험체 및 콘크리트의 코어채취에 의한 직접적인 강도 측정방법과 계절별 관리용 공시체 강도를 비교분석하여 보다 발전된 구조체 콘크리트의 성능 평가방법을 제시하여 합리적이고 실용적인 구조체 콘크리트의 시공 품질관리 기법을 제안 하는데 목적이 있다.

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A Detection Kit for Aeromonas hydrophila Using Antibody Sensitized Latex

  • Shin, En-Joo;Lee, Soon-Deuk;Lee, Kyung-Won;Lee, Yeon-Hee
    • Journal of Microbiology and Biotechnology
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    • 제10권5호
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    • pp.595-598
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    • 2000
  • Aeromonas hydrophila is a pathogen to fish as well as human. It is a food-borne disease, and causes severe mortality in fish, and sometimes severe septicemia in human. In this study, a rapid detection method using latex agglutination has been developed for A. hydrophila. Polyclonal antibodies were raised against membrane and whole cells of three isolates from rainbow trout. Among these, latex particles coated with antibodies raised against whole cells of isolate No. 2 showed the best sensitivity. With latex particles coated with this antibody, we could detect $5{\times}10^4$ CFU of A. hydrophila in 5 min. The cross-reactivity with bacteria constituting the normal intestinal microflora and other pathogens for rainbow trout was insignificant. This latex agglutination assay method produced positive reaction with all clinical isolates of A. hydrophila which were identified by species-specific PCR for 16S rRNA in A. hydrophila.

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The Effect of Corporate Taxpayer's Compliance with Tax Audit: A Case Study in Indonesia

  • MUKIYIDIN, Mukiyidin;MUDA, Iskandar;KHOLIS, Azizul
    • The Journal of Asian Finance, Economics and Business
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    • 제8권5호
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    • pp.717-725
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    • 2021
  • The study answers the awareness of taxpayers, tax authority services, tax socialization, tax collection, and account representative's controlling (monitoring) for compliance of the corporate Taxpayer. The study also examines the moderating effect of the tax audit to control the corporate Taxpayer's compliance. In this study, the method of non-probability sampling was used. The research data was gathered by handing out questionnaires at the Medan Kota Tax Office in Indonesia. The SEM method was used for data analysis through Lisrel 8.8 program. The findings of the study concluded that tax collection has a significant positive effect on corporate taxpayer's compliance and Tax audit moderates the relation between tax authority services and the account representative's controlling (monitoring) with corporate taxpayers' compliance. Also, tax socialization and account representative's controlling (monitoring) have a positive effect on the corporate taxpayer's compliance. In contrast, variables of taxpayer's awareness and tax authority services have a negative impact. Furthermore, tax audit does not moderate the relation between taxpayer awareness, tax socialization, and tax collection with the corporate taxpayer's compliance at Medan Kota Tax Office Indonesia. The findings suggest the Indonesian policymakers and tax authorities must create corporate legislation to handle compliance by leveraging the loopholes in tax legislation law and the tax audit system.

도커 기반의 실시간 데이터 연계 및 처리 환경을 고려한 빅데이터 관리 플랫폼 개발 (Development of Big-data Management Platform Considering Docker Based Real Time Data Connecting and Processing Environments)

  • 김동길;박용순;정태윤
    • 대한임베디드공학회논문지
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    • 제16권4호
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    • pp.153-161
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    • 2021
  • Real-time access is required to handle continuous and unstructured data and should be flexible in management under dynamic state. Platform can be built to allow data collection, storage, and processing from local-server or multi-server. Although the former centralize method is easy to control, it creates an overload problem because it proceeds all the processing in one unit, and the latter distributed method performs parallel processing, so it is fast to respond and can easily scale system capacity, but the design is complex. This paper provides data collection and processing on one platform to derive significant insights from various data held by an enterprise or agency in the latter manner, which is intuitively available on dashboards and utilizes Spark to improve distributed processing performance. All service utilize dockers to distribute and management. The data used in this study was 100% collected from Kafka, showing that when the file size is 4.4 gigabytes, the data processing speed in spark cluster mode is 2 minute 15 seconds, about 3 minutes 19 seconds faster than the local mode.

WIRELESS SENSOR NETWORK BASED BRIDGE MANAGEMENT SYSTEM FOR INFRASTRUCTURE ASSET MANAGEMENT

  • Jung-Yeol Kim;Myung-Jin Chae;Giu Lee;Jae-Woo Park;Moon-Young Cho
    • 국제학술발표논문집
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    • The 3th International Conference on Construction Engineering and Project Management
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    • pp.1324-1327
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    • 2009
  • Social infrastructure is the basis of public welfare and should be recognized and managed as important assets. Bridge is one of the most important infrastructures to be managed systematically because the impact of the failure is critical. It is essential to monitor the performance of bridges in order to manage them as an asset. But current analytical methods such as predictive modeling and structural analysis are very complicated and difficult to use in practice. To apply these methods, structural and material condition data collection should be performed in each element of bridge. But it is difficult to collect these detailed data in large numbers and various kinds of bridges. Therefore, it is necessary to collect data of major measurement items and predict the life of bridges roughly with advanced information technologies. When certain measurement items reach predefined limits in the monitoring bridges, precise performance measurement will be done by detailed site measurement. This paper describes the selection of major measurement items that can represent the tendency of bridge life and introduces automated bridge data collection test-bed using wireless sensor network technology. The following will be major parts of this paper: 1) Examining the features of conventional bridge management system and data collection method 2) Mileage concept as a bridge life indicator and measuring method of the indicator 3) Test-bed of automated and real-time based bridge life indicator monitoring system using wireless sensor network

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보건용 마스크의 분진포집효율, 흡기저항 및 CO2 농도 (Dust Collection Efficiency, Inhalation Pressure, and CO2 Concentration in Health Masks)

  • 한돈희;김일순
    • 한국환경보건학회지
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    • 제46권1호
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    • pp.78-87
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    • 2020
  • Objectives: To identify the degree of physical burden, a determination was undertaken of dust collection efficiency, inhalation pressure, and CO2 concentration related to health masks certified by the Ministry of Food and Drug Safety (MFDS). Methods: Twenty health masks were purchased on the market. Dust collection efficiency and inhalation pressure were determined in the same manner as in MFDS certification testing, respectively using TSI Model 8130 (TSI, U.S.) and ART Plus (Korea). CO2 concentrations for 20 subjects using a CO2 analyzer (G100, G150, Geotechnical Instrument Ltd., UK) were measured with a similar method as a total inward leakage test. In addition to CO2 levels, dead space volumes in the masks was determined for predicting concentrations of CO2 in inhalation air. Results: Most of the dust collection efficiencies found for the 20 masks were far higher than the standard. Four KF94s met KF99 and four KF80s even met KF94. Most inhalation pressures were also much lower than the standard, with many almost one-half of the standard. The mean and standard deviation of CO2 concentration in the mask were 2.9±0.44%. Considering dead volume, the prediction for CO2 concentration in the inhalation air was 4,395±1,266 ppm. Conclusions: For healthy men and women, the dust collection efficiency and inhalation pressure of health masks were not at a level that would affect their health. Although CO2 levels in the inhalation air were predicted not to affect health, research on the physiological effects of health masks on Koreans is needed for more precise research.

GIS를 활용한 단독주택지역의 음식물쓰레기 수거요소 분석연구 (Factor Estimation For Food Wastes Collection Using GIS)

  • 유기영;김부겸
    • 유기물자원화
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    • 제10권3호
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    • pp.92-97
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    • 2002
  • 본 연구에서는 표본지역의 음식물쓰레기 수거요소를 GIS를 활용하여 분석하였다. 음식물쓰레기 수거요소인 수거인력과 장비를 구하기 위해 표본지역인 S동을 대상으로 환경미화원의 수거이동속도(43m/min), 수거이동계수(1.9), 수거이동거리(17,200m/day), 수거가능량(0.313kg/day)을 측정하였다. 이때, 소요인원 분석을 위한 수거이동계수는 GIS의 활용을 통한 표본지역의 직선거리 분석으로 가능하였고 다른 지역으로의 확대 적용 역시 GIS활용을 통한 각 지역의 직선거리 분석으로 가능하였다. 분석결과, N자치구 단독주택지역의 음식물쓰레기 수거인원은 총 10인이 소요되었으나 실제적으로 4개 수거업체가 수거를 담당함으로써 총 11인이 소요되었고 수거차량 역시 총 7대가 소요되었으나 실제적으로는 4개 수거업체가 수거를 담당함으로써 총 8대가 소요되었다.

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Heterojunction Quantum Dot Solar Cells Based on Vertically Growth TiO2 Anatase Nanorod Arrays with Improved Charge Collection Property

  • Chung, Hyun Suk;Han, Gill Sang;Park, So Yeon;Lee, Dong Geon;Jung, Hyun Suk
    • 한국진공학회:학술대회논문집
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    • 한국진공학회 2014년도 제46회 동계 정기학술대회 초록집
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    • pp.466.2-466.2
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    • 2014
  • The Quantum dot (QD) solar cells have been under active research due to their high light harvesting efficiencies and low fabrication cost. In spite of these advantages, there have been some problems on the charge collection due to the limitation of the diffusion length. The modification of advanced nanostructure is capable of solving the charge collection problem by increasing diffusion length of electron. One dimensional nanomaterials such as nanorods, nanowires, and nanotubes may enhance charge collection efficiency in QD solar cells. In this study, we synthesized $TiO_2$ anatase nanorod arrays with length of 200 nm by two-step sol-gel method. The morphology and crystal structure for the nanorod were characterized by using scanning electron microscopy (SEM), transmission electron microscopy (TEM), and X-ray diffraction (XRD). The anatase nanorods are single-crystalline and possess preferred orientation along with (001) direction. The photovoltaic properties for the heterojunction structure QD solar cells based on the anatase nanorod were also characterized. Compared with conventional $TiO_2$ nanoparticle based QD solar cells, these nanostructure solar cells exhibited better charge collection properties due to long life time measured by transient open circuit studies. Our findings demonstrate that the single crystalline anatase nanorod arrays are promising charge transport semiconductors for heterojunction QD solar cells.

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