• 제목/요약/키워드: Collection 6

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고양이의 정액 채취 방법이 동결 정액의 생존성에 미치는 영향 (Effect of Semen Collection Methods on the Post-thaw Viability of Cat Semen)

  • 하아나;윤진호;김유곤;조아라;이경림;공일근
    • Reproductive and Developmental Biology
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    • 제35권1호
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    • pp.55-60
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    • 2011
  • The objective of this study was carried out to evaluate the efficiency of sperm collection methods on the post-thaw viability of cat semen. The cat semen was collected by artificial virginal (AV) and electronic ejaculate (EE) methods. The composition of semen extender was consisted of Tris-buffer supplemented with 20% egg yolk and 1% P/S antibiotics in Ext I, and more added 8% glycerol, 1.0% Equex STM paste of total volume in Ext II. The collected semen was adjusted the concentration and then diluted in Ext I for optimal concentration. The diluted semen was cooling to $5^{\circ}C$ temperature in refrigerator for at least 2 hrs and then diluted stepwise with Ext II for at least 1 hrs. After an equilibration for 1 hrs, the cooled semen was packaged in 0.5 ml straw and then freezing on the $LN_2$ vapor over 5 cm above from $LN_2$ and then immersed directly in $LN_2$ for cryopreservation. The frozen semen was thawed in $38^{\circ}C$ water for 15 sec and then evaluated the motility, viability, and morphology. Post-thaw semen were calculated the motility by SMI (sperm motility index). The live-dead sperm was evaluated by Eosin-B and morphological evaluation was by Diff-quik kit staining. The post-thaw concentration ($89{\times}10^6$ /ml vs. $128{\times}10^6$ /ml), viability ($22.6{\pm}10.6%$ vs. $37.1{\pm}26.1%$), morphological normality ($27.0{\pm}50.2%$ vs. $45.6{\pm}123.0%$) of EE and AV groups were not significant different, but the post-thaw motility was significant lower in EE than that in AV group ($53.1{\pm}3.6$ vs. $73.6{\pm}5.7$) (p<0.05). In conclusion, semen collection methods did not significant different between EE and AV groups except of post-thaw motility and so both semen collection methods could be applied in feline semen collection methods.

전기집진장치에서 가스의 물리적인 특성에 따른 포집구역내의 입자포집율 연구 (A Study on the Mass Collection Efficiency in Collector Step of Electrostatic Precipitator by Physical Gas Characterization)

  • 하상안;임경택;신남철
    • 한국환경과학회지
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    • 제7권1호
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    • pp.36-40
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    • 1998
  • This study was carried out to investigate the collection Efficiency of mass in collector step at the different of physical gas characterization. This work has focused on the dependence of the collection efficiency of mass in the collector zone of a two-stage set up field with gas temperature T and the dew point tmeperature. To identify the dependence of the mass collection efficiency on the Bounded plate of the collector zone MP.k by the spectre electric resistance of dust $p_e$. and the relative humidify ${\varphi}$, 20 at- tempts have been made with three different gas temperature ($50{\circ}C, 80{\circ}C, 110{\circ}C$) at different dew point. At the specific electric resistance of dust $p_e$=$10^6{\Omega}m$ which relative humidity corresponds to $\phi$ > 15%, a easy rise of the sounded plate secluded dust mass share was measured atwain. As the result of the higher cohesion imprisonment power due to the adsorbtion of particle, the rinse of the relative humidity developed on the particle surface. Therefore, the collection efficiency of mass was not predominant the high temperature T in the collector zone, neither was the pecific ellectric resistance of dust dependent.

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정전 방식 디젤 PM 포집 장치 성능 및 엔진 적용성 평가 (Evaluation on Performance of an Electrostatic Diesel PM Trap Device and Its Application to Diesel Engine After-treatment)

  • 김학준;한방우;김용진
    • 한국자동차공학회논문집
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    • 제16권6호
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    • pp.176-183
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    • 2008
  • Performance of electrostatic diesel PM filtration systems (E-DPS) with different types has been tested using the carbon particles generated by spark discharge in laboratory. Among the five electrostatic precipitators, the multiple wires cylindrical E-DPS with the highest collection efficiency and relatively lower differential pressure at the flow rate of $1\;m^3$/min, as an applicable device to diesel engine as an after treatment system, has been combined with another collection cylinder to improve the collection efficiency of diesel particulate matters generated from diesel engines. The multiple wires cylindrical E-DPS combined with the cylindrical collector showed the collection efficiency of more than 60% at the engine speed of 2,000 rpm with the engine loads of 25 and 50%.

주방환기용 그리스 필터의 입자포집 특성에 관한 수치해석 (A Numerical Study on the Particle Collection Characteristics of a Grease Filter for Kitchen Ventilation)

  • 김기정;배귀남;김영일;허남건
    • 설비공학논문집
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    • 제14권10호
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    • pp.792-800
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    • 2002
  • A grease filter is used to remove grease generated from a cooking appliance in a kitchen. This numerical study has been conducted to investigate the particle collection characteristics of a grease filter having nominal flowrate of $100m^3$/h. The flow field and particle trajectories in the grease filter with a flow chamber were simulated by using the commercial code of STAR-CD. The air velocity and pressure distributions were discussed in detail. The pressure drop of a grease filter rapidly increases with increasing the air flowrate. The numerical values of the pressure drop are slightly lower than the experimental values when the air flowrates are 50, 75, and 100㎥/h. The particle collection efficiency of a grease filter increases with increasing the particle diameter, the particle density, and the air flowrate, which means that the inertial impaction is a dominant particle removal mechanism in a grease filter. The cut-off diameter of the tested grease filter representing 50-% collection efficiency is about 11.6$\mu$m for water droplets at $100m^3$/h.

Perception of agricultural biotechnology according to information navigation activities on agricultural biotechnology

  • Bumkyu, Lee;Sung-Dug, Oh;Youn Sung, Cho
    • 농업과학연구
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    • 제48권4호
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    • pp.761-770
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    • 2021
  • A study was undertaken to identify variations in the level of awareness, the information collection status, the level of acceptance, and the characteristics of information collection as it pertains to agricultural biotechnology based on information collection in the field of agricultural biotechnology. When examining subjective awareness, objective awareness, and interest in agricultural biotechnology, the results showed that the levels of all three variables were higher in cases where information exploration of agricultural biotechnology had been experienced. Among the participants who have experience in voluntarily collecting information about agricultural biotechnology, helpful information at a rate of 51.6% and harmful information at a rate of 42.0% were acquired, values much higher than the 37% and 30% corresponding rates for participants who had no experience. However, it was also found that harmful information has a significantly greater impact on perceptions of the future. The characteristics of information collection for agricultural biotechnology indicated that those with experience in voluntary information collection particularly preferred the Internet, whereas those without any experience showed little difference with regard to television and/or the internet. According to a survey of participants' interest areas in agricultural biotechnology and their level of information acquisition, "Safety of human bodies" was rated the top interest area at 36.9%, which also had the highest level of information acquisition at 30.0%. In providing and sharing information in the future, this is a direct reference to the priorities and proportions of each field.

Direct Embryo Collection(DEC)에 의한 한우의 수정란 채란 (Embryo Recovery by Direct Embryo Collection (DEC) in Korean Native Cattle (Hanwoo))

  • 유한준;이용승;박정준;김기원;박춘근
    • 한국수정란이식학회지
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    • 제26권3호
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    • pp.153-158
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    • 2011
  • This study was performed in order to simplify the operation and minimize stress of donor and be readily available in the field with low cost and high quality embryos using the Direct Embryo Collection (DEC). Donors, at random stages of the estrous cycle, received a CIDR. 7 days later, 200 mg FSH was treated with 40, 30, 20, 10 mg FSH levels in declining doses twice daily by intramuscular injection for 4 days. On the 3rd day administration of FSH, 25 mg $PGF_2{\alpha}$ was administered and CIDR was withdrawn. After FSH injections were complete, donors were artificially inseminated twice at 12 hr intervals. The donor cattle received 250 ${\mu}g$ GnRH at time of 1 st insemination and embryos were recovered 8 days after the 1st insemination. Embryo collection from superovulated donors was performed to flushing by non-surgical methods of 3-way, 2-way and DEC (l-way). The average number of recovered embryos were 11.25${\pm}$0.63, 12.5${\pm}$0.65 and 11.75${\pm}$0.48 from operations of 3-way, 2-way and DEC methods, respectively. There were no significant differences among the embryo collection methods. Also, The average number of transferable embryos were 6.25${\pm}$0.48, 7.25${\pm}$0.48 and 7.25${\pm}$0.63 from each embryo collection procedures. The number of transferable embryos was no differences among the 3-way, 2-way and DEC methods, respectively. Meanwhile, the ratio of transferable embryos for all recovered embryos from DEC methods was higher as 61.7 % than 55.6 %, 58 % from methods of 3-way, 2-way. And the flushing solution required for recovering embryos by DEC method was significantly lower as 0.28${\pm}$0.32 1 than 1.8${\pm}$0.12 1, 1.75${\pm}$0.10 1 from 3-way, 2-way methods (p<0.05). Also, the time required for recovering embryos by DEC methods was significantly lower as 27${\pm}$2 min than 51${\pm}$3, 45${\pm}$2 min, respectively (p<0.05). In conclusion, these results suggest that DEC method for embryo collection may be effectively used for production of in vivo embryos using less flushing solution and, it might be effectively available in the field compared to conventional embryo recovery methods using 3-way or 2-way balloon catheter.

이미지 처리 알고리즘을 이용한 무인 천일염 포집장치의 색상 검출 성능 향상에 관한 연구 (A Study on the Improvement of Color Detection Performance of Unmanned Salt Collection Vehicles Using an Image Processing Algorithm)

  • 김선덕;안병원;박경민
    • 해양환경안전학회지
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    • 제28권6호
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    • pp.1054-1062
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    • 2022
  • 한국 천일염 생산 지역의 인구는 빠르게 고령화되고 있어 생산 노동자가 줄고 있는 추세이다. 소금 포집 작업은 천일염 생산과정에서 가장 많은 노동력을 필요로 한다. 기존의 포집 장치는 사람의 작동 및 운전이 필요하여 상당한 노동력이 필요해서, 천일염 무인포집장치를 개발하여 생산 노동자의 노동력을 감소시키고자 한다. 천일염 포집장치는 색상 검출을 통해 소금의 포집 상황과 염전에서의 위치를 파악하도록 설계되었기 때문에, 포집장치의 색상 검출 성능이 중요한 요소이다. 그래서 색상 검출 성능 향상을 위해 이미지 처리를 이용한 알고리즘을 연구하였다. 알고리즘은 입력 이미지를 크기 재조정, 회전 및 투시 변환을 이용하여 around-view 이미지를 생성하고, RoI를 설정하여 해당 영역만 HSV 색상 모델로 변환하고 논리곱 연산을 통해 색상 영역을 검출한다. 검출 된 색상영역은 형태학적 연산을 이용하여 검출 영역을 확장하고 노이즈를 제거하여 컨투어와 이미지 모멘트를 이용하여 검출영역의 면적을 계산하고 설정된 면적과 비교하여 염판에서 포집장치의 위치 경우를 결정한다. 성능 평가는 알고리즘을 적용한 최종 검출 색상의 계산 면적과 알고리즘의 각 단계의 검출 색상의 면적을 비교하여 평가하였다. 평가 결과 소금을 검출하는 흰색의 경우 최소 25%에서 최대 99% 이상, 빨간색의 경우 최소 44%에서 최대 68%, 파란색과 녹색은 평균적으로 각각 7%와 15% 검출면적 증가가 있어 색상 검출 성능이 향상되었음을 확인할 수 있었으며, 이를 무인 천일염 포집장치의 무인작업 수행을 위한 위치 확인에 적용 가능할 것으로 사료된다.

조류생산잠재력조사 방법개발에 의한 육수환경의 부영양화 방지대책에 관한 연구(I) -순수분리종의 형태 및 증식특성- (A Study on Protection Plan of Eutrophication in Fresh Water Environment by Development of Methods for Algal Growth Potential test (I) -Morphology and Growth Characteristics of Isolated algae-)

  • 위인선;나철호;이종빈;주현수
    • 한국환경보건학회지
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    • 제23권1호
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    • pp.18-27
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    • 1997
  • The isolation, morphological study and growth characteristics of the algae were investigated from Lake Chuam. The isolated algae were applied the Agal Growth Potential test. The method of isolation and purification of the algae were used to Agar plating(AP), nutrient enrichment(NE), dilution(DI) and micro capillary technique(MC). Total isolated algae were 21 species. They were composed of Cyanophyceae, Dinophyceae, Bacillariophyceae, Euglenophyceae and Chlorophyceae. The numbers of algal strain by isolation technique were highest in dilution(21 species), and those of the rests were showed in order of NE > MC > AP. The sizes of isolated Selenastrum and Scenedesmus were $1.8\pm 1.4 \mu m$, $3.3\pm 0.9 \mu m$ in diameter and $6.4\pm 2.3 \mu m$, $13.6\pm 1.9 \mu m$ in length respectively. The morphology of isolated algae and NIES-collection strain was very similar each other, but the size was smaller isolated algae than that of NIES-collection. The optimum culture condition of isolated Selenastrum and Scenedesmus was about 30$\circ$C(25$\circ$C-35$\circ$C) in temperature and the maximum growth was appeared between 7,000 lux and 8,000 lux in the light intensity. The comparison of $\mu$(specific growth rate) on the concentration of nutrients such as nitrate and phosphate, isolated Selenastrum was appeared maximum it at 1.0 mg $NO_3-N/l$ but NIES-collection strain was showed 95% of maximum it at same nitrate concentration. Maximum g of isolated algae and NIES-collection strain in Scenedesmus onto nitrate concentration were very similar with the result of selenastrum. The specific growth rates of isolated algae and NIES-collection strain on the gradient concentration of phosphate were showed 0.72/day and 0.70/day at 0.02 mg $PO_4-P/l$ in Selenastrum but those of Scenedesmus were appeared 0.61/day and 0.57/day at same concentration $PO_4-P$.

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Age of Data in Contemporary Research Articles Published in Representative General Radiology Journals

  • Kang, Ji Hun;Kim, Dong Hwan;Park, Seong Ho;Baek, Jung Hwan
    • Korean Journal of Radiology
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    • 제19권6호
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    • pp.1172-1178
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    • 2018
  • Objective: To analyze and compare the age of data in contemporary research articles published in representative general radiology journals. Materials and Methods: We searched for articles reporting original research studies analyzing patient data that were published in the print issues of the Korean Journal of Radiology (KJR), European Radiology (ER), and Radiology in 2017. Eligible articles were reviewed to extract data collection period (time from first patient recruitment to last patient follow-up) and age of data (time between data collection end and publication). The journals were compared in terms of the proportion of articles reporting the data collection period to the level of calendar month and regarding the age of data. Results: There were 50, 492, and 254 eligible articles in KJR, ER, and Radiology, respectively. Of these, 44 (88%; 95% confidence interval [CI]: 75.8-94.8%), 359 (73%; 95% CI: 68.9-76.7%), and 211 (83.1%; 95% CI: 78-87.2%) articles, respectively, provided enough details of data collection period, revealing a significant difference between ER and Radiology (p = 0.002). The age of data was significantly greater in KJR (median age: 826 days; range: 299-2843 days) than in ER (median age: 570 days; range: 56-4742 days; p < 0.001) and Radiology (median age: 618; range: 75-4271 days; p < 0.001). Conclusion: Korean Journal of Radiology did not fall behind ER or Radiology in reporting of data collection period, but showed a significantly greater age of data than ER and Radiology, suggesting that KJR should take measures to improve the timeliness of its data.

칼 라거펠트 디렉팅의 샤넬과 펜디에 대한 디자인 특성 연구 (A Study on Design Characteristics of Chanel's and Fendi's Collections under the Direction of Karl Lagerfeld)

  • 배우리;김윤경;이경희
    • 한국의류산업학회지
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    • 제23권6호
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    • pp.709-725
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    • 2021
  • The study focused on the design features of Chanel and Fendi, directed by Carl Lagerfeld, creative director of Chanel and Fendi until his recent death. The range of the study was from the 2017 S/S Collection to the 2019 F/W Collection, which collected a total of 767 fashion photographs, including 483 Chanel, 284 Fendi, with tops, bottoms and dresses at VOGUE (https://www.vogue.com). According to the data analysis criteria organized based on prior research and related literature, it was classified in the order of form, color, material, pattern, decoration, fashion image, item and coordination, and content analysis was conducted based on statistical analysis. Overall, the design characteristics of the Chanel collection, directed by Karl Lagerfeld, were rectangle form, tone in tone coloring, combination of identical materials, geometric patterns, and classical images as the main design characteristics of the Chanel collection. The design characteristics shown in the Fendi collection directed by Karl Lagerfeld were rectangle form, tone in tone coloration, hard material combination, abstract pattern, and total coordination. Comparing the design features of Chanel and Fendi, directed by Karl Lagerfeld, is as follows. Chanel and Fendi's designs show a lot of rectangle form, tone-in-tone colors, hard-materials and combination of the same material.