• Title/Summary/Keyword: Cold test

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Ecophysiological Changes in a Cold Tolerant Transgenic Tobacco Plant Containing a Zinc Finger Protein (PIF1) Gene

  • Yun, Sung-Chul;Kwon, Hawk-Bin
    • Korean Journal of Environmental Agriculture
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    • v.27 no.4
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    • pp.389-394
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    • 2008
  • The ecophysiological changes occurring upon cold stress were studied using cold tolerant transgenic and wild-type tobacco plants. In a previous study, cold tolerance in tobacco was induced by the introduction of a gene encoding the zinc finger transcription factor, PIF1. Gas-exchange measurements including net photosynthesis and stomatal conductance were performed prior to, in the middle of, and after a cold-stress treatment of $1{\pm}2^{\circ}C$ for 96 h in each of the four seasons. In both transgenic and wild-type plants, gas-exchange parameters were severely decreased in the middle of the cold treatment, but had recovered after 2-3 h of adaptation in a greenhouse. Most t-test comparisons on gas-exchange measurements between the two plant types did not show statistical significance. Wild-type plants had slightly more water-soaked damage on the leaves than the transgenic plants. A light-response curve did not show any differences between the two plant types. However, the curve for assimilation-internal $CO_2$ in wild-type plants showed a much higher slope than that of the PIF1 transgenic plants. This means that the wild-type plant is more capable of regenerating Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) and has greater electron transport capacity. In conclusion, cold-resistant transgenic tobacco plants demonstrated a better recovery of net photosynthesis and stomatal conductance after cold-stress treatment compared to wild-type plants, but the ecophysiological recoveries of the transgenic plants were not statistically significant.

Improvement of Abiotic Stress Resilience for Stable Rice Production

  • Dongjin Shin;Hyunggon Mang;Jiyun Lee
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2022.10a
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    • pp.13-13
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    • 2022
  • Recently, stable crop production is threatened by the effects of climate change. In particular, it is difficult to consistently maintain agricultural policies due to large price fluctuations depending on the difference in total domestic rice production from year to year. For stable rice production amid changes in the crop growing environment, development of varieties with improved disease resistance and abiotic stress stability is becoming more important. In here, drought and cold tolerant trait have been studied. First, for the development of drought tolerant varieties, we analyzed which agricultural traits are mainly affected by domestic drought conditions. As a result, it was observed that drought caused by the lack of water during transplanting season inhibits the development of the number of tiller and reduces the yield. 'Samgang' was selected as a useful genetic resource with strong drought tolerant and stable tiller number development even under drought conditions by phenotype screening. Three of drought tolerant QTLs were identified using doubled haploid (DH) population derived from a cross between Nacdong and Samgang, a drought sensitive and a tolerant, respectively. Among these QTLs, when qVDT2 and qVDTl1 were integrated, it was investigated that the tiller number development was relatively stable in the rainfed paddy field conditions. It is known that the high-yielding Tongil-type cultivars are severely affected by cold stress throughout the entire growth stage. In this study, we established conditions that can test the cold tolerance phenotype with alternate temperature to treat low temperatures in indoor growth conditions similar to those in field conditions at seedling stage. Three cold tolerant QTLs were explored using population derived from a cross between Hanareum2 (cold sensitive variety, Tongil-type) and Unkwang (cold tolerant variety, Japonica). Among these QTLs, qSCT12 showed strong cold tolerant phenotype, and when all of three QTLs were integrated, it was investigated that cold tolerant score was relatively similar to its donor parent, Unkwang, in our experimental conditions. We are performing that development of new variety with improved cold tolerant through the introduction of these QTLs.

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Evaluation of water-Soluble Lubricant for Cold Forging and Optimization of Coating Process (냉간단조용 수용성 윤활제의 평가 및 윤활 처리 공정의 최적화)

  • Lim, W.J.;Lee, I.S.;Je, J.S.;Ko, D.C.;Kim, B.M.
    • Proceedings of the Korean Society for Technology of Plasticity Conference
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    • 2007.05a
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    • pp.149-154
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    • 2007
  • The zinc prosphate film treatments used to lubricating treatment of mostly cold forging processes. But there are several problems happened to lubricating treatment process such as happening harmful environment on person, complex lubrication processing occurring in energy and time consumption, eco-destructive and chemical by-product generation, the needs of waste disposal etc. As a result, a water-soluble lubricant was developed to replace the perfect or some of the zinc prosphate film in the world. In order to solve these problems, this study evaluated the performance of the typical water-soluble. In this study, for these requirement inquiry of two part. First, about possibility of replace zinc phosphate lubricant, quantitatively evaluation developed of water-soluble lubricant for cold forging vs zinc phosphate lubricant. Second, About optimization of coating Process use to equipment with practicable automatic coating Process. The performance evaluation of these lubricants was conducted using the double cup extrusion test and spike forging test. With the use of the commercial FE code DEFORM, friction factor calibration curves, i.e. cup height ratio vs. punch stroke and spike height vs. punch stroke, were established for different friction factor values. By matching the cup height ratio and the punch stroke and spike height vs. punch stroke from experiment to that obtained from FE simulations, the friction factor of the lubricants was determined. Survey of comparative analysis use to SEM that sprayed lubricant surface structure of grain shape and characteristic of lubricant performance based on grain shape and deformed lubricant surface expansion. As a result, developed lubricant were found to perform comparable to or better than zinc phosphate. And thought this result, innovatively cope with generated problem of existing lubrication process.

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Fuel-Side Cold-Flow Test and Pressure Drop Analysis on Technology Demonstration Model of 75 ton-class Regeneratively-Cooled Combustion Chamber (75톤급 재생냉각 연소기 기술검증시제 연료 수류시험 및 차압 해석)

  • Ahn, Kyu-Bok;Kim, Jong-Gyu;Lim, Byoung-Jik;Kim, Mun-Ki;Kang, Dong-Hyuk;Kim, Seong-Ku;Choi, Hwan-Seok
    • Proceedings of the Korean Society of Propulsion Engineers Conference
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    • 2011.11a
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    • pp.807-812
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    • 2011
  • Fuel-side cold-flow tests were performed on the technology demonstration model of a 75 ton-class liquid rocket engine combustion chamber for the first stage of the Korea space launch vehicle II. Pressure drop in the cooling channels of the combustion chamber was measured by changing fuel mass flow rate through a pressure regulating system. Pressure drop in each segment of the chamber could be obtained and a lot of pressure drop was caused by high flow velocity in the nozzle throat segment. The accuracy of a hydraulic analysis method for calculating a pressure loss in cooling channels could be verified by applying it to the cold-flow test conditions.

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Middle-aged Consumers' Preferences for Clothing Images (중년 남녀 소비자의 선호 의복이미지에 관한 연구)

  • Chung, Sung-Jee
    • Journal of the Korea Fashion and Costume Design Association
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    • v.16 no.1
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    • pp.1-13
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    • 2014
  • The purposes of the study were to explore differences in preference for clothing images according to wearing situations and clothing product involvement. A questionnaire was developed by the researcher and was collected by 101 men and 100 women aged between 40 and 59. Data were analyzed by factor analysis, repeated measure ANOVA, t-test adjusted by Bonferroni, paired sample t-test, and independent sample t-test. The results of the study showed that clothing images were classified into five factors including hard cold image, modern urban image, bold unique image, and heavy luxurious image. There were significant differences in preference among clothing images according to wearing situations and clothing product involvement. While modern urbane image was most preferred, hard cold image was least preferred in both formal and informal wearing situations, and for either high or low involvement clothing products. Also, there were significant differences in clothing image preference according to wearing situations and product involvement. In formal situations, stronger preference for modern urban image and heavy luxurious image showed than in informal situations, and bold unique image and heavy luxurious image were more preferred for high involvement clothing product than for low involvement clothing product. In addition, there were significant differences in preferences for clothing images between groups according to gender and age. In both formal and informal situations, men showed stronger preferences than women for hard cold image, but women for bold unique image. For either high or low involvement clothing products, men preferred hard cold image, modern urban image and heavy luxurious image more than women, but women preferred bold unique image more than men. Lastly, for high involvement clothing product, subjects aged fifties showed stronger preferences than forties for modern urban image and heavy luxurious image, but for low involvement clothing product, modern urban image was more preferred.

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An effect of thermo-stimulation of lower abdomen on autonomic nervous system: An experimental study

  • Kim, Kyeong Han;Kim, Jong Uk;Kim, Bo Hyun;Shin, Jin Hyeon;Hong, Seong Jin;Lee, Sang Ryong;Yook, Tae-han
    • Journal of Pharmacopuncture
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    • v.21 no.2
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    • pp.98-103
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    • 2018
  • Objective: DIt is need to evaluate he changes of the bio-signals through the hot-cold stimulation in the CV4. Methods: The 30 healthy participants were enrolled and randomly allocated, to one of three groups(10 participants for each group): the hot stone therapy(HST) group, or the cold stone therapy(CST) group or no treatment group(NT). All the participants took a rest for 10 minutes for stability before the test. And additional 10 minute rest after measurements of skin test and sEMG. After that two group received hot or cold stone therapy for 30 minutes and one group treated nothing with HRV test. Results: HRV LF value showed a significant increase over time in all three groups, but there was no significant difference between groups but HRV HF value did not show any significant difference with time in all three groups and there was no significant difference between groups. sEMG value showed a significant increase in the left side of the masseter muscle of the HST group and sebum levels was a significant decreased in HST group but no significant meaning was found. Conclusion: There was no objective evidence that hot-cold stimulation produced bio-signals changes in comparison to the control group, but additional studies are needed as the subjects were limited.

Fuel-Side Cold-Flow Test and Pressure Drop Analysis on Technology Demonstration Model of 75 ton-class Regeneratively-Cooled Combustion Chamber (75톤급 재생냉각 연소기 기술검증시제 연료 수류시험 및 차압 해석)

  • Ahn, Kyubok;Kim, Jong-Gyu;Lim, Byoungjik;Kim, Munki;Kang, Donghyuk;Kim, Seong-Ku;Choi, Hwan-Seok
    • Journal of the Korean Society of Propulsion Engineers
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    • v.16 no.6
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    • pp.56-61
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    • 2012
  • Fuel-side cold-flow tests were performed on the technology demonstration model of a 75 ton-class liquid rocket engine combustion chamber for the first stage of the Korea space launch vehicle II. Pressure drop in the cooling channels of the combustion chamber was measured by changing fuel mass flow rate through a pressure regulating system. Pressure drop in each segment of the chamber could be obtained and a lot of pressure drop was caused by high flow velocity in the nozzle throat segment. The accuracy of a hydraulic analysis method for calculating a pressure loss in cooling channels could be verified by applying it to the cold-flow test conditions.

Germinability and Physiological Properties of Maize Seeds Affected by Matriconditioning (Matriconditioning에 의한 옥수수 종자 활력증진과 생리적 특성변화)

  • 이석순;서정문;윤상희;이문정
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.48 no.3
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    • pp.267-275
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    • 2003
  • To observe the effects of seed matriconditioning on the emergence, plumule growth, and seed physiological properties of normal and aged seeds of three corn(Zea mays L.) genotypes (dent, sugary, and shrunken-2), normal and artificially aged seeds were matriconditioned at 50, 75, 100, and 125% moisture contents of vermiculite at $25^{\circ}C$ for 24 hours and the germinability was examined at $25^{\circ}C$ and in cold test (7 days at 1$0^{\circ}C$followed by $25^{\circ}C$). Emergence rate, emergence speed, and plumule growth were smaller in the order of dent>sugary>shrunken-2. Matriconditioning did not affect the emergence rate of normal dent and sugary seeds, while it enhanced emergence speed and plumule growth. In contrast, the emergence rate, emergence speed, and plumule growth of normal shrunken-2 and aged seeds of all genotypes were enhanced as the moisture content of vermiculite increased up to 125% both at $25^{\circ}C$ and in cold test. Matriconditioning promoted $\alpha$-amylase activity and DNA and soluble protein contents, while it reduced the leakage of total sugars and electrolytes from the seeds when soaked in water.

Performance test of micronozzle (마이크로 노즐 성능평가)

  • Moon, Seong-Hwan;Oh, Hwa-Young;Huh, Hwan-Il
    • Journal of the Korean Society for Aeronautical & Space Sciences
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    • v.33 no.5
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    • pp.72-78
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    • 2005
  • We conducted the performance test of micronozzle having nozzle throat diameter of 1.0, 0.5, 0.25 mm in an ambient pressure. We used N2 gas as a cold gas propellant. We varied chamber pressure from 2 to 20 bar and measured the thrust and mass flow rate. Through the test, we concluded that viscous losses were increased with decreasing chamber pressure. We found that micronozzle performance was higher than orifice performance through thrust comparison.

The Turbocharger Cold Test Bench for NVH test (터보차져 NVH 시험장치)

  • Kim, Hyung-Jin;Choi, Sang-Bo;Kim, Jae-Heon;Kang, Koo-Tae
    • Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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    • 2007.11a
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    • pp.913-917
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    • 2007
  • The turbocharger for a vehicle is consisting of a centrifugal compressor and turbine. These compressor and turbine are vibrating and emitting noises through the T/C body, exhaust system (Catalyst, Bellows, Pipe, etc) and Intake system (Hoses, Intercooler pipes, Intercooler) as rotating. A turbocharger cold test bench is constructed to reduce these noises, especially for the purpose of realizing transient operating environment and oil temperature control to simulate the vehicle operating characteristics with intake system and exhaust system. This research laid the groundwork to develop a lower noise level T/C through understanding the mechanism of the noise source of T/C.

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