• Title/Summary/Keyword: Cold stress

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Comparison of structure, function and regulation of plant cold shock domain proteins to bacterial and animal cold shock domain proteins

  • Chaikam, Vijay;Karlson, Dale T.
    • BMB Reports
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    • v.43 no.1
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    • pp.1-8
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    • 2010
  • The cold shock domain (CSD) is among the most ancient and well conserved nucleic acid binding domains from bacteria to higher animals and plants. The CSD facilitates binding to RNA, ssDNA and dsDNA and most functions attributed to cold shock domain proteins are mediated by this nucleic acid binding activity. In prokaryotes, cold shock domain proteins only contain a single CSD and are termed cold shock proteins (Csps). In animal model systems, various auxiliary domains are present in addition to the CSD and are commonly named Y-box proteins. Similar to animal CSPs, plant CSPs contain auxiliary C-terminal domains in addition to their N-terminal CSD. Cold shock domain proteins have been shown to play important roles in development and stress adaptation in wide variety of organisms. In this review, the structure, function and regulation of plant CSPs are compared and contrasted to the characteristics of bacterial and animal CSPs.

Assessment of fatigue damage in 5% chrome cold rolling work roll (5% 크롬 냉간 압연용 작업롤의 피로손상 평가에 대한 연구)

  • Kwak, G.J.;Kim, K.S.;Lee, S.W.;Yeo, W.K.;Park, Y.C.
    • Proceedings of the KSME Conference
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    • 2000.11a
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    • pp.316-321
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    • 2000
  • The role of fatigue on the surface damage of 5% chrome cold milling work roll is evaluated. Uniaxial and torsional fatigue tests are carried out, and the baseline data for fatigue life evaluation are established. An appropriate multiaxial fatigue parameter is developed from the fatigue data. Fatigue tests are also performed under compressive mean stresses, and a mean stress model is formulated. A computer program is developed to assess the interaction of fatigue and grinding of the roll. The fatigue damage is computed for selected servicing conditions. It is found that the fatigue damage can be an important issue when the effect of mean stress is ignored, however the fatigue damage is negligibly small when the effect of mean stress including the residual stresses currently used is fully accounted. The result indicates that spalling due to the growth of thermal shock cracks is more important than fatigue damage in roll surface failure.

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Physiological Characteristics Related to Cold Injury in Rice (수도 냉해에 관련된 생리적 특성 고찰)

  • 석순종;허일봉;임정남
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.36 no.5
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    • pp.429-444
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    • 1991
  • Cold stress influence plant growth through a wide range of growth characters. Adverse effects of low temperature to plant growth come from results of colligative and complex physiological responses to cold stress. To evaluate more exactly cold tolerance of crop plant, it is needed to observe physiological changes induced by cold stress and to analyze relationships between intraspecific variations in physiological factors related to cold tolerance and the extent of cold tolerance in the field. Therefore, the composition and unsaturation ratio of fatty acids in phospholipid, a constituent of membrane, the transition-temperature in respiratory activity of mitochodria, the chlorophyll fluorescence as a factor related to photosynthesis were investigated in rice plant and data on these factors were compared with the degree of cold tolerance obtained in the field experiment. Also, effects of hardening and Mn++ treatment were evaluated as a method to reduce chilling injuries. The unsaturation ratio of fatty acids, whether rice plants were grown in a natural condition or under the chilling stress, was higher in the cold- tolerant varieties and was significantly correlated with the degree of cold tolerance (1-9) observed in the field experiment. And it was also increased by chilling treatment or hardening treatment, due to a reduction in palmitic acid content and an increase in linolenic acid content. The transition-temperature of respiratory activity of mitochodria isolated from etiolated rice seedlings ($25^{\circ}C$, two week-grown in the dark), was correlated with the degree of cold tolerance in the field, cold -tolerant varieties showing a lower transition-temperature. It was not influenced by growth stages. The intensity of chlorophyll fluorescence was highly correlated with the degree of cold tolerance, cold-tolerant varieties having a higher fluorescence intensity. By foliar application of Mn, the transition-temperature of respiratory activity was lowered as much as 0-2$^{\circ}C$ in all tested varieties. Soil application of Mn induced more significant effect in cold-susceptible varieties with a possibility of reducing chilling injuries. On the whole, there were high correlationships among the degree of cold tolerance, the unsaturation ratio of fatty acids in phospholipid, the transition- temperature of respiratory activity and chlorophyll fluorescence except for a few varieties. The transition- temperature of respiratory activity appeared to be negatively correlated with the unsaturation ratio of fatty acids. and the chlorophyll fluorescence to be positively correlated with the unsaturation ratio. This implies that these physical and physiological factors were very closely related to cold tolerance and can be used as an effective index of the evaluation of cold tolerance of crop plant. But other factors as well as three factors discussed above are needed to be considered colligatively and altogether with a systematic analysis for the more exact evaluation of cold tolerance. in rice cultivars. in rice cultivars.

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Effect of Cold Stress on Activities of Protein kinase C Subspecies in Rat Brain Regions

  • 이재란;최명언
    • Proceedings of the Korean Society of Applied Pharmacology
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    • 1994.04a
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    • pp.259-259
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    • 1994
  • Protein kinase C (PKC) participates in many cellular signal transduction. Previously we found that PKC activity of whole rat brain was altered after an exposure to cold temperature of 4 $^{\circ}C$ (Lee and Choi, Exp. Neurobiol., 2, 6, 1993). In this time PKC activity in each region of rat brain was investigated in order to know each regions is affected mostly by the stress.

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Mechanical Characteristics of Nano-Structured Tool Steel by Ultrasonic Cold Forging Technology

  • Suh, Chang-Min;Song, Gil-Ho;Suh, Min-Soo;Pyoun, Young-Shik;Kim, Min-Ho
    • Proceedings of the Korea Committee for Ocean Resources and Engineering Conference
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    • 2006.11a
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    • pp.35-40
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    • 2006
  • Ultrasonic cold forging technology (UCFT) utilizing ultrasonic vibration energy is a method to induce severe plastic deformation to a material surface, therefore the structure of the material surface becomes a nano-crystal structure from the surface to a certain depth. It improves the mechanical properties; hardness, compressive residual stress, wear and fatigue characteristics. Applying UCFT to a rolling process in the steel industry is introduced in this study. First, the UCFT specimens of a tool steel (SKD-61/equivalent H13) are prepared and tested to verify the effects of the UCFT in a variety of mechanical properties, the UCFT is applied to the trimming knives in a cold rolling process. It has been determined that UCFT improves the mechanical properties effectively and becomes a practical method to improve productivity and reliability by about two times compared with the conventionally treated tooling in the trimming process in a cold rolling line.

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Effects of Center Segregation on Weld Cold Cracking Susceptibility (용접 저온균열 감수성에 미치는 중심 편석의 영향)

  • 안영호;이종봉;장래웅;소문섭
    • Journal of Welding and Joining
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    • v.12 no.2
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    • pp.87-96
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    • 1994
  • Correlation between microstructural features and segregation of elements (Si, Mn, P and S) near the mid of thickness in the base metal and the synthetic HAZ was investigated. Furthermore, the relationship between the degree of center segregation and weld cold cracking susceptibility in the thickness direction was also conducted by evaluating the effect of P concentration on the critical applied stress. The results obtained are as follows: 1) Pearlite band, containing the MnS type inclusion and a locally transformed structure with a higher hardness, was observed in the center segregation region. 2) By the weld thermal cycle, center segregation region was transformed to the white band which had a higher hardness than that of base metal due to a greater hardenability of concentrated Mn, P etc.. 3) Weld cold cracking susceptibility in the thickness direction was mainly dependent on the concentration of impurity elements rather than on the number of the segregated particles near the mid of thickness. 4) During welding, the higher concentrated region was easily changed into white band. Therefore, it could be predicted that the initiation and propagation of a cold crack would be promoted by increasing the restraint stress and hydrogen content.

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Mold-design Verification of Ball Housing Insert Die in Non Processing Type Multi-stage Cold Forging (다단냉간단조 비가공 타입에서 볼하우징 인서트 다이의 금형설계 검증)

  • Hwang, Won-Seok;Choi, Jong-Won;Jung, Eu-Enn;Kang, Myungchang
    • Journal of the Korean Society of Manufacturing Process Engineers
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    • v.20 no.12
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    • pp.8-15
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    • 2021
  • Cold forging is a method in which molding is performed at room temperature. It has a high material recovery rate and dimensional precision and produces excellent surface quality, and it is mainly used for the production of bolted or housing products. The lifespan of cold forging molds is generally determined by the wear of the mold, plastic deformation of the mold, and fatigue strength. Cold forging molds are frequently damaged due to fatigue destruction rather than wear and plastic deformation in a high-temperature environment as it is molded at room temperature without preheating the raw material and mold. Based on the results analyzed through FEM, an effective mold structure design method was proposed by analyzing the changes in tensile and compressive stresses on molds according to the number of molds and reinforcement rings and comparing the product geometry and mold stress using three existing mold models.

Survival Factor Gene FgSvf1 Is Required for Normal Growth and Stress Resistance in Fusarium graminearum

  • Li, Taiying;Jung, Boknam;Park, Sook-Young;Lee, Jungkwan
    • The Plant Pathology Journal
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    • v.35 no.5
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    • pp.393-405
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    • 2019
  • Survival factor 1 (Svf1) is a protein involved in cell survival pathways. In Saccharomyces cerevisiae, Svf1 is required for the diauxic growth shift and survival under stress conditions. In this study, we characterized the role of FgSvf1, the Svf1 homolog in the homothallic ascomycete fungus Fusarium graminearum. In the FgSvf1 deletion mutant, conidial germination was delayed, vegetative growth was reduced, and pathogenicity was completely abolished. Although the FgSvf1 deletion mutant produced perithecia, the normal maturation of ascospore was dismissed in deletion mutant. The FgSvf1 deletion mutant also showed reduced resistance to osmotic, fungicide, and cold stress and reduced sensitivity to oxidative stress when compared to the wild-type strain. In addition, we showed that FgSvf1 affects glycolysis, which results in the abnormal vegetative growth in the FgSvf1 deletion mutant. Further, intracellular reactive oxygen species (ROS) accumulated in the FgSvf1 deletion mutant, and this accumulated ROS might be related to the reduced sensitivity to oxidative stress and the reduced resistance to cold stress and fungicide stress. Overall, understanding the role of FgSvf1 in F. graminearum provides a new target to control F. graminearum infections in fields.

The Role of Nail-fold Capillary Blood Velocity in Evaluating Cold Hypersensitivity in the Hands: A Pilot Study (수부냉증 진단을 위한 조갑모세혈관 혈류속도 측정 예비연구)

  • Ahn, Ilkoo;Park, Ki-Hyun;Lee, Siwoo;Jeong, Kyoungsik;Bae, Ji-yong;Kwon, Jung-yeon;Kong, Kyung-hwan;Go, Ho-yeon;Bae, Kwang-Ho
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.35 no.6
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    • pp.242-248
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    • 2021
  • This study aims to compare nail-fold capillary blood velocity (CBV) according to the presence or absence of cold hypersensitivity in the hands (CHH) using nail-fold capillaroscopy under cold stress conditions. Eleven participants were taken from the cohort study for observing long-term health status of the faculty of Semyung university. Seven of the participants displayed CHH while four showed signs of non-CHH. CBV between 0 and 60 seconds (S1) and between 240 and 300 seconds (S2) was measured under cold stress for 5 minutes, respectively, and the average value was used to compare non-CHH group and CHH group. Body mass index, Nogung (PC8) temperature and temperature difference between Nogung (PC8) and Hyeopbaek (LU4) were significantly lower in CHH group compared to non-CHH group. The S2-S1 CBV difference was negatively correlated with PC8 temperature and PC8-LU4 temperature, and positively correlated with cold pattern score and cold hypersensitivity visual analog scale. The CHH group had a lower CBV overall than non-CHH group, and the S2-S1 CBV difference was significantly higher in the CHH group than in non-CHH group. This study suggests that CBV measured by nail-fold capillarosopy might be a useful indicator of cold hypersensitivity properties.