• Horticultural Science & Technology
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• v.34 no.3
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• pp.372-382
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• 2016

In this study, we investigated the effect of seed collection stage and alternating temperature on the germination of Deutzia paniculata (Hydrangeaceae). The seeds were collected from naturally growing D. paniculata populations in Mt. Palgong, Gyeonsangbuk-do, Korea every two weeks over a three month period from August through October, 2014, totaling five collections. We examined seed characteristics such as seed size (length and width) and weight, and germination percentages for each collection stage under four different germination temperature regimes. We also examined germination after 30 and 60 days of cold moist stratification. Each collection phase had a different germination percentage and the lowest germination percentage was observed in the seeds from phase I (average GP of 15.7%). The germination percentage was similar at the later collection phases and in the higher temperatures suggesting that later collection times and higher temperatures are optimal for D. paniculata germination. We found that the optimum collection stage with high viability and germination percentage of for D. paniculata was during the middle of the September.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2019.10a
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• pp.44-44
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• 2019

Spring sowing of ginseng seeds often results in failure of seedling establishment. Storage condition during winter, sowing time, and seed treatment might effect on germination. Here we tested effects of temperature regimes of seed storage on spring sowing. Dehisced wet or dry ginseng seeds were stored at $2^{\circ}C$, $-2^{\circ}C$, $-3.5^{\circ}C$, or alternating temperature: at $2^{\circ}C$ until December, $-3.5^{\circ}C$ in January, and $2^{\circ}C$ in February, and sowed in March. In overall, emergence rate was dependent on storage temperature, and $-3.5^{\circ}C$ resulted poorest emergence than other conditions. Storage of wet seeds in alternating temperature resulted highest emergence rate. Seed dry also affected on emergence rate, while it was dependent on the storage temperature. In terms of growth, storage at $2^{\circ}C$ as wet seed resulted highest growth, and dried seeds resulted poorer growth than wet seeds. As a modification of alternating temperature, seeds were stored at $2^{\circ}C$ at first, then transferred to $-3.5^{\circ}C$ at Nov 30, Dec 20, and Jan 10, each. When transfer date was delayed, emergence rate was increased. We suggest that seed storage temperature for ginseng should not be decreased below $-2^{\circ}C$, and alternative temperature regime for successful spring sowing could be useful.

• Horticultural Science & Technology
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• v.29 no.4
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• pp.352-357
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• 2011

Rose seed shows low germination percentages (about 20%) because it has a high amount of substances that inhibit germination in the pericarp. We investigated the effect of orientation of achene, the day after pollination (DAP), and cold storage with or without half-cut in achene for the germination percentage in R. rugosa. Germination percentages of intact or half-cut achenes were investigated in a 16-hour photoperiod at $25^{\circ}C$ room on basal MS medium for two weeks. In germination percentage, maximum 100% was measured within one week when half-cut achenes were cultured on an orientation that the embryos facing to the light. Half-cut achenes at 90 DAP were germinated 100% regardless of cold storage. Various LED lights (red, blue, yellow, green, and white) were illuminated over the half-cut achenes to gain the effect of light color. Germination percentage of R. rugosa seeds under blue LED reached the greatest with 90% within one week of culture and these seedlings were the best with a steady growth rate. It is concluded that half-cut achenes would be an effective method to improve seed germination in R. rugosa without stratification or scarification. This system could be applied to breeding studies in rose cultivars.

• Proceedings of the National Institute of Ecology of the Republic of Korea
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• v.2 no.4
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• pp.229-234
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• 2021

Iris dichotoma Pall. found on Daechung Island in Korea has been designated as an endangered species. To aid in conservation efforts of this species, this study investigated its germination characteristics and seed dormancy type. Four sets of seeds were incubated at different temperatures (4/1℃, 15/6℃, 20/10℃, and 25/15℃). One set of seeds was cold stratified (4 weeks at 4/1℃). The final germination rate and mean germination time showed that the optimal germination temperature was 25/15℃. Final germination rates were ~70%, showing no significant difference among temperature treatments. However, mean germination time were significantly different among all temperature treatments except for 4/1℃. Mean germination time for seeds with temperature treatments of 15/6℃, 20/10℃, and 25/15℃ were 3.2, 2.1, and 1.5 weeks, respectively. At 25/15℃, the mean germination time was half of that at 15/6℃. Seeds of I. dichotoma had fully developed embryos at the time of dispersal. No additional growth of the embryo was observed. Cold stratification did not affect the final germination rate or the mean germination time. This study shows that seeds of I. dichotoma have no physiological or morphological dormancy, unlike other members of the Iris genus known to have seed dormancy that needs a relatively high incubation temperature (≥25/15℃) for mass propagation to occur. These results will be useful for understanding ecophysiological mechanisms related to the species' habitat. They are also useful for mass propagation of I. dichotoma for the purpose of conserving this endangered species.

• Journal of Korean Society of Forest Science
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• v.86 no.4
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• pp.443-449
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• 1997

We examined the effect of pretreatments on seed germination, seedling growth, optimal seedling density of Phellodendron amurense. The seeds were carefully purified after collection in late Oct. from natural population at Mt. Chiri. For the evaluation of pretreatment effects, seeds were sowed in an experimental nursery after being stored in open ground($2{\sim}8^{\circ}C$) with soaking in 20% $H_2O_2$, 50-fold pon-pon solution, cold moist stratification in refrigerator($2{\sim}4^{\circ}C$) and dry storage in roam temperature($4{\sim}18^{\circ}C$), respectively, For the effect of growth density of seedlings, seedlings germinated in nursery were precisely controlled with 53, 104, 220, 380 seedlings per $m^2$. Among pretreatments for the promotion of germination rate, pon-pon treatment(68.8%) was highest, the others were higher than that in dry storage(2.3%), and these seed pretreatment methods were significantly different at 1% level. The height growth of seedlings showed the most vigor from Jun.12 to Jul. 8, and from Jul.20 to Aug. 2, and it reached to 72.8% of height growth. In case of 1-0 seedling, although seedling density per $m^2$ was increased, seedling growth was decreased. The optimal seedling density was 104 seedlings per $m^2$. The standard seedlings for plantation were above height 55cm, root collar diameter 8.1mm, and root length 21cm. These results demonstrated that seed of P. amurense requires stratification for the germination promotion.

• Korean Journal of Microbiology
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• v.45 no.2
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• pp.119-125
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• 2009

To understand the ecological function of heterotrophic bacterial community in water column of large freshwater lakes in the permafrost zone, we investigated the structure and function of bacterial community in Lake Khuvsgul, Mongolia. Species composition of overall bacterial community was analyzed by denaturing gradient gel electrophoresis (DGGE) of 16S rRNA gene fragments, and bacteria that can be cultured at 10oC were isolated and characterized. Based on the depth profile of environmental parameters, thermocline and chemocline were recognized at the 5~10 m zone of the water column. The stratified DGGE profile indicated that the discontinuity of water properties might influence the structure of bacterial community: band profiles in the 0~5 m zone were diverse with large change by depth, but the profile was relatively stable at the $\geq$10 m zone, with predominance of the band identified as Acidovorax facilis. Bacterial cultures were screened for protease, cellulase, amylase and lipase activity, and 23 isolates were selected for high activity of the hydrolytic enzymes. The isolates were identified based on their 16S rRNA gene sequences. In the surface water (zero meter depth), Acidovorax defluvii and Sphingobacterium faecium with high cellulase activity were present. Flavobacterium succinicans, Mycoplana bullata and A. facilis were stably predominant isolates at 2 m, 5 m, and $\geq$10 m depths, respectively. F. succinicans isolates showed high protease activity while M. bullata isolates showed moderate levels of protease and celluase activity. A. facilis isolates showed either cellulase or lipase activity, exclusively to each other. According to the profile of growth rates of the isolates in the temperature range of $0\sim42^{\circ}C$, the surface-zone (0~5 m) isolates were facultative psychrophiles while isolates from $\geq$10 m depth were typical mesophiles. This stratification is believed to be due to stratified availability of organic materials to the bacterial decomposers. In the water column below the chemoline, the environment is extremely oligotrophic so that the trait of rapid growth in low temperature might not be demanded by deep-lake decomposers. The stratified distribution of community composition and decomposer activity in Lake Khuvsgul implies that ecological functions of bacterial community in lakes of cold region are sharply divided by water column stratification.

• Korean Journal of Medicinal Crop Science
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• v.6 no.4
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• pp.258-264
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• 1998

This study was conducted to establish mass propagation system from seeds of Lavandula angustiolia Mill. Only 4% of dry seeds were germinated when they were incubated in $4^{\circ}C$. Germination rate of stratified seeds for 9 weeks was 15% higher in light than darkness. Soaking with 1000mg/l of $GA_3$ was helpful to overcome the effect of darkness. The optimal temperature for germination was $25^{\circ}C$ for the seeds that were treated with$GA_3$, solution. For the improvement of germination rates, pretreatment of $GA_3$ at 500-2000mg/l showed about 75% of germination, and in the combination treatment of $GA_3$, and BA, germination rate increased by about 10% in the treatment of 1000mg/l $GA_3+10mg/l$ BA compared with the 1000mg/l $GA_3$ treatment. Cold stratification treatment was very effective for seed germination, and over 70% of seeds were germinated when they were incubated in $4^{\circ}C$ for 9-12 weeks. In addition, there was synergic effect on the seed germination subject to stratification and 1000mg/l $GA_3$, treatment for 6 months. In the mixture of vermiculite: peatmoss (1 : 1, v/v), emergence rate was 6.7% in control and 65% in 1000mg/l $GA_3$ treatment, respectively.

• Journal of the Korean Association of Geographic Information Studies
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• v.8 no.3
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• pp.142-149
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• 2005

To provide observed oceanography data at coastal fish and shellfish farm in the northeastern sea of the Korean peninsula on real time base, we developed real time application system for fisheries oceanography information. The system has been made up a mooring buoy system, a server for oceanography data collection, a server for archiving data and a database system, and a web server for providing fisheries oceanography information using internet. Futhermore, to support letters service on a cellular phone, we developed the communication system from mooring buoy to cell phone on real time base. The oceanography data derived from the system are water temperature speed and direction of current in surface layer middle layer and bottom layer in hour. We were able to quantify short term variation of ocean conditions within several days at shellfish farm such as a scallop sea farm using our system. To reduce damages of fish and shellfish farm from abnormal phenomena of ocean conditions such as a broken stratification of water, an occurrence of abnormal coastal cold water and warm water we will be able to move vertically and horizontally the sea farm facilities to proper conditions using real time oceanography information derive from the system.

• Current Research on Agriculture and Life Sciences
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• v.7
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• pp.41-46
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• 1989

The seeds of Malus sieboldii were collected in late fall of 1988 and stored in cold stratification condition for 15 weeks. Growth regulators such as $GA_3$, BA and ABA were treated to the seed to know germination capacity and seedling growth. The results were as follows. 1) Higher germination was achieved in $GA_3$ and BA plots, But ABA treatment showed lower germination percentage and physiological dwarf and also induced to the secondary dormancy condition in partly. 2) Average length of time for germination was delayed over 2 days in ABA plot when comparing with the other plots. 3) Average length of plumule and radicle also was inhibited by ABA treatment. This means lower seedling vigor. 4) Fresh and dry weight of the seeding were higher in BA plot while ABA plot was lower and undesirable seedling was produced. From above mentioned results, it is believed that ABA treatment to the seed induced the secondary dormancy and physiological dwarf pattern. Therefore, utilization of BA can be produced the normal seedling, the authors believed.

• Journal of Korean Society of Forest Science
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• v.82 no.3
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• pp.227-234
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• 1993

This study was carried out to seek for a most effective hastening method of seed germination by Pon-Pon treatment and asexual multiplication method by cutting in Zanthozylum piperitum. The results obtained in this study were as follows : 1. Cold moist stratification under the ground accompanied with Pon-Pon treatment was shown 74.3% of seed germination, which was the most effective one among pretreatment methods with significance at 1% level. 2. Height growth was shown continuous growth pattern and the maximum growth was shown during the period 1 July to 31 July, which corresponds 40.3% of total annual growth. 3. Although no noticeable difference among the kind of plant growth regulator in the rooting rate, there was difference among concentrations with significance at 1% level. 4. In the case of number of primary roots and T/R rate of rooted cuttings, treatment of plant growth regulators was significantly superior to control. 5. T/R rate in rooted cuttings was a high negative correlation (r=-0.7164) with the number of primary roots.