• Title/Summary/Keyword: Cold stratification

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Embryo Culture of Taxus wallichiana (Zucc.)

  • Datta Mukul Manjari;Jha Sumita
    • Journal of Plant Biotechnology
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    • v.6 no.4
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    • pp.213-219
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    • 2004
  • Zygotic embryos were excised from immature and mature seeds of the Himalayan yew, Taxus wallichiana. The embryos germinated precociously when kept in darkness for 5 weeks and developed into full seedlings within 10-12 weeks. The highest rate of embryo germination ($81\%$) was obtained in modified Lloyd & McCown' s woody plant medium containing macro and micronutrients at half strength supplemented with $1\%$ activated charcoal, which supported both the best embryonic growth ($43\%$) and seedling development ($32\%$). However, the supplementation of basal media with kinetin, thidiazuron, 6-benzyl aminopurine or $GA_3$ had no effect on the germination of the embryos. The embryos derived from immature seeds germinated but the frequency of embryonic growth was better in mature seeds. Stratification of seeds effected precocious germination of embryos. Seeds kept at $4^{\circ}C$ for 1 week germinated earlier and at a higher frequency irrespective of the stage of seed maturity, while the germination rate declined with prolonged cold treatment for 1 month at that same temperature. Analysis of taxanes in germinating seedlings revealed that root tissues contained high levels of taxol, 10-deacetyl-baccatin ill and baccatin ill as compared to shoots. Thus embryo culture technique appears to overcome the lengthy dormancy requirement of T. wallichiana seeds.

Study on TES system application for industrial production facility (축냉시스템의 산업용 생산설비 적용에 대한 고찰)

  • Park, C.H.;Hong, S.S.;Kim, J.R.;Park, S.S.;Hwang, H.S.
    • Proceedings of the SAREK Conference
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    • 2009.06a
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    • pp.1288-1293
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    • 2009
  • The TES (Thermal Energy Storage) cooling system utilizing cheaper off-peak electricity has been applied just for building air-conditioning currently and causes limitation of usage rate and inefficiency of national resources utilization. In this regard, more says the necessity to apply TES system in industrial cooling system which is longer using period and wider usage. In this study, we will approve the technical and economical improvement in efficiency of industrial cooling system applied TES system by utilizing cheaper off-peak electricity and it will attribute the promotion of TES system and stabilization of supply and demand of electric power by proving the necessity to develop more efficient industrial cooling system by combining TES system.

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The Variation of Water Temperature and the Mass Mortalities of Sea Squirt, Halocynthia roretzi along Gyeongbuk Coasts of the East Sea in Summer, 2006. (2006년 하계 경북 연안의 수온변화와 우렁쉥이 폐사)

  • Lee, Yong-Hwa;Shim, Jeong-Min;Kim, Young-Suk;Hwang, Jae-Dong;Yoon, Suk-Hyun;Lee, Chu;Jin, Hyun-Gook
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.13 no.4
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    • pp.15-19
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    • 2007
  • Mass mortalities of the farmed sea squirt, Halocynthia roretzi, occurred in August, 2006 along the Gyeongbuk coasts of the East Sea without any pathological symptom. Suspecting the No. 10 typhoon, Wukong, which had passed over the relevant locations in the middle of August, 2006 as a culprit of the mortalities, we compared water temperature stratifications of the affected coasts before and after the typhoon. Just prior to the typhoon, cold waters lower than $15^{\circ}C$ were stratified at depths deeper than 10m probably by a persisting upwelling of cold bottom waters. The typhoon enforced water off-coast waters into coasts for 5 days, replacing the cold waters at depths $10{\sim}20m$ which can be justified by Ekman Spiral Effect. The results were an increment of water temperature by $10^{\circ}C$ and more at depths where sea squirts were suspended. The sudden elevation of water temperature might drive the sea squirts under abnormal stress to mass mortality.

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Species Composition and Spatial Distribution of Euphausiids of the Yellow Sea and Relationships with Environmental Factors

  • Yoon, Won-Duk;Yang, Joon-Yong;Lim, Dong-Hyun;Cho, Sung-Hwan;Park, Gyung-Soo
    • Ocean Science Journal
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    • v.41 no.1
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    • pp.11-29
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    • 2006
  • We investigated species composition and spatial distribution of the euphausiid community in the Yellow Sea and identified the relationship with environmental factors (temperature, salinity, chlorophyll $\alpha$, nitrate, phosphate, and silicate) using bimonthly data from June, 1997 to April, 1998. The environment varied during the sampling period. In warm seasons, thermocline was well developed rendering lower temperature and higher salinity and nutrient concentrations in the bottom layer. During cold seasons the water column was well mixed and no such vertical stratification was noted. Horizontal distribution of temperature, however, differed slightly between near-coast and offshore areas because of the shallow depth of the Yellow Sea, and between southern and northern areas because of the intrusion of water masses such as Yellow Sea Warm Current and Changjiang River Diluted Water. Four euphausiid species were identified: Euphausia pacifica, E. sanzoi, Pseudeuphausia sp. and Stylocheron affine. E. sanzoi and S. affine were collected, just one juvenile each, from the southern area in June and December, respectively. Pseudeuphausia sp. were collected in the eastern area all the year round except June. E. pacifica occurred at the whole study area and were the predominant species, representing at least 97.6% of the euphausiid abundance. Further, the distribution pattern of the species was varied in regards to developmental stages (adult, furcilia, calyptopis, egg). From spring to fall, E. pacifica adults were abundant in the central area where the Yellow Sea Bottom Cold Water prevailed. Furcilia and calyptopis extended their distribution into nearly all the study area during the same period. From late fall to winter, adults were found at the near-coastal are a with similar pattern for furcilia and calyptopis. The distribution pattern of E. pacifica was consistent regarding temperature, salinity, and three nutrients during the sampling period, whereas chlorophyll $\alpha$ showed a different pattern according to the developmental stages. The nutrients should indirectly affect via chlorophyll $\alpha$ and phytoplankton concentration. With respect to these results, we presented a scenario about how the environmental factors along with the water current affect the distribution of E. pacifica in the Yellow Sea.

Seasonal Variations of Water Quality in the Coastal Sea of Jungmun Resort Complex in Jeiu Island (제주도 중문관광단지 연안해역 수질의 계절변동)

  • Jang Seung-Min;Choi Young-Chan
    • Journal of the Korean Society for Marine Environment & Energy
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    • v.5 no.2
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    • pp.3-18
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    • 2002
  • This study has been carried out to find the water Quality in coastal sea of fungmun area, southern Jeju Island. In-situ observations and water sampling had been made every month from July 1997 to June 2000. The distributions of water temperature and salinity over the study area have been 13.8~27.0℃ and 30.0~34.7‰, respectively. Salinity is showed low salinity from June to September (rainy season) because of rain. Tsushima Warm Waters (TWW) as ≥15℃ and ≥34‰ influence the adjacent sea around Jeju Island all year round. Yangtse Coastal Waters (YCW) influence the surface layer around Jeju from June to September and so strong stratification (termocline, halocline) resulted at the depth of between 20~30m at outer-sea. However the stratification does not happen even in summer at inner-sea, which seem to be caused due to vertical mixing by wind, waves and tides. A water mass of high value of water temperature and salinity (respectively 14.1~17.7℃, 33.9~34.1‰) stayed at the lower layer in outer-sea all the year round. It is probably formed by mixing between TWW and YSBCW(Yellow Sea Bottom Cold Water). The mean value of DO was the lowest in summer and the highest in winter. COD and TH were the highest in summer and the lowest in winter. However, TP showed the lowest value in summer season, because the mean value of N/P ratio was over 16. The mean of N/P ratio was under 16 in other seasons. The phosphate would be a limiting factor in the growth of phytoplanHon in summer. Nitrate would be a limiting factor in other seasons. Distribution of chlorophyll a did not show any seasonal change in the study period, but especially increased during April and May in the first year(1998) and the second year(1999) all over the study area, which suggested that phytoplankton inhabitation distributed widely in the study area. The space averaged values were the highest for TIN in rainy season and lower for TP in rainy season than in other seasons. It suggests that river runoff influences the inner-sea.

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Temporal and Spatial Variations of Temperature and Salinity around Ganjeol Point in the Southeast Coast of Korea (한국 남동해 간절곶 주변해역의 열염구조와 시공간적 변동 특성)

  • Choo, Hyo-Sang;Jang, Duck-Jong
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.20 no.5
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    • pp.474-485
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    • 2014
  • Temporal and spatial variations of temperature and salinity around Ganjeol Point during January, April, August and November 2011 were studied using the data from CTD observations and temperature monitoring buoys deployed at 20 stations in the southeast coast of Korea. Temperature and salinity were nearly homogeneous through the whole depth by mixing of the seawater in spring and winter related to the sea surface cooling. Stratification induced by the river runoff and the bottom cold water was clear in summer. In autumn, sea water had vertical mixing initiated from surface layer and weak stratification at the middle and bottom layers. Low temperature and high salinity emerged throughout the year near Ganjeol Point, which inferred from turbulent mixing and upwelling by its topographical effect. Major periods of 1/4~1.4 day temperature fluctuations were recorded for the most part of the stations. According to the cross spectral density analysis, high coherence and small time lag for temperature fluctuation between layers were shown at Ganjeol Point. However, those features at the northen area of Hoeya river were opposed to Ganjeol Point. From analyses, thermohaline structure and its fluctuation around Ganjeol Point were characterized into those three parts, the south of Ganjeol Point, Ganjeol Point and the north of Ganjeol Point.

Effect mechanism of unfrozen water on the frozen soil-structure interface during the freezing-thawing process

  • Tang, Liyun;Du, Yang;Liu, Lang;Jin, Long;Yang, Liujun;Li, Guoyu
    • Geomechanics and Engineering
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    • v.22 no.3
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    • pp.245-254
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    • 2020
  • The interaction between the frozen soil and building structures deteriorates with the increasing temperature. A nuclear magnetic resonance (NMR) stratification test was conducted with respect to the unfrozen water content on the interface and a shear test was conducted on the frozen soil-structure interface to explore the shear characteristics of the frozen soil-structure interface and its failure mechanism during the thawing process. The test results showed that the unfrozen water at the interface during the thawing process can be clearly distributed in three stages, i.e., freezing, phase transition, and thawing, and that the shear strength of the interface decreases as the unfrozen water content increases. The internal friction angle and cohesive force display a change law of "as one falls, the other rises," and the minimum internal friction angle and maximum cohesive force can be observed at -1℃. In addition, the change characteristics of the interface strength parameters during the freezing process were compared, and the differences between the interface shear characteristics and failure mechanisms during the frozen soil-structure interface freezing-thawing process were discussed. The shear strength parameters of the interface was subjected to different changes during the freezing-thawing process because of the different interaction mechanisms of the molecular structures of ice and water in case of the ice-water phase transition of the test sample during the freezing-thawing process.

A Study on the Wild Exochorda serratifolia for Landscape Horticuitural Cultivation (야생 가침박탈(Exochorda serratifolia)의 조경원예화에 관한 연구)

  • 이기선;한교필;박원조;김일섭
    • Journal of the Korean Institute of Landscape Architecture
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    • v.15 no.2
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    • pp.139-148
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    • 1987
  • Exochorda serratifolia, broad-leaved shrub has beautiful flowers and is resistant to cold, shade and decease. New this native plant is considered to be worth being exploited as the outstanding plant for landscaping and horticulture. So this study was executed to utilize Exochorda serratifolia as the planting material for landscaping and horticulture through the survey of its habitat environment and the experimennt of its seed physiology and germination, vegetative propagation, culture and utilization, etc.. The results are as follows ; 1. The color of the flower is white, blooming in the early and middle of May and the seeds ripen late in Sep.. 2. The elevation, elevation, gradient and direction of the native habitat were 250m, 20-25$^{\circ}$and northern side respectively. 3. The soil pH of the natural habitat was 5.3 and soil fertility was poor. 4. Exochorda serratifolia appeared as indicator within P. densiflora community and its neighboring species were Q. mongolica, Q. dentata, R. mucronulatum, L. obtusiloba, E. oxyphillus, C. heterolphylla, var thunbergii, etc.. 5. The optimum temperature for seed germination was found at 20$^{\circ}C$ and the longer the stratification period at 5$^{\circ}C$ was, the lower the germination rate was. 6. The treat merits of GA and Kinetin increased the seed germination rate, especially under the dark condition but their high concentrations decreased the seed germination rate. 7. The rooted rate of the greenwood cutting was the highest at IBA 100ppm plot of vermiculite bed but its high concentration decreased the rooted rate conspicuously. 8. In the tissue culture, the each 1.0 ppd plot of NAA, Kinetin and NAA 1.0ppm + Kinetin showed the best growth. And the mixture of NAA 1.0ppm and Kinetin showed better growth than the single treatment of NAA or Kinetin did. 9. Transplanted Exochorda serratifolia showed healthy growth with shaded environmental condition(42.1% light intensity), therefore they can be cultivated as a shade tolerant landscape plant. 10. It was considered that Exochorda serratifolia was applicable to group planting at shade places or under trees in parks, homes, etc..

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Observations of the Cheju Current

  • Suk, Moon-Sik;Pang, Ig-Chan;Teague, William J.;Chang, Kyung-Il
    • Journal of the korean society of oceanography
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    • v.35 no.3
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    • pp.129-152
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    • 2000
  • The Cheju Current (CC), defined here as a mean eastward flow in the Cheju Strait, mostly carries water of high temperature and salinity originating from the Kuroshio in winter and spring, the Cheju Warm Current Water (CWCW). The strong core of the eastward component of the CC is found close to Cheju Island (Cheju-Do, hereafter) in winter and spring with a peak speed of about 17.0 cm/s. The eastward flow weakens towards the northern Cheju Strait, and a weak westward flow occurs occasionally close to the southern coast of Korea. The volume transport ranges from 0.37 to 0.45 Sv(1 Sv=10$^6$ m$^3$/s) in winter and spring. Seasonal thermocline and harocline are formed in summer and eroded in November. The occurrence of the CWCW is confined in the southern Cheju Strait close to Cheju-Do below the seasonal thermocline in summer and fall, and cold water occupies the lower layer north of the CWCW which is thought to be brought into the area from the area west of Cheju-Do along with the CWCW. Stratification acts to increase both the speed of the CC with a peak speed of greater than 30 cm/s and the vertical shear of the along-strait currents. The strong core of the CC detached from the coast of Cheju-Do and shifted to the north during the stratified seasons. The volume transport in summer and fall ranges 0.510.66 Sv, which is about 1.5 times larger than that in winter and spring. An annual cycle of the cross-strait sea level difference shows its maximum in summer and fall and minimum in winter and spring, whose tendency is consistent with the annual variability of the CC and its transport estimated from the ADCP measurements. Moored current measurements west of Cheju-Do indicate the clockwise turning of the CC, and the moored current measurements in the Cheju Strait for 1530 days show the low-frequency variability of the along-strait flow with a period of about 37 days.

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Recruitment characteristics of jack mackerel, Trachurus japonicus, in the waters around the Geumo Islands by using both sides fyke nets (각망을 이용한 금오열도 주변해역 전갱이의 연안가입특성 연구)

  • Kim, Hee-Yong;Choi, Mun-Seong;Seo, Young-Il;Lee, Sun-Kil;Cha, Hyung-Kee
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.47 no.4
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    • pp.356-368
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    • 2011
  • Species composition of fishes and the recruitment properties of jack mackerel, Trachurus japonicus, in the waters around the Geumo Islands in the mid-South Sea were investigated by using both sides fyke nets every month from February to December 2010. During study period, a total of 30,503 fishes (1,380.4 kg) were collected and classified into 2 classes, 16 orders, 61 families and 121 species. The dominant species was jack mackerel occupying 80.5% of total individuals and 44.4% of total biomass. The fork length range of jack mackerel was 5.5-26.8 cm and individuals about 6 cm was appeared only in the middle and southern area of the Geumo Islands in May. The new recruitment of jack mackerel appeared from May in the waters around the Geumo Islands is probably caused by the warm water intrusion associated with the development of stratification due to the extinction of seasonal coastal cold waters by the increase of solar radiation heat. Furthermore, the jack mackerels less than 6 cm recruiting in the mid-South Sea in spring were considered as mixed ones by individuals spawned in the East China Sea and in the waters around the Jeju Island considering the collected time, migration period and spawning time of them.